Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7040043

Authors: Alejandra Vieyra-Ramírez Lisa Moran Cara T. Daly Zeta Dooly Thomais Kakouli-Duarte

Gardens provide several ecosystem services, including functioning as a habitat for diverse taxa and promoting users’ well-being. Soil biodiversity is considered an important factor in the capacity of the soil to provide ecosystem services. Nematodes are widely used as environmental indicators based on their key roles in the soil food web. Nematode and plant communities were studied across five different areas in a garden in Ireland as part of an assessment of soil health, as well as to explore their distribution patterns inside the garden. The aims of the study were to contribute to the exploration of soil biodiversity and soil health in built environments, and to study nematode communities in different plant assemblages. Using nematode morphology, a total of 33 terrestrial nematode families were identified across the garden, indicating similar levels of soil ecosystem enrichment and food web structure among different plant assemblages. A total of 25 plant families were identified in the five areas, revealing distinct plant communities inside the gardens. The study was limited to a single sampling event and the taxonomic identification of nematode and plants at the family level. However, the results highlight the use of nematodes as environmental indicators for soil health assessments in gardens, and provided baseline information that could potentially inform on soil biodiversity and plant management strategies.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7040042

Authors: Matheus Pissarelli Cristiano Schetini de Azevedo Selma Maria de Almeida-Santos Bruno Martins da Costa Batista Gabriel Polidoro Cristiane Schilbach Pizzutto

Intensive snake husbandry requires evidence-based approaches to environmental management and animal welfare. This study characterized substrate use in six juvenile South American rattlesnakes (Crotalus durissus) by comparing a familiar substrate (corrugated cardboard) with four novel substrates (Sand, Gravel, Dry leaves, and Sphagnum) during diurnal and nocturnal periods. Behaviour was continuously recorded over 288 h, and the proportion of time spent in five body-contact categories, ranging from 100% to 0% contact with the novel substrate, was analyzed using Beta Generalized Linear Mixed Models (GLMMs). The familiar cardboard area (category E; 0% contact with the novel substrate) accounted for the largest proportion of total observation time. However, a complementary analysis comparing No contact (category E) with Any contact (categories A–D combined) revealed no significant overall difference between contact categories. Instead, a significant Contact × Substrate interaction indicated substrate-specific patterns of contact, with Any contact accounting for a greater proportion of time on Sand and Sphagnum, whereas No contact was more prevalent on Gravel and Dry leaves. No significant effect of experimental period was detected. These findings indicate that juvenile rattlesnakes displayed different patterns of contact with novel substrates while continuing to use the familiar area within the enclosure, suggesting that substrate characteristics and familiarity may both contribute to patterns of substrate use. From a husbandry perspective, these results highlight the value of considering familiarity alongside the physical properties of substrates when designing environmental complexity for captive rattlesnakes. Behavioural assessment of substrate contact may therefore provide a useful approach for characterizing how juvenile snakes interact with different environmental substrates and informing husbandry practices.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030041

Authors: Tatiane Cezário dos Santos Roberta Lane de Oliveira Silva Adriana Mayumi Yano-Melo Natoniel Franklin de Melo

Ex situ germplasm collections are essential for conserving plant genetic resources, but their effectiveness depends on how well they represent the genetic and environmental diversity of natural populations. Passiflora cincinnata Mast., an endemic wild passion fruit species from the Brazilian Caatinga, is an important source of adaptive traits for conservation and breeding under semi-arid conditions. This study evaluated the genetic diversity and environmental diversity represented by 55 accessions maintained in the Active Passion Fruit Germplasm Bank of Embrapa Semiárido. Genetic diversity was assessed using eight ISSR primers, percentage of polymorphic loci, Shannon’s information index, expected heterozygosity (He), UPGMA clustering, principal coordinate analysis (PCoA), analysis of molecular variance (AMOVA), and Mantel tests. Environmental characterization was based on WorldClim bioclimatic variables, principal component analysis (PCA), k-means clustering, and PERMANOVA. Substantial genetic diversity was detected across the collection, with an overall expected heterozygosity (He) of 0.444 and 100% polymorphic loci. Most genetic variation occurred within biogeographical domains (97.1%), whereas differentiation among domains was low (PhiPT = 0.029; p = 0.124). Five distinct climatic groups were identified, reflecting contrasting temperature and precipitation regimes across the Caatinga. The integration of molecular and environmental analyses showed that the collection encompasses substantial genetic diversity and broad environmental variation, providing complementary information for conservation planning, germplasm management, and climate-resilient passion fruit breeding.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030040

Authors: Areli González-Cortés M. Humberto Reyes-Valdés Laura Raquel Luna-García Rosalinda Mendoza-Villarreal Miguel Ángel Pérez-Rodríguez Nadia Landero-Valenzuela Valentín Robledo-Torres

Species of the genus Opuntia exhibit considerable variation in chromosome number and ploidy level, making cytogenetic information essential for studies on evolution, taxonomy, and genetic improvement. However, cytogenetic information on several species of this genus remains limited. Therefore, the objectives of this study were to determine chromosome numbers and ploidy levels in seven Opuntia species and to characterize the karyotype of Opuntia microdasys as a representative diploid species. A total of 69 accessions representing seven species were collected from wild and cultivated populations in southeastern Coahuila, Mexico. Somatic cells obtained from root apices developed from cladodes were used to analyze chromosome numbers. Cytological preparations included determination of mitotic activity, pretreatment with p-dichlorobenzene, enzymatic digestion with pectolyase and cellulase, and acetocarmine staining, followed by chromosome counting and karyotype analysis of O. microdasys. The highest mitotic activity was recorded between 10:00 and 11:00 h. Chromosome counts revealed diploid (2n = 22), tetraploid (2n = 44), hexaploid (2n = 66), and octoploid (2n = 88) cytotypes. Notably, O. engelmannii exhibited intraspecific cytotypic variation, with both hexaploid and octoploid populations. The karyotype of O. microdasys was highly symmetrical, with a karyotype formula of 10m + 1sm, small chromosomes, and low asymmetry indices (A1 = 0.15; A2 = 0.106). These findings reveal substantial ploidy diversity among the seven Opuntia species analyzed and provide cytogenetic information relevant to the characterization, management, and conservation of Opuntia genetic resources, including their potential use in ex situ conservation and botanical collections.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030039

Authors: Frej Gammelgård Silje Marquardsen Lund Jonas Nielsen Thea Loumand Faddersbøll Trine Hammer Jensen Sussie Pagh Kirstin Anderson Hansen Flemming Nielsen Cino Pertoldi

Behavioral observation is central to animal welfare assessment in zoological institutions, but manual observation is time-consuming and difficult to apply continuously. We developed and applied a LabGym-based workflow for automated behavioral monitoring of captive lions (Panthera leo). Development footage from three Danish zoological institutions was used to train a lion detector and a seven-class behavior categorizer; video collected on 22–28 December 2025 was temporally held out from model development and used for applied validation. Detector performance was moderate (bounding-box AP 47.1%; segmentation AP 41.6%), while the internal categorizer validation reached 0.62 accuracy and weighted F1. In the held-out validation, 52.6% of manually annotated lion-time received a retained pipeline prediction. Fine-scale end-to-end accuracy was 0.231 and increased to 0.440 when calculated only on covered lion-time. Grouping behaviors as active, inactive/maintenance, and pacing-like locomotion increased these values to 0.317 and 0.602, respectively. Coverage varied substantially among institutions (33.8–59.8%) and was particularly low for cubs and nighttime footage. Although model macro-F1 exceeded simple majority-class baselines, end-to-end accuracy did not. The workflow demonstrates the potential of computer-vision approaches for scalable behavioral quantification and future welfare assessment in zoological settings, while highlighting the importance of accounting for prediction coverage and classification performance when interpreting automated behavioral data.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030038

Authors: Ruofan Yu Hui Fu

Against the backdrop of the coordinated development of biodiversity conservation, the preservation of ethnic cultures, and public environmental education, the traditional environmental education model in national forest parks—which primarily focuses on showcasing natural landscapes and one-way knowledge dissemination—struggles to fully address the practical needs of dynamic ecosystem conservation and the sustainable preservation of local cultures. This paper focuses on the objectives, content, and implementation mechanisms of environmental education in national forest parks that integrate ethnic cultures. It analyzes the intrinsic connections among digital technology, community collaboration, traditional ecological knowledge, and hands-on environmental education and constructs a “Hub” model centered on the integration of digital knowledge and collaborative governance. This model employs digital technology, community collaboration, and hands-on environmental education as its three primary implementation pathways. Through a cyclical mechanism of “knowledge collection—digital integration—community validation—educational transformation—ecological practice—feedback optimization,” it connects ethnic cultural knowledge, modern ecological science, and public participatory conservation actions. In this model, digital technology primarily serves to record, integrate, and disseminate ecological data and ethnic cultural archives; community collaboration emphasizes the leading role of ethnic communities in knowledge interpretation, curriculum design, ecological practices, and conservation decision-making; and environmental field education promotes the transformation of learners from ecological awareness and cultural identity toward environmental responsibility and conservation behavior through nature observation, cultural experiences, ecological field studies, and community participation. This paper argues that this model can drive the transformation of environmental education in national forest parks from static displays and one-way communication toward knowledge co-creation, scenario-based learning, and adaptive governance, thereby providing a theoretically grounded framework and practical pathway—tailored to local contexts—for achieving the coordinated development of biodiversity conservation, the preservation of ethnic cultural heritage, and the enhancement of public ecological literacy.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030037

Authors: Lisa A. Macdonald Rasmus Praefke Sabine Frühwirth Angela S. Stoeger

The northern rockhopper penguin (Eudyptes moseleyi) is an endangered species undergoing continued population decline, making ex situ conservation and evidence-based breeding management increasingly important. Yet, behavioural data from ex situ populations remain limited. We provide the first systematic assessment of selected breeding-related behaviours in 13 pairs at Vienna Zoo during the 2024 breeding season using focal video observations. We quantified sex-specific investment in incubation, brooding and nest building, recorded agonistic interactions and nest-material competition, and derived composite indices of pair bonding, coordination, conflict exposure, and aggression initiation. Females contributed significantly more to incubation, whereas males invested more time in nest building, initiated more contact aggression and stole nest material more frequently. Males also received more non-contact aggression but fewer contact aggression events. Higher pair coordination was associated with lower conflict exposure, but the behavioural indices did not reliably predict reproductive success. Birth-origin composition (zoo versus wild-born) was associated with breeding outcome, with mixed-origin pairs showing lower breeding success than same-origin pairs. This exploratory association should be interpreted cautiously given the small sample size and possible confounding by demographic or pair-history characteristics. Our findings identify complementary sex-specific roles, nest-material competition and pair-level coordination as components of ex situ breeding behaviour.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030036

Authors: Alex Sargsyan

Human–animal interactions have been associated with numerous benefits for human wellbeing, yet their potential contributions to conservation have received comparatively little theoretical attention. The biophilia hypothesis proposes that humans possess an innate tendency to affiliate with living organisms and provides a compelling theoretical foundation for examining how meaningful interactions with animals may influence both personal and environmental outcomes. The purpose of this paper is to propose a deductively derived conceptual framework describing how meaningful interactions with domesticated waterfowl may contribute simultaneously to human wellbeing and wetland conservation. Interpreted through the biophilia hypothesis, the proposed framework describes two complementary pathways. The therapeutic pathway suggests that meaningful human–waterfowl interactions foster emotional connection with living organisms, contributing to human wellbeing. The conservation pathway proposes that emotional connection may stimulate curiosity and learning about waterfowl and the wetland ecosystems they naturally represent, fostering appreciation for wetlands, conservation awareness, and stewardship. Central to the framework is the proposition that domesticated waterfowl retain a recognizable ecological identity despite domestication, providing a conceptual bridge between meaningful human–animal interactions and the ecosystems these animals naturally represent. The proposed framework integrates concepts from biophilia, human–animal interaction research, conservation education, and conservation psychology while generating empirically testable hypotheses for future research. More broadly, it suggests that meaningful interactions with domesticated waterfowl may represent one pathway through which human wellbeing and wetland conservation can be advanced simultaneously. Although broadly applicable across multiple settings, the proposed framework has particular applicability to zoological institutions, where meaningful observation of ambassador waterfowl, interpretation, and conservation education may provide opportunities to simultaneously promote visitor wellbeing and strengthen conservation awareness and stewardship.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030035

Authors: Xueyang Fan Ruiqing Lv James Edward Ayala

Translocations of endangered species are a common method of conserving wildlife; however, the release of dietary specialists pose unique challenges for conservationists. In preparation for future releases of the red panda (Ailurus fulgens styani), a bamboo specialist, a proof-of concept test was performed using the ‘Human-Assisted Soft Release’ (HASR) method previously used with giant pandas. Two red pandas (one male, one female) were trained using positive reinforcement to recall to a researcher and voluntary placement of GPS collars as well as physical examinations. Afterwards, to simulate a translocation, the individuals were ‘mock-released’ into a large public access red panda enclosure. Following the ‘mock-release’, the pandas were monitored for four weeks via GPS collars mounted with accelerometers as well as the HASR method. The protocol proved operationally successful: across eight scheduled sessions, both individuals met all four predefined criteria in every session attended (8/8 and 7/7, the latter shortened by injury), permitting recall, collar changes, body examinations and weighing inside a public enclosure without anesthesia or physical restraint, and allowing an injury to be detected from collar data and treated the following morning. Alongside this operational record, activity and space use were monitored descriptively. The odds of being active in the first week rose to 1.9 and 1.6 times each individual’s own pre-release baseline (+13.7 and +8.3 percentage points; p < 0.001) before returning to baseline across the month, and the two individuals differed in the reshaping of their daily rhythm and in their use of space. With the limitation of two individuals and an overlap with the breeding season, the behavioral observations reported here are descriptive and hypothesis-generating rather than hypothesis-confirming and require replication. The operational findings nonetheless demonstrate that the HASR method is technically feasible with red pandas and provides a basis for the pre-release preparation of this endangered species.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030034

Authors: Emily C. Lynch Joseph W. Hinton Jennifer B. Nagashima Curtis Malott Natalie J. Johnson Emily Haering Robin Petersen Allison Johnson Julie K. Young

For species extirpated from the wild, ex situ breeding programs are essential for providing the individuals necessary for re-establishing wild populations through reintroductions. However, repeated exposure to humans under managed care may reduce wariness and alter behavioral responses in ways that are maladaptive after release. Although some pre-release behaviors reflect natural responses essential for survival and reproduction, others may result from habituation to humans and increase the risk of conflict, recapture, or mortality following translocation. These risks underscore the need for behavioral assessments to help identify appropriate candidates for release. However, because the assessments themselves require some exposure to humans, pilot studies are needed to determine whether the methods are feasible and reliably capture meaningful individual variation. Here, a standardized behavioral assay was tested to quantify tolerance of ex situ red wolves (Canis rufus) to humans. A pilot study was conducted by staff at the North Carolina Zoo in which repeated observations on 23 red wolves were used during routine husbandry activities to measure three commonly used indices of human tolerance: flight initiation distance, behavioral response to human entry/approach to enclosures, and willingness to consume prey in the presence of humans. Using a multivariate framework, two distinct profiles, human-avoidant and human-tolerant, were identified, in which older red wolves were more likely to exhibit human-tolerant profiles, suggesting an age-related association, consistent with cumulative habituation. These results demonstrate that behavioral profiles can be developed from observations made during routine husbandry activities, requiring no additional handling or invasive testing protocols. This study outlines how the tool can be used for release-candidate screening and adapted for use across facilities participating in the red wolf ex situ breeding program.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030033

Authors: Ayaz Ahmad Mian Muhammad Ahmed Muhammad Saud Khan Syeda Maira Hamid Muqaddas Muhammad Shahbaz Gul Sumbal Ayaz Muzmil Iqbal Muhammad Asim Muhammad Masood Nabi Shuihong Chen Muhammad Bilal Khan

Botanical gardens are highly heterogeneous plant systems characterized by high taxonomic diversity, with numerous plant taxa represented within confined areas, and complex ecological interactions that create unique challenges for pest management. Conventional IPM strategies, designed for simplified agroecosystems, often fail to address complex pest pressures in curated environments. Microbial biostimulants have emerged as promising components of sustainable IPM strategies by enhancing plant defense responses, improving stress resilience, and reducing reliance on chemical inputs. This review synthesizes current knowledge on microbial biostimulants, including plant growth-promoting rhizobacteria, arbuscular mycorrhizal fungi and endophytic microorganisms, in modulating plant defense against insect herbivores. These beneficial microbes enhance plant resistance through multiple mechanisms. They activate induced systemic resistance and modulate key phytohormones, including jasmonic acid, salicylic acid, and ethylene. Additionally, they regulate calcium-dependent and reactive oxygen species-mediated defenses. Microbially induced changes in plant secondary metabolites and volatile organic compounds further influence herbivore behavior and trophic interactions. Emphasis is placed on integrating microbial biostimulants into IPM frameworks tailored to botanical gardens. This highlights compatibility with biological control agents and reduced reliance on synthetic pesticides. Despite promising advances, challenges remain, including context-dependent efficacy, host specificity and limited long-term validation. Overall, microbial biostimulants offer a promising tool for enhancing IPM in biodiversity-rich botanical gardens, although further long-term validation is needed to fully assess their sustainability and effectiveness.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030032

Authors: Claire Reddy Neville Pillay

Olfactory enrichment is recommended for captive felids, but systematic evidence on practical implementation in multiple species and institutions is limited. We evaluated a simple, keeper-safe scent diffuser delivering spice-based odours to captive lions (Panthera leo), tigers (Panthera tigris), jaguars (Panthera onca), leopards (Panthera pardus), cheetahs (Acinonyx jubatus) and pumas (Puma concolor) at two zoological institutions in Johannesburg, South Africa. Behaviour was recorded during baseline and scent-exposure conditions in 23 individuals. Behavioural diversity (number of distinct behaviours per 15-min session) was the primary outcome. Engagement duration, latency to approach and individual response consistency were also quantified. Animals were exposed to a compound spice blend and four component spices (coriander, paprika, black pepper and garlic) and subsequently to decreasing concentrations of their most preferred scent. Behavioural diversity increased significantly during scent exposure at both institutions (1.9 times greater at Lory Park and 1.8 times greater at Johannesburg Zoo), with large effect sizes at both sites (Cohen’s d ≥ 1.2). Spice type did not significantly affect behavioural diversity, but individual animals showed consistent engagement preferences, approach latencies and response profiles during repeated exposures. Full odour concentrations showed significantly longer engagement durations than low concentrations at both institutions (approximately twofold at Lory Park and threefold at Johannesburg Zoo), but behavioural diversity did not differ significantly between concentration levels. No significant species differences were detected, indicating that assessment by individuals is more informative than species level predictions for enrichment planning. Spice-based diffusers provide a practical, low-cost enrichment tool suitable for multi-institutional implementation. Moreover, enrichment programmes should be evaluated and tailored at the individual animal level.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030031

Authors: Leonardo D. Fernández Carlos Zamora-Manzur Italo F. Treviño-Zevallos Jaime Herrera

Lardizabala biternata is an endemic vine and the sole species of the monotypic genus Lardizabala, distributed across central–southern Chile and producing edible fruits traditionally harvested from wild populations. Despite its cultural relevance, potential agronomic value, and evolutionary singularity, its conservation status remains poorly understood because of incomplete distributional knowledge and historical nomenclatural inconsistencies. Here, we conducted a preliminary record-based spatial conservation assessment of L. biternata using a taxonomically and geographically curated occurrence database integrated with Kernel Density Estimation (KDE), Extent of Occurrence (EOO), Area of Occupancy (AOO), overlap analyses with Chile’s protected-area network (SNASPE) and the Chilean Winter Rainfall–Valdivian Forests biodiversity hotspot, and ecological representativeness assessments based on mapped vegetation-class units. Our analyses revealed substantial spatial and ecological conservation gaps. Most documented occurrence records were located outside protected areas, while KDE-derived occurrence-record density patterns were concentrated within the biodiversity hotspot, a region characterized by high endemism and intense anthropogenic pressure. Although EOO indicated a relatively broad geographic extent, AOO indicated restricted occupancy based on documented records. In addition, several mapped vegetation-class units intersecting the 95% KDE-derived occurrence-record density area lacked formal conservation coverage entirely. Together, these findings suggest that L. biternata may warrant greater conservation attention than previously recognized. This study provides the first integrated record-based spatial conservation assessment of the species and establishes a baseline framework for future conservation evaluations, ecological studies, and ex situ conservation strategies.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030030

Authors: Mohamed M. Hassona Abdulrahman A. Al-Hammadi Fatima S. Al-Khulaifi

The Arab world has produced almost no published data on public awareness of botanic gardens, despite having 53 institutions registered with Botanic Gardens Conservation International (BGCI) across 16 of the 22 League of Arab States (LAS) member states. Between October 2024 and December 2025, we administered an 18-item bilingual (Arabic and English) online questionnaire across the 22 LAS member states via paid Facebook advertising, complemented by an institutional inventory from BGCI GardenSearch and an Arabic–French–English triangulation that identified 16 additional non-BGCI operational institutions. Pearson and Spearman correlations between BGCI count and 6 awareness indicators were computed for the 13 countries with n ≥ 10. The survey returned 613 valid responses from all 22 nationalities. Among respondents in this Facebook-recruited sample, 87.4% had heard of botanic gardens (95% CI 84.6–89.8) and 98.9% considered them important for conservation, although only 63.5% reported familiarity with their conservation role. Local awareness ranged from 30% (Palestine) to 100% (Sudan, Lebanon). No BGCI–awareness correlation reached p < 0.05; Q5 recognition reached marginal significance (Spearman ρ = 0.525, p = 0.065). We did not detect a statistically significant association between BGCI institutional count and country-level awareness indicators. Because this analysis rests on only 13 countries, the absence of a significant correlation should not be read as evidence that no association exists. The 35-point gap between affirmation of conservation importance and functional familiarity marks the most actionable target for science communication. We disclose the limitations of Facebook-based recruitment and gender imbalance.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030029

Authors: Luis Eduardo López-Vargas Diego Macías-Suárez Diego J. Macías-Pinto Laura I. López-Botina

University campuses with native vegetation function as living botanical gardens, biodiversity laboratories and curated plant collections, sharing core missions with formal botanical institutions: floristic inventory, in situ and ex situ plant conservation, scientific interpretation, environmental education and biodiversity stewardship. Against this backdrop, limited plant awareness—the tendency to overlook and undervalue plants in one’s surroundings—remains a structural challenge in botany teaching. This article proposes the floristic inventory of the Tulcán campus (Universidad del Cauca, Popayán, Colombia) as the core of a pedagogical strategy that operates the campus as a non-formal botanical educational institution. A sequential explanatory mixed-methods design was applied: (1) ecological inventory across 25 systematic field trips during 2025; (2) educational design with faculty validation; and (3) a pilot with 142 undergraduate students of Biology and Environmental Engineering. We recorded 160 species in 65 families (83% native, including endemic and threatened taxa); designed a four-component strategy (cooperative field research, biocultural connection, applied plant ecology, interpretive trails); and observed a descriptive performance gradient favouring field-immersive courses, together with qualitative patterns consistent with limited plant awareness. The Tulcán campus can be intentionally managed as a living botanical garden delivering inventory, conservation, interpretation and education functions.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030028

Authors: Silvia Beatriz Bürger Tinelli Regiane Quesada Bertão Joyner David Anaya Miranda Ezilda Jacomassi Zilda Cristiani Gazim Odair Alberton Arquimedes Gasparotto Junior

The Ilha Grande National Park region (Brazil) holds one of the most diverse traditional knowledge repositories among Atlantic Forest conservation areas. This study describes the current composition of the medicinal plant knowledge, its taxonomic breadth, and its relevance for conservation. In 2026, this inventory documented 47 species from 33 botanical families cited across a sample of 4 remaining traditional healers. About 27.7% of these specimens are wild/native to nature and 72.3% are cultivated, reflecting both historic management and the residents’ long tradition in household plant acclimatization. The collection includes 25 species of herbs and 11 species of trees, as well as 9 shrubs and 2 climbers. Quantitative analysis at the category level revealed that gastrointestinal disorders had the highest consensus index (ICF = 0.375) after interviews, taxonomic revision, data grouping, and standardizing nomenclature based on the British National Formulary. The results reveal a severe risk of information erosion and a limited representation of younger generations among practitioners, underscoring the need for targeted safeguarding. Strengthening national partnerships, developing cooperative cultivation networks, and improving scientific validation records are proposed as key strategies to enhance the biocultural preservation value of this traditional heritage. Overall, the Ilha Grande healer knowledge serves as an important scientific, educational, and pharmacological resource and plays a central role in advancing the preservation of Brazil’s medicinal plant diversity.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030027

Authors: Spartaco Gippoliti Dario Fraschetti

Antelopes are a crucial part of Western fascination with African ecosystems but are generally well behind other charismatic species. The Nile lechwe Kobus megaceros is a threatened antelope endemic to the Sudd Swamps in Southern Sudan and to neighbouring Ethiopia. It is currently managed as an ex-situ population by both the AZA (Association of Zoos and Aquaria) and the EAZA (European Association of Zoos and Aquaria). Currently, more than thirty European institutions keep the species, while in the 1990s only a handful of European zoos kept it. The foundations of this success story lay partially on the initiatives carried out in the last decades of the 20th century by the staff of the Giardino Zoologico di Roma (now Bioparco), where the species had been kept since 1959 and where in the 1990s one of the then largest European breeding groups of this species was formed. This paper aims to gather all the data, both from published and unpublished sources, on the management and development of this species in the Giardino Zoologico di Roma in the second half of the twentieth century, and to highlight the research, education, and conservation initiatives started by ungulate curator Elisabetta Falchetti. The history of post-World War II Nile lechwe populations in zoos is also discussed. The ex-situ initiatives on the Nile lechwe are also discussed in light of its potential role as a flagship species for the conservation of the wetland ecosystems of South Sudan, which are often overlooked in the education messages conveyed by zoos. This case study illustrates how ex-situ populations may integrate a scientific perspective with a broader cultural and environmental vision so that zoological gardens enhance their conservation message behind the usual flagship species.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030026

Authors: Hugo David Gallardo-Sánchez Ricardo Quirino-Olvera Jorge Armando Verduzco-Martínez Omar Horacio Sol-Torres Fátima Yedith Camacho-Sánchez Miguel Angel Reyes-López

Butterworts (Pinguicula spp.) are carnivorous plants often restricted to small, isolated populations in mountainous microclimates, making them vulnerable to inbreeding and demographic decline. In Mexico, the global hotspot for Pinguicula diversity (>50 species, 90% endemic), conservation is limited by scarce data on population biology, demography, and vulnerability. This study provides the first population size estimate for the endemic P. gracilis and evaluates how patch and population size influence biological fitness components across three localities in northeastern Mexico. Using a multi-component fitness framework and statistical models, we found that smaller populations and patches exhibit significantly reduced reproductive fitness, particularly in fruit set and seed quality. Patch size showed strong positive correlations with fruit production (r = 0.45–0.53), while isolation negatively affected seed output (r = −0.48). These results highlight the role of landscape structure in shaping fitness components, with patch size influencing reproductive effort and connectivity determining reproductive success. Our findings provide the first germplasm and population baseline for P. gracilis and a framework to identify vulnerable populations, prioritize conservation actions, and guide restoration strategies.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7030025

Authors: Kevin Silveira Squarelli Cristiane Schilbach Pizzutto Rodrigo Hidalgo Friciello Teixeira

Managing tigers (Panthera tigris) under human care poses significant risks, particularly with individuals exhibiting aggressive behaviors. Although operant conditioning is widely recommended, structured protocols for reducing aggression in large carnivores remain scarce in the literature. This study investigated the efficacy of a constructional approach, using negative reinforcement as a methodological bridge to positive reinforcement in three adult tigers with a history of generalized aggression. The intervention was based on functional distance applied through negative reinforcement by the trainer’s withdrawal as a consequence of the desired behavior, where the researcher’s presence acted as a functional stimulus and strategic retreat was contingent upon micro-behaviors of calmness. This methodology allowed the animals to exert control over the social interaction, facilitating a systematic transition to positive reinforcement and strengthening voluntary approximation. The results indicated a significant reduction in agonistic responses and a rapid transition toward cooperative behaviors. This work provides detailed evidence on how the constructional approach can overcome food refusal and establish a secure training foundation. We conclude that the protocol offers a replicable methodological framework for managing aggression in wild animals initially resistant to traditional methods, promoting safety and welfare through structured behavioral interactions.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020024

Authors: Paige Klingner Bridget Cooper-Rogers Eduardo J. Fernandez

Otters spend a considerable amount of time vocalizing within zoos and other captive settings. Although associations between these vocalizations and social activity have been investigated prior, it is not always clear how this is reflected in interactions with people under typical zoo conditions, including interactions with zoo visitors and staff. In addition, the implications for zoo welfare and their relation to vocalizing are also not clear, warranting greater investigation to identify potential future welfare metrics for otter species. We examined zoo Asian small-clawed otter vocalizations and general activity in the presence of people, including both visitors and staff. Our focus was on measuring vocalizations as a binomial response (yes/no) in relation to two variables: (a) people, and (b) time. In addition, we also measured differences in vocalizations during sessions, when visitors were present with or without staff. Finally, we measured general otter activity during sessions when 50% or more of the observation intervals had people present (More People), as opposed to sessions where less than 50% of all intervals had a person present (Less People). Overall, otters were statistically more active and less out of sight when more people were present; a weak to moderate correlation was observed between vocalizations and people, a weak correlation was observed between vocalizations and time, and there was no statistically significant difference in vocalizations of the otters in the presence of visitors regardless of staff presence. These results suggest that the measured variables showed no evidence of an overtly negative visitor effect for the otters and may have produced a potentially enriching effect. In addition, measuring multiple variables, including time of day, diurnal patterns, feeding patterns, weather, and providing experimental control could aid in identifying the potential visitor effect for otters and other zoo animals.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020023

Authors: Qianglin Cheng Yirui Ma Yaqi Yuan Yuhan Sun Hong Wu Xubin Fu

Probiotics are widely studied as antibiotic alternatives in commercial aquaculture, yet their effects on fish maintained under long-term aquarium conditions remain poorly understood. This study addressed this gap by evaluating dietary Escherichia coli Nissle 1917 (EcN) supplementation on gut microbiota and inflammatory cytokine expression in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) from a recirculating aquarium system. In this study, hybrid grouper were maintained in triplicate tanks under long-term aquarium environments, and fed a basal diet with 1 × 108 CFU/g EcN (SS group) or a control diet (CS group) for 28 consecutive days. Based on 16S rRNA high-throughput sequencing and qPCR, the intestinal microbiota and expression levels of IL-4, TNF-α, and IL-1β were measured. At the phylum level, the relative abundance of Firmicutes increased from 15.63% (CS) to 66.70% (SS), while Proteobacteria decreased from 76.77% to 30.61%. At the genus level, Exiguobacterium became the dominant taxon in the SS group. Furthermore, EcN supplementation significantly upregulated IL-4 expression and downregulated TNF-α and IL-1β expression. EcN supplementation significantly altered gut microbiota composition, with marked changes in community structure and notable shifts in dominant taxa. Thus, this study provides one of the investigations into EcN-mediated restructuring of intestinal bacterial communities and modulation of host immune transcriptional responses in hybrid grouper maintained under controlled aquarium settings. These findings offer a foundation for designing microbiome-targeted interventions in captive marine fish systems.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020022

Authors: Cameron T. Whitley Kaitlin Barrailler Mary Jackson Theodore Bamberger Marta Burnet

Empathy toward animals has received increasing attention because of its relationship to prosocial attitudes, conservation engagement, and environmental concern. Despite growing interest, the way empathy toward animals is measured varies widely across disciplines and applied contexts, making it difficult to compare findings or assess the strength of existing instruments. This paper examines the measurement landscape of empathy toward animals by identifying and describing tools used in both academic research and conservation practice. A search of Web of Science yielded 2155 unique records, resulting in a final sample of 65 peer-reviewed studies with empathy assessment instruments published between 2000 and 2025. These were supplemented by 42 instruments shared by members of the Advancing Conservation through Empathy for Wildlife (ACE for Wildlife®) Network, one of the largest known networks of professionals focused on enhancing and evaluating empathy toward animals. Across these sources, we observe substantial variation in how empathy is operationalized, including differences in construct emphasis, focal species, intended audiences, and attention to reliability and validity. Academic studies primarily use surveys emphasizing affective empathy toward mammals, whereas practitioner-developed tools are more diverse and often assess cognitive and motivational dimensions across cohort groups. In mapping differences in approaches, we identify persistent gaps and provide suggestions to better align scholarly and applied assessment tools.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020021

Authors: Phoebe Vaughan Ella Slinn Simon Matthews Michelle O’Brien Paul Rose

Moving zoo animals to new enclosures is a normal part of zoo management, but little is known about how animals respond to these changes. Understanding how species adjust after a move can help zoos improve animal management and support smoother transitions between enclosures. This study investigated the behaviour and enclosure use of 121 Chilean flamingos at WWT Slimbridge after they moved from an enclosure that may have housed flamingos for up to 60 years into a newly designed environment. Animal care staff photographed the flock once daily before afternoon feeding and recorded the number of birds foraging, moving, preening, or inactive. The presence of wild birds, visitors, and staff was also recorded to assess possible influences on behaviour. Behaviour in the new enclosure was compared with activity levels previously recorded in the former enclosure. Results showed that flamingos spent more time foraging in the first month post-move and were more often seen in their pool. Activity did not differ between the former and new enclosures. This research provides important information on how flamingos respond to a housing change, showing that birds are more explorative of their new environment before settling into a previously documented routine. Flamingo preening showed an association with wild birds (increase preening) and visitor presence (reduced preening). Such human influences should be further investigated to determine whether flamingos change behaviour due to visitors or whether flamingo activity draws more visitors to the exhibit. Ultimately, our research shows that flamingos settle quickly into a new environment and do not display obvious signs of discomfort when a move is well planned and when enclosure modifications consider species’ natural history. We show the usefulness of a quick-and-easy data collection protocol, implemented by animal care staff, to the continual monitoring and evaluation of zoo husbandry.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020020

Authors: Ellen Bronson Amy Greenebaum Marike Visser Lisa Mangus Kevin Barrett Dawn Boothe

Itraconazole is an antifungal drug used to treat chytridiomycosis, one of the leading causes of global amphibian decline in species such as the critically endangered Panamanian golden frog (Atelopus zeteki). Despite its widespread use for prophylaxis and treatment in both assurance colonies and free-ranging amphibians, there is minimal pharmacologic information to guide dosing. In experiment A, frogs were exposed to 0.01% itraconazole for 10 min and tissue samples were analyzed at various time points from 1 to 84 h. In experiment B, frogs were divided into six groups and exposed to itraconazole in different combinations of concentration (0.01% or 0.001%) and time (5, 10, or 15 min) over 10 days of treatment. Tissue concentrations were quantified via high-performance liquid chromatography. In experiment A, following a one-time dose, itraconazole concentrations remained high until the end of the experiment at 84 h. In experiment B, at 0.01% itraconazole daily for 10 days, skin and liver concentrations were high and increased substantially over the 10-day treatment course. Frogs exposed at the lower concentration (0.001%) had tissue concentrations that appeared to remain steady. At the reported doses over 10 days, there was no histologic evidence of hepatic toxicity, although one frog was found dead in the low-dose bath at 84 h and could not be further analyzed. This experiment is an excellent example of assurance colonies providing evidence-based information for the improved care and welfare of amphibians in order to prepare for future free-ranging populations.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020019

Authors: Michael Kuseni Uwe Peter Hermann Samantha Bouwer

In today’s tourism industry, service quality is essential for differentiation and achieving sustainable competitive advantage. While much research on service quality exists for the private sector, a notable gap remains regarding service dynamics in non-profit public recreational spaces in emerging economies. This study fills that gap by examining service quality (SQ) at National Botanical Gardens (NBGs) in South Africa, focusing on visitor perceptions of SQ importance and performance. Using an adapted Importance–Performance Analysis (IPA) framework, the research explores how visitor expectations compare with actual experiences. A quantitative approach was adopted, with structured questionnaires given to a convenience sample of visitors at key gardens like Pretoria and Walter Sisulu NBGs. Data analysis centred on correlation coefficients to assess the relationship between perceived importance and actual performance. The findings reveal a generally strong, statistically significant positive correlation; however, some attributes show a ‘weak correlation,’ indicating critical service gaps often overlooked by traditional management models. These insights demonstrate that, in natural attraction settings, visitor-perceived importance primarily drives satisfaction with performance. In addition to offering the South African National Biodiversity Institute (SANBI) a strategic plan to boost visitor retention, this study advances the academic discussion by challenging existing SQ models within non-profit recreational sectors. It lays a vital empirical groundwork for future research on public-sector service excellence in the Global South.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020018

Authors: Bojana Z. Jadranin Marija V. Vesović Djordje P. Božović Nikolina A. Matić Michal Goga Milorad M. Vujičić Beáta Papp Aneta D. Sabovljević Marko S. Sabovljević

The rare and threatened pleurocarpous semi-aquatic moss Drepanocladus sendtneri (Amblystegiaceae) was the focus of an integrative conservation approach aimed at improving knowledge of its biological and ecological characteristics and enhancing its survival prospects. The results provide insights into both the axenic and xenic propagation of this species, as well as its biomass production under ex situ conditions. The KNOP medium proved to be the most suitable for propagation, particularly when demeristemized shoot tips were cultured in an upright orientation. Exogenous application of IBA increased the production of new shoots and reduced the time required to obtain substantial biomass under axenic conditions. Following successful acclimatisation to controlled xenic laboratory conditions, the moss was able to fully develop and spread in experimental basins maintained under outdoor botanical garden conditions, with humidity carefully regulated during dry periods. Within one year, a small number of initial plantlets expanded to cover approximately 4 dm2, spreading efficiently over rainwater-soaked filter paper covered with an inert plastic mesh. These results provide practical guidance for the production and ex situ maintenance of D. sendtneri, thereby supporting the development and improvement of conservation action plans for this rare and threatened moss species.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7020017

Authors: Djalma da Nobrega Ferreira Sérgio L. G. Nogueira-Filho Guillermina Hernández-Cruz Stella G. C. Lima Mike Mendl Selene S. C. Nogueira

Understanding how environmental enrichment influences behavioural time allocation is particularly important for threatened primate species maintained under human care. Accordingly, we investigated whether environmental enrichment (EE) influences behavioural time allocation in yellow-breasted capuchin monkeys (Sapajus xanthosternos), aiming to inform evidence-based husbandry practices in zoological settings. Employing the standard ethological approach of behavioural coding, we observed 20 capuchins housed in three groups comprising adult and juvenile males and females. We recorded behavioural categories including: aggressive, exploratory, affiliative/play, general activity, alert, inactivity, and abnormal behaviour. To evaluate individual engagement with EE, we applied the ABA paradigm, wherein phases A1 and A2 (controls) represented standard zoo conditions, while phase B corresponded to the implementation of an EE programme. Each phase spanned 10 days, and behavioural data were collected via focal animal sampling (2 × 10 min focal sessions per animal per day), resulting in a total of 1200 focal sessions. Behavioural time allocation was analysed using a multivariate generalized linear mixed modelling approach that accounted for the interdependence among behavioural categories. Based on previous studies, we predicted that environmental enrichment may promote higher levels of play and exploration and lower aggression and inactivity. However, despite by-eye suggestions of increases in play and decreases in activity during enrichment, when behavioural categories were analysed simultaneously within the multivariate framework, overall behaviour time budgets and behavioural diversity were found not to change significantly across experimental phases. There were also no sex or age effects on behaviour. This indicates that for S. xanthosternos, the enrichment protocol used here did not provide sufficient novelty or complexity to alter established activity patterns. Integrated analytical approaches are needed to further evaluate the effectiveness of enrichment strategies to ensure they are tailored to specific cognitive and social needs of complex species; future studies could explore how social dynamics, enclosure design, and environmental complexity interact to shape behavioural responses to enrichment.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010016

Authors: Isabella C. Fahrenholz Shannon Irmscher John Andrews Tara M. Harrison

Lesser hedgehog tenrecs (Echinops telfairi) are small, nocturnal insectivores from Madagascar that exhibit hibernation and brief torpor bouts. While multiple studies have investigated torpor physiology, no studies have focused on torpor in tenrecs maintained under human care or their recommended husbandry. We surveyed 96 institutions (71.9% response rate) housing 172 tenrecs to assess husbandry, torpor, handling, and associated weight changes. Most institutions reported that torpor occurred annually, typically between October and April, and lasted approximately 5–6 months. Weight distributions differed significantly pre- versus post-torpor, with females and males losing 34.4 g and 20.9 g on average, respectively. Females were heavier than males before and after torpor, the first report of a sex-based weight difference in this species. Most institutions used tenrecs as ambassador animals, and approximately half continued educational programming during torpor. Tenrecs handled more frequently during torpor tended to gain more weight, likely due to increased energy expenditure and compensatory feeding. Tenrecs entered torpor regardless of daylight, temperature, or humidity, with no significant husbandry differences between torpor- and non-torpor-reporting institutions. Torpor is a critical physiological adaptation supporting energy conservation and species survival. Its expression should be supported through appropriate environmental conditions, diet, minimal disturbance, and monitoring.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010015

Authors: Russell L. Barrett

Kings Park and Botanic Garden is a globally recognised botanic garden and research institution in the biodiversity hotspot of southwest Western Australia. A history of the contribution of staff and researchers at Kings Park and Botanic Garden to the knowledge and promotion of the flora of Western Australia is provided. Through an analysis of the published literature, reports, and correspondence with former staff, a strong history of collaborative, world-class research is outlined. This paper focuses on the contributions of staff and students who have discovered and promoted Western Australian flora from the planning and establishment of the Botanic Garden in 1961 up to 2015. Early research was conducted by a small number of individuals with structured research programs emerging much later. Significant contributions have been made in the fields of plant conservation, plant ecology, seed biology, ecosystem restoration, plant systematics and mycorrhizal associations. Key partnerships with universities, other government organisations and industry have been fundamental to the focus and success of research programs.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010014

Authors: Juan Francisco Mota Francisco López-de-Haro Fabián Martínez-Hernández Esteban Salmerón-Sánchez Antonio Jesús Mendoza-Fernández Francisco Javier Pérez-García Federico Carlos Escudero-Clares Paula Mota-Merlo María Jesús Fernández-Cobo Lucía Miranda-Hernández María del Mar González-Beltrán Kevin Hernández-Martínez Encarna Merlo

Modern botanical gardens are essential for conservation, research, education, and recreation. However, recreating habitats with extreme edaphic conditions, such as the Iberian gypsum steppes (priority habitat 1520), poses a significant challenge due to the severe physicochemical constraints of gypsisols. This work aimed to present and evaluate a biomimetic protocol for establishing two gypsum botanical gardens in the southeast Iberian Peninsula, one on a university campus and one at a mining concession, to fulfil all four prototypical functions. The design was biomimetic, replicating the floristic (Gypsophiletalia scrublands) and edaphic characteristics of natural gypsum areas. Crucially, gypsum-milling waste (fines) from the mining operation was repurposed as the main substrate to create the artificial gypsisols. Physicochemical analyses confirmed this strategy effectively replicated the key chemical properties of natural gypsisols, including high CaSO4 concentration, pH, and electrical conductivity, although the artificial soils displayed the low carbon and nitrogen content typical of disturbed gypsum soils. The gardens successfully fulfilled their conservation role by maintaining populations of endemic and threatened gypsophilous species, which flowered and set fruit. The industrial garden validated a research function by serving as a platform for the successful translocation of threatened Narcissus tortifolius bulbs. This project validates a replicable, biomimetic technical protocol that transforms a mining residue into a functional substrate for conservation. The dual model (academic/industrial) maximizes the botanical garden’s functions, offering an effective and highly visible strategy for conserving gypsum biodiversity and countering the social undervaluation of these extreme ecosystems.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010013

Authors: Eleni Kriemadi Venetia D. Nikita Nikos Krigas Pepy Bareka

This study presents an innovative and interdisciplinary didactic approach for teaching biodiversity to high school students, aiming to enhance basic learning skills and promoting deeper understanding of biological concepts. The designed educational proposal aims to support policy-driven collaboration between schools and botanical gardens within the framework of coordinated and long-term educational policies. The intervention was designed to cultivate both cognitive and metacognitive skills within three (3) didactic hours, offering a holistic learning experience through the study of Tulipa members used as an alluring model. A total of 168 Greek first- and second-grade high school students (on average 16 years old) participated in the study. Biodiversity was examined in a multidisciplinary fashion, focusing on species’ diversity (phenotypic), genetic-karyological diversity, and habitat diversity. The core components of the approach included: (a) the completion of a corresponding worksheet module, which effectively engaged students in the learning process using the 5E didactic model, and (b) a karyotype lab experiment using living Tulipa specimens. The learning process was evaluated through two questionnaires assessing the acquisition of scientific knowledge and behavioral outcomes. The results showed a positive impact on students’ understanding regarding the genetic material and biodiversity, with the karyotype experiment playing a key role in achieving high performance in both cognitive and affective learning objectives. Knowledge scores were higher in the second-grade students (53–73%) than in the first-grade students (44–69%) of high school, especially regarding concepts such as karyotype applications and biodiversity-ecosystem balance. The karyotype experiment positively correlated with both the evaluation of the intervention and the shifts in biodiversity beliefs (Pearson’s r = 0.649, 0.515; p < 0.05, respectively). The modeled inquiry-based approach with living tulips and karyotype experiments can help schools and botanic gardens counteract plant blindness by enhancing cognitive and affective learning outcomes within a limited instructional timeframe.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010012

Authors: Flavia Bartoli Maria Antonietta Pontrandolfi Zohreh Hosseini Giulia Caneva

Historic gardens are dynamic archives of biodiversity and cultural heritage, yet many have been lost or transformed, erasing their original floristic identity. This study reconstructs the experimental agricultural garden of Villa Santa Maria in Potenza (Southern Italy). The garden was founded in 1823 by the Società Economica di Basilicata as a provincial center for agronomic innovation. Through a multidisciplinary approach—archival research, iconographic analysis, and taxonomic revision—we analyzed the Catalogus Plantarum Horti Agrarii Lucani (1846), which listed 670 cultivated taxa, including 196 varieties. Updated nomenclature confirmed the presence of 450 plant taxa. The garden featured a clear functional zoning system integrating ornamental parterres, orchards, nurseries, artificial meadows, and experimental plots for industrial crops and forage species. Chorological analysis reveals a dominant Mediterranean component, along with significant Asiatic and American elements, and smaller Australian and African contributions, reflecting 19th-century plant exchange networks. The conservation assessment shows that many historically cultivated taxa are now nationally protected, and several endemics retain high ecological value. These results highlight the role of historic gardens as living laboratories for biodiversity conservation and cultural continuity. Reintroducing documented species and heritage cultivars within a conservation framework can strengthen urban resilience and identity. This case exemplifies how botanical memory can guide landscape planning by linking historical authenticity with contemporary ecological and educational objectives.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010011

Authors: Paw O. F. Christensen Mads H. Clausen Thea L. Faddersbøll Frej Gammelgård Silje M. Lund Alexander P. M. Nielsen Jonas Nielsen Nynne H. Olsen Tobias K. Olsen Sussie Pagh Cino Pertoldi

This study evaluates activity patterns and determines optimal observation periods for assessing the welfare of lowland tapirs (Tapirus terrestris L.) housed in the following two Danish zoological institutions: Aalborg Zoo and Randers Regnskov. The objectives were to identify the most efficient time window for welfare assessments, determine whether machine learning (ML) could support behavioral evaluations by providing automated estimates of activity, and examine whether automated pose-based tracking could serve as a proxy for manual ethogram observations. Behavioral data were collected using standardized ethograms from wildlife camera footage recorded over 72 h. Lowland tapirs were generally more active during daytime, with individuals at Aalborg Zoo showing peak activity between 07:00 and 14:00, while those at Randers Regnskov were most active between 12:00 and 18:00. Activity patterns differed between institutions, with Aalborg individuals displaying concentrated activity peaks and Randers individuals showing more evenly distributed activity. A preliminary ML analysis using the pose-estimation tool SLEAP demonstrated that movement-based activity estimates closely matched manually coded data, suggesting that automated tracking may offer an efficient and non-invasive tool for welfare monitoring. The findings highlight the potential for integrating automated analysis into routine welfare assessments of zoo-housed animals.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010010

Authors: Amaya Thomas John Fieberg Erik Runquist Cale Nordmeyer Seth Stapleton

The Poweshiek skipperling Oarisma poweshiek (Parker, 1870) (Lepidoptera: Hesperiidae) is an endangered prairie obligate butterfly native to the north central United States and southern Canada. Conservation efforts for this species rely on ex situ approaches for population augmentation and reintroductions. As such, improving our understanding of the behaviors of Poweshiek skipperlings and maximizing their reproductive output in an ex situ setting are critical for the success of associated conservation initiatives. In this study, we examined the frequency of breeding behaviors exhibited by Poweshiek skipperlings in relation to various environmental factors: sunlight intensity (measured in lux), ambient temperature, and age. Sunlight intensity was a significant predictor of breeding behavior frequency, but we did not detect an effect of ambient temperature on breeding behavior. We also documented a generally negative relationship between age and breeding behavior frequency for both sexes. The results of our study underscore the importance of natural sunlight in encouraging breeding behaviors in an ex situ conservation environment. Ex situ observations also can help identify environmental conditions that promote high levels of Poweshiek skipperling activity, which could be used to optimize the timing of in situ population surveys.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010009

Authors: Gabriela García-Vázquez Gloria Carrión Antonia Gutiérrez-Mora Martín Mata-Rosas

Vanilla planifolia is an endangered orchid of significant commercial relevance, primarily valued for the natural vanillin derived from its cured fruits. However, its global production faces critical threats due to its limited genetic variability and high susceptibility to phytopathogens, particularly vanilla wilt caused by Fusarium oxysporum f. sp. vanillae. This review synthesizes the recent advances in plant biotechnology, evaluating the efficacy of in vitro culture systems, plant growth regulators, and the implementation of semi-automated temporary immersion systems, as compared to traditional semisolid methods. Emphasis is placed on the pivotal role of physical factors, such as LED lighting, and the symbiotic associations with orchid mycorrhizal fungi to enhance plant growth and vigor. By synthesizing advanced in vitro regeneration protocols, this study establishes a strategic guide for the mass production of high-quality disease-free plantlets. Finally, the impact of these biotechnological tools on ex situ conservation at institutions such as the Clavijero Botanical Garden is discussed, aiming to support the sustainability of the vanilla industry and preserve Mexico’s biological heritage.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010008

Authors: Diego Rivera Adrián Grau Sánchez Guillermo Martínez-López Natacha Vigil José Javier Sigüenza Dennis Johnson Diego José Rivera-Obón Concepción Obón

The invasive red palm weevil (Rhynchophorus ferrugineus, RPW) poses a severe threat to heritage palm collections across the Mediterranean Region, necessitating robust protection strategies. This study addresses the urgent challenge of safeguarding the Spanish Phoenix Collection at Miguel Hernandez University (EPSO) and the Orihuela Palmetum by analyzing the efficacy of applied Integrated Pest Management (IPM) strategies over a ten-year period (2014–2024). Monitoring and treatment protocols included targeted chemical, biological, and cultural controls, and were benchmarked against infestation progression data, climatic trends, and comparative case studies from Murcia, Elche, Nice, and Palermo. Results indicate that a proactive, multi-component IPM strategy, especially when coupled with probabilistic risk models, can significantly improve survival and recovery indicators in Phoenix taxa, although species-specific susceptibility to RPW was a major differentiating factor. Comparative analysis reveals common regional patterns in R. ferrugineus management but underscores the critical need for collection-specific, resource-sensitive protocols for high-value heritage plants, demonstrating that evidence-based best practices and coordinated monitoring are essential for effective heritage palm conservation amid continuous pest pressure.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010007

Authors: Marcelo Bertellotti Verónica D’Amico

The impact of tourism on Magellanic penguins (Spheniscus magellanicus) in Patagonia is a complex issue that requires a balanced approach between conservation and sustainable tourism development. While tourism in the region can bring significant economic benefits, it can also have a negative impact on the penguins by disrupting nesting behavior and chick rearing, and even increasing the risk of disease and predation. We examined a comparative analysis of scientific papers on the impact of tourism on Magellanic penguins in two breeding colonies in Argentinean Patagonia, which have been visited for 10 to 50 years and whose visitor numbers range from 10,000 to 120,000 per year. We analyzed different physiological parameters (i.e., immunological, hematological, biochemical, and stress parameters) and behavioral respond (alternate head turns) in adult birds and chicks in these colonies. Although the results suggest that Magellanic penguins have adapted well to the presence of tourists in their breeding colonies, we documented changes in certain physiological parameters that indicate chronic stress due to high exposure to tourism. It is important to promote sustainable tourism in Patagonia that not only minimizes these negative impacts but also improves the protection of the penguins and their habitat. This includes the creation of new nature reserves, environmental education, and the regulation of tourism activities. Implementing responsible tourism practices can ensure economic benefits while protecting the well-being and health of penguin populations. The combination of increased tourist awareness and concrete conservation measures can protect not only the Magellanic penguins but also the natural wealth of the entire Patagonia region.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010006

Authors: Tai-An Tian Yu Tian Wei Yang Hai-Bo Li Guy Smagghe Jian-Feng Liu Ni Yang Xiao-Long Huang

The Guizhou snub-nosed monkey (Rhinopithecus brelichi), a critically endangered primate endemic to China’s Fanjingshan National Nature Reserve, faces severe population decline, with fewer than 850 individuals remaining in the wild. As a high-altitude species adapted to long, snowy winters, its survival depends on behavioral thermoregulation and energy conservation. However, how these behaviors are expressed in captivity remains unclear. To examine behavioral responses to cold conditions, we analyzed the daily activity rhythms and spatial preferences of R. brelichi under winter conditions. Continuous focal observations and instantaneous scan sampling (every 60 s, 07:00–20:00) were conducted across three consecutive snowy days. The monkeys spent most of their time in sleep, with additional time devoted to awake thermoregulatory behaviors. Spatial use was uneven, with outdoor platform most utilized and indoor ground areas least used. Activity showed distinct daily rhythms, with locomotion peaking in the early morning and evening, and foraging concentrated in the late afternoon. Spatial behavior also displayed cyclical patterns, including consistent outdoor platform use and bimodal reliance on indoor foraging and ground areas. These findings provide the first detailed behavioral and spatial profile of R. brelichi in winter captivity, revealing short-term behavioral adjustments to cold conditions and highlighting constraints imposed by enclosure design. The results offer baseline data for improving welfare and enclosure management for this and other cold-adapted primates.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010005

Authors: Julian E. Garcia-Rejon Carlos M. Baak-Baak Angel G. Canul-Navarrete Rosa C. Cetina-Trejo Julio C. Tzuc-Dzul Wilbert A. Chi-Chim Rodrigo de J. Orozco-Chulin Lourdes G. Talavera-Aguilar

The mosquito fauna was examined at the Mérida Zoological Park, a public site that is highly frequented and is a significant tourist attraction in the Yucatán Peninsula, in southeastern México. Between January 2023 and February 2024, 24 visits were made to the zoo to capture adult and immature stages of mosquitoes. Overall, 12 species belonging to five genera (Aedes, Anopheles, Culex, Ochlerotatus, and Uranotaenia) were documented. A total of 6601 adult mosquitoes of 10 species were captured by aspiration. The adult population of Culex quinquefasciatus Say was the most abundant, accounting for 80.84% (n = 5336) of the collection, while Aedes aegypti Linnaeus represented 11.53% (n = 761). The aviary was the site with the highest capture abundance (n = 2414) and species richness (n = 10). Aedes aegypti was abundant in the zoo restroom (n = 331) and had a higher proportion of blood-feeding females. A total of 1090 larvae and 160 pupae of nine species were collected from 26 mosquito breeding sites, accounting for 25% of positive containers (26/104). Aedes albopictus was frequently found in the larval stage (n = 43) compared to the adult stage (n = 3). The presence of mosquitoes with the potential to transmit pathogens poses a public health risk to zoo visitors and staff.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010004

Authors: Claudia Maria Ferrari Barbosa Diego Rafael Gonzaga Thiago Favares Claudine Massi Mynssen Marcus Alberto Nadruz Coelho Thaís Moreira Hidalgo de Almeida

The Rio de Janeiro Botanical Garden (JBRJ) holds one of the most diverse ex situ palm collections among tropical botanical gardens. This study describes the current composition of the collection, its taxonomic breadth, and its relevance for conservation. In 2025, this inventory documented 1648 individuals from 71 genera and 153 species. About 21% of these specimens are native to Brazil and 76% are exotic, reflecting both historic introductions and the garden’s long tradition in palm acclimatization. The collection includes 17 individuals of 8 native threatened species and 57 individuals of 13 exotic threatened species. Accuracy at the species level improved from 84.2% to 93.3% after identification work, taxonomic revision, correcting misidentifications and standardizing nomenclature. The results reveal gaps in provenance information and a limited representation of native taxa, underscoring the need for targeted additions. Strengthening national partnerships, developing metacollections, and improving provenance records are proposed as key strategies to enhance the conservation value of this living collection. Overall, the JBRJ palm collection serves as an important scientific, educational, and conservation resource and plays a central role in advancing the ex situ preservation of Brazil’s palm diversity.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010003

Authors: Anastasia Chrysomalidou Ioannis Takos Ioannis Spiliotis Panteleimon Xofis

It is widely recognized that outdoor and nature-based educational activities can significantly enhance pupils’ learning, increase environmental awareness and improve pupils’ well-being. At the same time, a growing body of literature supports that the extent to which outdoor learning is implemented depends largely on factors, such as the general institutional context of schools, social and geomorphological aspects and the teacher’s own motivation. The current study employs data collected from 507 primary, middle and high schools in Greece, using a structured questionnaire, and investigates the factors that prevent teachers from engaging in outdoor teaching activities in a green space, as well as those that encourage them to be involved in such educational learning approaches. The results identify institutional barriers, such as the demanding school curriculum, lack of financial resources, limited available time, and insufficient external support, as the main constraints preventing teachers from implementing outdoor activities in nature. On the other hand, it appears that altruistic and intrinsically driven factors, such as personal environmental interest, knowledge of the positive outcomes of environmental education, and a sense of social contribution, are the main factors promoting the adoption of outdoor green education approaches. Availability of green spaces and support by leadership also appear to promote teachers’ engagement in outdoor activities. The findings of the current study highlight the need for educational reforms, to include outdoor, nature-based learning in the school curriculum, provide training and financial support and enhance the confidence of teachers in outdoor, nature-based education. Addressing these barriers could enhance education’s role in fostering sustainable development and reconnecting pupils with nature.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010002

Authors: Lucas Peixinho Campos Nery Tatiane Cezário dos Santos Juliana Martins Ribeiro Natoniel Franklin de Melo

Fruit production in passionfruit species is primarily associated with cross-pollination, but the role of herkogamy, the spatial separation between stigmas and anthers, in reproductive success remains poorly understood. This study evaluated the influence of style deflexion on fruit set in Passiflora cincinnata Mast., a native species of the Brazilian Caatinga, using accessions conserved in a Passionfruit Germplasm Bank (BAG). Flowers were classified into three morphotypes: stigmas positioned below the anthers, stigmas aligned with the anthers, and stigmas positioned above the anthers. Pollen viability was evaluated using subsamples of randomly selected flowers within each floral morphotype. Across all accessions, 41.75% of flowers displayed stigma below the anthers, 26.32% exhibited aligned the stigma with the anthers, and 31.93% had stigma positioned above, with pollen viability consistently high (96.4–96.7%). Flowers in which stigmas were located below the anthers (negative deflexion) showed the highest fruit set under hand pollination (73.3%), whereas flowers with aligned or elevated stigmas produced only a few fruits (4.7%) in heterotypic crosses. These findings demonstrated that herkogamy strongly shapes reproductive outcomes in P. cincinnata. Integrating this knowledge into germplasm management, conservation, and breeding initiatives will be essential for improving passionfruit cultivation and promoting sustainable agricultural practices in semiarid ecosystems.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg7010001

Authors: Xinxin Zhang Jie Wu Chong Wang Fuyong You Peng Liu Yuan Zhang Shaofan Li Yongkang Zhou Yingchao Wang Xiaobing Wu Haitao Nie

Conventional alligator farming, characterized by reliance on chilled fish meat, faces significant challenges, including risks of bacterial contamination and nutritional imbalances. These issues heighten increasing disease susceptibility and threaten industry sustainability, underscoring the critical need for developing nutrient-dense, low-pathogenicity compound feeds. This study conducted a comparative analysis of newborn Siamese crocodiles fed either chilled fish meat or compound feed formulation. Intestinal microbial samples from both cohorts underwent 16S rRNA gene high-throughput sequencing to evaluate differences in microbial composition, diversity, and predicted functionality. The compound feed, specifically formulated for this investigation, possessed the following nutritional composition: crude protein 52.42%; digestible crude protein/digestible energy 16 mg/kcal; crude fat 12.31%; ash 17.42%; crude fiber 0.45%; starch 7.69%; digestible energy 3450 kcal/kg; lysine 3.66%; threonine 1.92%; methionine 1.27%; arginine 3.07%; total essential amino acids 22.97%; calcium 2.51%; total phosphorus 1.8%; available phosphorus 0.98%. Bioinformatics analysis revealed that the compound feed group exhibited numerically higher richness and alpha diversity indices within the intestinal microbiota compared to the chilled fish group. The microbial communities in both groups were dominated by the phyla Proteobacteria, Bacteroidetes, Fusobacteriota, and Firmicutes, collectively representing over 50% of the relative abundance. Functional prediction indicated that the compound feed group possessed the highest relative abundance in metabolic pathways associated with cofactor and vitamin metabolism, carbohydrate metabolism, amino acid metabolism, terpenoid and polyketide metabolism, lipid metabolism, and replication and repair. In contrast, the chilled fish group exhibited significant functional alterations in glycan biosynthesis and metabolism, translation, nucleotide metabolism, transcription, and biosynthesis of other secondary metabolites. Histomorphological analysis demonstrated greater villus height and crypt depth in the compound diet group compared to chilled fish group, although no significant differences were observed in crypt depth or the villus-to-crypt depth ratio. Collectively, these findings indicate that the compound feed enhances intestinal microbial diversity and optimizes its functional structure. Furthermore, while no statistically significant difference in small intestinal villus height was detected, the results suggest a potential positive influence on intestinal development. This investigation provides a scientific foundation for compound feed development, supporting sustainable breeding practices for Siamese crocodiles.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040065

Authors: Sabrina Brando Sara Torres Ortiz Geoff Hosey Heather M. Manitzas Hill

Marine mammals have been successfully maintained under human care; however, the media, public, and professionals within the field frequently voice welfare concerns. This study systematically surveyed peer-reviewed (PR) literature from 1948 to 2024 (n = 1308) and included an opportunistic sample of non-peer-reviewed (NPR) literature from the past 40 years (n = 756) to evaluate research efforts associated with species housed in zoos and aquariums. The current study updates and extends previous efforts to assess research categories. The findings indicate that the volume of research published mirrors the species abundance in human care. Across taxa, PR papers concentrate on science that enhances the understanding of biological functions (Acoustics, Biology, Breeding, Behaviour, Health) but is not necessarily tailored to improve management or optimal care. In contrast, a substantial portion of the NPR literature focuses on daily handling and management, highlighting Environment and Management and Enrichment-related activities. While welfare-related research has increased in both PR and NPR literature, this review underscores the need for additional welfare-related empirical studies to further enhance animal care and wellbeing. We encourage those involved in the practical care of such taxa to empirically evaluate these interventions and disseminate their findings in the PR literature.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040064

Authors: Marco Briguori Pietro Carlino Chiara Carpino Gianni Giglio Francesco Luigi Leonetti Viviana Romano Roberta Castiglioni Emilio Sperone

We quantified how exhibit design and routine management shape behavior and space use in captive harbor seals (Phoca vitulina). Using a species-specific ethogram, scan sampling and focal follows on adult females housed in a modern zoo exhibit, we estimated time budgets, mapped space use across depth-defined zones, and modeled behavior sequences as first-order transition networks. Locomotion dominated activity (swimming/active travel), with resting and enrichment-related behaviors next most frequent; social and play behaviors occurred at low but non-negligible rates. Seals showed clear depth preferences, concentrating active swimming in deeper zones and using liminal/shallow areas for rest. Transition graphs revealed stable, low-entropy loops (e.g., swim → turn/pace → swim) consistent with repetitive locomotor routines, while enrichment and feeding windows temporarily diversified sequences and increased exploration. Overall, integrating time budgets with Markov-style transition analysis and spatial heatmaps provides a compact welfare-oriented dashboard: it identifies where exhibit depth and refuge availability support positive behavioral diversity, flags repetitive cycles as targets for enrichment, and offers actionable metrics to evaluate husbandry changes over time.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040063

Authors: Boris L. Kozlovsky Olga I. Fedorinova Mikhail V. Kuropyatnikov Mikhail M. Sereda Anastasiya A. Dmitrieva Pavel A. Dmitriev

The Botanical Garden of Southern Federal University (SFedU Botanical Garden) is the first botanical garden in the steppe zone of southern Russia, founded in 1927. The priority task of the SFedU Botanical Garden was the introduction of woody plants for greenery and forestry. It has been shown that the introduction of woody plants was the root cause of their invasion in the region. A total of 24 species of invasive trees and shrubs have been identified in the Priazovsky district of the Rostov region. Using species with high seed reproductive capacity and resistance to climatic factors to expand the range of woody plants used for greenery in urban areas poses a real threat of invasion. Thus, 83 species spread spontaneously from the SFedU Botanical Garden collections across its territory, 50 of which are not currently found in the regional culture. An important step in the management of invasive woody plants is for municipalities to adopt basic assortment lists for greening purposes. The SFedU Botanical Garden’s collection policy for woody plants should focus on reducing the number of species in living plant collections by removing species that self-seed and currently have no scientific, educational, or practical use. These species can be stored in a seed bank for future use. The introduction policy of the SFedU Botanical Garden should be aimed at mobilizing and introducing species that are not only highly resilient and effective in providing ecosystem services, but also possess properties that limit their invasion.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040062

Authors: Tsvetan Tsvetkov Denica Daskalova

Honey bees (Apis mellifera) are the main pollinators of many plant species, particularly agricultural crops. The concern over Colony Collapse Disorder of bee colonies in recent years necessitates the use of new approaches for their conservation in in situ and ex situ conditions. Modern techniques for cryopreservation of drone spermatozoa allow for the preservation of their genetic diversity. Some of the challenges in the field of cryopreservation are the alterations induced by the low temperatures, including morphological disruptions, plasma membrane integrity, formation of reactive oxygen species, DNA fragmentation, loss of motility, mitochondrial activity and viability, early hyperactivation, depletion of proteins from the acrosome region, premature capacitation, reduced sperm–oocyte fusion, and the occurrence of other cellular cryoinjuries. The objective of the current study is to contribute to the ongoing efforts in identifying substances added to semen extenders aimed at inhibiting cryogenic-induced changes. Our study investigates the impact of antioxidant supplements, scilicet vitamins C, vitamin E, and L-carnitine, on attenuating the adverse effects of cryogenic storage on drone spermatozoa. Using a Computer-Assisted Sperm Analysis, we evaluated the effectiveness of various antioxidants added to the extender in maintaining sperm motility parameters following liquid nitrogen storage. The data indicated significant differences in sperm traits among treatments with supplements after post-thawing. These findings emphasize the advantageous contribution of these added antioxidants within semen extenders for drone spermatozoa in preserving sperm quality parameters. The establishment of novel protocols for cryogenic storage of honey bee drone spermatozoa, incorporating low-cytotoxicity additives, is of utmost importance for the conservation of this endangered species.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040061

Authors: Stacey J. Higgs Maren Huck David Sheffield Dean Fido

Zoological establishments have five main roles: education, conservation, recreation, research, and provision of positive welfare to exhibited and housed animals. This review highlights the effect direct human–animal interactions had on the non-human animals’ physical and psychological wellbeing in relation to short- and long-term education and conservation engagement and action. The literature was reviewed from worldwide English sources, which focused on welfare, direct animal interactions, and alternative provisions. With over 700 million visits made to zoological establishments annually, only USD350 million in funds were reported to be reinvested into conservation efforts worldwide, with the true outcome and impact of this effort unknown. Currently, the main focal area is recreation, with 75% of those surveyed (N = 1241) worldwide found to offer direct human–animal interactions. This review takes a holistic approach to human–animal interactions and highlights key opportunities that are missed in the delivery method of these. Worldwide, direct human–animal interactions during the last 30 years have had no to limited regulations. Organisations such as Pan-Africa Association of Zoos and Aquaria and British and Irish Association of Zoos and Aquaria aim to regulate and monitor welfare, breeding, and husbandry within collections; however, joining is entirely voluntary. This has led to only 13 zoological establishments in Africa accredited under PAAZA, compared to over 100 establishments accredited under BIAZA in the United Kingdom. This review highlights that clear training plans need to be implemented before and during human–animal interactions with particular focus on feeding time demonstrations, CGI models, film rooms, and no-contact interactive areas. Further research needs to quantify the significance of the taxonomy used in direct interactions.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040060

Authors: Bertrand Ng Guillaume Douay Trent Charles van Zanten Yirui Heng Ali Anwar Ahmad Chia-Da Hsu

Type 2 diabetes mellitus (T2D) is a well-studied condition characterized by increased insulin resistance, β-cell dysfunction and amyloid deposition in the pancreatic islets. The condition is best understood in humans and veterinary species such as the domestic cat but is poorly described in zoo animals. A 16-year-old male binturong (Arctictis binturong) at Mandai Wildlife Reserve presenting initially for a left forelimb tremor was noted to have hyperglycemia (23.86 mmol/L; RI 2.93–18.94), with concurrent glucosuria, which persisted over 2 weeks. The animal was diagnosed with diabetes mellitus based on serial biochemical and urinalysis results and ultrasonography. No pharmaceutical treatment was instituted. Over 4 years the animal developed other age-related changes and was euthanized based on a declining quality of life. Postmortem investigation including histological examination found diffuse islet amyloidosis of the pancreas, consistent with T2D as seen in other species. To the authors’ knowledge, this is the first published case report of type 2 diabetes mellitus and pancreatic amyloidosis reported in the binturong. The popularity of the species in zoological institutions and long-term implications of the disease warrant further attention. Further research may be directed toward establishing diagnostic values in binturongs, as well as options for medical management.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040059

Authors: Oscar Sandino Guerrero-Eloisa Jordan Golubov María C. Mandujano Beatriz Maruri-Aguilar

Botanical gardens promote the conservation of native species of the sites where they are located, showing the importance of preserving native flora. It is common to find invasive alien species (IAS) established intentionally or accidentally in these sites. In a patch of native vegetation within the Cadereyta de Montes Regional Botanical Garden, the removal of the invasive alien Asphodelus fistulosus was carried out and during a subsequent 108-month period. The repopulation of the species was supervised by removing new individuals at each visit. More than 1000 individual plants of A. fistulosus were removed during the entire monitoring period, and through subsequent visits, no new individuals have been spotted. The monetary cost associated with the removal, monitoring and control of A. fistulosus was calculated over MXN 15,000.00. The removal and post-removal monitoring of species at sites of interest provide valuable information about control time and economic costs to consider in the control of IAS. Additionally, the germination experiments carried out with seeds from different years of collection showed a higher germination percentage that was not affected by the age of the seeds.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040058

Authors: Tatiana Vdovina Olga Lagus Elena Isakova Aidar Sumbembayev

Hippophae rhamnoides L. (sea buckthorn) is a key multipurpose shrub of Eurasia valued for its ecological resilience, nutritional properties, and economic importance. This study examines the regenerative capacity, cold tolerance, productivity, and vegetative propagation efficiency of H. rhamnoides populations originating from East Kazakhstan and maintained at the Altai Botanical Garden (ABG). Between 1981 and 2024, five natural populations (Kendyrlyk, Kaindysu, Tersayryk, Shetlasty, and Karatal Sands) were evaluated under both natural and introduction conditions. A total of 68 clonal forms were propagated and assessed for longevity, yield stability, and morphological traits. The results demonstrated high ecological plasticity and adaptation to the sharply continental climate of East Kazakhstan, withstanding winter temperatures of −38 to −44 °C without damage. Long-lived genotypes (up to 32 years) exhibited consistent productivity, yielding 3.7–14.5 kg per plant (4.6–17.5 t/ha). Large-fruited cultivars such as ‘Yubileinaya Kotukhova’, ‘Shetlastinka’, and ‘Asem’ reached fruit masses up to 95.8 g of 100 berries. Vegetative propagation by green cuttings proved highly effective, with rooting rates up to 90% when treated with HB-101, exceeding control treatments by 14.7%. Stable thickets formed by root suckers persisted for nearly four decades, confirming strong clonal stability and adaptive capacity. These findings underscore the significant potential of H. rhamnoides germplasm from East Kazakhstan for breeding cold-hardy, high-yielding cultivars suited to continental climates. This research highlights the importance of ex situ conservation at ABG and provides a foundation for further genetic, biochemical, and breeding studies aimed at enhancing the productivity and sustainability of this ecologically and economically valuable species.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040057

Authors: Menglin Sun Hongyu Yao Nan Wu Hong Wu Dapeng Zhao

The gut microbiota plays a crucial role in the health and well-being of primates. This study applied 16S rRNA high-throughput sequencing technology, aiming to investigate the differences in gut microbiota composition and function between captive ring-tailed lemurs (Lemur catta) in different zoos across the north–south demarcation line in China. Results revealed significant differences in gut microbiota composition between northern and southern groups. Alpha diversity indices were higher in the southern group (p < 0.05), while beta diversity analysis showed distinct clustering based on geographic location (p < 0.001). Bacteroidetes were more abundant in the northern group (49.22% vs. 28.44%), while Firmicutes predominated in the southern group (59.10% vs. 32.78%). Functional prediction analysis indicated higher levels of membrane transport and lipid metabolism pathways in the southern group, suggesting differences in nutrient absorption and energy metabolism. These findings suggest that geographic location and associated environmental factors significantly influence the gut microbiota of captive ring-tailed lemurs, even under similar dietary and husbandry conditions. Our study provides insights into the impact of geographic location on gut microbiota in captive primates, highlighting the importance of considering regional factors in zoo animal management and informing future strategies for optimizing the care and conservation of captive primates across different geographic regions.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040056

Authors: Navapong Subpayakom Pongrat Dumrongrojwattana Supattra Poeaim

Rivomarginella morrisoni is a freshwater snail endemic to Thailand, yet its behavioral ecology remains poorly understood. This study described the feeding behavior of R. morrisoni, focusing on its foraging activity, behavioral patterns, and food detection mechanisms under laboratory conditions using specimens collected from four river basins in central Thailand. Daily monitoring revealed nocturnal emergence, peaking between 21:00 and 22:00 h, with stable rhythms established 72 h post-feeding. Feeding trials revealed a preference for aged shrimp over fresh or decayed ones. Behavioral observations confirmed that food localization in R. morrisoni was mediated by chemical cues. Light–dark tests indicated a slight tendency toward darkness, but no significant phototactic response was observed. These findings suggest that R. morrisoni is a generalist scavenger with chemosensory-driven foraging and nocturnal activity. Its apparent sensitivity to habitat disturbance underscores the relevance of behavioral studies for informing future conservation and captive breeding efforts.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040055

Authors: Anđelka Štilić Jurica Bosna Adis Puška Miroslav Nedeljković

This paper evaluates botanical gardens in terms of their potential for tourist valorization, aiming to identify the garden with the highest tourist appeal and integration opportunities within the tourist market. Based on a literature review and established attractiveness criteria, a methodological framework using multi-criteria decision-making was developed to compare and rank the botanical gardens. The empirical part of the study focuses on botanical gardens in Split–Dalmatia County, including six gardens evaluated across nine criteria. Eight local tourism experts assessed the importance of these criteria and the gardens’ performance. The fuzzy SiWeC (SImple WEight Calculation) method was used to determine the importance of each criterion. The fuzzy TOPSIS method (Technique for Order Preference by Similarity to Ideal Solution) was used to measure the potential of botanical gardens. The main results obtained with this approach showed that the most important criteria are C4—Visitor content and C3—Biodiversity conservation. The Botanical Garden of Primary School Ostrog has the greatest potential, followed by the Botanical Garden Split. All observed botanical gardens have excellent tourist potential, with minimal differences in ranking among them. These findings demonstrate that botanical gardens play a key role in diversifying the tourist offer, reducing seasonality, and increasing the overall attractiveness of destinations. They also contribute to raising environmental awareness and emphasizing the importance of nature conservation and sustainable development, aligning with the increasing tourist interest in natural and ecologically responsible experiences. This study offers practical insights, as the results can assist garden management, tourism communities, and policymakers in developing and promoting strategies. Additionally, the framework proposed can be applied in other regional and international contexts.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040054

Authors: Alejandro Torres-Montúfar Mayte Stefany Jiménez-Noriega Karla Quiñones-Molina

The Metropolitan Area of the Valley of Mexico (MAVM), home to more than 20 million inhabitants, faces intense urban and industrial pressures that have led to habitat loss, fragmentation, and a decline in native vegetation. Within this context, botanical gardens are key institutions for ex situ conservation, ecological restoration, education, and cultural preservation. This study proposes the establishment of a collection of native and endemic ornamental plants in the Botanical Garden of the Facultad de Estudios Superiores Cuautitlán (FESC-UNAM), the only botanical garden located in an industrial zone of Mexico. To design this proposal, we conducted an extensive review of taxonomic, floristic, ethnobotanical, and horticultural literature, with special reference to the Flora Fanerogámica del Valle de México, and complemented it with exploratory field visits. The resulting collection comprises 35 native species across 26 families, including 17 endemic species, all adapted to xerophilous scrubland. The discussion highlights that beyond aesthetic value, many species have medicinal and cultural significance, strengthening the link between biodiversity conservation and social engagement. We conclude that this proposal enhances the dual role of the FESC Botanical Garden in preserving regional flora and promoting sustainable alternatives for urban landscaping in industrialized environments.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040053

Authors: Kaiwen Zheng Hui Fu

This study investigates the applicability of the Japanese forest education model, exemplified by the Whole Earth Nature School (WENS), to environmental education programs within urban parks and botanical gardens. Focusing on WENS’s approach—characterized by immersive natural experiences, innovative facilitation techniques, and support from interdisciplinary educator teams to foster independent thinking through experiential learning—the research identifies three transferable insights: (1) Reorienting educational philosophy to prioritize nature-based learning for holistic development, particularly within urban green spaces; (2) Developing localized curricula that leverage the unique ecological resources of specific park and garden environments; (3) Establishing dedicated professional development systems for educators focused on site-based pedagogy. The findings demonstrate that adapting the forest education model can significantly enhance environmental education initiatives in urban parks and botanical gardens, which offers actionable strategies for integrating experiential nature learning into their educational planning.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040052

Authors: Arthur Afeitos Silva Cristiano Schetini de Azevedo Cynthia Fernandes Cipreste Cristiane Schilbach Pizzutto Eneida Maria Eskinazi Sant’Anna

In the original publication [...]

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040051

Authors: Julia Jenikejew Mascha Huelsewig Damaris Riedner Mathilde Stomp Alban Lemasson Martine Hausberger Idu Azogu-Sepe Martin Böye Marina Scheumann

In southern white rhinoceroses (Ceratotherium simum simum), pant calls are well-studied contact vocalisations, whereas the function of frequently emitted snorts remains unclear. We conducted playback experiments with 15 rhinoceroses at three European zoos. The first experiment tested responses to conspecific versus heterospecific snorts, comparing pulsed and non-pulsed acoustic structures. The second experiment contrasted conspecific snorts with conspecific pants from males differing in age and faecal testosterone metabolite (fTM) levels. Behavioural responses—including body orientation, approach toward loudspeaker, locomotion, and vocalisations—were analysed. Snorts, regardless of sender species or pulsation, elicited uniformly low-intensity responses, suggesting limited communicative function. In contrast, pants evoked significantly stronger responses depending on sex and group setting. While males showed increased locomotion, females vocalised more, reflecting the species’ social dynamics. Individuals tested alone displayed overall heightened vigilance and vocal activity compared to those tested in pairs, emphasising the role of social context. No evidence was found for discrimination between pant calls differing in fTM levels. Our findings reinforce the communicative relevance of pants in conveying social cues while indicating that snorts may either lack species-specific acoustic markers or not be socially salient. Playback experiments thus appear as valuable tools for assessing acoustic communication in zoo-housed mammals.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040050

Authors: Andressa Sasha Quevedo Alves Oliveira Rafaela Jemely Rodrigues Alexandre Simone Almeida Pena Letícia Lima Correia Thais Santos Souza Samantha Valente Dias Thiago Bernardi Vieira Felipe Bittioli R. Gomes

The species of the Bufonidae family exhibit a great diversity of habitats, diurnal or nocturnal habits, a complex evolutionary history, and a wide distribution, which makes this group suitable for morphological studies. In this work, we aimed to identify the existence of morphological patterns related to the habitat use and diurnal or nocturnal habits of Bufonidae in the Brazilian Amazon. To achieve this, we studied the morphological measurements of 210 specimens from three zoological collections and characterized the type of habitat and diurnality/nocturnality of the species. The morphological patterns and habitat use were investigated through principal component analysis (PCA) and multiple correspondence analysis (MCA), respectively. The evaluation of the relationships between morphological variation, habitat use, and diurnality/nocturnality was performed via redundancy analysis (RDA). Accordingly, Amazonian bufonids were divided into three morphological groups associated with different vegetation types and environments, demonstrating that body size is closely linked to diurnal or nocturnal life habits and habitat. Species with large body sizes are associated to anthropized areas, while intermediate and smaller species are associated with primary forests.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6040049

Authors: Stefany González Andrea Caiozzi Osvaldo Cabeza Hernan Cañon-Jones

Amphibian welfare, particularly pain assessment in anurans, remains understudied despite their ecological and biomedical significance. This study aimed to identify and validate operational pain indicators for adult anurans under professional care. A four-phase approach was used: a systematic literature review, expert validation with risk analysis, field validation in a zoological facility, and development of a preliminary pain index. From 158 publications, 16 potential indicators were identified, encompassing behavioural, clinical, and physiological signs. Expert evaluation by 28 professionals from 12 institutions refined this to seven indicators, achieving over 60% consensus: feeding behaviour changes, abnormal behaviour, impaired locomotion, oedema, reduced movement, retained skin post-moulting, and altered respiration. Field validation in 53 anurans confirmed high observability and ease of measurement, with feeding behaviour changes and oedema scoring highest for practicality (93.5% and 93.0%, respectively). These validated indicators provide a science-based foundation for routine welfare monitoring, enabling timely interventions. Their integration into husbandry protocols can enhance ethical standards, improve conservation outcomes, and increase public confidence in amphibian care, paving the way for a standardised anuran pain index.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030048

Authors: Jessica C. Whitham Lance J. Miller

In recent years, animal welfare scientists working in professionally managed settings have increasingly focused on promoting resilience to enhance the quality of life of individual animals. Resilience—defined as an animal’s capacity to be minimally affected by a disturbance or to rapidly return to the physiological, behavioral, cognitive, health, affective, and production states that pertained before exposure to a disturbance—involves various systems and dynamic processes. There is evidence that resilience can be measured using a suite of species-specific indicators, including both behavioral measures and physiological biomarkers. These indicators should be tracked for individuals of the same species over time and across various conditions, events, and experiences. Large-scale, multi-institutional studies allow welfare scientists to collect cross-sectional data to identify “resilient phenotypes” for the species of interest. Ultimately, the focus should be on improving outcomes for individual animals as they face particular stressors, challenges, and environmental disturbances over their lifetime. Animal care specialists play a crucial role in helping animals build resilience by providing opportunities to engage in cognitive challenges, stimulating environments, and species-appropriate social interactions. This review defines resilience for animal welfare scientists, as well as discusses how to measure and promote resilience in animals residing in zoos and aquariums.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030047

Authors: Paul Rose Tom Rice

Although long neglected, sound is now an increasing topic of interest in zoo and aquarium science. Research has examined the impact of sounds, from various sources, on zoo-housed species, noting that the influence of sound is varied and very context specific. The zoo’s sound environment is influenced by the animals, the built environment, vegetation, climatic, temporal and seasonal factors, equipment use, husbandry practices, and human presence. Different sounds can dominate an enclosure at certain times. This article discusses a workshop involving 12 zoo professionals, held in March 2020, that explored how sound is considered or overlooked in zoo animal management. Although insights are based on a small group, limiting generalisability, the findings highlight areas where further understanding is required and should encourage research extension to other groups of stakeholders. Delegates emphasised that the auditory needs of animals are often underappreciated and that the influence of sound depends on the species involved and how the sound may be perceived. Delegates highlighted the importance of species- and individual-specific approaches, predictability, and how animals have (any) control over sounds experienced in their enclosure. Routine operational sounds, such as closing gates or doors, may inadvertently stress animals, suggesting the need to consider sound in enclosure design and husbandry schedules. Outputs also stated that sound, when carefully managed, can act as enrichment through (for example) structured auditory cues or naturalistic sounds if ecologically relevant. Overall, our findings support integrating sound measurement into broader welfare assessment frameworks and enclosure planning, and they identify practical applications including sound mitigation, enrichment strategies, and staff training. Future research should include a wider range of species (especially understudied taxa), consider the experiences of a wider stakeholder demographic, and evaluate how sound is management in complex or high-traffic areas of the zoo.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030046

Authors: Alejandro Galindo-Durán

This study investigates the impact of a gamified educational experience on the training of future teachers, focusing on a visit to the Natural History Pavilion at the University of Almería, compared to a traditional instructional approach. A quasi-experimental pre-test–post-test design was employed with both undergraduate and master’s degree teacher education students, combining quantitative analysis with complementary data. The findings indicate that participants in the gamified groups demonstrated significant gains in knowledge acquisition following the visit, as well as higher levels of appreciation for the activity itself. This study concludes that gamification cognitively enriches the museum learning experience and stands out as an innovative and pedagogically relevant strategy in teacher education.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030045

Authors: Katerina Zareva-Simeonova Venislava Spasova Daniela Simeonovska-Nikolova Krastio Dimitrov Vladimir Todorov Kalina Valchinkova

Assessing the well-being of captive brown bears is essential for responsible species management and meeting European and national animal care standards outside their natural habitats. The present study aims to assess the welfare of captive brown bears in Bulgaria, considering environmental parameters, husbandry procedures, and the psychological and physical condition of the animals. The study was conducted on 28 brown bears in 11 zoos in Bulgaria and 19 bears in Bear Sanctuary Belitsa. A specific questionnaire and interviews were conducted with zoo managers and keepers. The welfare of the bears was evaluated based on their living conditions, husbandry, and physical and psychological well-being. The Nature and Animal Protection Center in Dobrich received the highest rating for the welfare of two brown bears among the zoos in the study. The study identified annual veterinary check-ups and enrichment programmes as areas needing improvement.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030044

Authors: Neil Chace Susan W. Margulis

Many zoos display animals in mixed-species exhibits where multiple different species share the same space and potentially interact. This study analyzes a mixed-species exhibit with three New World monkey species (white-faced saki, black-capped squirrel monkey, and common squirrel monkey) at the Buffalo Zoo to determine the interactions among species and how different species use the exhibit space differently. Data were collected over twelve months using scan sampling. The sakis were more likely to be in proximity (less than 1.5 m apart) with others than were the squirrel monkey species. The sakis spent 26% of the time in contact with another animal, while both squirrel monkey species spent less than 1% of the time in contact with another animal. The squirrel monkeys used significantly more of the exhibit space than the sakis. A small number of observations occurred when only the sakis were on exhibit, and while speculative at best, anecdotally the sakis used much more of the exhibit when the squirrel monkeys were not on exhibit. There are many compelling reasons for zoos to design mixed-species exhibits; however, consideration needs to be given to how mixed-species exhibits impact animal behavior.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030043

Authors: Luciana Diniz Rola Fabiana Fernandes Bressan Eluzai Dinai Pinto Sandoval Jacinthe Therrien Lawrence Charles Smith José Maurício Barbanti Duarte

The capability to generate induced pluripotent stem cells (iPSCs) from adult somatic cells, enabling them to differentiate into any cell type, has been demonstrated in several studies. In humans and mice, iPSCs have been shown to differentiate into primordial germ cells (PGCs), spermatozoa, and oocytes. However, research on iPSCs in deer is novel. Despite the necessity for establishing germplasm banks from endangered cervid species, the collection and cryopreservation of gametes and embryos have proven complex for this group. Therefore, the focus of this study was to establish protocols for deriving stable iPSC lines from Blastocerus dichotomus (Marsh deer) using primary cells derived from antler, adipose tissue, or skin, with the ultimate goal of producing viable gametes in the future. To achieve this, two main reprogramming approaches were tested: (1) transfection using PiggyBac transposons (plasmid PB-TET-MKOS) delivered via electroporation and (2) lentiviral transduction using the STEMCCA system with either human (hOSKM) or murine (mOSKM) reprogramming factors. Both systems utilized murine embryonic fibroblasts (MEFs) as feeder cells. The PiggyBac system was further supplemented with a culture medium containing small molecules to aid reprogramming, including a GSK inhibitor, MEK inhibitor, ALK/TGF inhibitor, and thiazovivin. Initial colony formation was observed; however, these colonies failed to expand post-selection. Despite these challenges, important insights were gained that will inform and guide future studies toward the successful generation of iPSCs in deer.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030042

Authors: Juan Casas-Paul José Luis Bravo-Ramos María Guadalupe Sánchez-Otero Sokani Sánchez-Montes Sashenka Bonilla-Rojas Luis Arturo Ortíz-Carbajal Gerardo Gabriel Ballados-González Jannete Gamboa-Prieto Alejandra Chong-Guzmán Angelica Olivares Muñoz

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae pose a significant public health risk. As zoos grow in popularity, exotic animals come into closer contact with humans, making them potential reservoirs of ESBLs. However, data on ESBL presence in Mexican zoos remains limited. For this reason, this study aimed to isolate and assess the antimicrobial susceptibility of Enterobacteriaceae that colonize avian species in a petting zoo and to identify any ESBL-producing isolates. Cloacal swabs were collected from 34 healthy birds at Miguel Angel de Quevedo Zoo, Veracruz, Mexico. Samples were analyzed microbiologically and molecularly to detect ESBL-encoding genes. A total of seventeen E. coli and one K. pneumoniae strains were isolated from cloacal swabs of bird species, and multidrug resistance (MDR) was found. The most frequently detected genes were blaCTX-M-1 (16/18) and blaTEM-1 (12/18). The detection of multidrug-resistant (MDR) strains carrying blaCTX-M-1, blaTEM-1, and blaSHV genes highlights the potential role of birds as reservoirs and disseminators of antimicrobial resistance (AMR) in urban environments. To the best of our knowledge, this is the first study conducted in Mexico. In conclusion, MDR ESBL-producing bacteria were found in the fecal microbiota of bird species at a petting zoo in Mexico. The limitations of this study emphasize the need for a One Health approach to analyze the genome-wide isolates and epidemiology of antimicrobial-resistant bacteria in captive zoo animals in Mexico. This would support targeted surveillance efforts and help reduce the emergence and spread of resistant bacteria among zoo animals and visitors.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030041

Authors: Andrea D. Larrea Solórzano Iván P. Álvarez Lizano Pablo R. Morales Fiallos Carolina E. Maldonado Cherrez Carlos S. Suárez Naranjo

This study explores the use of chlorography as a natural photographic developing technique that utilizes the decomposition of chlorophyll and other plant pigments through the action of sunlight. The developed images corresponded to previous research on changes in the iconography of the indigenous Salasaka people. In this context, this experimental project on natural photography is oriented toward the conservation of the ancestral knowledge of this community and the understanding of the native flora of Ecuador. We investigated the application of the contact image transfer technique with positive transparencies on leaves and flowers of 30 different species that grow in the Ecuadorian highlands, including leaves of vascular plants, as well as rose petals. The results showed that the clarity and contrast of chlorography depended on the plant species and exposure time. It was observed that fruit-bearing species produced more visible images than the leaves of other plants and rose petals, with species from the Passifloraceae family proving particularly effective. We interpreted these findings within the framework of plant photophysical mechanisms, proposing an inverse relationship between development efficiency and species’ non-photochemical quenching (NPQ) capacity. Furthermore, we interpreted the findings in relation to the photobleaching of pigments and compared chlorography with other natural photographic processes such as anthotypes. Key factors influencing the process were identified, such as the type of leaf, the intensity and duration of light, and the hydration of the plant material. It is concluded that chlorography is a viable, non-toxic, and environmentally friendly photographic alternative with potential applications in art, education, and research, although it presents challenges in terms of image permanence and reproducibility.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030040

Authors: Todd L. Schmitt Thomas H. Reidarson James F. McBain Hendrik H. Nollens Amber P. Sanchez David M. Ward

An adult female bottlenose dolphin (Tursiops truncatus) housed at a public oceanarium presented with acute anorexia and lethargy. A blood analysis demonstrated mild leukocytosis, marked azotemia, hyperkalemia, and hyperphosphatemia suggestive of acute kidney injury or renal insufficiency. Ultrasound examination of the dolphin revealed ascites, pleural effusion, bilateral nephrolithiasis, mild hydronephrosis, and bilateral hydroureter consistent with bilateral post-renal obstruction. Initial treatment consisted of antibiotics, oral fluids, and anti-inflammatory treatment. Further imaging diagnosed bilateral obstructing ureteroliths at both ureteral orifice junctions of the urinary bladder. The dolphin’s azotemia and hyperkalemia were nonresponsive to traditional medical management; therefore, peritoneal dialysis was performed for emergent clinical stabilization. Peritoneal dialysis was conducted over 3 days and facilitated the patient to undergo laser lithotripsy of the offending ureteral obstruction. The dolphin made a full recovery following months of intensive medical treatment for complications from peritoneal dialysis and secondary peritonitis. This is the first documented case of successful, though complicated, peritoneal dialysis in a cetacean.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030039

Authors: Danielle Walker Paige Bwye Sarah Richdon

Zoological environments aim to promote natural behaviours and optimal welfare conditions. Over the past decade, research on the use of artificial ultraviolet-B (UV-B) exposure has improved vitamin D3 levels and reduced incidences of metabolic bone disease in diurnal primates; however, this has not been investigated in nocturnals. Aye-ayes (Daubentonia madagascariensis), nocturnal lemurs often housed indoors in zoos with little to no exposure to natural sunlight, have been reported to have low vitamin D3 levels. This study aims to investigate the impacts of artificial UV-B as a supplemental healthcare strategy for aye-ayes, examining its influences on vitamin D3 levels and positional sleeping behaviour. The 25-hydroxy-vitamin D3 (25OHD3) blood levels were tested before and after exposure to different levels of artificial UV-B and heat sources. Statistical analysis showed no correlation between UV-B and 25OHD3 at group parameter levels. However, one individual showed a positive correlation. Sleeping position duration analysis showed a potential basking behaviour with the use of increased ear exposure and other thermoregulatory responses. Despite representing 8.06% of the European captive aye-aye population, these findings highlight the need for further research on vitamin D3 parameters and responses to UV-B to optimise captive conditions and support the species’ long-term health.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030038

Authors: Courtney Archer Joselyn Hoyt Emma Loy Emma Marthaler Abigail Richardson Katie Hall Madison Bacon Rielle Perttu

Environmental enrichment is essential for promoting species-specific behaviors and enhancing the welfare of zoo-housed animals. This study examined the behavioral responses of two juvenile male binturongs (Arctictis binturong) at the Minnesota Zoo to three enrichment stimuli: lavender oil (olfactory), thawed fish (olfactory and dietary), and hard-boiled egg (olfactory and dietary). Their behaviors were recorded using scan sampling before and after enrichment exposure, focusing on locomotion, foraging, resting, and visitor visibility. Food-based enrichments, particularly the hard-boiled egg, significantly increased foraging behavior, while lavender oil and thawed fish produced minimal behavioral changes. Locomotion and visibility remained stable across the conditions, although a slight increase in resting was observed with lavender oil. No evidence of scent-marking disruption was noted, and individual differences appeared to influence inactivity levels. These findings highlight the potential of biologically relevant, food-based enrichment to stimulate natural behaviors in binturongs and emphasize the importance of species-specific enrichment strategies. Future research should explore a broader range of olfactory cues, assess long-term behavioral responses, and incorporate physiological measures to further evaluate enrichment impacts on binturong welfare.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030037

Authors: Jovana Ostojić Tijana Narandžić Milica Grubač Lazar Pavlović Mirjana Ljubojević

Botanical gardens serve as vital centers for ex situ conservation, maintaining diverse plant species under controlled conditions. Terrestrial orchids, despite their wide diversity and distribution, often occur in small and declining populations, making their conservation increasingly urgent. This study aimed to examine the potential for establishing the first specialized orchidarium in Serbia, focusing on the native orchid species of the Fruška Gora region. A SWOT analysis, combined with site assessment data, was employed to identify key strengths, weaknesses, opportunities, and threats, informing the development of a functional zoning plan. The results indicate that such an orchidarium would offer a threefold benefit: strengthening ex situ conservation, advancing scientific research and environmental education, and promoting sustainable tourism. The proposed design consists of eight distinct zones, three of which reflect natural habitats of selected orchid species. The planned integration of a seed gene bank in the central zone, along with living plant collections and a nearby in vitro culture laboratory, establishes a comprehensive framework for the sustainable management of orchid genetic resources in the region, forming a foundation for future research and preservation.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030036

Authors: Joy Kubarek Amanda Lindell Shelly Grow Jackie Ogden

This contributed paper presents results from efforts by the Association of Zoos and Aquariums (AZA) to investigate the impact of integrating conservation into AZA members’ organizational cultures. Part of this work included AZA setting goals related to organizational and professional culture, strategic communication, developing communities of practice, and promoting tools and resource-sharing. Prior to implementing the majority of these steps, a baseline assessment was administered to directors plus a random sample of AZA organizations in 2020—assessing how well conservation is integrated into the institutional culture and measures of perceived conservation impact. The same sample of organizations was re-surveyed in 2023 with the intent of a three-year cycle of surveying to monitor change and identify additional ways that AZA could support and strengthen a culture of conservation within the profession. These findings will help the zoological and broader conservation community assess opportunities to integrate conservation into organizational cultures across a broad association for the purpose of achieving the mission and impact.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6030035

Authors: Arthur Silva Cristiano Azevedo Cynthia Cipreste Cristiane Pizzutto Eneida Eskinazi Sant’Anna

Food-based environmental enrichment (EE) is a valuable strategy for stimulating foraging behaviour in fish under human care, as it increases the challenge of food acquisition and encourages prolonged engagement in this activity. Curimbas (Prochilodus argenteus) and pacus (Myleus micans) are fish species for which ex situ maintenance has become an important conservation measure. In this context, providing EE is essential to ensure high welfare standards. This study aimed to assess the effects of food enrichment on the behaviour of these two endemic species from the São Francisco River basin in Brazil. Behavioural data were collected across three experimental phases, including baseline, enrichment, and post-enrichment. Slow-dissolving food items known as “acorns” were introduced during the enrichment phase. Both species exhibited a marked reduction in inactivity throughout the study. For curimbas, the enrichment phase was associated with increased foraging, elevated agonistic interactions, and greater use of specific tank areas. Among pacus, inactivity significantly declined during the enrichment period. Additionally, the presence of visitors influenced behavioural patterns, promoting foraging activity while reducing inactivity and interactions with the enrichment device. These findings reinforce the value of incorporating environmental enrichment to promote the welfare of freshwater fish in public aquariums.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020034

Authors: Marko Ivanov Kiril Valkanov Radoslav Tsvetkov Nikolay Natchev

In the present study, we examine the possibilities of planned generation in snakes by controlling the two most important factors for their estrus—ambient temperature and daylight hours. As a result of controlling these environmental parameters in an increasing gradient until reaching optimal values for the species, we observed copulations in late March and early April between a female Vipera ammodytes transcaucasiana and a male V. a. ammodytes. After three months of “pregnancy”, we obtained viable offspring in early July, which is about two months earlier compared to wild populations. The species used in the experimental setup, in natural conditions, usually produce offspring in late August to early October. Another aspect considered in the publication and followed in the experimental setup was to possibly test if in evolutionary and developmental aspect, both subspecies are closely related and interspecies breeding is possible (which might indicate mutual ancestry). The hybrid individuals were monitored during their entire development from newborns to subadults for pathological traits during development to roll-out crossbreeding incompatibility. In our pilot investigation, no acquired or inherited pathological traits have been observed. The individuals were consistent with feeding and exhibited excellent individual development. Future research coupled with genetic investigation can give valuable insight in the field, whether it is valid to regard the genera as a Vipera ammodytes complex or as different subspecies groups.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020033

Authors: Kaitlin Barrailler Cameron T. Whitley Sarah Brenkert Mary Jackson Nicole Killebrew Darcie Larson

Marine animals without faces (MAWFs), are some of the most important creatures maintaining the ecological balance in marine environments. How these animals are depicted across conservation organizations may impact public perceptions and conservation efforts. We assessed the online presentation of sea stars, jellies, and corals among all public websites of institutions accredited by the Association of Zoos and Aquariums (AZA) (N = 237). Among the organizations with an aquarium (n = 125), only 55 (44 percent) profiled at least one of the three animals, resulting in 89 total profiles. Five general approaches to characterizing these animals emerged: (1) scientific social distancing, (2) beautiful and eye-catching, (3) grotesque, otherworldly, and strange, (4) brainless beauties, and (5) objects of touch, entertainment, and experience. While some practices, like touch exhibits, can support empathy outcomes among visitors, online profile practices may contribute to the objectification of these animals among visitors, which could ultimately impact conservation attitudes, intentions, and behaviors.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020032

Authors: Paul Rose Xavier Manteca

The welfare of animals in zoos has come under increasing scrutiny as public awareness grows around the biological needs of captive species. It is also becoming clear that promoting positive welfare experiences upholds population management and conservation aims. This paper re-evaluates current welfare frameworks in zoological institutions, advocating for evidence-based practices, multi-dimensional welfare metrics, and greater emphasis on species-specific needs, as well as the importance of input-based approaches to assess the welfare of zoo animals. By evaluating the limitations of current welfare practices (e.g., a lack of species-specific assessment protocols or sound husbandry evidence to base measures on) and presenting potential areas for improvement, this paper identifies ways that sound baselines for meaningful zoo animal welfare outputs can be created. Although current welfare policies from large zoo membership organisations stipulate assessment of welfare outputs as key to improving animal welfare standards, such outputs can only be positive if inputs are species-specific and relevant to the animals being housed. Practices such as the use of environmental enrichment (for example) need to be further refined to ensure they provide meaningful outputs (for the individuals) from the inputs that create them. Understanding the animal’s needs to ensure that the goal of enrichment is clear benefits both the animal who is provided with the enrichment and the human caregivers as husbandry and management becomes easier. A focus on welfare outputs is commendable and (especially when considering emotional outputs) is indeed a gold standard to aim for, yet we must not lose sight of striving for improvements to housing, husbandry, and species-specific care. Without such fundamental support from correct inputs, outputs are unlikely to be truly (or meaningfully) positive. Therefore, consistent re-examination of inputs is required to make sure they uphold an individual’s attainment of good welfare.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020031

Authors: Luciana Diniz Rola Iara Maluf Tomazella Eluzai Dinai Pinto Sandoval Jorge Alfonso Morales-Donoso Carolina Heloisa de Souza Borges José Maurício Barbanti Duarte

Considering the taxonomic uncertainties of Neotropical deer species, as well as the threat status of many of them, new studies and strategies for their maintenance are urgently needed. Obtaining live cells is of great importance for the conservation of wild species in order to allow cytogenetic and molecular studies to be carried out and for the construction of genomic resource banks. In order to increase the genetic diversity stored in these banks, the possibility of collecting skin fragments from dead animals (e.g., run over, hunted, deaths related to disease or natural causes) becomes a valuable source and a last alternative for obtaining material from these individuals. However, the interval between the death of the animal and the collection of tissue can directly interfere with the quality of the sample obtained and it is therefore essential to identify the maximum time during which viable cells are still found. Thus, this study sought to establish a protocol for the collection, storage, cryopreservation, and cultivation of skin obtained postmortem from individuals of the species Subulo gouazoubira (gray brocket deer) and Mazama rufa (red brocket deer). The collection of tissue fragments at different postmortem intervals (0 h, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, and 11 h) was evaluated. The tissues were analyzed for fibroblast cell viability immediately after collection. Their ability to undergo cryopreservation was evaluated based on techniques that can be directly applied to samples obtained in the field and their subsequent thawing and success of cell cultures was performed in the laboratory. Regarding the genetic integrity of the cells, the number of metaphases was observed by the mitotic index. The cell viability presented by the samples always remained above 60%. It was possible to establish cell cultures even with the tissues obtained 11 h after the death of the individuals; however, they required twice as many days to reach bottle confluence compared to the cultures performed with the tissues obtained 0 h after the death of the individuals. The results suggest that the best rates of cell viability, time to reach confluence, and number of metaphases per cell (mitotic index) are found in skin fragments collected up to 5 h after the death of individuals when their carcasses are kept at room temperature.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020030

Authors: Ujjawala KC Shailendra Sharma Asmit Subba Naresh Pandey Ankit Kumar Singh Narayan Prasad Koju Laxman Khanal

Butterflies have nectar-feeding preferences based on various floral characteristics, including flower shape, size, color, fragrance, and nectar composition, which in turn affect their survival, reproduction, and roles in pollination. The National Botanical Garden (NBG) in Lalitpur, Nepal, holds a variety of flowering plants and butterfly populations, providing a suitable study site to test the hypotheses on floral preferences of butterflies. This study assessed the floral preferences of the butterfly community in the NBG based on flower color, the origin of flowering plants (native and alien), and the type of plants (herbs and shrubs). It also tested the association between butterfly proboscis lengths and corolla tube lengths of flowers. Data were collected from 10 blocks (each 5 × 5 m2) through direct observation during the spring and autumn seasons, from March to October 2022. A total of 24 species of butterflies were recorded during the study period, with the chocolate pansy (Junonia iphita) being the most abundant. The relative abundance of pink flowers was higher in the NBG, but the butterflies’ visitation frequency was significantly higher on yellow flowers (p < 0.05) than on other colors. The visitation frequencies of butterflies significantly varied with the flowers’ origin and types. Butterflies visited flowers of alien origin more frequently than native ones (p < 0.05) and those of herbs over shrubs (p < 0.05). Flowers from alien plants, such as Calluna vulgaris and Viola tricolor, were among the most frequently visited. The proboscis length of butterflies showed a significantly strong positive correlation with the corolla tube length of flowers (τ = 0.74, p < 0.001). These results can inform conservation practices and garden management strategies aimed at supporting butterfly diversity through the intentional selection of floral resources.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020029

Authors: Jon Coe James Edward Brereton Eduardo Jose Fernandez

Zoo, aquarium, and sanctuary exhibit designers, both specialist “zoo architects”, and general practice architects, as well as landscape architects generally do not closely follow the evolving scientific literature on zoo biology, visitor experience, and managed animal welfare. Reportedly, this is because most zoo and aquarium clients do not require these efforts. Detailed requirements are provided by clients as project programs or briefs, which vary widely in quality and currency. Many clients and designers copy or adapt popular enclosure models without regard to their scientific foundations. Research papers frequently focus on discrete subject areas, such as animal behavior and welfare, visitor experience, or education, using their own methods and vocabulary. Relatively few studies integrate findings in ways useful to designers in preparing widely integrated systems. Regulatory standards set minimum rather than ideal standards. Knowledge of in situ animal behavior is lacking for many managed species. How can zoo and aquarium managers and designers be encouraged to increase research within the design process? This review article suggests that the long-term benefits of greater and better science integration outweigh initially higher design costs, resulting in improved facility and management design, benefiting all zoo, aquarium, and sanctuary stakeholders, and providing factual evidence underpinning community support.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020028

Authors: Lorena Esteban-Sánchez Marta Mateo-Barrientos Manuel de la Riva-Fraga Lino Pérez de Quadros Juan José García Rodríguez Francisco Ponce-Gordo

Flagellates of the genus Giardia are intestinal parasites with a broad host range. Several Giardia duodenalis variants (assemblages) recently elevated to species rank—G. duodenalis (assemblage A1), G. intestinalis (A2) and Giardia enterica (B) are human pathogens. Giardia enterica has been reported in some hystricomorph rodents such as wild crested porcupines (Hystrix cristata), but no data were previously available from Brazilian porcupines (Coendou prehensilis) and naked mole rats (Heterocephalus glaber). The aim of this study is to genetically identify the Giardia isolates from these three rodent species, all housed in a zoological institution. Fecal samples were processed using the Bailenger concentration method, and DNA was extracted from the sediments using commercial kits. Partial PCR amplification and sequencing of the glutamate dehydrogenase, beta-giardin, and triose-phosphate isomerase genes revealed that all isolates belonged to G. enterica, showing 99–100% identity with sequences available in GenBank. Prevalences could not be reliably estimated due to small group sizes and the resulting proportions may be biased. To our knowledge, this is the first report identifying Giardia (G. enterica) in C. prehensilis and H. glaber, thus expanding the known host range of this parasite species and reinforcing the importance of surveillance in captive wild hosts.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020027

Authors: Lina Podda Andrea Lallai Giacomo Calvia Francesco Mascia Gianluca Iiriti Gianluigi Bacchetta

Botanical gardens have historically introduced alien species for agronomic, medicinal, and ornamental purposes, but they also contribute to plant invasions. The Hortus Botanicus Karalitanus (HBK) is a historic botanical garden established in 1866, as an acclimatisation arboretum for tropical plants, by the University of Cagliari. This study inventoried alien vascular plants in the HBK that showed some degree of spontaneity, analysing their status, origin, life form, introduction pathways, reproductive strategies, and presence in Sardinian habitats. A focus on invasive species and their historical introduction based on their first records on the island and in the HBK was made. Field surveys from 2015 to 2024 allowed us to identify 146 alien taxa, primarily neophytes (83%), 45% of which were naturalised and 12% were invasive. Ornamental plants contributed to 70% of introductions, while accidental taxa (weeds and hitchhikers, 61%) were the most invasive. Seed reproduction was the most common way of propagation (41%). About the diffusion in Sardinia, 25% of taxa had no records in nature outside the HBK, 39% were found only in anthropogenic habitats, and 36% in natural habitats. Among them, 8% were invasive in both the HBK and Sardinia, with wetlands being the most affected (5%). However, only 3% of common invasive species were first recorded in the HBK. The results highlight the role of the HBK in alien species spread and early detection, aiding in invasion management and biodiversity conservation in Sardinia.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020026

Authors: Janna Akopian Anahit Ghukasyan Araksya Elbakyan Lora Martirosyan Zhanna Hovakimyan

Amberboa moschata (L.) DC. (Asteraceae) is an endangered species, listed in the Red Book of Plants of the Republic of Armenia. The restricted extent of occurrence and habitat degradation necessitate conservation measures of this species, not only in the wild but also through ex situ cultivation. This study examines the comprehensive morpho-phenological, karyological, palynological, eco-physiological, and ornamental characteristics of A. moschata in the context of ex situ conservation. A. moschata plants cultivated ex situ demonstrated high adaptive traits, undergoing a full development cycle and experiencing less water stress compared to wild populations. The diploid cytotype has been found for the species to be 2n = 32, the karyotype is asymmetric, with chromosomes, 0.77–1.91 µm in size. The average pollen fertility of A. moschata is high, 96.7–96.9% in both natural and cultivated samples. A scale of decorativeness was developed, which includes 15 characteristics of the plant, providing an objective means to assess its visual appeal. The scale can be useful for integrating A. moschata into various landscaping schemes. Under cultivation, the total ornamental period lasts approximately 98 days, with the peak ornamental effect observed during the flowering phase, which spans 68–70 days. The study recommends A. moschata for inclusion in living collections in botanical gardens and for use in ornamental gardening and landscaping as part of its ex situ conservation strategy. A map, original photographs, and tables illustrate the article.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020025

Authors: Peggy Cremers Max Norman Sabrina Brando Eduardo J. Fernandez

Ongoing research on the effectiveness of enrichment at the species, group, and individual levels is beneficial to our growing understanding of animal welfare and behaviour for animals housed in human care. Diversifying the enrichment opportunities offered to animals in facilities such as zoos and aquariums helps to encourage a wide repertoire of species-typical, naturalistic, and rewarding behaviours. The present study aimed to examine the behavioural impact of novel food (ice blocks, gelatine, eggs) and non-food (lavender, clean sheets, mirrors) enrichment strategies on two zoo-housed prides of African lions (Panthera leo) housed in a rotation-based enclosure system at Orana Wildlife Park, New Zealand. The results of the study indicate that, while both forms of enrichment had a behavioural impact, the effects on behaviour varied with the type of enrichment used, both between and within the two categories. Different enrichment strategies varied in their impacts on resting behaviour, locomotion, and exploration levels in the lions. An increased focus on the specific behavioural outcomes of various novel enrichment strategies is essential to expand the efficacy of enrichment programs for future efforts to improve the wellbeing of zoo-housed animals.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020024

Authors: Sinh Hoang Nguyen

This study investigates how virtual reality (VR) experiences influence tourists’ intentions to visit Da Lat, Vietnam, as a botanical destination, emphasizing the mediating roles of enjoyment and immersion. By integrating flow theory with the Information Systems Success model, this research develops a comprehensive framework explaining how content quality, system quality, and VR vividness shape user engagement and travel intentions. Using Structural Equation Modeling (SEM), the study analyzes survey data from 231 valid responses out of 240 participants. The findings reveal that content quality, system quality, and vividness significantly enhance enjoyment and immersion, which subsequently have a positive impact on travel intentions. The study contributes to the tourism and consumer experience literature by demonstrating how multisensory engagement in VR fosters decision-making. Theoretical implications include extending flow theory within virtual tourism and highlighting the joint influence of technological and perceptual factors on user behavior. Practically, these insights inform tourism marketers on optimizing VR environments to evoke emotional engagement and enhance destination appeal through immersive technology.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020023

Authors: Chiara Carpino Roberta Castiglioni Elisa Sacchet Arianna Milesi Luca Marano Francesco Luigi Leonetti Viviana Romano Gianni Giglio Emilio Sperone

For the effective management of captive animals, monitoring their behavior and interactions within the exhibit is essential. This approach provides insights into their welfare and helps identify behavioral differences compared to conspecifics in the wild. This ex situ study aimed to provide more insights about the behavior of a pair of siamangs residing at the Wildlife Park “Le Cornelle” in Valbrembo, Italy. The focus was on their behavior, and observations were conducted during two distinct periods (May–June 2021 and December 2021) to identify any seasonal differences. In both periods, the most frequent behaviors observed were resting, feeding, and allogrooming. Compared to conspecifics in the wild, allogrooming and singing occurred more frequently. Spatial analysis indicated a higher utilization of outdoor spaces than the tunnel and indoor areas, with statistically significant variations for both periods. The spatial analysis of singing behavior showed an equal use of outdoor and indoor areas during the first observation period. However, in the second observation period, a preference for indoor spaces was observed, likely due to lower recorded temperatures. More observations are needed to identify the factors influencing behavior, but it is clear that continuous monitoring plays a vital role in promptly addressing deviations or anomalies in animal behavior.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020022

Authors: Max Norman Sabrina Brando

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Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020021

Authors: Alexandra Vaccaro Jill Korach

It is surprising that, despite focusing on delivering engaging and informative content to zoo and aquarium visitors nationwide, relatively few zoos or aquariums incorporate culture or the extensive species and place-based knowledge of local communities, also known as traditional ecological knowledge (or TEK). In an exploratory study of educational programming at the Wildlife Conservation Society’s five zoo and aquarium institutions, we found that only 15% of onsite signage, webpages, and in-person animal programs used cultural or TEK elements to educate visitors. The TEK presented was primarily limited to a place or cultural group’s name only or an image of native communities who live in the region where a particular species originated. We found little to no focus on providing more detailed accounts of the knowledge of the many different communities contributing to conservation at a local level. We conclude there are many opportunities for further research and for informal education facilitates to readily incorporate culture and TEK into existing exhibits and programming. Extending relationships with local native community members and integrating indigenous perspectives, among other strategies, can extend a zoo’s reach in novel ways.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6020020

Authors: Mariana Panayotova-Pencheva Joanna Banasiewicz Anna Maria Pyziel

Faecal parasitological examination of three red kangaroos (Osphranter rufus) newly arrived at Sofia Zoo, Bulgaria, revealed infestation with nematode eggs. The animals were successfully treated with albendazole and ivermectin. Adult nematodes obtained after deworming the kangaroos were identified as gastric strongylids of the species Rugopharynx australis (Mönnig, 1926). The initially collected faecal samples were used to follow the development of the parasites, in particular, the viability of the larvae hatching from the eggs. Morphometric and molecular data as well as some epidemiological observations of the species in the present materials are provided. The study provides the first morphological description of the free-living, infective third-stage larvae of the species. It is also the first record of R. australis in captive-bred kangaroos on the European continent.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010019

Authors: James Edward Brereton Jon Coe Eduardo J. Fernandez

Good enclosure design is central to the improvement of conditions for animals housed in zoos and aquaria, yet the study of a priori enclosure design or post hoc through Post-Occupancy Evaluation (POE) is somewhat limited in the scientific literature. The concept of trialing novel exhibit components before enclosure construction, through a process known as Temporary Exhibit Design (TED), was recently proposed as a strategy to prevent problems that often occur as a result of untested exhibit creation, with a view to ultimately improve welfare outcomes and reduce subsequent exhibit redevelopment. In this paper, we consider the potential input required from three key enclosure design stakeholders: the animal, the visitor, and zoo staff. We also consider the potential benefits for each of these stakeholders when participating in TED, alongside the wider zoo organizational benefits. TED has the potential to reduce construction costs and post-construction redevelopment, enhance animal welfare, and ensure that educational messages are effectively communicated.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010018

Authors: María Elizabeth Bejarano Meza Gianela Milagros Paz Cahuina Paola Fabiana Vásquez Macedo Andrea Marieta Chanove Manrique

Arequipa, situated in the arid Atacama Desert, is experiencing significant biodiversity loss due to unchecked urban growth. This study presents the design of a Botanical Garden to address this issue by serving as a conservation center for native flora. A baseline assessment was conducted to evaluate meteorological conditions, soil properties, and the quality of treated wastewater from the “La Escalerilla” plant. The analysis revealed a semi-arid climate with low precipitation and suitable soil conditions. A total of 685 plant species were identified and proposed for conservation within the garden, with a high proportion of endemic species. To protect the future plant species, a living fence composed of 700 individuals was installed, considering resilient species such as Molle serrano (Schinus molle), Cantuta (Cantua buxifolia), and Huaranguillo (Tephrosia cinerea), which were installed at an average height of 50 cm and have grown to an average of 80 cm after 8 months. This study demonstrates the feasibility of establishing a botanical garden in Arequipa’s arid environment and serves as a model for future botanical garden projects in arid regions at the national and regional levels, providing a foundation for natural landscapes in urban areas.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010017

Authors: Francesca Milan Serena Lucrezi Freisha Patel

Human activities significantly contribute to the biodiversity crisis, yet wildlife tourism can promote appreciation for animals and encourage conservation. This study assessed the impact of diving and snorkeling programs at Atlantis Dubai on tourists’ emotional connections with marine life and their conservation attitudes and pro-environmental behavioral intentions. In 2023 and 2024, 346 participants were surveyed before (n = 172) and after (n = 174) their experiences diving and snorkeling with elasmobranchs. Results indicated a notable increase in positive feelings toward sharks and rays, strengthening connections and respect for these creatures. The program positively influenced attitude toward sharks, making participants more inclined to support their protection and foster a deeper connection with the ocean. Although participants’ willingness to recycle improved, overall conservation attitudes and willingness to engage in broader pro-environmental behaviors showed minor change. However, the heightened positive emotions towards sharks resulted in improved perceptions of these animals, stronger conservation attitudes, and a commitment to environmental sustainability. A deeper connection with the ocean reduced fear of sharks. This research suggests that wildlife tourism can facilitate emotional connections with marine life, potentially inspiring conservation action and highlighting the need for targeted strategies to transform these emotional bonds into lasting pro-environmental behaviors.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010016

Authors: Margaret Ramont Cody Hickman Tim Snyder Sathya K. Chinnadurai Lance J. Miller

Until recently, the sihek (Guam kingfisher, Todiramphus cinnamominus) has persisted entirely in managed care since a breeding program was established in the 1980s. Understanding the growth and developmental milestones of the sihek from hatch to fledging is critical to enhancing conservation efforts and improving management practices that promote positive welfare. In this study, we summarized data collected on the growth patterns, developmental milestones, and rearing differences in sihek chicks raised at Brookfield Zoo Chicago from 1989 to 2023. We found that hand-reared chicks reached the fledging stage significantly earlier than parent-reared chicks that received supplemental feeding from staff. Additionally, we found that hatch weight was a significant predictor of survival to fledging, highlighting the importance of collecting weight data at all stages of life. These insights into sihek development in managed care provide important data for the development of welfare-focused management practices that improve the success of conservation efforts while also prioritizing the wellbeing of each individual chick.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010015

Authors: Dražen Đuričić Maja Lukač

Numerous previous studies indicate that both wild hedgehogs and those kept as pets pose a significant risk to human health. At the same time, humans can also transmit various pathogens to hedgehogs (e.g., human herpesvirus 1). The risk of human infection from hedgehogs by various bacteria, viruses, fungi, rickettsiae, and parasites, and of hedgehog infection from humans, arises from close contact with pet hedgehogs, wild hedgehogs, and/or the contaminated environment which they cohabit with humans. People can also come into close contact with hedgehogs in city parks, rescue and rehabilitation centers, gardens, suburbs, and zoos. Numerous zoos keep different species of hedgehogs, which are often used for education and interaction with visitors, especially children. In spite of certain preventive measures, periodic examinations, and disease controls being carried out, the possibility of the transmission of infectious diseases from hedgehogs to visitors and employees is not excluded. Close contact poses a risk, especially if biosecurity is not properly maintained. The anthropological effects of coexistence, habitat sharing, and frequent human intrusion into hedgehog habitats have disrupted the natural relationship between humans and animals, and have led to an increase in the prevalence of various pathogens. Although many different pathogens have been isolated in hedgehogs, there is a need to study some of them in more detail and to understand their interaction and transmission possibilities, as well as zoonotic and reverse zoonotic pathogens.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010014

Authors: Florin Crișan Dan Gafta Irina Goia

The present study is based on a follow-up of a survey carried out in 2000, consisting in the revisitation of ten sites, with the scope of assessing changes in the composition and richness of epiphytic macrolichens within Cluj-Napoca city over the past 24 years. Within this period most of the polluting factories from the city outskirts were closed but in turn, the number of registered cars increased almost six-fold. An increasing compositional homogenization by contribution of generalist, stress-tolerant species was detected over time while total lichen taxa richness declined, which is mostly imputable to the synergic effects of intense car traffic and warmer/drier summers. Most sites displayed a compositional change along a weak, mixed gradient of eutrophication and xerophitization. Only two sites (located on the windy, Someș valley bottom) experienced a compositional change from higher to lower trophicity levels. Other two sites (positioned on more sheltered hillsides) displayed unfavourable dynamics in terms of lost species. Unexpectedly, the number of epiphytic lichen taxa at site level has, on average, increased over time, but the main contributors were poleotolerant species. The warming trend, due to climate change and local heat sources, is expected to worsen the negative effects of air-borne pollutants on the composition of the epiphytic lichen species pool within the Cluj-Napoca urban area.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010013

Authors: Murillo Daparé Kirnew Matheus Folgearini Silveira Roberto Andres Navarrete Ampuero Ana Paula Rodrigues Simões Felippe Azzolini Gediendson Ribeiro de Araújo Pedro Nacib Jorge-Neto Sofia Regina Polizelle Juliane Patrícia Sipp Cristiane Schilbach Pizzutto Thyara Deco-Souza Aparecido Antonio Camacho

Jaguars play a crucial role in population control across multiple biomes. They are endangered and protected by in situ and ex situ conservation mechanisms to ensure their conservation. Cardiovascular diseases in wild mammals, including jaguars, often have unclear etiopathogenies, underscoring the need for research into novel hemodynamic parameters. This study evaluates the cardiovascular health of fifteen clinically healthy jaguars using conventional and Holter electrocardiography, non-invasive systemic blood pressure measurement, and echocardiography. Chemical restraint was achieved with medetomidine (0.08–0.1 mg/kg) and ketamine (5 mg/kg), with anesthesia reversed using atipamezole (0.25 mg/kg). The average heart rate was 72 ± 18 bpm, with sinus rhythm in ten animals and sinus arrhythmia in five. Six animals exhibited first and second-degree atrioventricular blocks, one had supraventricular complexes, and another had premature ventricular complexes. Non-invasive systolic blood pressure remained stable at 163 ± 29 mmHg during anesthesia. Echocardiographic examination revealed mitral, tricuspid, pulmonary, and aortic valve insufficiencies via color Doppler. The transmitral flow showed a normal E/A ratio and E` < A`, suggesting a pseudonormal ventricular filling pattern. No significant anesthetic complications were observed, affirming the protocol’s safety. This study provides valuable data, validating the anesthetic protocol and establishing reference cardiovascular values for jaguars, thus paving the way for future research in other veterinary species.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010012

Authors: David Dantas Cláudia Luís Batista Maria João Castro Nuno Alvura Teresa Letra Mateus

The growing popularity of reptiles has contributed to their reproduction in captivity. When subjected to stressful environments, such as the presence of a higher number of humans and animals, reptiles may become more susceptible to parasites. Endoparasites in captive animals may cause several clinical signs ranging from mild to severe: lethargy, anorexia, diarrhea, cloacal/penile prolapse, infertility, intestinal malabsorption syndrome, and weight loss, among others. This study aimed to assess the presence of gastrointestinal parasites in fecal samples of reptiles from a Portuguese zoo through two techniques: a fecal flotation test (using a saturated sodium chloride solution) and Mini-FLOTAC. Ninety-nine samples belonging to 22 different animal species were collected and analyzed. Parasites were identified in 53.5% of the samples. Chelonians had a higher frequency (100%), followed by lizards (56.8%) and snakes (47.4%). The eggs/oocysts found were oxyurids (36.4%), strongylids/Kalicephalus sp. (8.1%), Eimeria sp. (5.1%), Hymenolepis spp. (5.1%), ascarids (4.0%), and Isospora sp. (2.0%). Both techniques presented the same results for each sample. The high prevalence of oxyurids, as well as of other parasites, can be explained by possible environmental contamination as these reptiles are kept in captivity. This study indicated the importance of assessing parasitic infections in reptiles in zoos, where routine coprological examinations should always be considered, as well as adequate prophylaxis.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010011

Authors: Szabolcs Szanyi Csenge Lelle Kovács Miklós Tóth Ottó Lincz Gergely Sándor Nagy Zoltán Varga Antal Nagy

Zoos and botanical gardens have a special role in the promotion, presentation and conservation of biodiversity in urbanised environments. Additionally, they provide special habitats for alien and invasive species. The formerly used methods of biodiversity assessments (e.g., light trapping, transect counts, etc.) are mostly labour-intensive and/or not efficient enough. In the Zoo of Debrecen, the efficacy and suitability of a synthetic (FLO) and a semisynthetic (SBL) lure for this purpose were proven. The qualitative and quantitative compositions of a moderately rich moth assemblage including 52 moth species were revealed, and the appearance and population dynamics of three invasive (Helicoverpa armigera, Autographa gamma and Cydalima pespectalis) and nine harmful pest species were also recorded. The results proved that the lures tested and traps used provide an easy-to-use, standardised and relatively cheap method for Zoos and botanical gardens to assess their biodiversity even in the case of limited resources.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010010

Authors: Kees Groot Paul Rose

Tropical forest hornbills display complex social and reproductive behaviours that make them hard to reliably and frequently breed in ex situ facilities. This study investigated potential behavioural indicators of pair compatibility for two wreathed hornbills (Rhyticeros undulatus) at ARTIS Amsterdam Royal Zoo. We focused on behaviours linked to reproductive investment, such as time spent in proximity, vocalisation, and courtship display. Through systematic observations using ZooMonitor, we recorded behavioural and enclosure use data, as well as visitor presence. Key findings revealed that the male hornbill was more active and exploratory than the female. Courtship behaviours were more frequently performed by the male bird and were positively associated with perching at elevated positions. However, this behaviour decreased when the birds were in close proximity or inactive. Visitor presence influenced enclosure use, with the hornbills preferring higher perches during peak visitor times. We conclude that behaviours such as proximity, vocalisations, and courtship displays could potentially serve as indicators of compatibility in hornbill pairs but more data across more birds are needed for validation. Finally, we advocate for enclosure designs that consider hornbills’ natural behaviours and social needs to help ex situ facilities promote an environment conducive to pair bonding.

Journal of Zoological and Botanical Gardens doi: 10.3390/jzbg6010009

Authors: Claire Lannoye-Hall Patricia G. Patrick

We applied social cognitive career theory and systems theory to explore the barriers that historically excluded groups face when pursuing animal care careers at U.S. zoos and aquariums. Using a mixed-methods approach, this research combines survey data (N = 592) and interviews (N = 15) from zoo and aquarium animal care staff, including zookeepers, veterinarians, and aquarists. The findings show that historically excluded groups of animal caretakers report higher levels of perceived barriers to securing zoo positions compared to White animal caretakers, particularly in career awareness, self-efficacy, mentorship, and access to internship opportunities. Statistical analyses of animal caretakers revealed a statistically significant difference between historically excluded groups and their White colleagues. Historically excluded groups experience more challenges in securing and succeeding in animal care careers. While institutional barriers did not differ significantly between groups, this study underscores the need for zoos to address systemic inequities and create more inclusive environments. These findings are critical for zoos aiming to diversify their staff and enrich their decision-making processes.