Abstract
Hedera helix L. is known for its therapeutic properties, such as analgesic, anti-inflammatory, expectorant activity. It is currently known that the characteristic therapeutic effects of ivy extracts are induced by phytocompunds, such as: saponins (hederagenin, α and β-hederin, hederacoside B and C), phytosterols (sitosterol, stigmasterol, campesterol), flavonoids, falcarinol, falcarinone, scopoline, chlorogenic acid, caffeic acid, phytoestrogens [1]. The purpose of our study was to evaluate the total saponin content of Hedera helix L. leaves extracts obtained by both conventional, and unconventional methods. The commercial fresh leaves of Hedera helix L. were purchased from Hofigal SA, Romania. The following reagents used for testing were α-hederin, hederagenin, and hederacoside C at purity ≥98% (HPLC), DMSO were purchased from Sigma Aldrich. The chemical composition of the obtained extracts was analyzed by HPLC-MS/MS, and the total saponin content was evaluated [2,3]. Our study indicated an optimal method for obtaining Hedera helix L. leaves extract with an enriched saponin content.
Author Contributions
Conceptualization, C.Z. and I.C.; methodology, C.Z. and R.T.; validation, L.M., I.C. and C.Z.; resources, A.D.R. provided the plant material and made the qualitative phytochemical screening of the leaves; C.Z. obtained the extract by conventional methods; A.G. and I.C. obtained the extract by unconventional methods; R.T. determined the total saponin content; I.T. analyzed the samples tested by HPLC-MS/MS targeting quantification and interpreted the data; writing—original draft preparation, C.Z. and R.T.; writing supervision, L.M. and I.C.; funding acquisition, C.Z., R.T. and L.M. All authors have read and agreed to the published version of the manuscript.
Funding
This research was financed by the Project 381PED/2020.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data presented in this study are available on request from the corresponding author. The data are not public available, because these will be published in an article.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Lutsenko, Y.; Bylka, W.; Matławska, I.; Darmohray, R. Hedera helix as a medicinal plant. Herba Pol. 2010, 56, 83–95. [Google Scholar]
- Hiai, S.; Oura, H.; Hakajima, T. Color reaction of some sapogenins and saponins with vanillin and sulfuric acid. Planta Med. 1976, 29, 116–122. [Google Scholar] [CrossRef] [PubMed]
- Wang, Y.; Yang, L.; Zhihui, L.; Jun, Y. The quality control of the effective fraction from Dioscoreaspongiosa. Asian J. Trad. Med. 2007, 2, 12–18. [Google Scholar]
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