Aromadendrin Protects Neuronal Cells from Methamphetamine-Induced Neurotoxicity by Regulating Endoplasmic Reticulum Stress and PI3K/Akt/mTOR Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Aromadendrin Does Not Lead to Neuronal Toxicity in SH-SY5y Cells
2.2. Aromadendrin Suppresses METH-Induced Cell Death in SH-SY5y Cells
2.3. Aromadendrin Regulates METH-Induced ER Stress in SH-SY5y Cells
2.4. Aromadendrin Preserves the Phosphorylation of PI3K/Akt/mTOR Signaling Pathway in METH-Exposed SH-SY5y Cells
2.5. Aromadendrin Mitigates the METH-Induced Autophagy in Neuronal Cells
2.6. Pre-Treatment with Aromadendrin Has an Anti-Apoptotic Effect on METH-Exposed SH-SY5y Cells
2.7. Pre-Treatment with Aromadendrin Modulates the Expression of Apoptosis-Related Proteins in METH-Exposed Condition
2.8. Restored mTOR Phosphorylation by Aromadendrin Protects Cells from Autophagy and Apoptosis
3. Discussion
4. Materials and Methods
4.1. Cells
4.2. Isolation of Aromadendrin from C. retusus Flowers
4.3. Reagents and Antibodies
4.4. MTT Assay
4.5. Assessment of Cell Confluency
4.6. AnnexinV/PI Apoptosis Assay by Flow Cytometry
4.7. Real-Time Quantitative PCR
4.8. Western Blot Analysis
4.9. Determination of AnnexinV and Caspase3/7 Expression by IncuCyte® Imaging System
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
ARD | Aromadendrin |
METH | Methamphetamine |
mTOR | Mammalian target of rapamycin |
PI | Propidium iodide |
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Lee, H.-S.; Kim, E.-N.; Jeong, G.-S. Aromadendrin Protects Neuronal Cells from Methamphetamine-Induced Neurotoxicity by Regulating Endoplasmic Reticulum Stress and PI3K/Akt/mTOR Signaling Pathway. Int. J. Mol. Sci. 2021, 22, 2274. https://doi.org/10.3390/ijms22052274
Lee H-S, Kim E-N, Jeong G-S. Aromadendrin Protects Neuronal Cells from Methamphetamine-Induced Neurotoxicity by Regulating Endoplasmic Reticulum Stress and PI3K/Akt/mTOR Signaling Pathway. International Journal of Molecular Sciences. 2021; 22(5):2274. https://doi.org/10.3390/ijms22052274
Chicago/Turabian StyleLee, Hyun-Su, Eun-Nam Kim, and Gil-Saeng Jeong. 2021. "Aromadendrin Protects Neuronal Cells from Methamphetamine-Induced Neurotoxicity by Regulating Endoplasmic Reticulum Stress and PI3K/Akt/mTOR Signaling Pathway" International Journal of Molecular Sciences 22, no. 5: 2274. https://doi.org/10.3390/ijms22052274