Search Results (6)

In chili cultivation, obstacles to continuous cropping significantly compromise crop yield and soil health, whereas crop rotation can enhance the microbial environment of the soil and reduce disease incidence. However, its effects on the diversity of rhizosphere soil microbial communities are not clear. In this study, we analyzed the composition and characteristics of rhizosphere soil microbial communities under chili continuous cropping (CC) and chili–cotton crop rotation (CR) using high-throughput sequencing technology. CR treatment reduced the alpha diversity indices (including Chao1, Observed_species, and Shannon index) of bacterial communities and had less of an effect on fungal community diversity. Principal component analysis (PCA) revealed distinct compositional differences in bacterial and fungal communities between the treatments. Compared with CC, CR treatment has altered the structure of the soil microbial community. In terms of bacterial communities, the relative abundance of Firmicutes increased from 12.89% to 17.97%, while the Proteobacteria increased by 6.8%. At the genus level, CR treatment significantly enriched beneficial genera such as RB41 (8.19%), Lactobacillus (4.56%), and Bacillus (1.50%) (p < 0.05). In contrast, the relative abundances of Alternaria and Fusarium in the fungal community decreased by 6.62% and 5.34%, respectively (p < 0.05). Venn diagrams and linear discriminant effect size analysis (LEfSe) further indicated that CR facilitated the enrichment of beneficial bacteria, such as Bacillus, whereas CC favored enrichment of pathogens, such as Firmicutes. Fusarium solani MG6 and F. oxysporum LG2 are the primary chili root-rot pathogens. Optimal growth occurs at 25 °C, pH 6: after 5 days, MG6 colonies reach 6.42 ± 0.04 cm, and LG2 5.33 ± 0.02 cm, peaking in sporulation (p < 0.05). In addition, there are significant differences in the utilization spectra of carbon and nitrogen sources between the two strains of fungi, suggesting their different ecological adaptability. Integrated analyses revealed that CR enhanced soil health and reduced the root rot incidence by optimizing the structure of soil microbial communities, increasing the proportion of beneficial bacteria, and suppressing pathogens, providing a scientific basis for microbial-based soil management strategies in chili cultivation. Full article

Maize tassels are critical phenotypic organs in maize, and their quantity is essential for determining tasseling stages, estimating yield potential, monitoring growth status, and supporting crop breeding programs. However, tassel identification in complex field environments presents significant challenges due to occlusion, variable lighting conditions, multi-scale target complexities, and the asynchronous and irregular growth patterns characteristic of maize tassels. In response to these challenges, this paper presents a DMSF-YOLO model for maize tassel detection. In the network’s backbone front, conventional convolutions are replaced with conditional parameter convolutions (CondConv) to enhance feature extraction capabilities. A novel DMSF-P2 network architecture is designed, including a multi-scale fusion module (SSFF-D), a scale-splicing module (TFE), and a small object detection layer (P2), which further enhances the model’s feature fusion capabilities. By integrating a dynamic detection head (Dyhead), superior recognition accuracy for maize tassels across various scales is achieved. Additionally, the Wise-IoU loss function is used to improve localization precision and strengthen the model’s adaptability. Experimental results demonstrate that on our self-built maize tassel detection dataset, the proposed DMSF-YOLO model shows remarkable superiority compared with the baseline YOLOv8n model, with precision (P), recall (R), $mA{P}_{50}$, and $mA{P}_{50:95}$ increasing by 0.5%, 3.4%, 2.4%, and 3.9%, respectively. This approach enables accurate and reliable maize tassel detection in complex field environments, providing effective technical support for precision field management of maize crops. Full article

Background: Restless legs syndrome (RLS) is a common sensorimotor sleep disorder that affects sleep quality of life. Much effort has been made to make progress in RLS pharmacotherapy; however, patients with RLS still report poor long-term symptom control. Methods: Comprehensive Mendelian randomization (MR) was performed to search for potential causal genes and drug targets using the cis-pQTL and RLS GWAS data. Robustness was validated using the summary-based Mendelian randomization (SMR) method and co-localization analysis. Further evidence of pleiotropy of the target genes and their potential side effects was provided by phenome-wide MR analysis (MR-PheWAS). Finally, molecular docking simulations were conducted on drug candidates corresponding to these targets, which revealed promising binding affinities and interaction patterns and underscored the druggable potential of the target gene. All of the analyses above were conducted in the context of Homo sapiens. Results: MAN1A2 showed a statistically significant result in the MR analysis, which was validated through SMR and co-localization analysis. The MR-PheWAS showed a low probability of pleiotropy and prospective side effects. Molecular docking was used to visualize the binding structure and fine affinity for MAN1A2 and the drugs predicted by DSigDB. Conclusions: Our study provides comprehensive evidence supporting MAN1A2 as a promising causal gene and therapeutic target for RLS, offering insights into the underlying molecular mechanisms and paving the way for future drug development efforts. Full article

The limited computation resource of the centralized controller and communication bandwidth between the control and data planes become the bottleneck in forwarding the packets in Software-Defined Networking (SDN). Denial of Service (DoS) attacks based on Transmission Control Protocol (TCP) can exhaust the resources of the control plane and overload the infrastructure of SDN networks. To mitigate TCP DoS attacks, DoSDefender is proposed as an efficient kernel-mode TCP DoS prevention framework in the data plane for SDN. It can prevent TCP DoS attacks from entering SDN by verifying the validity of the attempts to establish a TCP connection from the source, migrating the connection, and relaying the packets between the source and the destination in kernel space. DoSDefender conforms to the de facto standard SDN protocol, the OpenFlow policy, which requires no additional devices and no modifications in the control plane. Experimental results show that DoSDefender can effectively prevent TCP DoS attacks in low computing consumption while maintaining low connection delay and high packet forwarding throughput. Full article

Building energy consumption prediction plays an important role in improving the energy utilization rate through helping building managers to make better decisions. However, as a result of randomness and noisy disturbance, it is not an easy task to realize accurate prediction of the building energy consumption. In order to obtain better building energy consumption prediction accuracy, an extreme deep learning approach is presented in this paper. The proposed approach combines stacked autoencoders (SAEs) with the extreme learning machine (ELM) to take advantage of their respective characteristics. In this proposed approach, the SAE is used to extract the building energy consumption features, while the ELM is utilized as a predictor to obtain accurate prediction results. To determine the input variables of the extreme deep learning model, the partial autocorrelation analysis method is adopted. Additionally, in order to examine the performances of the proposed approach, it is compared with some popular machine learning methods, such as the backward propagation neural network (BPNN), support vector regression (SVR), the generalized radial basis function neural network (GRBFNN) and multiple linear regression (MLR). Experimental results demonstrate that the proposed method has the best prediction performance in different cases of the building energy consumption. Full article

A series of sulfonium halides bearing allyl groups have been prepared andcharacterized. Anion metathesis with Li[Tf₂N] and Ag[N(CN)₂] resulted in sulfonium-basedionic liquids which exhibit low viscosities at room temperature. The solid state structure ofone of the halide salts was determined by single crystal X-ray diffraction. Full article