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Article

A Thorough Insight into the Biofilm Properties of Potential Fish Pathogen Tenacibaculum discolor Strain FMCC B487

by
Eirini Schoina
1,
Laetitia Marchand
2,
Agata Zykwinska
2,
Corinne Sinquin
2,
Françoise Leroi
2,
Christine Delbarre-Ladrat
2 and
George-John E. Nychas
1,*
1
Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, 11855 Athens, Greece
2
Ifremer, MASAE Microbiologie Aliment Santé Environment, F-44000 Nantes, France
*
Author to whom correspondence should be addressed.
Aquac. J. 2024, 4(2), 76-91; https://doi.org/10.3390/aquacj4020006
Submission received: 4 April 2024 / Revised: 1 June 2024 / Accepted: 7 June 2024 / Published: 11 June 2024

Abstract

Tenacibaculum discolor develops biofilm in marine aquaculture production tanks and is identified as one of the causative agents of tenacibaculosis, a bacterial disease that causes significant losses in marine aquaculture production. In this study, the biofilm characteristics of T. discolor strain FMCC B487 were evaluated. Cell growth and biofilm formation and development were studied in miniaturized assays to assess the effect of different levels of environmental factors temperature and salinity, as well as the presence of monosaccharides potentially found in aquaculture hatcheries. The ability of the strain to grow and develop strong biofilms in ambient to high temperatures and at salinities above 20 g/L was shown. Mannose was the monosaccharide with the most prominent impact on the T. discolor strain FMCC B487 biofilm. The composition of planktonic cell extract, biofilm extracts, and extracellular polymeric substances (EPS) produced by T. discolor strain FMCC B487 were investigated by means of colorimetric and fluorometric assays as well as analyses by electrophoresis, gas chromatography, and high-performance size-exclusion chromatography coupled with a multiangle light scattering detector, revealing the dominance of proteins and lipids and the absence of high-molecular-weight polysaccharides. This information may serve as a basis for considering anti-biofilm strategies against the pathogen T. discolor.
Keywords: Tenacibaculum; salinity; temperature; biofilm; proteins; extracellular polymeric substances; mannose Tenacibaculum; salinity; temperature; biofilm; proteins; extracellular polymeric substances; mannose

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MDPI and ACS Style

Schoina, E.; Marchand, L.; Zykwinska, A.; Sinquin, C.; Leroi, F.; Delbarre-Ladrat, C.; Nychas, G.-J.E. A Thorough Insight into the Biofilm Properties of Potential Fish Pathogen Tenacibaculum discolor Strain FMCC B487. Aquac. J. 2024, 4, 76-91. https://doi.org/10.3390/aquacj4020006

AMA Style

Schoina E, Marchand L, Zykwinska A, Sinquin C, Leroi F, Delbarre-Ladrat C, Nychas G-JE. A Thorough Insight into the Biofilm Properties of Potential Fish Pathogen Tenacibaculum discolor Strain FMCC B487. Aquaculture Journal. 2024; 4(2):76-91. https://doi.org/10.3390/aquacj4020006

Chicago/Turabian Style

Schoina, Eirini, Laetitia Marchand, Agata Zykwinska, Corinne Sinquin, Françoise Leroi, Christine Delbarre-Ladrat, and George-John E. Nychas. 2024. "A Thorough Insight into the Biofilm Properties of Potential Fish Pathogen Tenacibaculum discolor Strain FMCC B487" Aquaculture Journal 4, no. 2: 76-91. https://doi.org/10.3390/aquacj4020006

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