DPP9 as a Potential Novel Mediator in Gastrointestinal Virus Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Colon Cohort
2.2. Jejunum Cohort
2.3. Metabolomics Validation Cohort
2.4. Clinical Biochemistry of Colon Cohort
2.5. Clinical Biochemistry of Jejunum Cohort
2.6. Colon Transcriptomics
2.7. Jejunum Transcriptomics
2.8. Circulating DPP9 Expression Analysis for Metabolomics Validation Cohort
2.9. Plasma Non-Targeted Metabolomics for Colon Cohort
2.10. Plasma Non-Targeted Metabolomics for Metabolomics Validation Cohort
2.11. Statistical Analysis
2.11.1. Gene Co-Expression Patterns
2.11.2. Enrichment Networks
2.11.3. Gene-Gene Interaction Networks
2.11.4. Machine Learning Variable Selection Analysis
2.11.5. Gene-Drug Enrichment Analyses
3. Results
3.1. The DPP9 Expression Levels in Jejunum, Colon, and Blood Are Strongly Associated with Biomarkers and Genes Involved in Oxidative Stress and Antioxidant Defense
3.2. Genes Associated to DPP9 Expression in the Colon Are Involved in Viral Replication, Antiviral Mechanisms and Metabolism of Nucleotides
3.3. Genes Associated to DPP9 Expression in the Jejunum Are Also Involved in Viral Replication and Antiviral Mechanisms
3.4. Metabolomics Signatures Associated with DPP9 in Plasma
3.5. Promising Drugs Targeting DPP9-Associated Genes
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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del Castillo-Izquierdo, Á.; Moreno-Navarrete, J.M.; Latorre, J.; Arnoriaga-Rodríguez, M.; Ballanti, M.; Monteleone, G.; Alessandro Paoluzi, O.; Mingrone, G.; Puig, J.; Ramos, R.; et al. DPP9 as a Potential Novel Mediator in Gastrointestinal Virus Infection. Antioxidants 2022, 11, 2177. https://doi.org/10.3390/antiox11112177
del Castillo-Izquierdo Á, Moreno-Navarrete JM, Latorre J, Arnoriaga-Rodríguez M, Ballanti M, Monteleone G, Alessandro Paoluzi O, Mingrone G, Puig J, Ramos R, et al. DPP9 as a Potential Novel Mediator in Gastrointestinal Virus Infection. Antioxidants. 2022; 11(11):2177. https://doi.org/10.3390/antiox11112177
Chicago/Turabian Styledel Castillo-Izquierdo, Ángela, José María Moreno-Navarrete, Jessica Latorre, María Arnoriaga-Rodríguez, Marta Ballanti, Giovanni Monteleone, Omero Alessandro Paoluzi, Geltrude Mingrone, Josep Puig, Rafael Ramos, and et al. 2022. "DPP9 as a Potential Novel Mediator in Gastrointestinal Virus Infection" Antioxidants 11, no. 11: 2177. https://doi.org/10.3390/antiox11112177