Next Article in Journal
Non-Invasive IR-Based Measurement of Human Blood Glucose
Previous Article in Journal
In Silico Analysis of Toehold-Aptamer Sequences Targeting the SARS-CoV-2 Nucleocapsid Protein Gene for Biosensor Development
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Engineering Proceedings
Volume 35
Issue 1
10.3390/IECB2023-14595
engproc-logo
Submit to this Journal
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Proceeding Paper

Simple Chromatographic Sensor with UV LED Optical Detection for Monitoring Patients Treated with Continuous Ambulatory Peritoneal Dialysis †

by
Georgii Konoplev
1,*,
Artur Kuznetsov
2,
Aleksandr Frorip
2,
Alar Sünter
2,3,
Vadim Korsakov
4,
Oksana Stepanova
1,
Natalia Roschina
1,
Nikolay Ovsyannikov
1,
Roman Gerasimchuk
5,
Alina Isachkina
6,
Zarina Rustamova
6 and
Alena Pavshukova
7
1
Department of Photonics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, Russia
2
AS Ldiamon, 50411 Tartu, Estonia
3
Chair of Veterinary Biomedicine and Food Hygiene, Estonian University of Life Sciences, 51006 Tartu, Estonia
4
Jeko Disain OÜ, 51014 Tartu, Estonia
5
Dialysis Unit, Saint Petersburg City Mariinsky Hospital, 191014 Saint Petersburg, Russia
6
Chair of Internal Diseases, Clinical Pharmacology and Nephrology, North-Western State Medical University named after I.I. Mechnikov, 191015 Saint Petersburg, Russia
7
Laboratory of Neuroecology, Federal State Budgetary Scientific Institution “Institute of Experimental Medicine” (FSBSI “IEM”), 197376 Saint Petersburg, Russia
*
Author to whom correspondence should be addressed.
†
Presented at the 3rd International Electronic Conference on Biosensors, 8–21 May 2023; Available online: https://iecb2023.sciforum.net.
Eng. Proc. 2023, 35(1), 25; https://doi.org/10.3390/IECB2023-14595
Published: 9 June 2023
(This article belongs to the Proceedings of The 3rd International Electronic Conference on Biosensors)
Browse Figures
Versions Notes

Abstract

A novel simple optical sensor based on fast protein liquid chromatography was developed and tested for monitoring end stage renal disease (ESRD) patients treated with continuous ambulatory peritoneal dialysis (CAPD). The device provides direct determination of proteins and lower molecular weight metabolites in effluent peritoneal dialysate using ultraviolet (UV) photometric detection at the wavelengths 285 nm or 260 nm with deep ultraviolet light-emitting diodes. The sensor was calibrated with bovine serum albumin and nucleotides standard solutions. Chromatograms of peritoneal dialysate samples taken from a group of 28 ESRD patients were processed and approximated by a set of split-Gaussian functions. All chromatograms show three overlapping peaks: the first one represents proteins; the other two peaks probably correspond to mid- and low molecular weight metabolites. Strong correlation was reveled between the area of the first peak and total protein concentration determined by a standard biochemical assay, this makes possible estimation of peritoneal protein loss with a reasonable precision less than 15%. The area of the second peak correlated with dialysate optical density at a wavelength 355–365 nm, associated with the UV absorption of advanced glycation end (AGE) products. The third peak correlated with the optical density of the eluate at a wavelength 255–265 nm, associated with the UV absorption of purines and pyrimidines. Thus, we demonstrated the possibility of estimation of proteins and lower molecular weight metabolites in effluent peritoneal dialysate with the compact and affordable chromatographic optical sensor.
Keywords: optical chemical sensor; fast-protein liquid chromatography; UV LED; peritoneal dialysis; end stage renal disease; peritoneal protein loss optical chemical sensor; fast-protein liquid chromatography; UV LED; peritoneal dialysis; end stage renal disease; peritoneal protein loss

Share and Cite

MDPI and ACS Style

Konoplev, G.; Kuznetsov, A.; Frorip, A.; Sünter, A.; Korsakov, V.; Stepanova, O.; Roschina, N.; Ovsyannikov, N.; Gerasimchuk, R.; Isachkina, A.; et al. Simple Chromatographic Sensor with UV LED Optical Detection for Monitoring Patients Treated with Continuous Ambulatory Peritoneal Dialysis. Eng. Proc. 2023, 35, 25. https://doi.org/10.3390/IECB2023-14595

AMA Style

Konoplev G, Kuznetsov A, Frorip A, Sünter A, Korsakov V, Stepanova O, Roschina N, Ovsyannikov N, Gerasimchuk R, Isachkina A, et al. Simple Chromatographic Sensor with UV LED Optical Detection for Monitoring Patients Treated with Continuous Ambulatory Peritoneal Dialysis. Engineering Proceedings. 2023; 35(1):25. https://doi.org/10.3390/IECB2023-14595

Chicago/Turabian Style

Konoplev, Georgii, Artur Kuznetsov, Aleksandr Frorip, Alar Sünter, Vadim Korsakov, Oksana Stepanova, Natalia Roschina, Nikolay Ovsyannikov, Roman Gerasimchuk, Alina Isachkina, and et al. 2023. "Simple Chromatographic Sensor with UV LED Optical Detection for Monitoring Patients Treated with Continuous Ambulatory Peritoneal Dialysis" Engineering Proceedings 35, no. 1: 25. https://doi.org/10.3390/IECB2023-14595

APA Style

Konoplev, G., Kuznetsov, A., Frorip, A., Sünter, A., Korsakov, V., Stepanova, O., Roschina, N., Ovsyannikov, N., Gerasimchuk, R., Isachkina, A., Rustamova, Z., & Pavshukova, A. (2023). Simple Chromatographic Sensor with UV LED Optical Detection for Monitoring Patients Treated with Continuous Ambulatory Peritoneal Dialysis. Engineering Proceedings, 35(1), 25. https://doi.org/10.3390/IECB2023-14595

Article Metrics

Back to TopTop