Sugar-Induced Cell Death in the Yeast S. cerevisiae Is Accompanied by the Release of Octanoic Acid, Which Does Not Originate from the Fatty Acid Synthesis Type II Mitochondrial System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Culture Growth
2.2. 1,2,3,-Dihydrorhodamine (DHR) Staining
2.3. SICD Assay and Flow Cytometry
2.4. Preparation of FAME for Gas Chromatography
2.5. Mass Spectrometry and Gas Chromatography
3. Results and Discussion
4. Conclusions
- (1)
- OA originated from mitochondrial FAS-II systems is not the cause of SICD;
- (2)
- mitochondria are not the site of initiation of primary necrosis in the form of SICD.
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Avtukh, A.; Baskunov, B.; Keshelava, V.; Valiakhmetov, A. Sugar-Induced Cell Death in the Yeast S. cerevisiae Is Accompanied by the Release of Octanoic Acid, Which Does Not Originate from the Fatty Acid Synthesis Type II Mitochondrial System. Appl. Microbiol. 2023, 3, 722-734. https://doi.org/10.3390/applmicrobiol3030050
Avtukh A, Baskunov B, Keshelava V, Valiakhmetov A. Sugar-Induced Cell Death in the Yeast S. cerevisiae Is Accompanied by the Release of Octanoic Acid, Which Does Not Originate from the Fatty Acid Synthesis Type II Mitochondrial System. Applied Microbiology. 2023; 3(3):722-734. https://doi.org/10.3390/applmicrobiol3030050
Chicago/Turabian StyleAvtukh, Alexander, Boris Baskunov, Varlam Keshelava, and Airat Valiakhmetov. 2023. "Sugar-Induced Cell Death in the Yeast S. cerevisiae Is Accompanied by the Release of Octanoic Acid, Which Does Not Originate from the Fatty Acid Synthesis Type II Mitochondrial System" Applied Microbiology 3, no. 3: 722-734. https://doi.org/10.3390/applmicrobiol3030050