Next Article in Journal
Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature
Previous Article in Journal
Perioperative Complications after Parotidectomy Using a Standardized Grading Scale Classification System
Article

Ionizing Radiation Mediates Dose Dependent Effects Affecting the Healing Kinetics of Wounds Created on Acute and Late Irradiated Skin

1
Centre de Recherche en Organogénèse Expérimentale de l’Université Laval (LOEX), Québec, QC G1J 1Z4, Canada
2
Department of Surgery, Faculty of Medicine, Université Laval, Québec, QC G1V 0A6, Canada
3
Department of Radiation Oncology, Cégep de Sainte-Foy, Québec, QC G1V 1T3, Canada
4
Department of Radiation Oncology, Centre Hospitalier Universitaire de Québec–Université Laval, Québec, QC G1R 2J6, Canada
5
Department of Mechanical Engineering, Faculty of Science and Engineering, Université Laval, Québec, QC G1V 0A6, Canada
6
Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1E 6W2, Canada
7
Department of Physics, Université Laval, Québec, QC G1V 0A6, Canada
8
Centre de Recherche sur le Cancer de l’Université Laval, Québec, QC G1R 2J6, Canada
*
Author to whom correspondence should be addressed.
Surgeries 2021, 2(1), 35-57; https://doi.org/10.3390/surgeries2010004
Received: 15 December 2020 / Revised: 21 January 2021 / Accepted: 22 January 2021 / Published: 28 January 2021
Radiotherapy for cancer treatment is often associated with skin damage that can lead to incapacitating hard-to-heal wounds. No permanent curative treatment has been identified for radiodermatitis. This study provides a detailed characterization of the dose-dependent impact of ionizing radiation on skin cells (45, 60, or 80 grays). We evaluated both early and late effects on murine dorsal skin with a focus on the healing process after two types of surgical challenge. The irradiated skin showed moderate to severe damage increasing with the dose. Four weeks after irradiation, the epidermis featured increased proliferation status while the dermis was hypovascular with abundant α-SMA intracellular expression. Excisional wounds created on these tissues exhibited delayed global wound closure. To assess potential long-lasting side effects of irradiation, radiodermatitis features were followed until macroscopic healing was notable (over 8 to 22 weeks depending on the dose), at which time incisional wounds were made. Severity scores and biomechanical analyses of the scar tissues revealed that seemingly healed irradiated skin still displayed altered functionality. Our detailed investigation of both the acute and chronic repercussions of radiotherapy on skin healing provides a relevant new in vivo model that will instruct future studies evaluating the efficacy of new treatments for radiodermatitis. View Full-Text
Keywords: radiation; ulcers; skin; wounds; wound healing; radiodermatitis; fibrosis; vascularization; mechanical properties; murine model radiation; ulcers; skin; wounds; wound healing; radiodermatitis; fibrosis; vascularization; mechanical properties; murine model
Show Figures

Figure 1

MDPI and ACS Style

Diaz, C.; Hayward, C.J.; Safoine, M.; Paquette, C.; Langevin, J.; Galarneau, J.; Théberge, V.; Ruel, J.; Archambault, L.; Fradette, J. Ionizing Radiation Mediates Dose Dependent Effects Affecting the Healing Kinetics of Wounds Created on Acute and Late Irradiated Skin. Surgeries 2021, 2, 35-57. https://doi.org/10.3390/surgeries2010004

AMA Style

Diaz C, Hayward CJ, Safoine M, Paquette C, Langevin J, Galarneau J, Théberge V, Ruel J, Archambault L, Fradette J. Ionizing Radiation Mediates Dose Dependent Effects Affecting the Healing Kinetics of Wounds Created on Acute and Late Irradiated Skin. Surgeries. 2021; 2(1):35-57. https://doi.org/10.3390/surgeries2010004

Chicago/Turabian Style

Diaz, Candice, Cindy J. Hayward, Meryem Safoine, Caroline Paquette, Josée Langevin, Josée Galarneau, Valérie Théberge, Jean Ruel, Louis Archambault, and Julie Fradette. 2021. "Ionizing Radiation Mediates Dose Dependent Effects Affecting the Healing Kinetics of Wounds Created on Acute and Late Irradiated Skin" Surgeries 2, no. 1: 35-57. https://doi.org/10.3390/surgeries2010004

Find Other Styles

Article Access Map by Country/Region

1
Back to TopTop