Next Article in Journal
Beneficial Effects of Ketogenic Diet on Nonalcoholic Steatohepatitis in Obese Mice Model
Previous Article in Journal
Identifying Environmental Refuges (“Coldspots”) from Infection by Batrachochytrium Dendrobatidis of Amphibians in Eastern Europe
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:

Characterization of Polyphenolic Composition of Extracts from Winery Wastes by HPLC-UV-MS/MS †

Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain
Research Institute in Food Nutrition and Food Safety, University of Barcelona, Av. Prat de la Riba 171, Edifici Recerca (Gaudí), E08921 Santa Coloma de Gramenet, Spain
Generalitat de Catalunya, Rambla de Catalunya 19-21, E08007 Barcelona, Spain
Author to whom correspondence should be addressed.
Presented at the 2nd International Electronic Conference on Foods—Future Foods and Food Technologies for a Sustainable World, 15–30 October 2021; Available online:
Biol. Life Sci. Forum 2021, 6(1), 78;
Published: 13 October 2021


Winery wastes are rich in polyphenols and have great added value when used in cosmetics, pharmaceuticals and agri-food products. Polyphenols display a wide range of positive health effects, thus helping with the prevention or treatment of some diseases because of their great antioxidant, anti-inflammatory, antimicrobial and antineoplastic properties. This work aims at characterizing the polyphenols recovered from various wastes generated during wine production processes using chromatographic techniques. Phenolic acids, flavonoids and related compounds have been extracted from different types of oenological residues, such as lees from malolactic fermentation and husks, by liquid extraction using aqueous or hydro-organic solvents. The resulting extracts have been further analyzed by liquid chromatography with UV/vis and mass spectrometric (MS) detection. The chromatographic separation was carried out by a reversed-phase mode on a core-shell C18 column using 0.1% formic acid in water and acetonitrile as the components of the mobile phase. Chromatograms were acquired with UV detection at 250, 280, 325 and 370 nm, whereas MS detection, with both low- and high-resolution analyzers, was performed using different acquisition modes, including full scan, data-dependent acquisition (DDA) and multiple reaction monitoring (MRM). The most abundant polyphenols in the extracts were identified as follows: caftaric acid with a concentration of 20 mg L−1, and trans-coutaric acid, cis-coutaric acid, caffeic acid and p-coumaric acid with concentrations of 5 mg L−1 or less. Other minor polyphenols such as gallic acid, 3,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, syringic acid, chlorogenic acid, ethyl gallate, ferulic acid, catechin, epicatechin, rutin, astilbin and resveratrol were also found.

Supplementary Materials

The following supporting information can be downloaded at:, Poster: Characterization of polyphenolic composition of extracts from winery wastes by HPLC-UV-MS/MS.

Author Contributions

Conceptualization, M.G. and J.S.; methodology, I.C.; investigation, I.C.; data curation, I.C. and S.S.; writing—original draft preparation, M.G. and S.S.; writing—review and editing, M.G., J.S. and S.S.; supervision, M.G., J.S. and S.S. All authors have read and agreed to the published version of the manuscript.


This research has been funded by the “Agencia Estatal de Investigación” of the Spanish Ministry of Science and Innovation, grant number PID2020-114401RB-C22.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

Data is available upon request to the authors.


This research was supported by the R2MIT (CTM2017-85346-R) project financed by the Spanish Ministry of Economy and Competitiveness, the W4V (PID2020-114401RB-C22) project financed by the Spanish Ministry of Science and Innovation, and the Catalan Government (2017-SGR-312).

Conflicts of Interest

The authors declare no conflict of interest.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Carretero, I.; Granados, M.; Saurina, J.; Sentellas, S. Characterization of Polyphenolic Composition of Extracts from Winery Wastes by HPLC-UV-MS/MS. Biol. Life Sci. Forum 2021, 6, 78.

AMA Style

Carretero I, Granados M, Saurina J, Sentellas S. Characterization of Polyphenolic Composition of Extracts from Winery Wastes by HPLC-UV-MS/MS. Biology and Life Sciences Forum. 2021; 6(1):78.

Chicago/Turabian Style

Carretero, Iris, Mercè Granados, Javier Saurina, and Sònia Sentellas. 2021. "Characterization of Polyphenolic Composition of Extracts from Winery Wastes by HPLC-UV-MS/MS" Biology and Life Sciences Forum 6, no. 1: 78.

Article Metrics

Back to TopTop