Short-Term Effect of Green Waste and Sludge Amendment on Soil Microbial Diversity and Volatile Organic Compound Emissions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Site Description
2.2. Microcosms Preparation
Timing of the VOCs Measurements
2.3. PTR-QiTOF-MS Measurement Set Up
2.4. Microbial Analyses
2.5. Statistical Analysis
2.5.1. VOCs Statistical Analyses
2.5.2. Microbial Statistical Analyses
2.5.3. VOC and Phyla Correlation Analysis
3. Results
3.1. Microbial Biomass and Diversity
3.2. Prokaryotic Relative Abundance
3.3. VOC Emissions from the Microcosms
4. Discussion
4.1. Microbial Diversity Manipulation
4.2. Effect of the GWS Amendment on the Microbial Community
4.3. Effect of the GWS Amendment on the Summed VOC Emissions
4.4. Prokaryotic VOC Emissions Profile Evolution after the Fresh GWS Amendment
4.5. Metabolic Pathways of the Most Emitted VOCs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Soil Characteristic and Unit | CN | GWS |
---|---|---|
Clay (<2 µm) g/kg | 199 | |
Fine silt (2/20 µm) g/kg | 263 | |
Coarse silt (20/20 µm) g/kg | 442 | |
Fine sand (50/200 µm) g/kg | 87 | |
Coarse sand (200/2000 µm) g/kg | 9 | |
Organic Carbon g/kg | 9.36 | 15.2 |
Total N g/kg | 0.92 | 1.55 |
C/N | 10.1 | 9.82 |
Organic Matter g/kg | 16.2 | 26.3 |
pH | 6.90 | 7.04 |
CEC cmol+/kg | 9.52 | 11.3 |
P2O5 g/100 g | 0.06 | 0.21 |
GWS Characteristic and Unit | Value | |
---|---|---|
Dry Matter (DM) | % | 67.2 ± 1.3 |
Organic Carbon | g kg−1 DM | 257 ± 2 |
Total N | g kg−1 DM | 22.5 ± 0.5 |
P2O5 (Olsen) | g kg−1 DM | 0.8 ± 0.1 |
C/N | 13.2 ± 0.5 | |
pH (water) | 7.7 ± 0.1 | |
Molecular Biomass | µg of DNA g−1 of soil | 54.9 ± 19.1 |
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Abis, L.; Sadet-Bourgeteau, S.; Lebrun, B.; Ciuraru, R.; Lafouge, F.; Nowak, V.; Tripied, J.; Houot, S.; Maron, P.A.; Loubet, B. Short-Term Effect of Green Waste and Sludge Amendment on Soil Microbial Diversity and Volatile Organic Compound Emissions. Appl. Microbiol. 2021, 1, 123-141. https://doi.org/10.3390/applmicrobiol1010010
Abis L, Sadet-Bourgeteau S, Lebrun B, Ciuraru R, Lafouge F, Nowak V, Tripied J, Houot S, Maron PA, Loubet B. Short-Term Effect of Green Waste and Sludge Amendment on Soil Microbial Diversity and Volatile Organic Compound Emissions. Applied Microbiology. 2021; 1(1):123-141. https://doi.org/10.3390/applmicrobiol1010010
Chicago/Turabian StyleAbis, Letizia, Sophie Sadet-Bourgeteau, Benjamin Lebrun, Raluca Ciuraru, Florence Lafouge, Virginie Nowak, Julie Tripied, Sabine Houot, Pierre Alain Maron, and Benjamin Loubet. 2021. "Short-Term Effect of Green Waste and Sludge Amendment on Soil Microbial Diversity and Volatile Organic Compound Emissions" Applied Microbiology 1, no. 1: 123-141. https://doi.org/10.3390/applmicrobiol1010010