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Authors = Deanira Patrone

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15 pages, 1351 KiB  
Review
Cell Immortalization: In Vivo Molecular Bases and In Vitro Techniques for Obtention
by Javier Curi de Bardet, Celeste Ramírez Cardentey, Belkis López González, Deanira Patrone, Idania Lores Mulet, Dario Siniscalco and María de los Angeles Robinson-Agramonte
BioTech 2023, 12(1), 14; https://doi.org/10.3390/biotech12010014 - 28 Jan 2023
Cited by 17 | Viewed by 7731
Abstract
Somatic human cells can divide a finite number of times, a phenomenon known as the Hayflick limit. It is based on the progressive erosion of the telomeric ends each time the cell completes a replicative cycle. Given this problem, researchers need cell lines [...] Read more.
Somatic human cells can divide a finite number of times, a phenomenon known as the Hayflick limit. It is based on the progressive erosion of the telomeric ends each time the cell completes a replicative cycle. Given this problem, researchers need cell lines that do not enter the senescence phase after a certain number of divisions. In this way, more lasting studies can be carried out over time and avoid the tedious work involved in performing cell passes to fresh media. However, some cells have a high replicative potential, such as embryonic stem cells and cancer cells. To accomplish this, these cells express the enzyme telomerase or activate the mechanisms of alternative telomere elongation, which favors the maintenance of the length of their stable telomeres. Researchers have been able to develop cell immortalization technology by studying the cellular and molecular bases of both mechanisms and the genes involved in the control of the cell cycle. Through it, cells with infinite replicative capacity are obtained. To obtain them, viral oncogenes/oncoproteins, myc genes, ectopic expression of telomerase, and the manipulation of genes that regulate the cell cycle, such as p53 and Rb, have been used. Full article
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6 pages, 2314 KiB  
Protocol
Optimization of Peripheral Blood Mononuclear Cell Extraction from Small Volume of Blood Samples: Potential Implications for Children-Related Diseases
by Deanira Patrone, Nicola Alessio, Nicola Antonucci, Anna Lisa Brigida, Gianfranco Peluso, Umberto Galderisi and Dario Siniscalco
Methods Protoc. 2022, 5(2), 20; https://doi.org/10.3390/mps5020020 - 24 Feb 2022
Cited by 5 | Viewed by 10188
Abstract
Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an important cellular model to study immune responses and drug toxicity. The monocytic [...] Read more.
Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an important cellular model to study immune responses and drug toxicity. The monocytic cells, a fraction of PBMC, are strongly involved in some pathophysiological processes, such as inflammation and immune system changes. Here, we propose a simple, reliable protocol for obtaining peripheral blood-derived mononuclear cells from small volumes of blood samples. Full article
(This article belongs to the Section Molecular and Cellular Biology)
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