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Article

Correlation of SERPINA-1 Gene Over-Expression with Inhibition of Cell Proliferation and Modulation of the Expression of IL-6, Furin, and NSD2 Genes

by
Nassim Tassou
1,2,*,
Hajar Anibat
1,2,
Ahmed Tissent
1 and
Norddine Habti
1,2,*
1
Laboratory of Hematology, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca 20100, Morocco
2
Laboratory of Cellular and Molecular Inflammatory, Degenerative, and Oncological Pathophysiology, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca 20250, Morocco
*
Authors to whom correspondence should be addressed.
Biologics 2025, 5(3), 22; https://doi.org/10.3390/biologics5030022
Submission received: 7 June 2025 / Revised: 3 July 2025 / Accepted: 29 July 2025 / Published: 6 August 2025
(This article belongs to the Topic Advances in Anti-Cancer Drugs: 2nd Edition)
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Abstract

Background and Objectives: The cytokine IL-6, methyltransferase NSD2, pro-protein convertase Furin, and growth factor receptor IGF-1R are essential factors in the proliferation of cancer cells. These proteins are involved in the tumor process by generating several cell-signaling pathways. However, the interactions of these oncogenic biomarkers, Furin, IL-6, and NSD2, and their links with the inhibitor SERPINA-1 remain largely unknown. Materials and Methods: Cell proliferation is measured by colorimetric and enzymatic methods. The genetic expressions of SERPINA-1, Furin, IL-6, and NSD2 are measured by qRT-PCR, while the expression of IGF-1R on the cell surface is measured by flow cytometry. Results: The proliferation of cells overexpressing SERPINA-1 (JP7pSer+) is decreased by more than 90% compared to control cells (JP7pSer-). The kinetics of the gene expression ratios of Furin, IL-6, and NSD2 show an increase for 48 h, followed by a decrease after 72 h for the three biomarkers in JP7pSer+ cells compared to JP7pSer- cells. The expression of IGF-1R on the cell surface in both cell lines is low, with JP7pSer- cells expressing 1.33 times more IGF-1R than JP7pSer+ cells. Conclusions: These results suggest gene correlations of SERPINA-1 overexpression with decreased cell proliferation and modulation of gene expression of Furin, IL-6, and NSD2. This study should be complemented by molecular transcriptomic and proteomic experiments to better understand the interaction of SERPINA-1 with IL-6, Furin, and NSD2, and their effect on tumor progression.

1. Introduction

Cancer is a complex disease characterized by uncontrolled cell proliferation, driven by a combination of genetic, environmental, and epigenetic factors [1]. The cellular signaling pathways are central to these events, which regulate cell growth, survival, and differentiation [2,3]. These pathways are complex networks of proteins involved in genetic and epigenetic regulation, as well as in modulating the cellular environment, influencing fundamental processes such as apoptosis, cell proliferation, and tissue invasion [3,4,5]. Prominent signaling pathways frequently deregulated in cancer development include the PI3K/AKT/mTOR, MAPK/ERK, and JAK/STAT pathways [6,7,8,9].
These pathways are often abnormally activated by genetic mutations or aberrant activation mechanisms, leading to excessive stimulation of proliferation and inhibition of apoptosis mechanisms [10,11,12]. Indeed, the JAK/STAT pathway, when activated by IL-6, a cytokine implicated in several cancers [9], induces the activation of the transcription factor STAT3 in tumor cells. The activation of the NF-κB and STAT3 signaling pathways promotes resistance to apoptosis, angiogenesis, and metastasis of tumor cells [13,14]. Furthermore, IL-6 contributes to the resistance of tumor cells to treatment, such as chemotherapy, by promoting survival and invasion and by inhibiting apoptosis of malignant cells [9,15].
IL-6 plays a role in modulating the tumor microenvironment, promoting inflammation and tumor growth, such as in multiple myeloma [16,17]. Song and colleagues demonstrated that NSD2 can methylate and activate STAT3, creating a potential positive feedback loop that amplifies IL-6 signaling [18]. NSD2 is a methyltransferase that plays a key role in the regulation of gene expression by methylating histones. This enzyme is responsible for the catalysis of mono- and di-methylation of lysine 36 on histone H3 [19]. This histone modification can recruit or exclude other chromatin-associated proteins, leading to either activation or repression of gene expression, depending on the cellular context [20]. NSD2 expression is frequently elevated in various types of cancers [18,19,20,21]. The evolution of cancer involves complex biological events that depend on the activity of various enzymes. Among these, proprotein convertases, particularly Furin, are involved in the maturation of numerous precursor proteins into their active forms, thereby promoting tumor progression [22,23]. Furin, a subtilisin-like protease, cleaves precursor proteins at the consensus site of Arg—X—Lys/Arg—Arg or Arg—X—X—Arg, facilitating their maturation [24,25,26].
Furin is involved in the maturation of precursors such as pro-TNF-α, pro-TGF-β1, and pro-IGF-1R, as well as various growth factors. These molecules play important roles in diverse cellular processes, including inflammation, growth, and angiogenesis, which are frequently deregulated in malignant cells [26,27,28]. In the context of the tumor microenvironment, pro-inflammatory cytokines matured by Furin, such as pro-TNF-α and pro-TGF-β1, can induce the activation of IL-6 gene expression [29], which in turn promotes tumor growth, angiogenesis, and metastasis [13]. Indeed, IL-6 produced by tumor microenvironment cells induces the activation of the JAK/STAT3 pathway in tumor cells, stimulating their proliferation, survival, and invasion [9]. Furin, therefore, contributes to the regulation of several signaling pathways crucial for tumor progression, including the maturation of the pro-IGF-1R precursor into IGF-1R, enabling its expression on the cell surface [30,31]. By interacting with its ligands IGF1 and IGF2, IGF-1R activates cell proliferation and promotes tumor progression [32]. Furin is often overexpressed in various carcinomas, suggesting its potential role in the survival and proliferation of tumor cells [26].
However, the involvement of Furin in different cancers, such as malignant hematological diseases, remains largely unknown, particularly regarding its potential interactions with IL-6 and NSD2. Studies on the links between these markers would help identify common mechanistic pathways and specificities in different cancer pathologies. To date, no study has established a definitive mechanistic link between these three players (Furin, IL-6, and NSD2) in cancer, which limits the understanding of their synergies or common regulatory pathways. In a proliferative hetero-hybrid lymphoblastoid cell model, an in vitro approach involves verifying whether there are significant correlations between the expression of Furin, IL-6, and NSD2 genes under the effect of overexpression of the SERPINA-1 gene, a serine protease inhibitor, notably neutrophil elastase, and a potent anti-inflammatory agent [33,34].

2. Materials and Methods

2.1. Maintenance of the JP7 Cell Line

The JP7 cell line, established in our laboratory according to the protocol described by Tissent and al. [35], originated from the transformation of human lymphocytes by the Epstein-Barr virus. These transformed lymphocytes were then stabilized by fusion with the murine X63Ag8 myeloma cell line derived from BALB/c mice. The resulting hetero-lymphoblastoid cells are maintained in continuous culture through successive passages in RPMI 1640 culture medium supplemented with 10% fetal bovine serum (FBS), 200 mM glutamine, and 100 IU/mL penicillin and streptomycin. Cultures are maintained in sterile containers and incubated at 37 °C in a humidified atmosphere with 5% CO2.

2.2. Establishment, Identification, and Selection of JP7pSer+ and JP7pSer- Cell Lines

JP7 cells were cultured in a 6-well plate at a density of 5 × 105 cells/well in 3 mL of culture medium. After 24 h, the cells were washed and supplemented with 1 mL/well of culture medium containing 1 µg of either the pCMV6-SERPINA1-GFP or pCMV6-GFP plasmid vector, along with 8 µL of transfection reagent (CANFAST, Canvaxbiotech; Valladolid, Spain). Cells transfected with pCMV6-SERPINA1-GFP were designated JP7pSer+, while cells transfected with pCMV6-GFP were designated JP7pSer-. Following a 5-h incubation period, the cells were washed by centrifugation with RPMI medium without FBS and returned to culture as described above, with the addition of the selective antibiotic G418 at 400 µg/mL. After 48 h, the cells were harvested for downstream assays. Cell condition and proliferation were monitored by microscopy (EVOS™ FL Digital Inverted Fluorescence Microscope). Under these selective conditions, only transfected cells that had integrated the G418 resistance gene and expressed green fluorescent protein (GFP) were able to proliferate, as indicated by green fluorescence. Transfection efficiency was assessed by determining the percentage of fluorescent cells using microscopy (EVOS™ FL Digital Inverted Fluorescence Microscope) at 200-x magnification, and SERPINA1 gene expression was confirmed by qRT-PCR.

2.3. Evaluation of the Effect of Serpina-1 Gene Transfection on Cell Lines in Culture

2.3.1. Cell Proliferation

The colorimetric study using Trypan blue exclusion, 20 × 103 cells/well were seeded into six 96-well plates. For the enzymatic study employing MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Toronto Research Chemicals; Vaughan, ON, Canada), 5 × 103 cells/well were seeded into three 96-well plates. The JP7pSer+ and JP7pSer- cells were cultured for 6 days under standard conditions as previously described, but without the selective G418. The culture medium was not renewed during this period. Cell viability and proliferation were assessed daily and in parallel using both the Trypan blue exclusion and MTT assays. All tests were performed in triplicate across three independent experiments. The percentage of viable cells, as determined by Trypan blue exclusion, was quantified by microscopy using a Malassez counting chamber. The enzymatic activity of the cells was evaluated by spectrophotometric measurement at 570 nm, which quantifies the optical density of the formazan product resulting from the reduction of MTT by succinate dehydrogenase, a mitochondrial metabolic enzyme.

2.3.2. Gene Expression of SERPINA-1, Furin, IL-6 and NSD2

The total RNA from the cell lines was extracted using the kit (PRImeZOL RNA Isolation, Canvaxbiotech; Valladolid, Spain), in accordance with the manufacturer’s instructions, and quantified by spectrophotometry (MaestroNano, MaestroGen, Hsinchu City, Taiwan). A quantity of 250 ng/μl of RNA was used for each qRT-PCR using the kit one-step (qMAXSen, Canvaxbiotech; Valladolid, Spain). The specific primers used for the genes (Table 1), provided and reconstituted according to the manufacturer’s recommendations (NeoBiotech; Nanterre—France). Amplification in 35 cycles was performed in a thermocycler (BIO-RAD CFX 96, C1000) calibrated for SYBR Green fluorescence quantification. The qRT-PCR experiments were performed in triplicate daily for 5 days. The results obtained were normalized separately to the human beta-actin reference gene according to the Livak method before calculating the means of the observed expression ratios each day [36].

2.3.3. Membrane Expression of the IGF-1 Receptor

The membrane expression of IGF-1R was measured by flow cytometry (BD FACS Lyric Flow Cytometry System, BD Biosciences). For this purpose, the JP7pSer+ and JP7pSer- cells, suspended at 30 × 103/100 µL in RPMI-1640 medium without additives, were incubated in 5 mL tubes with 10 µL of the anti-CD221-PE monoclonal antibody (Clone IH7, Origène Technologie; Rockville, MD, USA) or the non-specific anti-IgG1-PE isotype antibody (Clone HP6001, BD Pharmingen; San Diego, CA, USA). After 30 min of incubation in the dark at room temperature, the cells were washed 3 times by centrifugation with RPMI-1640 medium and then suspended for flow cytometer analysis.

2.4. Statistical Analysis

The normality of the distribution of values was verified by the Shapiro-Wilcoxon test. Statistical differences according to the t test, Welch, and Fisher tests were verified using GraphPad Prism8 software (version 8.0.2).

3. Results

3.1. Establishment, Identification, and Selection of JP7pSer+ and JP7pSer- Cell Lines

The selection of transfected cell lines was based on observations from both fluorescence microscopy and the quantification of mRNA for A1AT. Culture wells containing fluorescent cells at greater than 95% and an SERPINA-1 gene mRNA level at least equal to 1 relative to the Beta-actin reference gene were selected.

3.1.1. Microscopy

To evaluate the stability of the JP7pSer- and JP7pSer+ model lines, the cells were observed under a fluorescence microscope and digitized to have more than 95% of the fluorescent cells, in order to perform the genetic expression and proliferation tests.
After 48 h of culture with selective agent G418 to generate stable cell lines, more than 95% of JP7pSer+ and JP7pSer- cells emitted green fluorescence under excitation at 490 nm (blue light). These results show that JP7pSer+ are fluorescent and express GFP; this expression is indicative of A1AT expression in the JP7pSer+ line because the same cytomegalovirus promoter governs both the SERPINA-1 and GFP genes (Figure 1).
3.1.2. qRT-PCR
qRT-PCR analysis was performed on several wells of cells to measure SERPINA-1 gene expression in both JP7pSer+ and JP7pSer- cell clones in order to confirm SERPINA-1 overexpression by JP7pSer+ cells. Beta-actin was used as the housekeeping gene in this experiment.
The average expression of the SERPINA-1 gene in JP7pSer- cells is 1.2, while that of JP7-pSer+ cells is 226, relative to the expression of the Beta-actin housekeeping gene (Figure 2). These results therefore confirm the overexpression of the SERPINA-1 in the JP7pSer+ cell line.

3.2. Evaluation of the Effect of SERPINA-1 Gene Transfection on Cultured Cell Lines

3.2.1. Cell Proliferation

The proliferation test was designed to determine whether the proliferative capacity of JP7pSer+ cells overexpressing SERPINA-1 is lower than that of JP7pSer- cells.
To confirm this, two proliferation tests were used: a colorimetric method based on counting viable cells with trypan blue (Figure 3A), and an enzymatic method by MTT based on the metabolic activity of the cells (Figure 3B).
The colorimetric proliferation assay of JP7pSer+ cells shows the same trends, with a decrease in proliferation of more than 64% in 4 days and more than 91% after 6 days (Figure 3A). The proliferation measured by MTT of JP7pSer+ cells decreases by 38% in 4 days and by more than 85% in terms of 6 days compared to JP7pSer- cells (Figure 3B).

3.2.2. Kinetics of Furin, IL-6, and NSD2 Gene Expression

The kinetic study of the expression by qRT-PCR of Furin, IL-6, and NSD2 was designed to observe the inhibitor effect of the SERPINA-1 gene on JP7pSer+ cells compared to JP7pSer- cells. These gene expressions of Furin, IL-6, and NSD2 were calculated using the Livak and Schmittgen method [36], relative to the expression of the Beta actin gene.
The qRT-PCR analysis revealed that the JP7pSer+/JP7pSer- ratio of Furin gene expression was 4.88 on the 1st day and became 1.01 on the 3rd day, decreasing to 0.37 on the 6th day of culture. However, the same cellular ratio of IL-6 gene expression was 3.07 on the 1st day and decreased to 0.74 on the 3rd day, reaching 0.47 on the 6th day. As for NSD2 expression, the JP7pSer+/JP7pSer- ratio was 1.59 on the 1st day and decreased to 0.7 on the 3rd day, continuing to decrease to 0.51 on the 6th day (Figure 4). Three experiments were conducted to study the effect of the SERPINA-1 gene over-expression on the expression kinetics of the three genes encoding Furin, IL-6, and NSD2. All three experiments yielded reproducible and highly similar results.
These results indicate an influence of SERPINA-1 gene over-expression on the kinetics of Furin, IL-6, and NSD2 expression, with an initial increase in expression followed by a progressive decrease.

3.2.3. Membrane Expression of the IGF-1 Receptor (IGF-1R, CD221)

This section will likely examine the inhibitor effect of the SERPINA-1 gene on the surface expression of the Insulin-like Growth Factor 1 Receptor (IGF-1R), also known as CD221.
Cytofluorimetric analysis using anti-CD221-PE showed a near-absence of labelling in JP7 cells. The JP7pSer- and JP7pSer+ cell lines showed low and nearly similar percentages with JP7pSer- (3.9%) vs. JP7pSer+ (3.46%), t-test = 1.2 with a p-value = 0.22 > 0.05, not significant. The average fluorescence intensity over three measurements for each cell line shows that JP7pSer- cells have an average MFI = 278.33 AU, while for JP7pSer+, the average MFI = 209.33 AU, with different confidence intervals CI99: [272.48; 284.17] for JP7pSer- and CI99: [177.31; 241.34] for JP7pSer+. The p-value < 0.01 with a significance threshold of 0.05. These results indicate that CD221 expression is globally very low in both JP7pSer- and JP7pSer+ cell lines, but with a 25% decrease in JP7pSer+ compared to JP7pSer- (Figure 5E).

4. Discussion

The proliferative JP7 cell line, derived from a fusion between a human EBV-transformed lymphocyte and a murine myeloma, has been used as a malignant lymphoblastoid model comparable to Burkitt’s lymphoma [37] to study the effects of SERPINA-1 gene expression on cell proliferation and the expression of Furin, IL-6, and NSD2 biomarkers. JP7pSer+ cells, overexpressing SERPINA-1, and JP7pSer- control cells were generated using the vector under the control of the cytomegalovirus promoter. This approach allowed us to isolate the effect of SERPINA-1 overexpression in JP7pSER+ cells, while maintaining identical experimental conditions for both cell lines. This model helps to study the effect of SERPINA-1 expression on various cellular processes. Alpha-1 antitrypsin (A1AT), a 52 kDa glycoprotein, belongs to the serine protease inhibitor superfamily and is encoded by the SERPINA-1 locus, also known as the Pi locus, located on the long arm of chromosome 14 [38]. However, studies show the involvement of A1AT in tumors such as non-small cell lung cancer [33]. Cancer cells exhibit variable expression of the SERPINA-1 gene, often resulting in limited production of endogenous A1AT protein [39]. This suggests the potential role of A1AT in intracellular signaling pathways or other biological processes within tumor cells [40]. However, in tumor contexts, serine proteases, which can be regulated by A1AT, could modulate the mobilization of hematopoietic progenitor cells into the blood [41]. Current results have revealed that overexpression of the SERPINA-1 gene resulted in slower proliferation of JP7pSer+ cells compared to JP7pSer- cells.
This observation suggests that A1AT may possess anti-proliferative properties in this Burkitt’s lymphoma comparable model, which contradicts previous studies that implicate A1AT in tumor progression in other cancer types. The sustained anti-proteolysis properties of A1AT are important given its physiological role [42]. In this context, our results support a potential role for A1AT in regulating the growth and proliferation of malignant lymphoid cells. Given the well-established role of Furin in cancer progression, we evaluated the impact of SERPINA-1 overexpression on Furin gene expression. Furin, a proprotein convertase, plays a critical role in the activation of various proteins involved in cell growth, angiogenesis, and metastasis [43]. Our results revealed that JP7pSer+ cells exhibited a significant decrease in Furin gene expression compared to JP7pSer- cells after three days of culture. These results suggest that A1AT may exert its anti-proliferative effects by modulating Furin expression, which could affect the activation of downstream proteins that are involved in cancer progression. Therefore, further research is needed to identify the precise molecular mechanisms by which A1AT regulates Furin gene expression and to evaluate the potential therapeutic significance of this process in Burkitt’s lymphoma. However, in the JP7pSer- and JP7pSer+ cell lines, the expression of Furin substrate IGF-1R [27] is present in a minority of cells in both lines, suggesting that this signaling pathway may not play a major role in this cell model. In contrast, in the JP7pSer+ cell line, the mean fluorescence intensity of IGF-1R expression was reduced by 67%, suggesting that SERPINA-1 overexpression even decreases IGF-1R expression on the cell surface. Thus, this reduction in IGF-1R expression on the surface of JP7pSer+ cells provides an indirect argument in favor of the biological activity of A1AT, demonstrating not only its impact on a specific signaling pathway but also on cell proliferation as assessed by enzymatic and colorimetric methods. Other studies show a link between Furin and the IGF-1R axis [31,44]. It is well established that a functional IGF-1R is required for cell growth and plays a crucial role in the survival of various transformed cells in vitro and in vivo [44]. In addition, in the current study, we evaluated the impact of SERPINA-1 overexpression on the expression of IL-6, a cytokine known to promote the growth and survival of Burkitt’s lymphoma cells [45]. Interleukin-6, a pleiotropic cytokine, plays a crucial role in various biological processes, including immune regulation, inflammation, and tumor growth. IL-6 can promote tumor cell proliferation, survival, and angiogenesis, thereby contributing to cancer progression [13]. IL-6 can promote tumor growth via an auto-amplification loop for Th17 cells in the tumor microenvironment. These results suggest that the role of IL-17 in cancer is context- and system-dependent, like many other cytokines such as TNF-α [13]. In contrast, A1AT has demonstrated its ability to interact directly with cytokines such as TNF-α or IL-1 in inflammatory cells, thereby modulating their actions [46]. The latter are pro-inflammatory cytokines produced in response to various stimuli such as infections or tissue damage and stimulate the production of IL-6 [47]. In this study, we observed that SERPINA-1 gene overexpression resulted in a significant decrease in IL-6 gene expression in JP7pSer+ cells compared to JP7pSer- cells. Current observations suggest that A1AT could potentially regulate IL-6 gene expression in Burkitt’s lymphoma comparable model cells, thus offering insight into its potential role in controlling the tumor microenvironment. As for the methyltransferase NSD2/MMSET, it is an enzyme responsible for H3K36 methylation and is involved in various cellular processes, including DNA repair and transcription regulation [48]. Importantly, the study observed a decrease in methyltransferase NSD2/MMSET expression in JP7pSer+ cells compared to JP7pSer- cells. Although the essential role of NSD2 in JAK-STAT signaling via IL-6 activation has been well established, it is also responsible for cancer cell proliferation, which can be modulated with available treatments [49]. These results suggest that A1AT may influence the expression of methyltransferases, potentially affecting epigenetic regulation.
The link between IL-6 and Furin is established via the cytokines TGF-β1, IL-1β, and TNF-α, which are substrates of Furin and major activators of IL-6 expression. Furthermore, peritoneal macrophages from Furin knockout mice have shown reproducible positive regulation of numerous expressed genes, such as Serpinb1a, IL-6, and IL-1β [9,43]. Further studies are needed to further characterize the molecular mechanisms underlying these regulations, providing comprehensive information on the impact of A1AT on various cell-signaling pathways and its potential effects on cancer development and progression.

5. Conclusions

Considering all these results, our study provides evidence that SERPINA-1 overexpression may exert anti-proliferative effects on Burkitt’s lymphoma model cells by modulating the expression of various key proteins involved in cancer progression. More specifically, we observed that SERPINA-1 overexpression resulted in decreased expression of Furin, IL-6, and NSD2, concomitant factors involved in tumor cell proliferation, survival, and epigenetic regulation. Overall, these results suggest that A1AT could potentially serve as a therapeutic target for the treatment of Burkitt’s lymphoma, thus warranting further research, proteomic, epigenetic, and transcriptomic, to fully elucidate its mechanisms of action and evaluate its efficacy in preclinical and clinical studies.

Author Contributions

Conceptualization, N.T., A.T. and N.H.; Methodology, N.T. and N.H.; Validation, A.T. and N.H.; Formal analysis, N.T. and N.H.; Resources, N.T. and H.A.; Data curation, N.T.; Writing—original draft, N.T.; Writing—review & editing, N.T. and N.H.; Visualization, N.T. and A.T.; Supervision, N.H.; Project administration, N.H.; Funding acquisition, H.A. and N.H. All authors have read and agreed to the published version of the manuscript.

Funding

The National Blood Transfusion Center of Morocco and the Laboratory of Cellular and Molecular Inflammatory, Degenerative, and Oncological Pathophysiology (LPCMIDO) funded this research.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The original contributions presented in this study are included in the article. Further inquiries can be directed to the corresponding authors.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

IGF-1Insulin-like growth factor-1
PI3KPhosphatidylinositol 3-kinase
AKTProtein kinase B
mTORMammalian target of rapamycin
MAPKMitogen-activated protein kinase
ERKExtracellular signal-regulated kinases
JAKJanus kinases
STATSignal transducer and activator of transcription proteins
IGF-1RInsulin-like growth factor-1 receptor
IL-6Interleukin 6
TNF-αTumor necrosis factor
NSD2Probable histone-lysine N-methyltransferase
EBVEpstein-Barr virus
STAT3Signal transducer and activator of transcription 3
LysLysine
ArgArginine
TGF-β1Transforming growth factor beta 1
PCProprotein convertase
A1ATAlpha-1 antitrypsin
Alpha- 1PDXAlpha-1 antitrypsin Portland variant
PACE4Proprotein convertase subtilisin/kexin type 6
PC7Proprotein convertase subtilisin/kexin type 7
CMVCytomegalovirus
GFPGreen fluorescent protein
mRNAMessenger ribonucleic acid
qRT-PCR Quantitative Reverse Transcription—Poly Chain Réaction
CI confidence interval

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Biologics 05 00022 g001
Figure 1. JP7pSer- (A) and JP7pSer+ (B) cells observed under a microscope at 200× magnification. The majority of the cells under 490 nm excitation emit the green fluorescence of the GFP protein. Scale 200 µm. (C) Graphical representation of the percentages of fluorescent and non-fluorescent cells in the two cell lines JP7pSer- and JP7pSer+ treated by ImageJ. Data are expressed as mean ± standard deviation of at least three independent experiments, and significant differences between the different groups in each cell line are indicated by **** p-value < 0.0001.
Figure 1. JP7pSer- (A) and JP7pSer+ (B) cells observed under a microscope at 200× magnification. The majority of the cells under 490 nm excitation emit the green fluorescence of the GFP protein. Scale 200 µm. (C) Graphical representation of the percentages of fluorescent and non-fluorescent cells in the two cell lines JP7pSer- and JP7pSer+ treated by ImageJ. Data are expressed as mean ± standard deviation of at least three independent experiments, and significant differences between the different groups in each cell line are indicated by **** p-value < 0.0001.
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Figure 2. Relative expression of the SERPINA-1 gene in JP7pSer- and JP7pSer+ cells referred to the expression of Beta-actin housekeeping gene. Data are mean ± SD of at least three independent experiments, and significant differences between the two cell lines were shown as **** p-value < 0.0001.
Figure 2. Relative expression of the SERPINA-1 gene in JP7pSer- and JP7pSer+ cells referred to the expression of Beta-actin housekeeping gene. Data are mean ± SD of at least three independent experiments, and significant differences between the two cell lines were shown as **** p-value < 0.0001.
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Figure 3. (A) The proliferation of 20 × 103 cells/well of JP7pSer+ or JP7pSer- evaluated by the colorimetric Trypan blue method. (B) The proliferation of 5 × 103 cells/well of the two cell lines, measured by enzymatic method (MTT). O.D: Optical density. Data are mean ± SD of at least three independent experiments and significant differences between the two cell lines, with p < 0.05 for both experiments.
Figure 3. (A) The proliferation of 20 × 103 cells/well of JP7pSer+ or JP7pSer- evaluated by the colorimetric Trypan blue method. (B) The proliferation of 5 × 103 cells/well of the two cell lines, measured by enzymatic method (MTT). O.D: Optical density. Data are mean ± SD of at least three independent experiments and significant differences between the two cell lines, with p < 0.05 for both experiments.
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Figure 4. Kinetics of the Furin, IL-6, and NSD2 gene expression measured by qRT-PCR in the JP7pSer+ line compared to that of the control line JP7pSer-. Data are mean ± SD of at least three independent experiments. Furin: Y = −0.04386X + 4.908; R2 = 0.7251; r = −0.85 ∈ CI95: [−1; −0.6019] p = 0.0001. IL-6: Y = −0.02834X + 3.404; R2 = 0,7463; r = −0.86 ∈ CI95: [−1; −0.7463 ] p = 0.0001. NSD2: Y = −0.01150X + 1.661; R2 = 0.6295; r = −0.79 ∈ CI95: [−1; −0.7071] p = 0.0004.
Figure 4. Kinetics of the Furin, IL-6, and NSD2 gene expression measured by qRT-PCR in the JP7pSer+ line compared to that of the control line JP7pSer-. Data are mean ± SD of at least three independent experiments. Furin: Y = −0.04386X + 4.908; R2 = 0.7251; r = −0.85 ∈ CI95: [−1; −0.6019] p = 0.0001. IL-6: Y = −0.02834X + 3.404; R2 = 0,7463; r = −0.86 ∈ CI95: [−1; −0.7463 ] p = 0.0001. NSD2: Y = −0.01150X + 1.661; R2 = 0.6295; r = −0.79 ∈ CI95: [−1; −0.7071] p = 0.0004.
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Figure 5. Graphs of one of the three flow cytometry measurements analysis of membrane expression of IGF-1R by JP7pSer- (A) vs. JP7pSer+ (B) cells, and fluorescence intensity of JP7pSer- (C) vs. JP7pSer+ (D). Mean fluorescence intensity (MFI) was calculated from the three measurements (E), and the average MFI was 278.33 AU for JP7pSer- cells with a CI99: [272.48; 284.17], and an average MFI of 209.33 for JP7pSer+ cells with a CI99: [177.31; 241.34]. The ** p-value < 0.01, with a significance threshold of 0.05.
Figure 5. Graphs of one of the three flow cytometry measurements analysis of membrane expression of IGF-1R by JP7pSer- (A) vs. JP7pSer+ (B) cells, and fluorescence intensity of JP7pSer- (C) vs. JP7pSer+ (D). Mean fluorescence intensity (MFI) was calculated from the three measurements (E), and the average MFI was 278.33 AU for JP7pSer- cells with a CI99: [272.48; 284.17], and an average MFI of 209.33 for JP7pSer+ cells with a CI99: [177.31; 241.34]. The ** p-value < 0.01, with a significance threshold of 0.05.
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Table 1. Primers used during one-step qRT-PCR for Furin, IL-6, NSD2, and Beta-actin genes.
Table 1. Primers used during one-step qRT-PCR for Furin, IL-6, NSD2, and Beta-actin genes.
GenesForwardReverse
SERPINA-15′-GGCTGACACTCACGATGAAA-3′5′-GTGTCCCCGAAGTTGACAGT-3′
Furin5′-GCCCAGAATTGGACCACAGT-35′-TCCCGATGTCTTTGGGCTC-3′
IL-65′-AGACAGCCACTCACCTCTTCAG-3′5′-TTCTGCCAGTGCCTCTTTGCTG-3′
NSD25′-AATATGACTCCTTGCTGGAGCAGG-3′5′-ATTTCAACAGGTGGTCTTTGTCTC-3′
Beta-actin5′-TGGAATCCTGTGGCATCCATGAAAC-35′-TAAAACGCAGCTCAGTAACAGTCC-3′
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MDPI and ACS Style

Tassou, N.; Anibat, H.; Tissent, A.; Habti, N. Correlation of SERPINA-1 Gene Over-Expression with Inhibition of Cell Proliferation and Modulation of the Expression of IL-6, Furin, and NSD2 Genes. Biologics 2025, 5, 22. https://doi.org/10.3390/biologics5030022

AMA Style

Tassou N, Anibat H, Tissent A, Habti N. Correlation of SERPINA-1 Gene Over-Expression with Inhibition of Cell Proliferation and Modulation of the Expression of IL-6, Furin, and NSD2 Genes. Biologics. 2025; 5(3):22. https://doi.org/10.3390/biologics5030022

Chicago/Turabian Style

Tassou, Nassim, Hajar Anibat, Ahmed Tissent, and Norddine Habti. 2025. "Correlation of SERPINA-1 Gene Over-Expression with Inhibition of Cell Proliferation and Modulation of the Expression of IL-6, Furin, and NSD2 Genes" Biologics 5, no. 3: 22. https://doi.org/10.3390/biologics5030022

APA Style

Tassou, N., Anibat, H., Tissent, A., & Habti, N. (2025). Correlation of SERPINA-1 Gene Over-Expression with Inhibition of Cell Proliferation and Modulation of the Expression of IL-6, Furin, and NSD2 Genes. Biologics, 5(3), 22. https://doi.org/10.3390/biologics5030022

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