Curcumin Ameliorates Nonalcoholic Fatty Liver Disease through Inhibition of O-GlcNAcylation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Immunoblot Analysis
2.3. Immunohistochemistry (IHC) and Oil Red O Staining
2.4. Quantitative Reverse Transcription–Polymerase Chain Reaction
2.5. Cell Culture and Transfection
2.6. The ROS Production Assay
2.7. Immunoprecipitation (IP)
2.8. Statistical Analysis
3. Results
3.1. Effect of Curcumin on Hepatic Steatosis in MCD Diet Mice
3.2. Curcumin Ameliorates Inflammation Induced in MCD Diet Mice
3.3. DE Analysis of Differentially Expressed Proteins
3.4. Curcumin Suppresses O-GlcNAcylation in Steatohepatitis
3.5. Regulation of Antioxidant Proteins in Palmitate-Induced AML12 Cells
3.6. Curcumin Ameliorates Inflammation by Inhibiting of O-GlcNAcylation and Activates Antioxidant Protein
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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No a | Identified Protein | Accession No. | MW (KDa) | PI | MOWSE Score | Coverage (%) | Fold Change | |
---|---|---|---|---|---|---|---|---|
MCD | MCD + Cur | |||||||
Metabolism | ||||||||
1 | 14-3-3 protein zeta/delta | P63101 | 27.8 | 4.7 | 30,049 | 44.9 | 4 | 3.2 |
2 | Annexin A5 | P48036 | 35.8 | 4.8 | 4.29 × 108 | 47.3 | 6 | 3.5 |
3 | 2’-5’-oligoadenylate synthase-like protein 1 | Q8VI94 | 59.1 | 6.7 | 139,169 | 22.9 | 3 | 1 |
Transport | ||||||||
4 | 40S ribosomal protein SA * | P14206 | 32.8 | 4.8 | 21,692 | 40.3 | 3 | 1 |
5 | Integrin beta-like protein 1 | Q8VDV0 | 54.0 | 5.3 | 14,545 | 20.0 | 2 | 0.8 |
Oxidative Stress/Detoxification processes | ||||||||
6/7 | AOP2(Peroxiredoxin-6) * | O08709 | 26.3 | 6.2 | 2350 | 18.5 | 0.4 | 1 |
8 | Glutathione peroxidase 1 (GPx 1) * | P11352 | 22.3 | 6.7 | 141,506 | 42.3 | 0.6 | 1.3 |
9 | SODC(Superoxide dismutase [Cu-Zn]) * | P08228 | 15.9 | 6.0 | 1996 | 41.6 | 0.3 | 0.8 |
10 | 60 kDa heat shock protein, mitochondrial * | P63038 | 61.0 | 5.9 | 1.60 × 106 | 43.8 | 2 | 1 |
Structural Proteins | ||||||||
11/12 | Keratin, type I cytoskeletal 18 * | P05784 | 47.5 | 5.2 | 3.72 × 107 | 36.6 | 5 | 1.5 |
13/14/15 | Keratin, type II cytoskeletal 8 * | P11679 | 54.6 | 5.7 | 1.25 ×107 | 42.0 | 3 | 1 |
16 | Actin-related protein T1 | Q9D9J3 | 42.2 | 5.2 | 701 | 18.6 | 5 | 3 |
17 | Rho GDP-dissociation inhibitor 1 * | Q99PT1 | 23.4 | 5.1 | 2545 | 41.7 | 4 | 2.5 |
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Lee, D.E.; Lee, S.J.; Kim, S.J.; Lee, H.-S.; Kwon, O.-S. Curcumin Ameliorates Nonalcoholic Fatty Liver Disease through Inhibition of O-GlcNAcylation. Nutrients 2019, 11, 2702. https://doi.org/10.3390/nu11112702
Lee DE, Lee SJ, Kim SJ, Lee H-S, Kwon O-S. Curcumin Ameliorates Nonalcoholic Fatty Liver Disease through Inhibition of O-GlcNAcylation. Nutrients. 2019; 11(11):2702. https://doi.org/10.3390/nu11112702
Chicago/Turabian StyleLee, Da Eun, Su Jin Lee, Su Ji Kim, Hyun-Shik Lee, and Oh-Shin Kwon. 2019. "Curcumin Ameliorates Nonalcoholic Fatty Liver Disease through Inhibition of O-GlcNAcylation" Nutrients 11, no. 11: 2702. https://doi.org/10.3390/nu11112702