Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts
Abstract
:1. Introduction
2. Material and Methods
2.1. Parasite Culture and Collection
2.2. RNA Isolation and cDNA Synthesis
2.3. Cloning and Expression of T. circumcincta Recombinant TciALDO-1 in E. coli
2.4. Purification and Gel Electrophoresis
2.5. Bioinformatics
2.6. TciALDO-1 Activity (E.C. 4.2.1.11)
2.7. ELISA
2.8. Protein Modelling and Structural Analysis of TciALDO-1
2.9. Data Analysis
3. Results
3.1. TciALDO-1 Gene Sequence
3.2. Recombinant Protein Expression
3.3. Enzyme Assays
3.4. Host Recognition
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Umair, S.; Bouchet, C.; Palevich, N.; Simpson, H. Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts. Parasitologia 2021, 1, 1-11. https://doi.org/10.3390/parasitologia1010001
Umair S, Bouchet C, Palevich N, Simpson H. Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts. Parasitologia. 2021; 1(1):1-11. https://doi.org/10.3390/parasitologia1010001
Chicago/Turabian StyleUmair, Saleh, Charlotte Bouchet, Nikola Palevich, and Heather Simpson. 2021. "Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts" Parasitologia 1, no. 1: 1-11. https://doi.org/10.3390/parasitologia1010001