Search Results (8)

Lichens are symbiotic organisms with unique secondary metabolism. Various metabolites from lichens have shown antimicrobial activity. Nevertheless, very few studies have investigated the antimicrobial potential of the volatile compounds they produce. This study investigates the chemical composition and antimicrobial properties of volatile compounds from Pseudevernia furfuracea collected in two regions of Morocco. Hydrodistillation was used to obtain volatile compounds from samples collected in the High Atlas and Middle Atlas. Gas chromatography–mass spectrometry (GC-MS) analysis identified phenolic cyclic compounds as the primary constituents, with atraric acid and chloroatranol being the most abundant. Additionally, eight compounds were detected in lichens for the first time. The antimicrobial activity of these compounds was assessed using disc diffusion and broth microdilution methods. Both samples demonstrated significant antimicrobial effects against multidrug-resistant human bacteria, reference microorganisms, fish pathogens, and Candida albicans, with minimum inhibitory concentrations (MICs) ranging from 1000 µg/mL to 31.25 µg/mL. This study provides the first report on the volatile compounds from Pseudevernia furfuracea and their antimicrobial effects, particularly against fish pathogens, suggesting their potential as novel antimicrobial agents for human and veterinary use. Further research is warranted to explore these findings in more detail. Full article

Cigarette smoke is one of the main factors in Chronic Obstructive Pulmonary Disease (COPD), a respiratory syndrome marked by persistent respiratory symptoms and increasing airway obstruction. Perturbed NAD+/NADH levels may play a role in various diseases, including lung disorders like COPD. In our study, we investigated the preventive effect of NADH supplementation in an experimental model of COPD induced by cigarette smoke extract (CSE). N = 64 mice randomly distributed in eight groups were injected with NADH (two doses of 100 mg/kg or 200 mg/kg) or dexamethasone (2 mg/kg) before being exposed to CSE for up to 9 weeks. Additionally, NADH supplementation preserved lung antioxidant defenses by preventing the functional loss of key enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase, and the expression levels of glutathione (GSH) (n = 4, p < 0.001). It also reduced oxidative damage markers, such as malondialdehyde (MDA) and nitrites (n = 4, p < 0.001). A marked increase in tissue myeloperoxidase activity was assessed (MPO), confirming neutrophils implication in the inflammatory process. The latter was significantly ameliorated in the NADH-treated groups (p < 0.001). Finally, NADH prevented the CSE-induced secretion of cytokines such as Tumor Necrosis Factor alpha (TNF-α), IL-17, and IFN-y (n = 4, p < 0.001). Our study shows, for the first time, the clinical potential of NADH supplementation in preventing key features of COPD via its unique anti-inflammatory and antioxidant properties. Full article

Breast cancer (BC) is the most frequently diagnosed cancer and the leading cause of cancer death in women worldwide, accounting for 24.5% of total new cancer cases and 15.5% of total cancer deaths. Similarly, BC is the most common cancer among Moroccan women, comprising a noteworthy percentage of 40% of all cancers in women. Globally, 15% of cancers are attributable to infections; among them, viruses play a significant role. The present study aimed to explore the presence of a wide range of viral DNA in samples recovered from 76 Moroccan patients with BC and 12 controls using Luminex technology. The explored viruses were as follows: 10 polyomaviruses (PyVs): BKV, KIV, JCV, MCV, WUV, TSV, HPyV6, HPyV7, HPyV9, and SV40; and 5 Herpesviruses (HHVs): CMV, EBV1, EBV2, HSV1, and HSV2. Our results revealed the presence of PyVs DNA in both control (16.7%) and BC tissues (18.4%). Nonetheless, HHV DNA was detected exclusively in BC tissues (23.7%), with a predominance of Epstein–Barr virus (EBV) (21%). In conclusion, our study highlights the presence of EBV in human BC tissues, which may play an important role in its development and/or progression. Further investigations are needed to confirm the presence/co-presence of these viruses in BC. Full article

The mining industry manages large volumes of tailings, sludge, and residues that represent a huge environmental issue. This fact has prompted research into valorization of these wastes as alternative aggregates for concrete production, embankments, pavement material, etc. The use of mining wastes as a resource for construction presents two benefits: conserving natural resources and reducing the environmental impacts of mining. In the case of road construction, the use of mining wastes has not yet been developed on a large scale and there is a major lack of specific legislation. This gap is due to the variety of exploited rocks, the diversity of tailings, mine residues, or valuable by-products slated for valorization, and the environmental specifics. This paper presents a review on recycling mine wastes as road construction material, including waste rock and mine tailings. Those materials were mostly used in infrastructure where soils had initially poor geotechnical properties (low bearing capacity, frost susceptibility, swelling risk, etc.). Different mining wastes were used directly or stabilized by a hydraulic binder through geopolymerization or, in some cases, with bituminous treatment. Overall, the use of mine wastes for road construction will have a considerable environmental impact by reducing the volume of waste and offering sustainable raw materials. Full article

The phosphate extraction and processing has followed a traditional linear consumption model, where wastes are continuously produced and surface land is filled in rock piles. Thus, to promote a circular economy in a mining context, more eco-friendly and sustainable solutions at the regional level are needed. This paper deals with the potential utilization of phosphate screening waste rock 0–100 mm (SWR) as alternative aggregates for pavement applications. Detailed in situ and laboratory tests have been made for SWR characterization, and the practical modalities of implementation have been defined. The findings proved that SWR (10–100 mm) materials can be successfully used without treatment in capping layer construction for low-volume traffic pavement projects. Due to its high apatite content, the remaining fraction (0–10 mm) can be exploited to recover the residual phosphate using the processing method that is currently followed by the mining company. Furthermore, the environmental investigations showed that SWR does not present any potential contamination risk to the surrounding environment. The economic feasibility analysis confirmed the workability of SWR reuse in a radius of 100 km around their dumps due to its lower cost compared to conventional aggregates. This simple amendment may ensure a smooth transition from a current linear extractive approach to a circular economy. Full article

This paper deals with the potential reuse of coal mine waste rocks (CMWR) as an alternative material for road construction to conserve the natural resources and sustainable management of mining waste. The investigation was conducted through the determination of the chemical, mineralogical, geotechnical properties, and acid mine drainage formulation of CMWR as well as economic feasibility. This waste was used either alone for embankments and mixed with stabilizing agents fly ash (FA) and hydraulic road binder (HRB) for pavement applications. The experimental results confirmed that weathered CMWR can be successfully used alone as a sustainable alternative material for the embankment. Furthermore, the use of stabilizing agents in the following ratio CMWR:FA:HRB = 80:20:5 allow the use of CMWR in road sub-base layers for high-traffic pavements. Also, the environmental investigations showed that CMWR does not present any potential contaminating risk on the surrounding environment and most of the pyrite particles were already oxidized. Therefore, the environmental impact of acid mine drainage produced by pyritic waste throughout its life cycle can be neglected. Finally, an economic case study confirmed the workability of CMWR reuse in a radius of 29 km around their dumps by resulting in a lower cost compared with conventional materials. Full article

The road construction sector is a worldwide high consumer of natural aggregates. The use of unusual industrial by-products in road techniques can contribute to the conservation of non-renewable natural resources and the reduction of wastes produced by some industries. Phosphate waste rocks could be considered as potential alternative secondary raw materials in road construction. The use and valorization of these wastes is currently limited according to the Moroccan guide for road earthworks (GMTR). The guide has classified these materials as waste products, which consequently, cannot be used in road construction. However, phosphate waste rocks are sedimentary natural rocks which have not been subjected to any transformation other than mechanical fragmentation. The goal of this paper is to discuss key-properties of various phosphate mine waste rocks (PMWR) to be used as road materials. Samples were taken from different stockpiles in the phosphate mine site of Gantour in Morocco. The different waste rocks samples were characterized in terms of their physical, geotechnical, chemical, mineralogical and environmental properties using international testing norms. The obtained results showed that the studied PMWR presented satisfying characteristics; the specific (particle) density: ρs > 26 kN/m³, Los Angeles abrasion: 45% < LA < 58%), methylene blue value MBV < 1 g/100g, organic matter: OM < 1% and plasticity index: PI < 20%. All PMWR were confirmed as possessing the requested geotechnical properties to be used as materials for embankments. Moreover, leaching tests showed that none of them released any contaminants. In field application, these materials have been also successfully used in in situ experimental pilot testing. Therefore, the PMWR have to be classified in the category of natural aggregates that are similar to conventional materials. Full article

Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3–5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey’s method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC₅₀ values ranging between 0.1 and 6.7 µg/mL. Full article