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Brief Report

Comparison of SARS-CoV-2 Antigen Tests in Asymptomatic Testing of Passengers at German Airports under Time Constraints: Application of Three Different Antigen Test Formats

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CENTOGENE GmbH, 18055 Rostock, Germany
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Dr Bauer Laboratoriums GmbH, 18055 Rostock, Germany
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Medizinisches Versorgungszentrum Labor Limbach Vorpommern Rügen, 18435 Stralsund, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Tohru Suzuki
COVID 2021, 1(3), 546-554; https://doi.org/10.3390/covid1030046
Received: 7 July 2021 / Revised: 15 October 2021 / Accepted: 29 October 2021 / Published: 4 November 2021
People infected asymptomatically with SARS-CoV-2 can spread the virus very efficiently. To break infection chains, massive testing efforts are underway. While the value of RT-PCR in asymptomatic patients is established, point-of-care (POC) antigen tests against SARS-CoV-2 are considered inferior to RT-PCR in terms of sensitivity and specificity but have demonstrated utility, mostly in symptomatic patients. We compared the performance of three different antigen tests with colorimetric (Roche), fluorometric (Quidel Sofia 2), and instrument-based chemiluminescent (Fujirebio Lumipulse® G) readout. Sensitivities for Roche, Quidel, and Fujirebio were 62.5%, 90.9%, 97.5% (≤ct 26); 43.8%, 90.9%, 95.1% (≤ct 30); and 4.3%, 0.0%, 57.6% (˃ct 30), respectively. The two assays with increased sensitivity were employed to screen > 35,000 passengers at German airports under time constraints. Under real-world conditions, the rate of false positives was low: 0.15% (Quidel) and 0.06% for the instrument based Fujirebio assay. Our study exemplifies that antigen tests with enhanced detection methods have an acceptable sensitivity of >90% in samples containing SARS-CoV-2 RNA that are considered to be infectious. Therefore, our results support the view of the WHO that discourages the use of antigen assays with a sensitivity of “only” 80% for screening travelers. View Full-Text
Keywords: SARS-CoV-2; RT-PCR; asymptomatic; antigen test; point-of-care SARS-CoV-2; RT-PCR; asymptomatic; antigen test; point-of-care
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MDPI and ACS Style

Pradas, L.; Viceconte, N.; Hannen, J.; Schölz, C.; Schubert, A.; Knote, R.; Wille, L.; Kleinow, P.; Heuer, A.; Weckesser, V.; Hartkamp, J.; Schaefer, S.; Bauer, P. Comparison of SARS-CoV-2 Antigen Tests in Asymptomatic Testing of Passengers at German Airports under Time Constraints: Application of Three Different Antigen Test Formats. COVID 2021, 1, 546-554. https://doi.org/10.3390/covid1030046

AMA Style

Pradas L, Viceconte N, Hannen J, Schölz C, Schubert A, Knote R, Wille L, Kleinow P, Heuer A, Weckesser V, Hartkamp J, Schaefer S, Bauer P. Comparison of SARS-CoV-2 Antigen Tests in Asymptomatic Testing of Passengers at German Airports under Time Constraints: Application of Three Different Antigen Test Formats. COVID. 2021; 1(3):546-554. https://doi.org/10.3390/covid1030046

Chicago/Turabian Style

Pradas, Laura, Nikenza Viceconte, Jennifer Hannen, Christian Schölz, Axel Schubert, Robert Knote, Leon Wille, Peter Kleinow, Andreas Heuer, Volkmar Weckesser, Jörg Hartkamp, Stephan Schaefer, and Peter Bauer. 2021. "Comparison of SARS-CoV-2 Antigen Tests in Asymptomatic Testing of Passengers at German Airports under Time Constraints: Application of Three Different Antigen Test Formats" COVID 1, no. 3: 546-554. https://doi.org/10.3390/covid1030046

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