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Search Results (7)

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Authors = Anastasia Kourti ORCID = 0000-0002-1677-222X

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28 pages, 10448 KiB  
Article
Ultrastructural and Immunohistochemical Study of Double and Combined Intravitreal Administration of Antifungal Agents in the Retina of New Zealand Albino Rabbits: An Experimental Protocol
by Sofia Karachrysafi, Maria Kourti, Sophia Tsokkou, Despoina Ioannou, Evangelia Kofidou, Georgios Delis, Sotiris Sotiriou, Athanasios Karamitsos, Maria Xioteli, Ioanna Dori, Penelope Anastasiadou, Ioannis Konstantinidis, Dimitrios Kavvadas, Fotios Chatzinikolaou, Anastasia Komnenou, Vasileios Karampatakis, Antonia Sioga and Theodora Papamitsou
J. Fungi 2025, 11(8), 564; https://doi.org/10.3390/jof11080564 - 29 Jul 2025
Viewed by 703
Abstract
Introduction: Fungal endophthalmitis (FE) is a rare but serious intraocular inflammatory disorder, resulting from an infection of the vitreous cavity from either endogenous or exogenous components that ultimately results in blindness. This current research study aims to elucidate the histological effects of the [...] Read more.
Introduction: Fungal endophthalmitis (FE) is a rare but serious intraocular inflammatory disorder, resulting from an infection of the vitreous cavity from either endogenous or exogenous components that ultimately results in blindness. This current research study aims to elucidate the histological effects of the intravitreal injection of the maximum safe dosage of voriconazole and micafungin on the retina and investigate potential histological alterations after the double and combined administration of voriconazole and micafungin. Methodology: Nine New Zealand Albino Rabbits were randomly assigned into three groups (V2, M2, and VM), and in each, voriconazole, micafungin, and a combination of the two medications were administered respectively. After the administration of the antifungal agents, the animals were sacrificed and their retinas were retrieved and studied under optical and electron microscopes. The immunohistochemical markers TNF-a and IL6 were also studied. Results: TNF-a was positive in the VM group, as it was found to be mildly positive in the presence of apoptotic cells in the ganglion cell layer. Conclusions: This study revealed voriconazole has a greater toxicity in a multi-dosage administration. However, micafungin revealed a greater toxicity than voriconazole from the extent of the lesions observed. Full article
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17 pages, 562 KiB  
Review
Oxidative Stress and Its Role in the Emergence and Progression of Myelodysplastic Syndromes: Insights from Proteomic Analysis and Other Methodologies
by Anastasia Boura-Theodorou, Konstantina Psatha, Stefania Maniatsi, Areti Kourti, Georgia Kaiafa, Michalis Aivaliotis and Kali Makedou
Proteomes 2025, 13(2), 21; https://doi.org/10.3390/proteomes13020021 - 3 Jun 2025
Cited by 1 | Viewed by 1090
Abstract
Myelodysplastic syndromes (MDS) belong to a category of malignant stem-cell and myeloid disorders that deteriorate the function of the hematopoietic system exacerbated by the omnipresent anemia that characterizes myelodysplasia. The pathogenesis of MDS is driven by cytogenetic abnormalities along with the excessive production [...] Read more.
Myelodysplastic syndromes (MDS) belong to a category of malignant stem-cell and myeloid disorders that deteriorate the function of the hematopoietic system exacerbated by the omnipresent anemia that characterizes myelodysplasia. The pathogenesis of MDS is driven by cytogenetic abnormalities along with the excessive production of pro-inflammatory cytokines and disruptions in inflammatory signaling pathway, particularly through the influence of carbonylated proteins, which are linked to MDS progression. An additional and major contributor to the pathogenesis of MDS is oxidative stress marked by uncontrolled levels of reactive oxygen species (ROS), which have been suggested as potential biomarkers for assessing disease severity and stratifying MDS cases throughout a variety of methods. Excessive and non-accumulative levels of free iron can also lead to iron overload (IOL)—related promotion of a high oxidative state, whether we refer to treatment-related IOL or natural IOL mechanisms. Proteomic analysis has emerged as a powerful tool for profiling protein samples, and, consequently, understanding the molecular changes underlying MDS. In this review, we evaluated studies and their methodologies aiming in investigating distinctive proteomics signatures associated with MDS pathogenesis, focusing on the role of oxidative stress at the protein level. Full article
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18 pages, 375 KiB  
Review
Development of a Holistic In Vitro Cell-Free Approach to Determine the Redox Bioactivity of Agricultural Products
by Zoi Skaperda, Fotios Tekos, Periklis Vardakas, Paraskevi-Maria Nechalioti, Maria Kourti, Anastasia Patouna, Sotiria Makri, Maria Gkasdrogka and Demetrios Kouretas
Int. J. Mol. Sci. 2023, 24(22), 16447; https://doi.org/10.3390/ijms242216447 - 17 Nov 2023
Viewed by 1937
Abstract
In recent years, there has been a strong consumer demand for food products that provide nutritional benefits to human health. Therefore, the assessment of the biological activity is considered as an important parameter for the promotion of high-quality food products. Herein, we introduce [...] Read more.
In recent years, there has been a strong consumer demand for food products that provide nutritional benefits to human health. Therefore, the assessment of the biological activity is considered as an important parameter for the promotion of high-quality food products. Herein, we introduce a novel methodology comprising a complete set of in vitro cell-free screening techniques for the evaluation of the bioactivity of various food products on the basis of their antioxidant capacity. These assays examine the free radical scavenging activities, the reducing properties, and the protective ability against oxidative damage to biomolecules. The adoption of the proposed battery of antioxidant assays is anticipated to contribute to the holistic characterization of the bioactivity of the food product under examination. Consumer motivations and expectations with respect to nutritious food products with bio-functional properties drive the global food market toward food certification. Therefore, the development and application of scientific methodologies that examine the quality characteristics of food products could increase consumers’ trust and promote their beneficial properties for human health. Full article
(This article belongs to the Special Issue Redox Biomarkers in the Evaluation of the Antioxidant Profile In Vitro and In Vivo)
12 pages, 667 KiB  
Systematic Review
School Performance among Children and Adolescents during COVID-19 Pandemic: A Systematic Review
by Eleni Panagouli, Androniki Stavridou, Christina Savvidi, Anastasia Kourti, Theodora Psaltopoulou, Theodoros N. Sergentanis and Artemis Tsitsika
Children 2021, 8(12), 1134; https://doi.org/10.3390/children8121134 - 4 Dec 2021
Cited by 77 | Viewed by 21246
Abstract
As a result of COVID-19 restrictions, conventional ways of schooling were not possible, and in order to continue the educational process new digital resources, such as online learning platforms, were imposed. Although virtual courses provided high-quality educational material, the efficiency in children’s and [...] Read more.
As a result of COVID-19 restrictions, conventional ways of schooling were not possible, and in order to continue the educational process new digital resources, such as online learning platforms, were imposed. Although virtual courses provided high-quality educational material, the efficiency in children’s and adolescents’ academic performance in general is yet to be known. The purpose of this systematic review is to examine whether the academic performance of school-aged students was impacted or not through online learning and modified educational methods during the ongoing COVID-19 pandemic. According to the studies, either students suffered from learning losses comparing to pre-pandemic years or, in some cases, they benefited from online learning, especially in mathematics. Younger students and students with neurodevelopmental disorders or special education needs seemed to suffer more. Parents/caregivers reported that their children’s performance deteriorated, while others thought that online learning was beneficial. Teachers also reported that students presented academic gaps and difficulties in mathematics and reading compared to typical years. Consequently, the new ways of schooling imposed by the restrictions have not been fully tested and the impact cannot be described thoroughly. The investment in technological equipment in schools for the majority of students, along with the training of teachers in digital competence, should be a priority. Full article
(This article belongs to the Special Issue New Frontiers in Adolescent Health and Development)
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18 pages, 299 KiB  
Review
Play Behaviors in Children during the COVID-19 Pandemic: A Review of the Literature
by Anastasia Kourti, Androniki Stavridou, Eleni Panagouli, Theodora Psaltopoulou, Maria Tsolia, Theodoros N. Sergentanis and Artemis Tsitsika
Children 2021, 8(8), 706; https://doi.org/10.3390/children8080706 - 17 Aug 2021
Cited by 41 | Viewed by 13571
Abstract
Play is a key factor for children’s healthy psychological, emotional, social, and cognitive development. During the COVID-19 pandemic, it has been postulated that children’s play was affected, not only regarding the time children spent playing but also in terms of the qualitative characteristics [...] Read more.
Play is a key factor for children’s healthy psychological, emotional, social, and cognitive development. During the COVID-19 pandemic, it has been postulated that children’s play was affected, not only regarding the time children spent playing but also in terms of the qualitative characteristics of play. The aim of this review was to investigate how children’s play has changed during the COVID-19 pandemic. A review was conducted in the PubMed, Google Scholar, EMBASE, SCOPUS, ERIC, PsycInfo, and JSTOR databases up to 6 December 2020. Furthermore, references of eligible studies as well as of relevant articles were searched using a snowballing technique. The search retrieved 17 eligible studies, conducted in Europe and North America. In general, outdoor play was reduced during the pandemic; on the other hand, there was an increase in indoor play and in videogames-screen time. COVID-19 was present in children’s pretend play. Children’s play was a key contributor to children’s mood and wellbeing. Furthermore, teachers were especially concerned about how children’s play was affected during the lockdown measures. There is evidence that children’s play habits were affected during the COVID-19 pandemic; further research is required, especially cross-culturally oriented. Full article
14 pages, 259 KiB  
Perspective
Sexual Activity in Adolescents and Young Adults through COVID-19 Pandemic
by Androniki Stavridou, Chrysa Samiakou, Anastasia Kourti, Stauroula Tsiorou, Eleni Panagouli, Athanasios Thirios, Theodora Psaltopoulou, Theodoros N. Sergentanis and Artemis Tsitsika
Children 2021, 8(7), 577; https://doi.org/10.3390/children8070577 - 5 Jul 2021
Cited by 21 | Viewed by 6636
Abstract
During the COVID-19 pandemic, it has been postulated that the sexual life of adolescent and young adults has been impacted in various aspects, potentially affecting their well-being. Our aim is to investigate the potential changes in the sexual activity and relationships of adolescents [...] Read more.
During the COVID-19 pandemic, it has been postulated that the sexual life of adolescent and young adults has been impacted in various aspects, potentially affecting their well-being. Our aim is to investigate the potential changes in the sexual activity and relationships of adolescents and young adults during the COVID-19 pandemic. In general, a decrease in sexual desire was reported during the COVID-19 pandemic, in both genders. Fewer sexual intercourses and bonding behaviors between partners were associated with loneliness and depressive symptoms. On the contrary, an increase in sexual desire was expressed in a few people, with masturbation to be the most preferable means of satisfaction. The present paper highlights the multifaceted impact of COVID-19 upon the sexual life of adolescents and young adults during the ongoing pandemic. The changes observed in their sexual activity and relationships, could provide the basis of future preventive and educational programs. Full article
26 pages, 1848 KiB  
Article
Supporting Europe’s Energy Policy Towards a Decarbonised Energy System: A Comparative Assessment
by Aikaterini Papapostolou, Charikleia Karakosta, Kalliopi-Anastasia Kourti, Haris Doukas and John Psarras
Sustainability 2019, 11(15), 4010; https://doi.org/10.3390/su11154010 - 24 Jul 2019
Cited by 18 | Viewed by 4114
Abstract
The European Union (EU) aims to prepare its strategy and infrastructure for further decarbonisation of its energy system in the longer term towards 2050. Recent political discussions and research interest focus on ways to accelerate the development and deployment of low-carbon technologies with [...] Read more.
The European Union (EU) aims to prepare its strategy and infrastructure for further decarbonisation of its energy system in the longer term towards 2050. Recent political discussions and research interest focus on ways to accelerate the development and deployment of low-carbon technologies with respect to the targets set for 2030 and 2050. However, the diverse options available that are to be implemented, are policy sensitive and need careful comparative assessment. This paper presents a multi-criteria approach based on an extension of the Preference Ranking Organization METHod for Enrichment of Evaluations (PROMETHEE) method for group decision-making that incorporates fuzzy set theory in order to evaluate alternative transformation pathways for achieving a sustainable energy system in EU. This assessment aims at providing a direction towards a most preferable pathway concept that should be taken into account by a future model-based analysis of the necessary transformation of our energy sector. The results obtained could support policymakers in drawing effective recommendations based on the findings. The added value of this analysis to policymakers is its contribution to plan climate and energy strategies towards a low-carbon transition pathway by using the information of this approach and prioritizing uncertainties through an environmental and energy perspective. Full article
(This article belongs to the Special Issue Sustainability as a Multi-criteria Concept: New Developments and Applications)
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