Analysis of Circulating Fatty Acid Profiles in Free-Ranging and Managed Care Marine Toads (Rhinella marina) with a Comparison of Whole-Blood Vial and Whole-Blood Dried Blood Spot Card Analyses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Housing and Diet
2.3. Lab Testing Methods
2.4. Cricket Feed and Cricket Analyses
2.5. Statistical Analyses
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- International Union for Conservation of Nature (IUCN). IUCN SSC Amphibian Specialist Group. 2020. Available online: https://www.iucn-amphibians.org/ (accessed on 22 April 2022).
- Association of Zoos and Aquariums (AZA). Species Survival Plan Programs. 2020. Available online: https://www.aza.org/species-survival-plan-programs (accessed on 22 April 2022).
- Dgebuadze, Y.Y.; Sushchik, N.N.; Bashinskiy, I.V.; Makhutova, O.N.; Kalacheva, G.S.; Osipov, V.V.; Gladyshev, M.I. Fatty acid composition revealed differences in the diets of tadpoles of two amphibian species. Dokl. Biochem. Biophys. 2017, 472, 31–34. [Google Scholar] [CrossRef] [PubMed]
- Brenes-Soto, A.; Dierenfeld, E.S.; Hendriks, W.H.; Janssens, G.P.J. Gaining insights in the nutritional metabolism of amphibians: Analyzing body nutrient profiles of the African clawed frog, Xenopus Laevis. PeerJ 2019, 7, e7365. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fritz, K.A.; Whiles, M.R.; Trushenski, J.T. Subsidies of long-chain polyunsaturated from aquatic to terrestrial environments via amphibian emergence. Freshw. Biol. 2019, 64, 832–842. [Google Scholar] [CrossRef]
- Ferrie, G.N.; Allford, V.V.; Baitchman, E.; Blaner, W.S.; Crawshaw, G.; Deneault, A.; Barber, D.; Dierenfeld, E.; Fleming, G.; Gagliardo, R.; et al. Nutrition and health in amphibian husbandry. Zoo Biol. 2014, 33, 485–501. [Google Scholar] [CrossRef] [Green Version]
- Clauss, M.; Dierenfeld, E.S.; Bigley, K.E.; Wang, Y.; Ghebremeskel, K.; Hatt, J.M.; Flach, E.J.; Behlert, O.; Castell, J.C.; Streich, W.J.; et al. Fatty acid status in captive and free-ranging black rhinoceroses (Diceros bicornis). J. Anim. Physiol. Anim. Nutr. 2008, 92, 231–241. [Google Scholar] [CrossRef] [Green Version]
- Tordiffe, A.S.W.; Wachter, B.; Heinrich, S.K.; Reyers, F.; Mienie, L.J. Comparative serum fatty acid profiles of captive and free-ranging cheetahs (Acinonyx jubatus) in Namibia. PLoS ONE 2016, 11, e0167608. [Google Scholar] [CrossRef] [Green Version]
- Dass, K.; Koutsos, E.; Minter, L.J.; Ange-van Heugten, K. Analysis of fatty acid profiles in Eastern box (Terrapene carolina c arolina) and common snapping (Chelydra serpentine) turtles for wild and in-human care environments. J. Zoo Wildl. Med. 2020, 51, 478–484. [Google Scholar] [CrossRef]
- Cartland-Shaw, L.K.; Cree, A.; Skeaff, C.M.; Grimmond, N.M. Differences in dietary and plasma fatty acids between wild and captive populations of a rare reptile, the tuatara (Sphenodon punctatus). J. Comp. Physiol. B 1998, 168, 569–580. [Google Scholar] [CrossRef]
- Wright, K.M.; Whitaker, B.R. Nutritional disorders. In Amphibian Medicine and Captive Husbandry; Wright, K.M., Whitaker, B.R., Malabar, F.L., Eds.; Krieger Publishing Company: Malabar, FL, USA, 2001; pp. 73–87. [Google Scholar]
- Huang, C.; Liang, M.; Kam, Y. The fatty acid composition of oophagous tadpoles (Chirixalus eiffingeri) fed conspecific or chicken egg yolk. Comp. Biochem. Physiol. Part A 2003, 135, 329–336. [Google Scholar] [CrossRef]
- Liang, M.; Huang, C.; Kam, Y. Effects of intermittent feeding on the growth of oophagous (Chirixalus eiffingeri) and herbivorous (Chirixalus idiootocus) tadpoles from Taiwan. J. Zool. 2002, 256, 207–213. [Google Scholar] [CrossRef]
- Brindle, E.; O’Connor, K.A.; Garrett, D.A. Applications of dried blood spots in general human health studies. In Dried Blood Spots: Applications and Techniques; John Wiley & Sons, Inc.: Hoboken, NJ, USA, 2014; pp. 114–129. [Google Scholar]
- Freeman, J.D.; Rosman, L.M.; Ratcliff, J.D.; Strickland, P.T.; Graham, D.R.; Silbergeld, E.K. State of the science of dried blood spots. Clin. Chem. 2018, 64, 656–679. [Google Scholar] [CrossRef] [Green Version]
- Wood, J.; Minter, L.J.; Bibus, D.; Stoskopf, M.; Fellner, V.; Ange-van Heugten, K. Comparison of African savanna elephant (Loxodonta africana) fatty acid profiles in whole blood, whole blood dried on blood spot cards, serum, and plasma. PeerJ 2021, 9, e12650. [Google Scholar] [CrossRef]
- Koutsos, E.; Minter, L.J.; Ange-van Heugten, K.D.; Mejia-Fava, J.; Harmes, C. Blood fatty acid profiles of juvenile wild green turtles (Chelonia mydas) and Kemp’s ridley turtles (Lepidochelys kempii). J. Zoo Wildl. Med. 2021, 52, 610–617. [Google Scholar] [CrossRef]
- Lever, C. The Cane Toad. In The History and Ecology of a Successful Colonist; Westbury Publishing: Nottinghamshire, UK, 2001; pp. 6–7. [Google Scholar]
- Reeves, M.P. A retrospective report of 90 dogs with suspected cane toad (Bufo marinus) toxicity. Aust. Vet. J. 2004, 82, 608–611. [Google Scholar] [CrossRef]
- Global Invasive Species Database (GISD). Rhinella marina. 2005. Available online: http://www.issg.org/database/species/ecology.asp?si=113&fr=1&sts=sss&lang=E (accessed on 20 June 2022).
- Shine, R. The ecological impact of invasive cane toads (Bufo marinus) in Australia. Q. Rev. Biol. 2010, 85, 253–291. [Google Scholar] [CrossRef]
- Yeager, A.; Commito, J.; Wilson, A.; Bower, D.; Schwarzkopf, L. Sex, light, and sound: Location and combination of multiple attractants affect probability of cane toad (Rhinella marina) capture. J. Pest Sci. 2014, 87, 323–329. [Google Scholar] [CrossRef] [Green Version]
- Archibald, K.E.; Harrison, T.; Troan, B.; Smith, D.; Minter, L.J. Effects of Multi-radiance Low-Level Laser Therapy and Topical Silver Sulfadiazine on Healing Characteristics of Dermal Wounds in Marine Toads (Rhinella marina). Vet. Med. Int. 2020, 2020, 8888328. [Google Scholar] [CrossRef]
- Freel, T.A.; Koutsos, E.A.; Minter, L.J.; Tollefson, T.N.; Ridgley, F.N.; Smith, D.; Scott, H.; Ange-van Heugten, K.D. Cane toad (Rhinella marina) vitamin A, vitamin E, and carotenoid kinetics. Zoo Biol. 2022, 41, 34–43. [Google Scholar] [CrossRef]
- Freel, T.A.; Koutsos, E.A.; Minter, L.J.; Tollefson, T.N.; Ridgley, F.N.; Brown, J.L.; Smith, D.; Scott, H.; Ange-van Heugten, K.D. Urinary corticosterone concentrations in free-ranging and human-managed cane toads (Rhinella marina). J. Zoo Wildl. Med. 2021, 52, 1234–1240. [Google Scholar] [CrossRef]
- Griffioen, J.A.; Trosclair, M.; Minter, L.J.; Vanetten, C.; Harrison, T.M. Comparison of Dilution on Eastern Box Turtles (Terrapene carolina) and Marine Toads (Bufo marinus) Blood Parameters as Measured on a Portable Chemistry Analyzer. Vet. Med. Int. 2020, 2020, 8843058. [Google Scholar] [CrossRef]
- Scott, G.; Louis, M.M.; Balko, J.A.; Bublitz, C.M.; Troan, B.V.; Baynes, R.E.; Smith, D.; Minter, L.J. Pharmacokinetics of Transdermal Flunixin Meglumine Following a Single Dose in Marine Toads (Rhinella marina). Vet. Med. Int. 2020, 2020, 8863537. [Google Scholar] [CrossRef] [PubMed]
- Sullivan, K.E.; Fleming, G.; Terrell, S.; Smith, D.; Ridgley, F.; Valdes, E.V. Vitamin A values of wild-caught Cuban tree frogs (Osteopilus septentrionalis) and marine toads (Rhinella marina) in whole body, liver, and serum. J. Zoo Wildl. Med. 2014, 45, 892–895. [Google Scholar] [CrossRef] [PubMed]
- Cerreta, A.J.; Smith, D.C.; Ange-van Heugten, K.D.; Minter, L.J. Comparative nutrient analysis of four species of cockroaches used as food for insectivores by age, species, and sex. Zoo Biol. 2021, 41, 26–33. [Google Scholar] [CrossRef] [PubMed]
- Jayson, S.; Ferguson, A.; Goetz, M.; Routh, A.; Tapley, B.; Harding, L.; Michaels, C.J.; Dawson, J. Comparison of the nutritional content of the captive and wild diets of the critically endangered mountain chicken frog (Leptodactylus fallax) to improve its captive husbandry. Zoo Biol. 2018, 37, 332–346. [Google Scholar] [CrossRef]
- Food and Nutrition Board (FNB); Institute of Medicine. Dietary Fats: Total Fat and Fatty Acids. In Dietary Reference Intakes for Energy, Carbohydrate, Fiber, Fat, Fatty Acids, Cholesterol, Protein, and Amino Acids; National Academies Press: Washington, DC, USA, 2002; pp. 422–541. [Google Scholar]
- Connor, W.E. Importance of n−3 fatty acids in health and disease. Am. J. Clin. Nutr. 2000, 71, 171S–175S. [Google Scholar] [CrossRef]
- Morris, J.G. Do cats need arachidonic acid in the diet for reproduction? J. Anim. Physiol. Anim. Nutr. 2004, 88, 131–137. [Google Scholar] [CrossRef]
- Linus Pauling Institute. Essential Fatty Acids. In Micronutrient Information Center; Jump, D.P., Ed.; Oregon State University: Corvallis, OR, USA, 2022. [Google Scholar]
- Surai, P.F.; Noble, R.C.; Sparks, N.H.; Speake, B.K. Effect of long-term supplementation with arachidonic or docosahexaenoic acids on sperm production in the broiler chicken. J. Reprod. Fertil. 2000, 120, 257–264. [Google Scholar] [CrossRef] [Green Version]
- Samaee, S.; Manteghi, N.; Estevez, A. Zebrafish as a model to screen the potential of fatty acids in reproduction. Zebrafish 2019, 16, 47–64. [Google Scholar] [CrossRef] [Green Version]
- Furuita, H.; Hori, K.; Suzuki Sugita, T.; Yamamoto, T. Effect of n-3 and n-6 fatty acids in broodstock diet on reproduction and fatty acid composition of broodstock and eggs in the Japanese eel. Anguilla Jpn. Aquac. 2007, 267, 55–61. [Google Scholar] [CrossRef]
- Smit, E.N.; Muskiet, F.A.; Boersma, E.R. The possible role of essential fatty acids in the pathophysiology of malnutrition: A review. Prostaglandins Leukot. Essent. Fat. Acids 2004, 71, 241–250. [Google Scholar] [CrossRef]
- Wood, J.; Minter, L.J.; Stoskopf, M.K.; Bibus, D.; Ange, D.; Tollefson, T.; Ange-van Heugten, K. Verification of dried blood spot cards for fatty acid analysis using porcine blood. Vet. Med. Int. 2021, 2021, 6624751. [Google Scholar] [CrossRef]
- Wood, J.; Minter, L.J.; Bibus, D.; Tollefson, T.; Ange-van Heugten, K. Assessment of the effects of storage temperature on fatty acid analysis using dried blood spot cards collected from managed southern white rhinoceroses (Ceratotherium simum simum): Implications for field collection and nutritional care. PeerJ 2022, 10, e12896. [Google Scholar] [CrossRef]
- Childs, C.E.; Romeu-Nadal, M.; Burdge, G.C.; Calder, P.C. Gender differences in the n-3 fatty acid content of tissues. Proc. Nutr. Soc. 2008, 67, 19–27. [Google Scholar] [CrossRef] [Green Version]
- Walker, C.G.; Browning, L.M.; Mander, A.P.; Madden, J.; West, A.L.; Calder, P.C.; Jebb, S.A. Age and sex differences in the incorporation of EPA and DHA into plasma fractions, cells and adipose tissue in humans. Br. J. Nutr. 2014, 111, 679–689. [Google Scholar] [CrossRef] [Green Version]
- Boden, G. Obesity and free fatty acids. Endocrinol. Metab. Clin. North Am. 2008, 37, 635–646. [Google Scholar] [CrossRef] [Green Version]
Unit | Cricket Diet | Dusting Supplement | Gut-Loaded Crickets (Diet 1) | Gut-Loaded and Dusted Crickets (Diet 2) | |
---|---|---|---|---|---|
Moisture | % | 7.7 | 2.2 | 70.5 | 69.0 |
Dry Matter | % | 92.3 | 97.8 | 29.5 | 31.0 |
Crude Protein | % | 20.2 | 20.5 | 67.3 | 63.3 |
Nitrogen Free Extract | % | 39.5 | ND | - | - |
Acid Detergent Fiber | % | 12.4 | ND | 35.2 | 44.2 |
Neutral Detergent Fiber | % | 13.8 | ND | - | - |
Water Soluble Carbohydrates | % | 3.9 | ND | - | - |
Starch | % | 20.7 | ND | - | - |
Crude Fat (ether extract) | % | 3.8 | ND | - | - |
Crude Fat (acid hydrolysis) | % | - | - | 16.4 | 17.0 |
Crude Fiber | % | 10.4 | ND | - | - |
Ash | % | 23.8 | ND | 6.2 | 9.6 |
Ca | % | 8.1 | 22.7 | 0.8 | 2.3 |
P | % | 0.6 | 0.3 | 1.0 | 0.9 |
Ca:P ratio | 1 to | 13.8 | 78.2 | 0.8 | 2.5 |
Mg | % | 0.3 | 0.2 | 0.1 | 0.1 |
K | % | 0.9 | 0.5 | 1.2 | 1.1 |
Na | % | 0.2 | 0.4 | 0.4 | 0.4 |
Cl | % | ND | 0.3 | - | - |
S | % | 0.3 | 0.5 | 0.6 | 0.6 |
Fe | ppm | 297 | 247 | 61 | 73 |
Zn | ppm | 74 | 10 | 184 | 168 |
Cu | ppm | 16 | ND | 21 | 20 |
Mn | ppm | 99 | 55 | 39 | 39 |
Mo | ppm | 1.7 | 1.7 | 1.7 | 1.3 |
Co | ppm | ND | 0.5 | - | - |
Number | Common Name | Diet Item Analyzed | ||
---|---|---|---|---|
Cricket-Dusting Supplement | Cricket Diet | Gut-Loaded Crickets (Diet 1) | ||
Individual Fatty Acids | ||||
12:0 | Lauric acid | 0.47 | 0.00 | 0.00 |
14:0 | Myristic acid | 0.69 | 0.65 | 0.58 |
14:1 | Myristoleic acid | 0.24 | 0.00 | 0.00 |
15:0 | Pentadecylic acid | 0.92 | 0.20 | 0.07 |
15:1 | Pentadecenoic acid | ND | ND | ND |
16:0 | Palmitic acid | 44.60 | 19.52 | 27.17 |
16:1n5 | Myristoleic acid | ND | ND | ND |
16:1n7 | Palmitoleic acid | 10.12 | 1.24 | 1.07 |
17:0 | Margaric acid | ND | ND | ND |
17:1 | Heptadecenoic acid | ND | ND | ND |
18:0 | Stearic acid | 0.74 | 7.08 | 8.78 |
18:1n5 | 13-Octadecenoic acid | ND | ND | ND |
18:1n7 | Vaccenic acid | ND | ND | ND |
18:1n9 | Oleic acid | 1.89 | 29.85 | 21.00 |
18:2n6 | Linoleic acid | 19.59 | 34.24 | 36.26 |
18:3n6 | γ-Linoleic acid | 12.69 | 0.08 | 0.00 |
18:3n3 | α-Linolenic acid | 0.12 | 2.50 | 0.99 |
18:4n3 | Stearidonic acid | 0.21 | 0.04 | 0.05 |
20:0 | Arachidic acid | 0.09 | 0.47 | 0.50 |
20:1n7 | Paullinic acid | ND | ND | ND |
20:1n9 | Gondoic acid | 0.00 | 0.68 | 0.13 |
20:2n6 | Eicosadienoic acid | 0.23 | 0.24 | 0.04 |
20:3n9 | Mead acid | 0.00 | 0.00 | 0.22 |
20:3n6 | Dihomo-γ-Linoleic acid | 0.16 | 0.04 | 0.02 |
20:4n6 | Arachidonic acid | 0.09 | 0.06 | 0.25 |
20:3n3 | Eicosatrienoic acid | 0.08 | 0.00 | 0.00 |
20:4n3 | Eicosatetraenoic acid | 0.25 | 0.00 | 0.00 |
20:5n3 | Eicosapentaenoic acid | 0.32 | 0.18 | 0.39 |
22:0 | Behenic acid | 0.15 | 0.20 | 0.04 |
22:1n9 | Erucic acid | 0.12 | 0.02 | 0.02 |
22:4n6 | Adrenic acid | 0.03 | 0.13 | 0.18 |
22:5n6 | n6 Docosapentaenoic acid | 0.04 | 0.07 | 0.19 |
22:5n3 | n3 Docosapentaenoic acid | 0.06 | 0.11 | 0.08 |
22:6n3 | Docosahexanoic acid | 0.05 | 0.08 | 0.10 |
24:0 | Lignoceric acid | 0.11 | 0.20 | 0.05 |
24:1 | Nervonic acid | 0.00 | 0.01 | 0.13 |
Other | 5.90 | 2.09 | 1.66 | |
Fatty Acid Groupings | ||||
Monoenes | 2.27 | 30.56 | 21.28 | |
Saturates | 47.77 | 28.33 | 37.19 | |
Highly Unsaturated Fatty Acids | 14.12 | 3.32 | 2.50 | |
Poly Unsaturated Fatty Acids | 33.93 | 37.79 | 38.80 | |
n-3 Fatty Acids | 1.09 | 2.92 | 1.63 | |
n-6 Fatty Acids | 32.83 | 34.84 | 36.95 | |
n-9 Fatty Acids | 2.24 | 29.88 | 21.15 | |
n6: n3 Fatty Acid Ratio | 30.15 | 11.92 | 22.72 |
Fatty Acid | Common Name | Sample Location and Testing Method | ||
---|---|---|---|---|
Free-Ranging DBS (n = 10) | Managed Care DBS (n = 12) | Managed Care WBV (n = 12) | ||
Individual Fatty Acids | ||||
12:0 | Lauric acid 12:0 | ND | ND | ND |
14:0 | Myristic acid | 0.9 ± 0.15 | 0.9 ± 0.11 | 0.9 ± 0.11 |
14:1 | Myristoleic acid | 0.1 ± 0.03 | 0.1 ± 0.02 | 0.1 ± 0.02 |
15:0 | Pentadecylic acid | NQ | NQ | 3.6 ± 0.24 |
15:1 | Pentadecenoic acid | 0.2± 0.02 | 0.1 ± 0.02 | 0.1 ± 0.02 |
16:0 | Palmitic acid | 15.3 ± 0.64 | 16.9 ± 0.59 | 16.7 ± 0.59 |
16:1n5 | Myristoleic acid | ND | ND | ND |
16:1n7 | Palmitoleic acid | 2.5 ± 0.53 a | 4.9 ± 0.48 b | 5.3 ± 0.48 b |
17:0 | Margaric acid | 1.0 ± 0.13 a | 0.3 ± 0.12 b | 0.5 ± 0.12 b |
17:1 | Heptadecenoic acid | 0.6 ± 0.09 a | 0.3 ± 0.08 b | 0.2 ± 0.08 b |
18:0 | Stearic acid | 6.3 ± 0.45 | 5.4 ± 0.41 | 5.7 ± 0.41 |
18:1n5 | 13-Octadecenoic acid | ND | ND | ND |
18:1n7 | Vaccenic acid | ND | ND | ND |
18:1n9 | Oleic acid | 27.6 ± 0.93 a | 19.1 ± 0.85 b | 19.4 ± 0.85 b |
18:2n6 | Linoleic acid | 28.2 ± 1.35 a | 46.1 ± 1.23 b | 42.3 ± 1.23 c |
18:3n6 | γ-Linoleic acid | 0.3 ± 0.02 a | 0.05 ± 0.02 b | 0.06 ± 0.02 b |
18:3n3 | α-Linolenic acid | 2.6 ± 0.39 a | 0.9 ± 0.35 b | 0.8 ± 0.35 b |
18:4n3 | Stearidonic acid | 0.1 ± 0.04 | 0.1 ± 0.03 | 0.1 ± 0.03 |
20:0 | Arachidic acid | 0.2 ± 0.02 | 0.2 ± 0.02 | 0.2 ± 0.02 |
20:1n7 | Paullinic acid | 0.3 ± 0.04 a | 0.2 ± 0.03 b | 0.2 ± 0.03 b |
20:1n9 | Gondoic acid | ND | ND | ND |
20:2n6 | Eicosadienoic acid | 0.4 ± 0.02 | 0.3 ± 0.02 | 0.3 ± 0.02 |
20:3n3 | Eicosatrienoic acid | ND | ND | ND |
20:3n6 | Dihomo-γ-Linoleic acid | 0.6 ± 0.04 a | 0.2 ± 0.03 b | 0.2 ± 0.03 b |
20:3n9 | Mead acid | ND | ND | ND |
20:4n6 | Arachidonic acid | 7.1 ± 0.40 a | 1.6 ± 0.37 b | 1.3 ± 0.37 b |
20:4n3 | Eicosatetraenoic acid | 0.0 ± 0.03 a | 0.1 ± 0.03 a | 0.2 ± 0.03 b |
20:5n3 | Eicosapentaenoic acid | 1.0 ± 0.24 | 0.7 ± 0.22 | 0.5 ± 0.22 |
22:0 | Behenic acid | 0.2 ± 0.01 a | 0.1 ± 0.01 b | 0.04 ± 0.01 c |
22:1n9 | Erucic acid | 0.2 ± 0.07 a | 0.1 ± 0.06 a | 0.5 ± 0.06 b |
22:4n6 | Adrenic acid | 0.6 ± 0.07 a | 0.2 ± 0.06 b | 0.2 ± 0.06 b |
22:5n6 | n6 Docosapentaenoic acid | 0.1 ± 0.02 a | 0.0 ± 0.02 b | 0.06± 0.02 b |
22:5n3 | n3 Docosapentaenoic acid | 1.1 ± 0.10 a | 0.5 ± 0.10 b | 0.4 ± 0.10 b |
22:6n3 | Docosahexanoic acid | 0.6 ± 0.14 | 0.3 ± 0.13 | 0.2 ± 0.13 |
24:0 | Lignoceric acid | ND | ND | ND |
24:1 | Nervonic acid | 0.2 ± 0.03 a | 0.1 ± 0.03 b | 0.1 ± 0.03 b |
Fatty Acid Groupings | ||||
Monoenes | 28.6 ± 0.94 a | 19.6 ± 0.86 b | 20.3 ± 0.86 b | |
Saturates | 24.4 ± 0.84 a | 23.9 ± 0.77 a | 27.2 ± 0.77 b | |
Highly Unsaturated Fatty Acids | 11.2 ± 0.73 a | 3.6 ± 0.67 b | 2.9 ± 0.67 b | |
Poly Unsaturated Fatty Acids | 42.8 ± 1.56 a | 51.0 ± 1.42 b | 46.4 ± 1.42 a | |
n-3 Fatty Acids | 5.4 ± 0.69 a | 2.6 ± 0.63 b | 2.1 ± 0.63 b | |
n-6 Fatty Acids | 37.4 ± 1.39 a | 48.4 ± 1.26 b | 44.3 ± 1.26 c | |
n-9 Fatty Acids | 28.1 ± 0.92 a | 19.3 ± 0.84 b | 20.0 ± 0.84 b | |
n-6:n-3 Fatty Acid Ratio | 8.6 ± 1.63 a | 23.1 ± 1.48 b | 22.0 ± 1.48 b |
Fatty Acid | Common Name | Diet Type | |
---|---|---|---|
Gut-loaded Crickets (Diet 1) (n = 6) | Gut-loaded and Dusted Crickets (Diet 2) (n = 6) | ||
Individual Fatty Acids | |||
12:0 | Lauric acid | ND | ND |
14:0 | Myristic acid | 1.0 ± 0.14 | 0.8 ± 0.14 |
14:1 | Myristoleic acid | 0.1 ± 0.04 | 0.1 ± 0.04 |
15:0 | Pentadecylic acid | NQ | NQ |
15:1 | Pentadecenoic acid | 0.1 ± 0.02 | 0.1 ± 0.02 |
16:0 | Palmitic acid | 16.9 ± 0.56 | 17.0 ± 0.56 |
16:1n5 | Myristoleic acid | ND | ND |
16:1n7 | Palmitoleic acid | 5.0 ± 0.73 | 4.9 ± 0.73 |
17:0 | Margaric acid | 0.2 ± 0.13 | 0.4 ± 0.13 |
17:1 | Heptadecenoic acid | 0.2 ± 0.09 | 0.3 ± 0.09 |
18:0 | Stearic acid | 5.2 ± 0.25 | 5.7 ± 0.25 |
18:1n5 | 13-octadecenoic acid | ND | ND |
18:1n7 | Vaccenic acid | ND | ND |
18:1n9 | Oleic acid | 18.3 ± 0.68 | 19.8 ± 0.68 |
18:2n6 | Linoleic acid | 46.7 ± 1.25 | 45.4 ± 1.25 |
18:3n6 | γ-Linoleic acid | 0.04 ± 0.02 | 0.06 ± 0.02 |
18:3n3 | α-Linolenic acid | 0.9 ± 0.04 | 0.9 ± 0.04 |
18:4n3 | Stearidonic acid | 0.2 ± 0.08 | 0.1 ± 0.08 |
20:0 | Arachidic acid | 0.2 ± 0.03 | 0.2 ± 0.03 |
20:1n7 | Paullinic acid | 0.2 ± 0.04 | 0.2 ± 0.04 |
20:1n9 | Gondoic acid | ND | ND |
20:2n6 | Eicosadienoic acid | 0.3 ± 0.03 | 0.3 ± 0.03 |
20:3n3 | Eicosatrienoic acid | ND | ND |
20:3n6 | Dihomo-γ-Linoleic acid | 0.2 ± 0.04 | 0.2 ± 0.04 |
20:3n9 | Mead acid | ND | ND |
20:4n6 | Arachidonic acid | 1.5 ± 0.53 | 1.7 ± 0.53 |
20:4n3 | Eicosatetraenoic acid | 0.06 ± 0.02 | 0.04 ± 0.02 |
20:5n3 | Eicosapentaenoic acid | 1.1 ± 0.42 | 0.4 ± 0.42 |
22:0 | Behenic acid | 0.1 ± 0.01 | 0.1 ± 0.01 |
22:1n9 | Erucic acid | 0.02 ± 0.02 a | 0.09 ± 0.02 b |
22:4n6 | Adrenic acid | 0.1 ± 1.33 | 0.2 ± 1.33 |
22:5n6 | n6 Docosapentaenoic acid | 0.04 ± 0.02 | 0.02 ± 0.02 |
22:5n3 | n3 Docosapentaenoic acid | 0.5 ± 0.10 | 0.5 ± 0.10 |
22:6n3 | Docosahexanoic acid | 0.5 ± 0.28 | 0.1 ± 0.28 |
24:0 | Lignoceric acid | ND | ND |
24:1 | Nervonic acid | 0.02 ± 0.02 a | 0.08 ± 0.02 b |
Fatty Acid Groupings | |||
Monoenes | 18.8 ± 0.68 | 20.4 ± 0.68 | |
Saturates | 23.6 ± 0.53 | 24.2 ± 0.53 | |
Highly Unsaturated Fatty Acids | 4.0 ± 1.02 | 3.2 ± 1.02 | |
Poly Unsaturated Fatty Acids | 52.1 ± 1.32 | 49.9 ± 1.32 | |
n-3 Fatty Acids | 3.2 ± 0.85 | 2.0 ± 0.85 | |
n-6 Fatty Acids | 48.9 ± 1.30 | 47.9 ± 1.30 | |
n-9 Fatty Acids | 18.5 ± 0.67 | 20.1 ± 0.67 | |
n-6: n-3 Fatty Acid Ratio | 21.5 ± 2.60 | 24.7 ± 2.60 |
Fatty Acid | Common Name | Female (n = 5) | Male (n = 5) |
---|---|---|---|
Individual Fatty Acids | |||
12:0 | Lauric acid | ND | ND |
14:0 | Myristic acid | 1.0 ± 0.22 | 0.8 ± 0.22 |
14:1 | Myristoleic acid | 0.1 ± 0.05 | 0.1 ± 0.05 |
15:0 | Pentadecylic acid | NQ | NQ |
15:1 | Pentadecenoic acid | 0.2 ± 0.21 | 0.1 ± 0.21 |
16:0 | Palmitic acid | 16.1 ± 0.49 a | 14.4 ± 0.49 b |
16:1n5 | Myristoleic acid | ND | ND |
16:1n7 | Palmitoleic acid | 2.6 ± 0.31 | 2.5 ± 0.31 |
17:0 | Margaric acid | 1.3 ± 0.20 | 0.7 ± 0.20 |
17:1 | Heptadecenoic acid | 0.9 ± 0.14 a | 0.3 ± 0.14 b |
18:0 | Stearic acid | 6.2 ± 0.47 | 6.3 ± 0.47 |
18:1n5 | 13-octadecenoic acid | ND | ND |
18:1n7 | Vaccenic acid | ND | ND |
18:1n9 | Oleic acid | 27.4 ± 2.11 | 27.9 ± 2.11 |
18:2n6 | Linoleic acid | 28.8 ± 1.76 | 27.7 ± 1.76 |
18:3n6 | γ-Linoleic acid | 0.3 ± 0.06 | 0.3 ± 0.06 |
18:3n3 | α-Linolenic acid | 1.5 ± 0.92 | 3.7 ± 0.92 |
18:4n3 | Stearidonic acid | 0.1 ± 0.22 | 0.1 ± 0.22 |
20:0 | Arachidic acid | 0.2 ± 0.04 | 0.3 ± 0.04 |
20:1n7 | Paullinic acid | 0.35 ± 0.08 | 0.4 ± 0.08 |
20:1n9 | Gondoic acid | ND | ND |
20:2n6 | Eicosadienoic acid | 0.4 ± 0.02 a | 0.3 ± 0.02 b |
20:3n3 | Eicosatrienoic acid | 0.02 ± 0.01 | 0.04 ± 0.008 |
20:3n6 | Dihomo-γ-Linoleic acid | 0.5 ± 0.06 a | 0.7 ± 0.06 b |
20:3n9 | Mead acid | ND | ND |
20:4n6 | Arachidonic acid | 7.0 ± 0.85 | 7.2 ± 0.85 |
20:4n3 | Eicosatetraenoic acid | 0.01 ± 0.01 | 0.02 ± 0.01 |
20:5n3 | Eicosapentaenoic acid | 0.5 ± 0.27 a | 1.5 ± 0.27 b |
22:0 | Behenic acid | 0.2 ± 0.02 | 0.2 ± 0.02 |
22:1n9 | Erucic acid | 0.2 ± 0.05 | 0.1 ± 0.05 |
22:4n6 | Adrenic acid | 0.6 ± 0.11 | 0.6 ± 0.11 |
22:5n6 | n6 Docosapentaenoic acid | 0.1 ± 0.05 | 0.1 ± 0.05 |
22:5n3 | n3 Docosapentaenoic acid | 0.8 ± 0.18 a | 1.4 ± 0.18 b |
22:6n3 | Docosahexanoic acid | 0.4 ±0.11 a | 0.8 ± 0.11 b |
24:0 | Lignoceric acid | 0.1 ± 0.02 | 0.08 ± 0.02 |
24:1 | Nervonic acid | 0.2 ± 0.06 | 0.3 ± 0.06 |
Fatty Acid Groupings | |||
Monoenes | 28.4 ± 2.14 | 28.9 ± 2.14 | |
Saturates | 25.6 ± 0.62 a | 23.1 ± 0.62 b | |
Highly Unsaturated Fatty Acids | 10.1 ± 1.27 | 12.4 ± 1.27 | |
Poly Unsaturated Fatty Acids | 41.1 ± 2.61 | 44.5 ± 2.61 | |
n-3 Fatty Acids | 3.3 ± 1.17 a | 7.5 ± 1.17 b | |
n-6 Fatty Acids | 37.8 ± 1.92 | 37.0 ± 1.92 | |
n-9 Fatty Acids | 27.9 ± 2.10 | 28.4 ± 2.10 | |
n-6: n-3 Fatty Acid Ratio | 11.6 ± 0.625 a | 5.5 ± 0.625 b |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Witt, M.L.; Minter, L.J.; Tollefson, T.N.; Ridgley, F.; Treiber, K.; Smith, D.; Bibus, D.; Scott, H.; Ange-van Heugten, K. Analysis of Circulating Fatty Acid Profiles in Free-Ranging and Managed Care Marine Toads (Rhinella marina) with a Comparison of Whole-Blood Vial and Whole-Blood Dried Blood Spot Card Analyses. J. Zool. Bot. Gard. 2022, 3, 300-315. https://doi.org/10.3390/jzbg3030024
Witt ML, Minter LJ, Tollefson TN, Ridgley F, Treiber K, Smith D, Bibus D, Scott H, Ange-van Heugten K. Analysis of Circulating Fatty Acid Profiles in Free-Ranging and Managed Care Marine Toads (Rhinella marina) with a Comparison of Whole-Blood Vial and Whole-Blood Dried Blood Spot Card Analyses. Journal of Zoological and Botanical Gardens. 2022; 3(3):300-315. https://doi.org/10.3390/jzbg3030024
Chicago/Turabian StyleWitt, Melissa L., Larry J. Minter, Troy N. Tollefson, Frank Ridgley, Kimberly Treiber, Dustin Smith, Doug Bibus, Heather Scott, and Kimberly Ange-van Heugten. 2022. "Analysis of Circulating Fatty Acid Profiles in Free-Ranging and Managed Care Marine Toads (Rhinella marina) with a Comparison of Whole-Blood Vial and Whole-Blood Dried Blood Spot Card Analyses" Journal of Zoological and Botanical Gardens 3, no. 3: 300-315. https://doi.org/10.3390/jzbg3030024