The Effects of Limonin, Myo-Inositol, and L-Proline on the Cryopreservation of Debao Boar Semen
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals Used for Semen Collection
2.2. Reagents and Extender
2.3. Semen Collection and Cryopreservation
2.4. Assessment of Frozen–Thawed Semen Motility, Morphology, and Kinematics
2.5. Plasma Membrane Integrity Assessment
2.6. Acrosome Integrity Assessment
2.7. Nuclear DNA Integrity Assessment
2.8. Measurement of Sperm Mitochondrial Activity
2.9. Detection of Antioxidant Capacity of Post-Thaw Sperm
2.10. Sperm Ultrastructure
2.11. In Vitro Fertilization
2.12. Experimental Design
2.13. Statistical Analysis
3. Results
3.1. Effects of Lim Supplementation on the Freezing Diluent on Boar Sperm Cryosurvival
3.2. Effects of MYO Supplementation on the Freezing Diluent on Boar Sperm Cryosurvival
3.3. Effects of LP Supplementation on the Freezing Diluent on Boar Sperm Cryosurvival
3.4. Effects of Combined Addition on the Freezing Diluent on Boar Sperm Cryosurvival
3.5. Effects of Different Concentrations of Glycerol on the Freezing Diluent on Boar Sperm Cryosurvival
3.6. Effects of Different Concentrations of Glycerol on Post-Thaw Boar Sperm Ultrastructure and Fertilization Ability
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Feng, Q.; Yang, Y.; Zhang, B.; Shi, W.; Fang, Y.; Xu, C.; Deng, Z.; Feng, W.; Shi, D. The Effects of Limonin, Myo-Inositol, and L-Proline on the Cryopreservation of Debao Boar Semen. Animals 2025, 15, 2204. https://doi.org/10.3390/ani15152204
Feng Q, Yang Y, Zhang B, Shi W, Fang Y, Xu C, Deng Z, Feng W, Shi D. The Effects of Limonin, Myo-Inositol, and L-Proline on the Cryopreservation of Debao Boar Semen. Animals. 2025; 15(15):2204. https://doi.org/10.3390/ani15152204
Chicago/Turabian StyleFeng, Qianhui, Yanyan Yang, Bing Zhang, Wen Shi, Yizhen Fang, Chunrong Xu, Zhuxin Deng, Wanyou Feng, and Deshun Shi. 2025. "The Effects of Limonin, Myo-Inositol, and L-Proline on the Cryopreservation of Debao Boar Semen" Animals 15, no. 15: 2204. https://doi.org/10.3390/ani15152204
APA StyleFeng, Q., Yang, Y., Zhang, B., Shi, W., Fang, Y., Xu, C., Deng, Z., Feng, W., & Shi, D. (2025). The Effects of Limonin, Myo-Inositol, and L-Proline on the Cryopreservation of Debao Boar Semen. Animals, 15(15), 2204. https://doi.org/10.3390/ani15152204