Relationship Between Obesity and Impairment of Cognitive Functions: An Investigation into the Integrated Role of Nutritional Education and Physical Activity in Lower Secondary School
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Participants
2.3. Procedures
2.4. Experimental Intervention
- Nutritional Education Intervention
- Physical Activity Intervention
Week | Nutritional Education (1 Weekly Session) | Physical Activity (2 Weekly Sessions) |
---|---|---|
1 | Introduction to the relationship between nutrition and the brain: how food influences mental performance | Basic body awareness: posture, breathing, relaxation |
2 | Macronutrients and cognitive functions: focus on carbohydrates, proteins, fats | Dynamic postural education and breath control |
3 | Glycemic index, attention and memory: effects of glycemic fluctuations | Balance exercises, motor control and spatiotemporal perception |
4 | Physiological vs. emotional hunger: self-regulation and bodily signals | Simple coordination paths and progressively challenging games |
5 | Nutrition labels: reading and critical interpretation | Rhythmic activities, attention and reaction games, motor memory tasks |
6 | Breakfast and school meals: impact on cognitive performance | Dual-task exercises: integration of motor tasks and cognitive challenges |
7 | Food and concentration: brain-friendly nutrients | Group motor paths with cooperative and decision-making tasks |
8 | Sugary drinks and attention decline | Outdoor activities: paced walking and environmental observation |
9 | Inflammation and obesity: effects on the brain and behavior | Spatial orientation games and environmental recognition |
10 | Building a balanced meal: nutritional logic and sustainability | Narrative motor activities in outdoor settings: movement and storytelling |
11 | Food stereotypes and social influences on eating choices | Multisensory motor experiences: bodily exploration of natural environments |
12 | Final reflection and self-assessment: toward conscious food choices | Integrative activities: review of motor and cognitive learning outcomes |
2.5. Measures
- Assessment of cognitive functions
- Digit Span Test—Forward and Backward (Wechsler Intelligence Scale for Children, WISC-IV)
- The Digit Span Test [27] was used to assess working memory and short-term attention. In the Forward version, the student must repeat a sequence of digits in the order presented, while in the Backward version, the student is required to repeat the digits in reverse order. Increasing the length of the sequence allows you to estimate the ability to actively maintain and manipulate information in memory. The Digit Span Test, both Forward and Backward, was used to assess working memory capacity. Previous research has reported acceptable internal consistency, with Cronbach’s alpha values ranging from 0.70 to 0.80 in school-aged children [28].
- Stroop Color and Word Test—Children’s Version
- The Stroop Color and Word Test [29] is a well-established tool for measuring inhibitory control and cognitive flexibility. Participants are asked to name the color of the ink while ignoring the semantic meaning of the written word. The test allows us to highlight the efficiency of the processes of automatic inhibition, switching and selective attention, considered sensitive markers of executive efficiency in developmental age. The Children’s Version of the Stroop Color and Word Test was employed to measure selective attention and cognitive inhibition. The test demonstrates satisfactory reliability, with Cronbach’s alpha values typically reported between 0.75 and 0.85 [30].
- Trail Making Test—Parts A and B (version adapted for school age)
- The Trail Making Test [31] assesses processing speed, the ability to toggle between different cognitive sets (letters and numbers), and the executive organization of behavior. The completion times of the two parts of the test, as well as the errors made, offer useful indicators of the level of cognitive flexibility and visuospatial planning. The adapted school-age version of the Trail Making Test (Parts A and B) was used to evaluate cognitive flexibility and processing speed. Test–retest reliability for children has been reported as good, with ICC values around 0.80 [32].
- Raven’s Progressive Matrices Test—Coloured Progressive Matrices (CPMs)
- Included as a measure of nonverbal logical reasoning and the ability to solve abstract visual problems, the Raven test [33] also allows us to detect any general differences in intellectual functioning between groups, controlling for any pre-existing cognitive biases. Raven’s Coloured Progressive Matrices (CPMs) were administered to assess non-verbal fluid intelligence. The internal consistency of the CPM is high, with Cronbach’s alpha typically above 0.85 [34].
- Assessment of motor and functional condition
- 6-Minute Walk Test (6MWT)
- The 6-Minute Walk Test [35] was used to estimate cardiorespiratory capacity and functional aerobic endurance. The test requires participants to cover the maximum possible distance in six minutes on a straight track. It was chosen for its validity in school contexts and sensitivity to changes related to regular physical activity. The 6-Minute Walk Test (6MWT) was used to evaluate submaximal aerobic capacity and functional endurance. Reliability studies in children report high test–retest reliability with ICC values ranging from 0.90 to 0.95 [36].
- Sit and Reach Test
- The Sit and Reach Test [37] measures the flexibility of the lower back and hamstrings. Although not directly related to cognitive functions, it represents a general index of muscle tone and joint mobility, influenced by the level of sedentary lifestyle. The Sit and Reach Test was administered to assess hamstring and lower back flexibility. It is a widely used field test with good test–retest reliability, with ICC values typically between 0.85 and 0.92 in children [38].
- Assessment of anthropometric parameters
- Body Mass Index (BMI)
- According to the criteria defined by Cole in 2002 [39], the body mass index (BMI) was calculated for each participant using the weight divided by height squared formula, expressed in kilograms per square meter (kg/m2), and interpreted with reference to internationally validated age- and sex-specific cut-offs for the pediatric population. This indicator was used both as a measure of weight status and as a continuous variable in the analysis of pre- and post-intervention variations, with the aim of detecting any changes in the body profile attributable to educational and motor interventions. Body Mass Index (BMI) was calculated using standardized procedures. Anthropometric measurements were conducted by trained personnel, and BMI demonstrates high inter-rater reliability, with ICC values exceeding 0.95 [40].
- Waist Circumference
- Abdominal circumference [41] was measured at the height of the navel with millimeter tape, as an additional indicator of metabolic risk and central fat accumulation, as it is more related to neuroinflammatory risks than BMI alone. Waist circumference was measured using a non-elastic tape at the midpoint between the last rib and the iliac crest. This measure has shown excellent inter-rater reliability in pediatric populations, with ICC values above 0.90 [42].
- Assessment of Nutritional Intervention
- Questionnaire on nutritional knowledge (adapted from Parmenter & Wardle, 1999 [43])
- We used a simplified version of this questionnaire adapted for school age [43], aimed at verifying the understanding of fundamental concepts related to food groups, energy requirements, meal balance and reading nutrition labels. It includes multiple choice and true/false questions, structured in thematic sections. The overall score makes it possible to quantify the effectiveness of the educational path in terms of knowledge acquisition. Nutritional knowledge was assessed using a questionnaire adapted from Parmenter and Wardle [43], which has demonstrated good internal consistency in various populations, with reported Cronbach’s alpha values around 0.78.
2.6. Statistical Analysis
3. Results
3.1. Confirmatory Factor Analysis and Construct Validity of the Measurement Scales
- Overall KMO = 0.81;
- Bartlett’s test: χ2(36) = 228.54, p < 0.001;
- χ2/df = 1.89;
- CFI = 0.95;
- TLI = 0.93;
- RMSEA = 0.064, 90% CI [0.048, 0.078];
- SRMR = 0.051.
3.2. Descriptive Statistics of Pre- and Post-Test Measurements
3.3. ANCOVA
3.4. Pearson Correlation
4. Discussion
5. Conclusions
6. Practical Implications
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | Experimental Group (n = 30) | Control Group (n = 30) | Total (N = 60) |
---|---|---|---|
Mean age (years) | 12.5 (±0.3) | 12.4 (±0.4) | 12.5 (±0.3) |
Gender (Female/Male) | 15/15 | 14/16 | 29/31 |
Mean BMI (kg/m2) | 23.4 (±2.8) | 23.1 (±2.6) | 23.3 (±2.7) |
Overweight percentage (%) | 40% | 37% | 38.5% |
Obesity percentage (%) | 30% | 27% | 28.5% |
Students with Law 104/92 certification | 1 | 2 | 3 |
Regular school attendance (%) | 100% | 100% | 100% |
Model Domain | χ2 (df) | χ2/df | CFI | TLI | RMSEA (90% CI) | SRMR | KMO | Bartlett’s Test (p) |
---|---|---|---|---|---|---|---|---|
Cognitive Function | 52.78 (28) | 1.88 | 0.94 | 0.92 | 0.065 [0.043–0.082] | 0.048 | 0.79 | <0.001 |
Physical and Anthropometric | 24.66 (14) | 1.76 | 0.96 | 0.94 | 0.059 [0.034–0.088] | 0.041 | 0.75 | <0.001 |
Nutritional Knowledge | 3.21 (2) | 1.60 | 0.99 | 0.98 | 0.042 [0.000–0.112] | 0.022 | 0.84 | <0.001 |
Overall 3-Factor Model | 84.65 (45) | 1.88 | 0.95 | 0.93 | 0.064 [0.048–0.078] | 0.051 | 0.81 | <0.001 |
Variable | Group | Pre-Test M | Pre-Test SD | Post-Test M | Post-Test SD | Δ (Post − Pre) M |
---|---|---|---|---|---|---|
Digit Span Forward | Experimental | 5.4 | 0.7 | 6.2 | 0.7 | +0.8 |
Control | 5.3 | 0.6 | 5.2 | 0.6 | −0.1 | |
Digit Span Backward | Experimental | 4.1 | 0.7 | 4.75 | 0.7 | +0.65 |
Control | 4.0 | 0.65 | 3.95 | 0.65 | −0.05 | |
Stroop Test (s) | Experimental | 55.4 | 5.8 | 49.2 | 5.5 | −6.2 |
Control | 54.9 | 6.0 | 54.8 | 6.1 | −0.1 | |
Trail Making Test B (s) | Experimental | 98.0 | 10.2 | 89.5 | 9.8 | −8.5 |
Control | 97.2 | 9.9 | 96.7 | 9.7 | −0.5 | |
6-Minute Walk Test (m) | Experimental | 590.0 | 35.0 | 632.4 | 34.8 | +42.4 |
Control | 588.5 | 33.5 | 598.7 | 32.8 | +10.2 | |
Sit and Reach Test (cm) | Experimental | 18.5 | 3.4 | 21.6 | 3.3 | +3.1 |
Control | 18.2 | 3.6 | 18.9 | 3.5 | +0.7 | |
Nutrition Knowledge Score | Experimental | 14.8 | 1.7 | 17.4 | 1.6 | +2.6 |
Control | 14.7 | 1.8 | 14.6 | 1.9 | −0.1 | |
BMI (kg/m2) | Experimental | 21.1 | 2.2 | 20.3 | 2.0 | −0.8 |
Control | 21.2 | 2.3 | 21.2 | 2.3 | 0.0 | |
Weight (kg) | Experimental | 49.4 | 5.15 | 47.5 | 4.68 | −1.9 |
Control | 49.6 | 5.38 | 49.6 | 5.38 | 0.0 | |
Waist Circumference (cm) | Experimental | 79.3 | 4.5 | 74.8 | 4.3 | −4.5 |
Control | 78.7 | 4.8 | 78.2 | 4.7 | −0.5 |
Variable | F(1,57) | p-Value | Partial η2 |
---|---|---|---|
Digit Span Forward | 14.86 | <0.001 | 0.21 |
Digit Span Backward | 12.45 | 0.001 | 0.18 |
Stroop Test (s) | 16.92 | <0.001 | 0.23 |
Trail Making Test B (s) | 11.08 | 0.001 | 0.16 |
6-Minute Walk Test (m) | 13.74 | <0.001 | 0.19 |
Sit and Reach Test (cm) | 10.33 | 0.002 | 0.15 |
Nutrition Knowledge Score | 18.60 | <0.001 | 0.25 |
BMI (kg/m2) | 6.40 | 0.05 | 0.10 |
Waist Circumference (cm) | 8.30 | 0.01 | 0.12 |
Weight (kg) | 7.10 | 0.01 | 0.11 |
Variable 1 | Variable 2 | Pearson’s r | p-Value |
---|---|---|---|
Digit Span Forward | 6-Minute Walk Test | 0.52 | 0.003 |
Digit Span Forward | Nutrition Knowledge Score | 0.49 | 0.005 |
Digit Span Backward | Sit and Reach Test | 0.46 | 0.008 |
Digit Span Backward | Nutrition Knowledge Score | 0.50 | 0.004 |
Trail Making Test B | 6-Minute Walk Test | −0.55 | 0.002 |
Trail Making Test B | Digit Span Backward | −0.48 | 0.006 |
Nutrition Knowledge | Trail Making Test B | −0.51 | 0.004 |
BMI (kg/m2) | 6-Minute Walk Test | −0.48 | 0.006 |
BMI (kg/m2) | Sit and Reach Test | −0.41 | 0.015 |
BMI (kg/m2) | Nutrition Knowledge Score | −0.37 | 0.028 |
Waist Circumference | 6-Minute Walk Test | −0.53 | 0.003 |
Waist Circumference | Sit and Reach Test | −0.45 | 0.009 |
Waist Circumference | Nutrition Knowledge Score | −0.39 | 0.022 |
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Tafuri, M.G.; Tafuri, D.; Latino, F. Relationship Between Obesity and Impairment of Cognitive Functions: An Investigation into the Integrated Role of Nutritional Education and Physical Activity in Lower Secondary School. Nutrients 2025, 17, 2531. https://doi.org/10.3390/nu17152531
Tafuri MG, Tafuri D, Latino F. Relationship Between Obesity and Impairment of Cognitive Functions: An Investigation into the Integrated Role of Nutritional Education and Physical Activity in Lower Secondary School. Nutrients. 2025; 17(15):2531. https://doi.org/10.3390/nu17152531
Chicago/Turabian StyleTafuri, Maria Giovanna, Domenico Tafuri, and Francesca Latino. 2025. "Relationship Between Obesity and Impairment of Cognitive Functions: An Investigation into the Integrated Role of Nutritional Education and Physical Activity in Lower Secondary School" Nutrients 17, no. 15: 2531. https://doi.org/10.3390/nu17152531
APA StyleTafuri, M. G., Tafuri, D., & Latino, F. (2025). Relationship Between Obesity and Impairment of Cognitive Functions: An Investigation into the Integrated Role of Nutritional Education and Physical Activity in Lower Secondary School. Nutrients, 17(15), 2531. https://doi.org/10.3390/nu17152531