Prolonged Cold Ischemia Did Not Impair Mitochondrial Oxygen Consumption or Reactive Oxygen Species Production in Human Uterine Fundus and Horn Myometrium
Abstract
:1. Introduction
2. Population and Methods
2.1. Study Design
2.2. Parameters Determined
2.2.1. Mitochondrial Use of Oxygen
2.2.2. Reactive Oxygen Species Production
2.2.3. Free Radical Leak
2.3. Statistical Analysis
3. Results
3.1. Clinical Characteristics of the Patients
3.2. Mitochondrial Oxygen Consumption in Uterine Fundus and Horn after Two, Seven, and Twenty-Four Hours of Cold Ischemia
3.2.1. Uterine Fundus
3.2.2. Uterine Horn
3.3. ROS Production at the Uterine Fundus and Horn after Two, Seven, and Twenty-Four Hours of Cold Ischemia
3.3.1. Uterine Fundus
3.3.2. Uterine Horn
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Clinical Data | Mean ± SD, Min–Max |
---|---|
Age (mean ± SD, min–max) | 47.6 ± 3.9 (44–57) |
Gravidity (mean ± SD, min–max) * | 2.37 ± 1.49 (0–5) |
Parity (mean ± SD, min–max) ** | 2 ± 1.1 (0–4) |
Smoker (n/8) | 1 |
Post–menopausal (n/8) | 0 |
Cardiovascular disease (n/8) | 2 |
Indication (n/8) | 4 myomas–3 adenomyosis–1 prolapse |
Type of hysterectomy (n/8) | 5 laparoscopic–3 vaginal–0 laparotomy |
Histology(n/8) | 2 myomas–3 adenomyosis 1 sarcoma–1 normal tissue |
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Pélissié, M.; Charles, A.-L.; Goupilleau, F.; Georg, I.; Bryand, A.; Geny, B.; Garbin, O. Prolonged Cold Ischemia Did Not Impair Mitochondrial Oxygen Consumption or Reactive Oxygen Species Production in Human Uterine Fundus and Horn Myometrium. Oxygen 2022, 2, 12-21. https://doi.org/10.3390/oxygen2010002
Pélissié M, Charles A-L, Goupilleau F, Georg I, Bryand A, Geny B, Garbin O. Prolonged Cold Ischemia Did Not Impair Mitochondrial Oxygen Consumption or Reactive Oxygen Species Production in Human Uterine Fundus and Horn Myometrium. Oxygen. 2022; 2(1):12-21. https://doi.org/10.3390/oxygen2010002
Chicago/Turabian StylePélissié, Mathilde, Anne-Laure Charles, Fabienne Goupilleau, Isabelle Georg, Angélique Bryand, Bernard Geny, and Olivier Garbin. 2022. "Prolonged Cold Ischemia Did Not Impair Mitochondrial Oxygen Consumption or Reactive Oxygen Species Production in Human Uterine Fundus and Horn Myometrium" Oxygen 2, no. 1: 12-21. https://doi.org/10.3390/oxygen2010002