Search Results (3)

The complete blood count (CBC) is a highly requested test that is generally restricted to centralized laboratories, which are limited by high cost, being maintenance-demanding, and requiring costly equipment. The Hilab System (HS) is a small, handheld hematological platform that uses microscopy and chromatography techniques, combined with machine learning (ML) and artificial intelligence (AI), to perform a CBC test. This platform uses ML and AI techniques to add higher accuracy and reliability to the results besides allowing for faster reporting. For clinical and flagging capability evaluation of the handheld device, the study analyzed 550 blood samples of patients from a reference institution for oncological diseases. The clinical analysis encompassed the data comparison between the Hilab System and a conventional hematological analyzer (Sysmex XE-2100) for all CBC analytes. The flagging capability study compared the microscopic findings from the Hilab System and the standard blood smear evaluation method. The study also assessed the sample collection source (venous or capillary) influences. The Pearson correlation, Student t-test, Bland–Altman, and Passing–Bablok plot of analytes were calculated and are shown. Data from both methodologies were similar (p > 0.05; r ≥ 0.9 for most parameters) for all CBC analytes and flagging parameters. Venous and capillary samples did not differ statistically (p > 0.05). The study indicates that the Hilab System provides humanized blood collection associated with fast and accurate data, essential features for patient wellbeing and quick physician decision making. Full article

The land surface is an important component of numerical weather and climate forecast models due to their effect on energy–water balances and fluxes, and it is essential for forecasts on a seasonal scale. The present study aimed to understand the effects of land surface processes on initialization of seasonal forecasts in the austral spring, in particular soil moisture. We built forecasts with the Brazilian global Atmospheric Model hindcast from 2000 to 2010, with a configuration similar to those used in the operational environment. To improve it, we developed a new initial condition of the land surface using the Land Information System over South America and the Global Land Data Assimilation System for the rest of the globe and used it as the input in the forecast model. The results demonstrated that the model is sensitive to changes in soil moisture and that the new high–resolution soil moisture dataset can be used in model initialization, which resulted in an increase in the correlation of precipitation over part of South America. We also noticed an improvement in the representation of surface fluxes and an increase in soil moisture content and specific humidity at medium and low levels of the atmosphere. The analysis of the coupling between the land surface and the atmosphere showed that, for Central Brazil, the states of the continental surface define the surface fluxes. For the Amazon and La Plata Basins, the model did not correctly represent the coupling because it underestimated the soil moisture content. Full article

Long noncoding RNAs (lncRNAs) undergo splicing and have multiple transcribed isoforms. Nevertheless, for lncRNAs, as well as for mRNA, measurements of expression are routinely performed only at the gene level. Metformin is the first-line oral therapy for type 2 diabetes mellitus and other metabolic diseases. However, its mechanism of action remains not thoroughly explained. Transcriptomic analyses using metformin in different cell types reveal that only protein-coding genes are considered. We aimed to characterize lncRNA isoforms that were differentially affected by metformin treatment on multiple human cell types (three cancer, two non-cancer) and to provide insights into the lncRNA regulation by this drug. We selected six series to perform a differential expression (DE) isoform analysis. We also inferred the biological roles for lncRNA DE isoforms using in silico tools. We found the same isoform of an lncRNA (AC016831.6-205) highly expressed in all six metformin series, which has a second exon putatively coding for a peptide with relevance to the drug action. Moreover, the other two lncRNA isoforms (ZBED5-AS1-207 and AC125807.2-201) may also behave as cis-regulatory elements to the expression of transcripts in their vicinity. Our results strongly reinforce the importance of considering DE isoforms of lncRNA for understanding metformin mechanisms at the molecular level. Full article