Evaluation of the Safety of Immobilized Microorganisms Lysobacter sp. on Inorganic Media †
Abstract
:1. Introduction
2. Materials and Methods
- Sodium carboxymethyl cellulose (NaCMC) technical brand “KMC 85/500” produced by LLC “Davos-Trading”. TU 2231-001-53535770-2010 (with change No. 1.2): degree of substitution for carboxymethyl groups 80–90; degree of polymerization 500–550; pH value (pH) of an aqueous solution with a mass fraction of CMC 1% in the range of 8–12; dynamic viscosity of a 2% CMC solution at a temperature of 25 °C mPa*s according to the Brookfield method, not less than 100.
- Colloidal silicon dioxide in the form of a commercial preparation called “Polysorb,” produced by JSC “Polysorb.” Polysorb MP (medical oral) is an inorganic, non-selective, multifunctional enterosorbent based on highly dispersed silica with particle sizes up to 0.09 mm and the chemical formula SiO2. The sorption capacity of the drug for internal use is 300 m2/g.
- Sodium form of montmorillonite (NaMMT) from the Podgorenskoye deposit, Voronezh region [4,5], obtained by introducing soda ash (4 wt%) into a native rock suspension (5 wt%), followed by sedimentation enrichment and drying (t = 95 ± 3 °C). The quantitative content of montmorillonite, determined according to GOST 28177-89—79.35 ± 0.14 wt.%. As a result of the modification, the crystal lattice parameters changed: for the native form of Ca-montmorillonite, a = 5.16 Å, b = 8.94 Å, c = 15.02 Å; for modified montmorillonite, a = 5.22 Å, b = 9.04 Å, c = 13.82 Å. Specific surface, 60 m2/g, specific pore volume, 0.083 cm3/g; average pore size, 55.5 Å.
3. Results
- Lyophilization of the bacterial culture of Lysobacter sp. without immobilization on the matrix leads to a 37.5% decrease in safety;
- Cell immobilization on sodium carboxymethyl cellulose allows safety to increase up to 65%, and on colloidal silicon dioxide (Polysorb), it increases up to 50%;
- When immobilized on the mineral montmorillonite, not only is the preservation of microorganisms manifested, but there is also an 18% increase in the number of cells.
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Biomass/Carrier Ratio | CFU * (g/L) after Storage | |||
---|---|---|---|---|
Day 2 | Day 15 | Day 31 | Day 92 | |
Freeze culture | 2.4 ± 0.04 × 105 | 2.3 ± 0.06 × 105 | 2.0 ± 0.04 × 105 | 0.9 ± 0.05 × 105 |
NaCMC 1:2 | 3.4 ± 0.06 × 105 | 3.7 ± 0.02 × 105 | 3.9 ± 0.05 × 105 | 2.1 ± 0.02 × 105 |
NaCMC 1:3 | 3.5 ± 0.04 × 105 | 3.8 ± 0.03 × 105 | 4.1 ± 0.07 × 105 | 2.7 ± 0.07 × 105 |
NaCMC 1:4 | 3.3 ± 0.07 × 105 | 3.5 ± 0.03 × 105 | 3.6 ± 0.05 × 105 | 1.9 ± 0.03 × 105 |
Polysorb 1:2 | 3.5 ± 0.02 × 105 | 3.2 ± 0.02 × 105 | 2.7 ± 0.01 × 105 | 1.8 ± 0.02 × 105 |
Polysorb 1:3 | 3.2 ± 0.03 × 105 | 3.1 ± 0.01 × 105 | 2.5 ± 0.05 × 105 | 1.7 ± 0.01 × 105 |
Polysorb 1:4 | 3.3 ± 0.03 × 105 | 3.1 ± 0.05 × 105 | 2.4 ± 0.07 × 105 | 1.5 ± 0.04 × 105 |
NaMMT 1:2 | 3.6 ± 0.03 × 105 | 3.8 ± 0.02 × 105 | 4.0 ± 0.04 × 105 | 4.2 ± 0.06 × 105 |
NaMMT 1:3 | 3.5 ± 0.05 × 105 | 3.7 ± 0.06 × 105 | 3.9 ± 0.07 × 105 | 4.1 ± 0.03 × 105 |
NaMMT 1:4 | 3.4 ± 0.01 × 105 | 3.8 ± 0.04 × 105 | 4.0 ± 0.06 × 105 | 4.1 ± 0.03 × 105 |
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Shaidorova, G.; Vesentsev, A.; Krut, U.; Kuzubova, E.; Radchenko, A.; Potapova, M. Evaluation of the Safety of Immobilized Microorganisms Lysobacter sp. on Inorganic Media. Eng. Proc. 2023, 37, 71. https://doi.org/10.3390/ECP2023-14706
Shaidorova G, Vesentsev A, Krut U, Kuzubova E, Radchenko A, Potapova M. Evaluation of the Safety of Immobilized Microorganisms Lysobacter sp. on Inorganic Media. Engineering Proceedings. 2023; 37(1):71. https://doi.org/10.3390/ECP2023-14706
Chicago/Turabian StyleShaidorova, Galina, Alexander Vesentsev, Ulyana Krut, Elena Kuzubova, Alexandra Radchenko, and Marina Potapova. 2023. "Evaluation of the Safety of Immobilized Microorganisms Lysobacter sp. on Inorganic Media" Engineering Proceedings 37, no. 1: 71. https://doi.org/10.3390/ECP2023-14706