Hypolipidemic Properties of Cocoa and Coffee By-Products after Simulated Gastrointestinal Digestion: A Comparative Approach †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cocoa Shell and Coffee Pulp Flour and Aqueous Extracts Preparation
2.3. INFOGEST Static In Vitro Simulated Digestion
2.4. Cholesterol-Binding Capacity
2.5. Bile Salts-Binding Capacity
2.6. Inhibitory Activity against Pancreatic Lipase
2.7. Sample Preparation for HepG2 Cells
2.8. HepG2 Cell Cultures
2.8.1. Non-Alcoholic Fatty Liver Disease Induction in HepG2 Cells
2.8.2. Biological Hypolipidemic Activity
2.9. Statistical Analysis
3. Results and Discussion
3.1. Comparison of the In Vitro Hypolipidemic Properties of Cocoa Shell Flour and Coffee Pulp Flour Non-Digestible Fraction after In Vitro Static Digestion
3.2. Cocoa Shell and Coffee Pulp Were Not-Toxic and Regulated PA-Stimulated ROS Production and Lipid Accumulation
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- International Cocoa Organization Production of Cocoa Beans—ICCO Quarterly Bulletin of Cocoa Statics, Vol. XLVII (2020/21). Available online: https://www.icco.org/wp-content/uploads/Production_QBCS-XLVII-No.-1.pdf (accessed on 29 April 2021).
- International Coffee Organization World Coffee Production. 2021. Available online: https://www.ico.org/ (accessed on 29 April 2021).
- Iriondo-DeHond, A.; Aparicio García, N.; Fernandez-Gomez, B.; Guisantes-Batan, E.; Velázquez Escobar, F.; Blanch, G.P.; San Andres, M.I.; Sanchez-Fortun, S.; del Castillo, M.D. Validation of coffee by-products as novel food ingredients. Innov. Food Sci. Emerg. Technol. 2019, 51, 194–204. [Google Scholar] [CrossRef] [Green Version]
- Esquivel, P.; Jiménez, V.M. Functional properties of coffee and coffee by-products. Food Res. Int. 2012, 46, 488–495. [Google Scholar] [CrossRef]
- Rojo-Poveda, O.; Barbosa-Pereira, L.; Zeppa, G.; Stévigny, C. Cocoa bean shell—A by-product with nutritional properties and biofunctional potential. Nutrients 2020, 12, 1123. [Google Scholar] [CrossRef] [Green Version]
- Panak Balentić, J.; Ačkar, Đ.; Jokić, S.; Jozinović, A.; Babić, J.; Miličević, B.; Šubarić, D.; Pavlović, N. Cocoa Shell: A By-Product with Great Potential for Wide Application. Molecules 2018, 23, 1404. [Google Scholar] [CrossRef] [Green Version]
- Rebollo-Hernanz, M.; Cañas, S.; Aguilera, Y.; Benitez, V.; Gila-Díaz, A.; Rodriguez-Rodriguez, P.; Cobeta, I.M.; de Pablo, A.L.L.; Gonzalez, M.C.; Arribas, S.M.; et al. Validation of Cocoa Shell as a Novel Antioxidant Dietary Fiber Food Ingredient: Nutritional Value, Functional Properties, and Safety. Curr. Dev. Nutr. 2020, 4, 773. [Google Scholar] [CrossRef]
- Mussatto, S.I.; Machado, E.M.S.; Martins, S.; Teixeira, J.A. Production, Composition, and Application of Coffee and Its Industrial Residues. Food Bioprocess Technol. 2011, 4, 661–672. [Google Scholar] [CrossRef] [Green Version]
- Ulloa Rojas, J.B.; Verreth, J.A.J.; Amato, S.; Huisman, E.A. Biological treatments affect the chemical composition of coffee pulp. Bioresour. Technol. 2003, 89, 267–274. [Google Scholar] [CrossRef]
- Moreno, J.; Cozzano, S.; Mercedes Pérez, A.; Arcia, P.; Curutchet, A. Coffee Pulp Waste as a Functional Ingredient: Effect on Salty Cookies Quality. J. Food Nutr. Res. 2019, 7, 632–638. [Google Scholar] [CrossRef] [Green Version]
- Rebollo-Hernanz, M.; Zhang, Q.; Aguilera, Y.; Martín-Cabrejas, M.A.; de Mejia, E.G. Cocoa Shell Aqueous Phenolic Extract Preserves Mitochondrial Function and Insulin Sensitivity by Attenuating Inflammation between Macrophages and Adipocytes In Vitro. Mol. Nutr. Food Res. 2019, 63, 1801413. [Google Scholar] [CrossRef]
- Rebollo-Hernanz, M.; Willis, L.; Aguilera, Y.; Martin-Cabrejas, M.A.; Gonzalez de Mejia, E. Fibroblast Growth Factor 21 Signaling Activation by Selected Bioactive Compounds from Cocoa Shell Modulated Metabolism and Mitochondrial Function in Hepatocytes. Curr. Dev. Nutr. 2020, 4, 459. [Google Scholar] [CrossRef]
- Braojos, C.; Rebollo-Hernanz, M.; Benitez, V.; Cañas, S.; Aguilera, Y.; Arribas, S.; Martin-Cabrejas, M. Simulated gastrointestinal digestion influences the in vitro hypolipidemic properties of coffee pulp, a potential ingredient for the prevention of non-alcoholic fatty liver disease. Proceedings 2020, 61, 19. [Google Scholar] [CrossRef]
- Braojos, C.; Benitez, V.; Rebollo-Hernanz, M.; Cañas, S.; Aguilera, Y.; Arribas, S.M.; Martin-Cabrejas, M.A. Evaluation of the Hypolipidemic Properties of Cocoa Shell after Simulated Digestion Using In Vitro Techniques and a Cell Culture Model of Non-Alcoholic Fatty Liver Disease. Proceedings 2020, 70, 58. [Google Scholar] [CrossRef]
- Rebollo-Hernanz, M.; Cañas, S.; Taladrid, D.; Bartolomé, B.; Aguilera, Y.; Martin-Cabrejas, M.A. Extraction of phenolic compounds from cocoa shell: Modeling using response surface methodology and artificial neural networks. Sep. Purif. Technol. 2021, 270, 118779. [Google Scholar] [CrossRef]
- Rebollo-Hernanz, M.; Cañas, S.; Taladrid, D.; Benítez, V.; Bartolomé, B.; Aguilera, Y.; Martín-Cabrejas, M.A.; Rebollo-Hernanz, M.; Cañas, S.; Taladrid, D.; et al. Revalorization of coffee husk: Modeling and optimizing the green-sustainable extraction of phenolic compounds. Foods 2021, 10, 653. [Google Scholar] [CrossRef] [PubMed]
- Brodkorb, A.; Egger, L.; Alminger, M.; Alvito, P.; Assunção, R.; Ballance, S.; Bohn, T.; Bourlieu-Lacanal, C.; Boutrou, R.; Carrière, F.; et al. INFOGEST static in vitro simulation of gastrointestinal food digestion. Nat. Protoc. 2019, 14, 991–1014. [Google Scholar] [CrossRef] [PubMed]
- Papillo, V.A.; Vitaglione, P.; Graziani, G.; Gokmen, V.; Fogliano, V. Release of antioxidant capacity from five plant foods during a multistep enzymatic digestion protocol. J. Agric. Food Chem. 2014, 62, 4119–4126. [Google Scholar] [CrossRef]
- Benitez, V.; Rebollo-Hernanz, M.; Hernanz, S.; Chantres, S.; Aguilera, Y.; Martin-Cabrejas, M.A. Coffee parchment as a new dietary fiber ingredient: Functional and physiological characterization. Food Res. Int. 2019, 122, 105–113. [Google Scholar] [CrossRef]
- Benítez, V.; Rebollo-Hernanz, M.; Aguilera, Y.; Bejerano, S.; Cañas, S.; Martín-Cabrejas, M.A. Extruded coffee parchment shows enhanced antioxidant, hypoglycaemic, and hypolipidemic properties by releasing phenolic compounds from the fibre matrix. Food Funct. 2021, 12, 1097. [Google Scholar] [CrossRef]
- Rebollo-Hernanz, M.; Zhang, Q.; Aguilera, Y.; Martín-Cabrejas, M.A.; Gonzalez de Mejia, E. Phenolic compounds from coffee by-products modulate adipogenesis-related inflammation, mitochondrial dysfunction, and insulin resistance in adipocytes, via insulin/PI3K/AKT signaling pathways. Food Chem. Toxicol. 2019, 132, 110672. [Google Scholar] [CrossRef]
- Capuano, E. The behavior of dietary fiber in the gastrointestinal tract determines its physiological effect. Crit. Rev. Food Sci. Nutr. 2017, 57, 3543–3564. [Google Scholar] [CrossRef] [Green Version]
- Story, J.A.; Kritchevsky, D. Comparison of the Binding of Various Bile Acids and Bile Salts in Vitro by Several Types of Fiber. J. Nutr. 1976, 9, 1292–1294. [Google Scholar] [CrossRef]
- Donnelly, K.L.; Smith, C.I.; Schwarzenberg, S.J.; Jessurun, J.; Boldt, M.D.; Parks, E.J. Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. J. Clin. Investig. 2005, 115, 1343–1351. [Google Scholar] [CrossRef] [Green Version]
- Beysen, C.; Schroeder, P.; Wu, E.; Brevard, J.; Ribadeneira, M.; Lu, W.; Dole, K.; O’Reilly, T.; Morrow, L.; Hompesch, M.; et al. Inhibition of fatty acid synthase with FT-4101 safely reduces hepatic de novo lipogenesis and steatosis in obese subjects with non-alcoholic fatty liver disease: Results from two early-phase randomized trials. Diabetes Obes. Metab. 2021, 23, 700–710. [Google Scholar] [CrossRef]
- Baeza, G.; Amigo-Benavent, M.; Sarriá, B.; Goya, L.; Mateos, R.; Bravo, L. Green coffee hydroxycinnamic acids but not caffeine protect human HepG2 cells against oxidative stress. Food Res. Int. 2014, 62, 1038–1046. [Google Scholar] [CrossRef] [Green Version]
- Rebollo-Hernanz, M.; Zhang, Q.; Aguilera, Y.; Martín-Cabrejas, M.A.; Gonzalez de Mejia, E. Relationship of the phytochemicals from coffee and cocoa by-products with their potential to modulate biomarkers of metabolic syndrome in vitro. Antioxidants 2019, 8, 279. [Google Scholar] [CrossRef] [Green Version]
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Braojos, C.; Rebollo-Hernanz, M.; Cañas, S.; Aguilera, Y.; Arribas, S.M.; Martín-Cabrejas, M.A.; Benítez, V. Hypolipidemic Properties of Cocoa and Coffee By-Products after Simulated Gastrointestinal Digestion: A Comparative Approach. Biol. Life Sci. Forum 2021, 7, 1. https://doi.org/10.3390/ECB2021-10288
Braojos C, Rebollo-Hernanz M, Cañas S, Aguilera Y, Arribas SM, Martín-Cabrejas MA, Benítez V. Hypolipidemic Properties of Cocoa and Coffee By-Products after Simulated Gastrointestinal Digestion: A Comparative Approach. Biology and Life Sciences Forum. 2021; 7(1):1. https://doi.org/10.3390/ECB2021-10288
Chicago/Turabian StyleBraojos, Cheyenne, Miguel Rebollo-Hernanz, Silvia Cañas, Yolanda Aguilera, Silvia M. Arribas, María. A. Martín-Cabrejas, and Vanesa Benítez. 2021. "Hypolipidemic Properties of Cocoa and Coffee By-Products after Simulated Gastrointestinal Digestion: A Comparative Approach" Biology and Life Sciences Forum 7, no. 1: 1. https://doi.org/10.3390/ECB2021-10288
APA StyleBraojos, C., Rebollo-Hernanz, M., Cañas, S., Aguilera, Y., Arribas, S. M., Martín-Cabrejas, M. A., & Benítez, V. (2021). Hypolipidemic Properties of Cocoa and Coffee By-Products after Simulated Gastrointestinal Digestion: A Comparative Approach. Biology and Life Sciences Forum, 7(1), 1. https://doi.org/10.3390/ECB2021-10288