An Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides Modifies the Fecal Microbiome and Metabolome in Infants with Cow’s Milk Protein Allergy
Abstract
:1. Introduction
2. Results
2.1. Cohort Description
2.2. Human Milk Oligosaccharides Supplementation Shifts Microbiome Development by Favoring a Bifidobacteria-Enriched Microbiome
2.3. Human Milk Oligosaccharides Supplementation Impacts Fecal Metabolites from Colonic Amino Acid and Bile Acid Metabolism in the EE Cohort
2.4. Fecal Ratios of Unconjugated/Conjugated Bile Acids and Acetic Acid Levels, Markers of Bifidobacterial Metabolism, Are Modulated by Human Milk Oligosaccharides in the EE Cohort
2.5. Omics Integration Describes Association between Bacterial Function and Fecal Metabolites Involved in Amino Acid and Bile Acids Metabolism
3. Discussion
4. Materials and Methods
4.1. Study Design and Participants
4.2. Interventions
4.3. Stool Collection
4.4. Fecal DNA Extraction and Ecological Measures
4.5. Fecal Community Type Clustering and Visualization
4.6. Statistical Analysis of Microbiome Data
4.7. Metabolomics of Fecal Samples and Statistical Analysis of Metabolomics Data
4.8. Metagenomics—Metabolomics Data Integration
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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PLS Model at V3 and V6 | WMW Test V3 | WMW Test V6 | ||||
---|---|---|---|---|---|---|
Metabolites | Correlation with Treatment | VIP Score | FC Test/Control | p Value | FC Test/Control | p Value |
2′-Fucosyllactose | 0.52 | 1.63 | 19.07 | 0.0001 | 2.44 | 0.0200 |
Lactose | 0.50 | 1.14 | 1.80 | 0.0033 | 0.98 | 0.5600 |
Hydroxybenzoic acid | 0.41 | 1.37 | 1.01 | 0.8100 | 2.34 | 0.0140 |
2-Hydroxyphenylacetic acid | −0.58 | 1.30 | 0.31 | 0.0100 | 0.60 | 0.054 |
3-Hydroxyphenylacetic acid | −0.28 | 1.52 | 0.24 | 0.0040 | 0.77 | 0.011 |
Phenylacetic acid | −0.70 | 1.35 | 0.24 | 0.0089 | 0.34 | 0.0065 |
Hydrocinnamic acid | −0.46 | 1.37 | 0.21 | 0.1400 | 0.25 | 0.0007 |
4-Cresol sulfate | −0.31 | 1.00 | 0.28 | 0.0320 | 0.40 | 0.14 |
L-Phenylalanine | −0.34 | 1.14 | 1.31 | 0.3700 | 0.41 | 0.0083 |
Isobutyric acid | −0.67 | 1.37 | 0.44 | 0.0098 | 0.36 | 0.0035 |
Isovaleric acid | −0.73 | 1.41 | 0.28 | 0.0080 | 0.44 | 0.00730 |
Pimelic acid | −0.22 | 1.43 | 0.58 | 0.1230 | 0.57 | 0.0210 |
Dehydrocholic acid | −0.30 | 1.62 | 0.51 | 0.0490 | 0.69 | 0.1800 |
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Boulangé, C.L.; Pedersen, H.K.; Martin, F.-P.; Siegwald, L.; Pallejà Caro, A.; Eklund, A.C.; Jia, W.; Zhang, H.; Berger, B.; Sprenger, N.; et al. An Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides Modifies the Fecal Microbiome and Metabolome in Infants with Cow’s Milk Protein Allergy. Int. J. Mol. Sci. 2023, 24, 11422. https://doi.org/10.3390/ijms241411422
Boulangé CL, Pedersen HK, Martin F-P, Siegwald L, Pallejà Caro A, Eklund AC, Jia W, Zhang H, Berger B, Sprenger N, et al. An Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides Modifies the Fecal Microbiome and Metabolome in Infants with Cow’s Milk Protein Allergy. International Journal of Molecular Sciences. 2023; 24(14):11422. https://doi.org/10.3390/ijms241411422
Chicago/Turabian StyleBoulangé, Claire L., Helle K. Pedersen, Francois-Pierre Martin, Léa Siegwald, Albert Pallejà Caro, Aron C. Eklund, Wei Jia, Huizhen Zhang, Bernard Berger, Norbert Sprenger, and et al. 2023. "An Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides Modifies the Fecal Microbiome and Metabolome in Infants with Cow’s Milk Protein Allergy" International Journal of Molecular Sciences 24, no. 14: 11422. https://doi.org/10.3390/ijms241411422