A Major Facilitator Superfamily Transporter Contributes to Ergot Alkaloid Accumulation but Not Secretion in Aspergillus leporis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Heterologous Expression of easT in A. fumigatus Strain LA
2.2. Localization of easT in A. fumigatus
2.3. Knockout of easT in A. leporis
3. Results and Discussion
3.1. Analysis of easT by Heterologous Expression in A. fumigatus
3.2. Localization of the Product of easT Heterologously Expressed in A. fumigatus
3.3. Analysis of easT by Gene Knockout in A. leporis
3.4. Differences in Ergot Alkaloid Accumulation in Engineered A. fumigatus Compared to A. leporis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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nmol Alkaloid/g Dry Weight Fungus (Mean ± SE) | ||||
---|---|---|---|---|
Solid Phase | Liquid Phase | |||
Alkaloid | A. fumigatus Strain LA | Strain LA + easT | A. fumigatus Strain LA | Strain LA + easT |
Chanoclavine-I | 66 ± 9 A 1 | 174 ± 18 B | 14 ± 1 a | 4 ± 0.4 b |
Agroclavine | 302 ± 51 A | 4891 ± 634 B | 171 ± 11 a | 37 ± 8 b |
Setoclavine | 37 ± 4 A | 303 ± 43 B | 3 ± 0.3 a | 2 ± 0.2 b |
Lysergic acid | 19 ± 4 | n.d. 2 | 5 ± 0.5 | n.d. |
Total | 424 ± 67 A | 5367 ± 690 B | 192 ± 12 a | 43 ± 9 b |
nmol Alkaloid/g Dry Weight Fungus (Mean ± SE) | ||||
---|---|---|---|---|
Solid Phase | Liquid Phase | |||
Alkaloid | Wild Type | easT Knockout | Wild Type | easT Knockout |
Chanoclavine-I | 6 ± 0.7 A 1 | 4 ± 0.4 A | 20 ± 2 a | 19 ± 0.7 a |
Lysergic acid | 2 ± 0.2 A | 2 ± 0.2 A | 3 ± 0.3 a | 2 ± 0.2 a |
Lysergyl-alanine | 4 ± 0.3 A | 3 ± 0.2 B | 11 ± 0.9 a | 9 ± 0.4 a |
Ergonovine | 2 ± 0.1 A | 1 ± 0.1 B | 6 ± 0.7 a | 6 ± 0.3 a |
LAH | 185 ± 17 A | 144 ± 35 B | 427 ± 40 a | 401 ± 21 a |
Ergine | 61 ± 6 A | 46 ± 4 B | 237 ± 20 a | 174 ± 6 b |
LAH + ergine | 246 ± 22 A | 189 ± 13 B | 665 ± 55 a | 575 ± 23 a |
Total | 259 ± 23 A | 200 ± 14 B | 705 ± 59 a | 611 ± 25 a |
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Jones, A.M.; Davis, K.A.; Panaccione, D.G. A Major Facilitator Superfamily Transporter Contributes to Ergot Alkaloid Accumulation but Not Secretion in Aspergillus leporis. Appl. Microbiol. 2024, 4, 406-417. https://doi.org/10.3390/applmicrobiol4010028
Jones AM, Davis KA, Panaccione DG. A Major Facilitator Superfamily Transporter Contributes to Ergot Alkaloid Accumulation but Not Secretion in Aspergillus leporis. Applied Microbiology. 2024; 4(1):406-417. https://doi.org/10.3390/applmicrobiol4010028
Chicago/Turabian StyleJones, Abigail M., Kyle A. Davis, and Daniel G. Panaccione. 2024. "A Major Facilitator Superfamily Transporter Contributes to Ergot Alkaloid Accumulation but Not Secretion in Aspergillus leporis" Applied Microbiology 4, no. 1: 406-417. https://doi.org/10.3390/applmicrobiol4010028