Next Article in Journal
Molecularly Imprinted Polymer Based Capacitive Chemosensor for Determination of Heterocyclic Aromatic Amines in Food Samples
Previous Article in Journal
Design and Development of a Miniature Mid-IR Spectrometer for Environmental Sensing and Food Safety Applications
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Engineering Proceedings
Volume 21
Issue 1
10.3390/engproc2022021050
engproc-logo
Submit to this Journal
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

A Microsystem for the Study of Vascularization †

by
Joanna Konopka
1,*,
Patrycja Staniszewska
1,
Agnieszka Żuchowska
1 and
Elżbieta Jastrzębska
1,2,*
1
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664 Warsaw, Poland
2
Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822 Warsaw, Poland
*
Authors to whom correspondence should be addressed.
†
Presented at the 9th International Symposium on Sensor Science, Warsaw, Poland, 20–22 June 2022.
Eng. Proc. 2022, 21(1), 50; https://doi.org/10.3390/engproc2022021050
Published: 6 September 2022
(This article belongs to the Proceedings of The 9th International Symposium on Sensor Science)
Versions Notes

Angiogenesis is the development of new blood vessels from the already existing vasculature [1]. Its course is controlled by many pro- and anti-angiogenic factors. Disorders of the distribution of these agents lead to the development of cancer and cardiovascular diseases qualified by the World Health Organization as the leading causes of death globally. Understanding the mechanisms regulating physiological and pathological angiogenesis will allow for more effective treatment. The simulation of angiogenesis under microflow conditions is a promising alternative to studies on a 2D cell culture [2].
The microsystem consists of two layers. The upper layer contains a mapped network of channels. It is made of PDMS with the use of a micro-milled stamp. The bottom layer (a microscope slide) acts as a seal. The design of the microchip includes three parallel microchannels. Cylindrical microchannels seeded by Human Umbilical Vein Endothelial Cells (HUVECs) were generated using the viscous-finger patterning technique in the two lateral microchannels. Their diameter corresponds to the size of human arteries (701.5 ± 3.1 µm). The ultimate procedure will enable the reconstruction of narrower blood vessels, such as veins and capillaries. The middle microchannel allows for the introduction of a pro-angiogenic factor solution. The influence of Vascular Endothelial Growth Factor (VEGF), with a concentration of 50ng/ml, on the migration of HUVECs was verified. The spatial arrangement of the cells and their morphology was visualized using fluorescence and confocal microscopy. The research conducted so far is the basis for developing more complex vascularized multi-organ-on-a-chip microsystems.

Author Contributions

Conceptualization, J.K., A.Ż., and E.J.; methodology, J.K., A.Ż., and E.J.; validation, J.K., A.Ż., and E.J.; formal analysis, J.K.; investigation, J.K. and P.S.; data curation, J.K.; writing—original draft preparation, J.K.; writing—review and editing, J.K., A.Ż., and E.J. visualization, J.K.; supervision, A.Ż., and E.J.; project administration, A.Ż., and E.J.; funding acquisition, A.Ż. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Science Center OPUS No. 2021/41/B/ST4/01725 and BIOTECHMED-3 (Warsaw University of Technology) No. 504/04496/1020/45.010420.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Aspriţoiu, V.M.; Stoica, I.; Bleotu, C.; Diaconu, C.C. Epigenetic Regulation of Angiogenesis in Development and Tumors Progression: Potential Implications for Cancer Treatment. Front. Cell Dev. Biol. 2021, 9, 689962. [Google Scholar] [CrossRef] [PubMed]
  2. Moses, S.R.; Adorno, J.J.; Palmer, A.F.; Song, J.W. Vessel-on-a-chip models for studying microvascular physiology, transport, and function in vitro. Am. J. Physiol. Cell Physiol. 2021, 320, C92–C105. [Google Scholar] [CrossRef] [PubMed]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Konopka, J.; Staniszewska, P.; Żuchowska, A.; Jastrzębska, E. A Microsystem for the Study of Vascularization. Eng. Proc. 2022, 21, 50. https://doi.org/10.3390/engproc2022021050

AMA Style

Konopka J, Staniszewska P, Żuchowska A, Jastrzębska E. A Microsystem for the Study of Vascularization. Engineering Proceedings. 2022; 21(1):50. https://doi.org/10.3390/engproc2022021050

Chicago/Turabian Style

Konopka, Joanna, Patrycja Staniszewska, Agnieszka Żuchowska, and Elżbieta Jastrzębska. 2022. "A Microsystem for the Study of Vascularization" Engineering Proceedings 21, no. 1: 50. https://doi.org/10.3390/engproc2022021050

APA Style

Konopka, J., Staniszewska, P., Żuchowska, A., & Jastrzębska, E. (2022). A Microsystem for the Study of Vascularization. Engineering Proceedings, 21(1), 50. https://doi.org/10.3390/engproc2022021050

Article Metrics

Back to TopTop