Effects of Ionic Liquids on Laccase from Trametes versicolor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Laccase Purification
2.3. Enzymatic Assay
2.4. Fluorescence Spectroscopy
2.5. Molecular Dynamics Simulations
3. Results
3.1. Protein Sequence, Structure and Activity
3.2. Enzymatic Activity
3.2.1. Imidazolium Ionic Liquids
3.2.2. Amino Acid Ionic Liquids
3.3. Laccase Intrinsic Fluorescence
3.4. Molecular Dynamics Simulations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patel, A.Y.; Clark, A.K.; Paradis, N.J.; Amin, M.; Vaden, T.D.; Wu, C.; Caputo, G.A. Effects of Ionic Liquids on Laccase from Trametes versicolor. Biophysica 2021, 1, 429-444. https://doi.org/10.3390/biophysica1040031
Patel AY, Clark AK, Paradis NJ, Amin M, Vaden TD, Wu C, Caputo GA. Effects of Ionic Liquids on Laccase from Trametes versicolor. Biophysica. 2021; 1(4):429-444. https://doi.org/10.3390/biophysica1040031
Chicago/Turabian StylePatel, Aashka Y., Austin K. Clark, Nicholas J. Paradis, Meeraj Amin, Timothy D. Vaden, Chun Wu, and Gregory A. Caputo. 2021. "Effects of Ionic Liquids on Laccase from Trametes versicolor" Biophysica 1, no. 4: 429-444. https://doi.org/10.3390/biophysica1040031