Contraindicated Drug Responses in Dravet Syndrome Brain Organoids Utilizing Micro Electrode Array Assessment Methods
Abstract
:1. Introduction
2. Materials and Methods
2.1. DS Patient-Derived and Healthy Control Brain Organoids
2.2. Immunostaining
2.3. MEA Measurements
2.4. Pharmacological Testing
2.5. Measurement of DS Brain Organoid Using CMOS-MEA
2.6. Detection of Oscillations
2.7. Waveform Potential Analysis
2.8. Statistical Analysis
3. Results
3.1. MEA Measurments of Cerebral Brain Organoids Derived from iPSCs of DS Patients
3.2. Contraindicated Drug Responses in DS Brain Organoids
3.3. Carbamazepine Responses in DS Brain Organoids Using CMOS-MEA
3.4. Frequency Characteristics of DS Brain Organoids
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yokoi, R.; Nagafuku, N.; Ishibashi, Y.; Matsuda, N.; Suzuki, I. Contraindicated Drug Responses in Dravet Syndrome Brain Organoids Utilizing Micro Electrode Array Assessment Methods. Organoids 2023, 2, 177-191. https://doi.org/10.3390/organoids2040014
Yokoi R, Nagafuku N, Ishibashi Y, Matsuda N, Suzuki I. Contraindicated Drug Responses in Dravet Syndrome Brain Organoids Utilizing Micro Electrode Array Assessment Methods. Organoids. 2023; 2(4):177-191. https://doi.org/10.3390/organoids2040014
Chicago/Turabian StyleYokoi, Remi, Nami Nagafuku, Yuto Ishibashi, Naoki Matsuda, and Ikuro Suzuki. 2023. "Contraindicated Drug Responses in Dravet Syndrome Brain Organoids Utilizing Micro Electrode Array Assessment Methods" Organoids 2, no. 4: 177-191. https://doi.org/10.3390/organoids2040014