Next Article in Journal
Biocatalytic Preparation of Perillic Derivatives as an Alternative to Limonene Valorization
Previous Article in Journal
Nanostructured Carbon Adsorbents for Water Depollution
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Chemistry Proceedings
Volume 7
Issue 1
10.3390/chemproc2022007053
chemproc-logo
Submit to this Journal
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

Optimized Extraction of Glycoproteins from Ganoderma lucidum †

by
Naomi Tritean
1,2,
Ștefan-Ovidiu Dima
1,
Anisoara Cimpean
2,
Diana Constantinescu-Aruxandei
1,* and
Florin Oancea
1,*
1
National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, 202 Spl. Independentei, 060021 Bucharest, Romania
2
Faculty of Biology, University of Bucharest, 91-95 Spl. Independentei, 050095 Bucharest, Romania
*
Authors to whom correspondence should be addressed.
†
Presented at the 17th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM, Bucharest, Romania, 27–29 October 2021.
Chem. Proc. 2022, 7(1), 53; https://doi.org/10.3390/chemproc2022007053
Published: 23 March 2022
(This article belongs to the Proceedings of The 17th International Symposium “Priorities of Chemistry for a Sustainable Development” PRIOCHEM)
Versions Notes

Ganoderma mushrooms are a valuable source of bioactive compounds [1]. One type are glycoproteins, which were demonstrated to be able to support symbiotic bacterial biofilm formation and aid in the dispersal of dysbiotic biofilm [2]. The aim of this study was to optimize the extraction of glycoproteins from Ganoderma lucidum in order to use them in various applications, such as obtaining oral health products. A three factors, two levels optimization plan was used for glycoprotein extraction from Ganoderma lucidum powder. The chosen plan aims to evaluate the statistical significance of the following interaction terms: three main effects (A, B, C), three secondary interaction effects (A × B, B × C, A × C) and a tertiary effect (A × B × C). Following ANOVA analysis, each effect is associated with a p value, which is a measure of the correlation between effect and response variabilities. Total protein was determined using a copper-based assay (Biuret) against a bovine serum albumin (BSA) standard curve [3]. The molecular weights of the proteins were analysed on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total water-soluble carbohydrates were measured using a phenol–sulfuric acid (PSA) assay against a glucose standard curve. The extracts were characterized using Fourier transform infrared spectroscopy (FTIR). The analysis of the experimental data pointed out to a correlation between the input and output variables, exhibiting a suitable ratio between the interaction terms, in order to increase the glycoprotein extraction yield. The SDS-PAGE profile shows a narrow distribution of molecular weights (MW), with several intense bands under 5 kDa. FTIR analysis showed structural bond vibrations caused by IR radiant energy absorption that are characteristic for monosaccharide, amidic and glycosidic bonds at specific frequencies/wavenumbers, and showed a variation in the intensity and position of bands within the experimental parameters. The data analysis provided an optimized process in order to obtain glycoproteins from Ganoderma lucidum, which could be used for different biomedical applications.

Author Contributions

Conceptualization, F.O., D.C.-A.; methodology, N.T., Ș.O.-D, D.C.-A.; investigation, N.T., A.C., D.C.-A.; project administration, F.O.; supervision, F.O., D.C.-A.; validation N.T. A.C.; visualization, N.T.; writing—original draft preparation, N.T. writing—review and editing, D.C.-A. and F.O.; funding acquisition, D.C.-A. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by a grant of the Ministry of Research, Innovation and Digitization, CNCS/CCCDI—UEFISCDI, project number 366PED/2020, within PNCDI III and Nucleus Program, project number P.N.19.23.01.01 Smart-Bi, funded by Ministry of Research, Innovation and Digitalization.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We thank Bogdan Trică for helpful discussions in designing the optimization plan.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Sargowo, D.; Ovianti, N.; Susilowati, E.; Ubaidillah, N.; Nugraha, A.W.; Proboretno, K.S.; Failasufi, M.; Ramadhan, F.; Wulandari, H.; Waranugraha, Y.; et al. The role of polysaccharide peptide of Ganoderma lucidum as a potent antioxidant against atherosclerosis in high risk and stable angina patients. Indian Heart J. 2018, 70, 608–614. [Google Scholar] [CrossRef] [PubMed]
  2. Lynge Pedersen, A.M.; Belstrøm, D. The role of natural salivary defences in maintaining a healthy oral microbiota. J. Dent. 2019, 80, 3–12. [Google Scholar] [CrossRef] [PubMed]
  3. Chutipongtanate, S.; Watcharatanyatip, K.; Homvises, T.; Jaturongkakul, K.; Thongboonkerd, V. Systematic comparisons of various spectrophotometric and colorimetric methods to measure concentrations of protein, peptide and amino acid: Detectable limits, linear dynamic ranges, interferences, practicality and unit costs. Talanta 2012, 98, 123–129. [Google Scholar] [CrossRef] [PubMed]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Tritean, N.; Dima, Ș.-O.; Cimpean, A.; Constantinescu-Aruxandei, D.; Oancea, F. Optimized Extraction of Glycoproteins from Ganoderma lucidum. Chem. Proc. 2022, 7, 53. https://doi.org/10.3390/chemproc2022007053

AMA Style

Tritean N, Dima Ș-O, Cimpean A, Constantinescu-Aruxandei D, Oancea F. Optimized Extraction of Glycoproteins from Ganoderma lucidum. Chemistry Proceedings. 2022; 7(1):53. https://doi.org/10.3390/chemproc2022007053

Chicago/Turabian Style

Tritean, Naomi, Ștefan-Ovidiu Dima, Anisoara Cimpean, Diana Constantinescu-Aruxandei, and Florin Oancea. 2022. "Optimized Extraction of Glycoproteins from Ganoderma lucidum" Chemistry Proceedings 7, no. 1: 53. https://doi.org/10.3390/chemproc2022007053

APA Style

Tritean, N., Dima, Ș. -O., Cimpean, A., Constantinescu-Aruxandei, D., & Oancea, F. (2022). Optimized Extraction of Glycoproteins from Ganoderma lucidum. Chemistry Proceedings, 7(1), 53. https://doi.org/10.3390/chemproc2022007053

Article Metrics

Back to TopTop