Isolation of Pseudomonas Strains with Potential for Protection of Soybean Plants against Saline Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Soil Sampling and Bacterial Isolation
2.2. Taxonomical Identification of the Isolates
2.3. Salinity Tolerance
2.4. Exopolysaccharide (EPS) Production, Bacterial Surface Adhesion, Colonization and Competition in Rhizosphere
2.5. Plant Growth Promotion in Slightly Saline Soil
2.6. Statistical Analysis
3. Results and Discussion
3.1. Soil Characteristics, Bacterial Isolation, and Selection of Isolates
3.2. Identification of Pseudomonas Isolates
3.3. Salt Tolerance Assays
3.4. EPS Production, Surface Adhesion, Colonization, and Competition in Rhizosphere
3.5. Plant Growth Promotion in Slightly Saline Soil
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Properties | Slightly Saline Soil | Strongly Saline Soil | Non-Saline Soil |
---|---|---|---|
Silt a (%) | 66.8 | 61.2 | 63.7 |
Clay a (%) | 5.7 | 14.5 | 5.0 |
Sand a (%) | 27.5 | 24.3 | 31.3 |
pH b | 8.4 | 8.8 | 8.1 |
EC c (dS/m) | 1.5 | 29.8 | 0.5 |
Sum of cations d (cmol/Kg) | 26.0 | 66.8 | 17.7 |
Na+ (ppm) | 498.9 | 6496.9 | 124.1 |
K+ (ppm) | 1049.1 | 1450.8 | 624.0 |
Ca++ (ppm) | 3834.0 | 1342.0 | 2750.0 |
Mg++ (ppm) | 230.4 | 50.4 | 219.6 |
Medium | Soybean Rhizosphere | Slightly Saline Soil Sample | Strongly Saline Soil Sample |
---|---|---|---|
LB 1× | 1.6 × 107 ± 5.0 × 106 a | 3.0 × 106 ± 3.0 × 105 b | 3.6 × 105 ± 8.0 × 104 b |
LB 1× + NaCl | 6.0 × 105 ± 4.0 × 104 a | 6.6 × 105 ± 1.2 × 105 a | 1.0 × 105 ± 1.5 × 104 b |
LB 0.1× | 1.8 ×107 ± 2.4 × 106 a | 4.2 × 106 ± 3.0 × 105 b | 5.7 × 105 ± 3.0 × 104 c |
LB 0.1× + NaCl | 3.0 × 105 ± 8.0 × 104 a | 4.5 × 105 ± 7.0 × 104 a | 1.4 × 105 ±1.2 × 104 b |
M9-glucose | 9.9 × 106 ± 7.0 × 105 a | 1.5 × 106 ± 3.1 × 105 b | 3.0 × 105 ± 4.0 × 104 b |
M9-glucose + NaCl | 5.4 × 105 ± 6.0 × 104 a | 4.2 × 105 ± 5.0 × 104 b | 4.5 × 104 ± 1.5 × 104 c |
M9-citrate | 9.0 × 106 ± 1.0 × 106 a | 1.4 × 106 ± 2.0 × 105 b | 1.8 × 105 ± 1.3 × 104 b |
M9-citrate + NaCl | 1.5 × 106 ± 2.0 × 105 a | 4.2 × 105 ± 7.0 × 104 b | 6.0 × 104 ± 4.0 × 103 c |
M9-benzoate | 6.5 × 105 ± 5.0 × 104 a | 3.9 × 105 ± 2.0 × 104 b | 4.5 × 105 ± 5.0 × 104 b |
M9-benzoate + NaCl | 6.0 × 106 ± 8.0 × 105 a | 4.8 × 105 ± 3.0 × 104 b | 2.0 × 103 ± 5.0 × 101 c |
Modified S1 | 1.2 × 106 ± 8.0 × 105 a | 6.0 × 103 ± 3.0 × 102 b | 4.2 × 102 ± 1.1 × 101 c |
Strains | Soybean Seeds (UFC/mL) | Soybean Roots in Sand + NaCl (CFU/g Root) | Soybean Roots in Slightly Saline Soil (CFU/g Root) |
---|---|---|---|
KT2440 | 1.6 106 ± 6.8 105 b | 3.6 106 ± 4.1 106 b | 2.4 106 ± 1.5 106 a |
EFY1 | 3.6 106 ± 2.6 106 b | 2.9 108 ± 3.8 108 a | 5.0 106 ± 7.6 105 a |
EFY2 | 6.4 106 ± 4.0 105 b | 8.9 108 ± 9.3 108 a | 3.8 106 ± 2.0 105 a |
EFY3 | 8.1 106 ± 2.8 106 a, b | 6.1 108 ± 1.0 108 a | 2.8 106 ± 4.3 106 a |
EFY4 | 1.6 107 ± 6.7 106 a | 7.9 108 ± 1.2 108 a | 7.1 106 ± 4.8 106 a |
Strains | Germination Rate (%) | Vigor Index | V2 (% of Plants in Phenological State V2) | |||
---|---|---|---|---|---|---|
Non-Saline | Saline | Non-Saline | Saline | Non-Saline | Saline | |
Control | 80.0 ± 5.6 c | 60.0 ± 5.6 c | 360.4 ± 82.9 c | 217.8 ± 38.2 c | 36.6 ± 4.7 b | 19.5 ± 3.0 c |
KT2440 | 96.0 ± 0.0 b | 94.0 ± 2.8 b | 438.0 ± 106.0 a,b | 344.9 ± 58.0 b | 47.9 ± 2.9a | 18.7 ± 2.9c |
EFY1 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 451,8 ± 77.9 a | 347.3 ± 72.5 b | 38.0 ± 2.8 b | 30.0 ± 2.8 b |
EFY2 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 388,2 ± 88.4 b,c | 356.5 ± 49.0 b | 14.0 ± 2.8 c | 10.0 ± 2.8 d |
EFY3 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 440,5 ± 100.4 a,b | 407.8 ± 68,0 a | 38.0 ± 2.8 b | 64.5 ± 2.9 a |
EFY4 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 408.9 ± 123.0 a,b,c | 396.2 ± 46.5 a | 34.0 ± 2.8 b | 31.2 ± 2.9 b |
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Costa-Gutierrez, S.B.; Caram-Di Santo, M.C.d.V.; Zenoff, A.M.; Espinosa-Urgel, M.; de Cristóbal, R.E.; Vincent, P.A. Isolation of Pseudomonas Strains with Potential for Protection of Soybean Plants against Saline Stress. Agronomy 2021, 11, 2236. https://doi.org/10.3390/agronomy11112236
Costa-Gutierrez SB, Caram-Di Santo MCdV, Zenoff AM, Espinosa-Urgel M, de Cristóbal RE, Vincent PA. Isolation of Pseudomonas Strains with Potential for Protection of Soybean Plants against Saline Stress. Agronomy. 2021; 11(11):2236. https://doi.org/10.3390/agronomy11112236
Chicago/Turabian StyleCosta-Gutierrez, Stefanie Bernardette, María Carolina del Valle Caram-Di Santo, Ana María Zenoff, Manuel Espinosa-Urgel, Ricardo Ezequiel de Cristóbal, and Paula Andrea Vincent. 2021. "Isolation of Pseudomonas Strains with Potential for Protection of Soybean Plants against Saline Stress" Agronomy 11, no. 11: 2236. https://doi.org/10.3390/agronomy11112236