Abstract
There is a close correlation between type 2 diabetes mellitus (T2DM) and cognitive impairment leading to dementia. Lately, the incidence of T2DM-related dementia has increased with population aging. Factors such as oxidative stress and inflammatory responses may contribute to brain dysfunction in diabetes. The major inflammatory response found in diabetic rat brains occurs through the activation of nuclear factor-kappa B (NF-κB), and consequently, the expression of pro-inflammatory cytokines. Silicon is a micronutrient with antidiabetic, antioxidant, and anti-inflammatory properties; however, its effects on the inflammatory responses in the brain of T2DM rats are unclear. This study aimed to evaluate the anti-inflammatory effect of silicon in the cerebral cortex and hippocampus of late-stage T2DM rats. A late-stage diabetic model was established by injection of a low-dose streptozotocin plus nicotinamide combined with following the experimental diets. Sixteen rats were divided into two groups. The diabetic group (D) was fed a saturated-fat hypercholesterolemic diet containing a control restructured meat matrix (RM). In the treatment group, silicon was included into RM as a functional ingredient (D-Si). The NF-kB, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels were measured by immunohistochemistry in the cortex and hippocampus. Silicon down-regulated NF-κB activation, showing lower pNF-κB-labeled cells and lower immunoreactivity in both the cortex and hippocampus (p < 0.05). TNF-α levels decreased in both brain areas of D-Si rats (p < 0.05); whereas IL-6 levels were only decreased in cortex (p < 0.05). These results showed that silicon decreased the NF-κB pro-inflammatory pathway in cortex and hippocampus of late-stage T2DM rats. However, a further comprehension of underlying mechanisms would be interesting. It can be concluded that silicon administration as a functional ingredient may offer a novel nutritional strategy in the neuroprotection of T2DM-associated cognitive impairment.
Author Contributions
Conceptualization, M.E.L.-O., A.G., S.B., F.J.S.-M. and J.B.; methodology, R.R.-C., M.H.-M. and A.M.-G.; software, R.R.-C.; validation, M.E.L.-O., A.G., A.B. and J.B., formal analysis, R.R.-C., M.E.L.-O. and A.G.; investigation, R.R.-C., M.H.-M. and A.M.-G.; resources, M.J.G.-M.; data curation, R.R.-C., M.E.L.-O. and A.G.; writing—original draft preparation, R.R.-C., M.E.L.-O. and A.G.; writing—review and editing, R.R.-C., A.G. M.E.L.-O. and A.B.; visualization, R.R.-C., A.G., M.E.L.-O. and A.B.; supervision, A.G., M.E.L.-O. and J.B.; project administration, J.B. and F.J.S.-M.; funding acquisition, M.E.L.-O., A.G., S.B., M.J.G.-M., J.B. and F.J.S.-M. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by Spanish project PID2019-103872RB-I00. R.R.-C. was supported by grant FPU20/02920 from the Spanish Ministry of Universities.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Conflicts of Interest
The authors declare no conflict of interest.
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