Silver Ions Incorporation into Nanofibers for Enhanced hMSC Viability
, Elizaveta S. Permyakova 1,2, Alexander N. Sheveyko 2, Dmitry V. Shtansky 2 and Anton M. Manakhov 1,*
Round 1
Reviewer 1 Report
- Title, silver ions implantation-please replace this word with either fabrication or application. The term implantation mostly refers to the application of biomaterials inside the body.
- Line 14, change, stimulate of regeneration to stimulate regeneration.
- Line 50, add to before H2O2-induced
- Were nanofibers used as antiviral? Did the authors investigate this mechanism, or is it reported already in the literature? Please include citations or proof of your findings; otherwise, eliminate these lines from the text.
Author Response
We thank the Reviewer for his help and suggestions. We carefully revised the manuscript and the answers to all questions are below. The revised manuscript after proofreading with all track changes is also attached.
- Title, silver ions implantation-please replace this word with either fabrication or application. The term implantation mostly refers to the application of biomaterials inside the body.
Answer:
The title was modified accordingly
2) Line 14, change, stimulate of regeneration to stimulate regeneration.
Answer:
The sentence was corrected.
3) Line 50, add to before H2O2-induced
Answer:
Corrected
4) Were nanofibers used as antiviral? Did the authors investigate this mechanism, or is it reported already in the literature? Please include citations or proof of your findings; otherwise, eliminate these lines from the text.
ANSWER:
Yes, the nanofibers were tested before for their antiviral efficiency (Ref15), however, that was not the main task for this work. So we removed the terms from the materials and methods section.
Reviewer 2 Report
The article entitled “Silver ions implantation onto nanofibrous surfaces for en-2 hanced hMSC viability” describes the fabrication of no of series polycaprolactone-based nanomaterials were fabricated by electrospinning and modified by deposition of TiO2 layer and implantation of silver ions (up to 0.6 at.%). Due to the implantation of the silver ions on nanofibers resulted in enhanced cell viability and proliferation. This would help in curing chronic wounds and injuries with large area of damage by activating host cells proliferation.
1. However, there are certain areas in this manuscript in which improvement is must needed. The author should explain or reason for the destruction of nanofibrous material at 15 kV Ag+ implantation (Figure 1b) as compared to PCL/PEO-ref.
2. The full form for the abbreviations like XPS analysis and the WCA describe in table 1 should be provided.
3. Please include the reference “Silver-nanoparticle-Incorporated composite nanofibers for potential wound-dressing applications” Journal of Applied Polymer Science 132(35)
Decision: Accept after the above minor revision.
Author Response
We are grateful to the Reviewer for his/her comments and suggestions and we report the answers below. All changes are implemented into the manuscript
- The author should explain or reason for the destruction of nanofibrous material at 15 kV Ag+ implantation (Figure 1b) as compared to PCL/PEO-ref.
Answer:
We added the following explanation to the text:
“The damage of PCL nanofibrous structure for the high current and high implanting voltage conditions was caused by the highly energetic ions as well as “nanodroplets” of the silver nanomaterials approaching the surface. At higher current and higher voltage stronger thermal heating of the substrate (PCL nanofibers) led to significant damage of the nanostructure.”
- The full form for the abbreviations like XPS analysis and the WCA describe in table 1 should be provided.
Answer:
The XPS and WCA abbreviations are given in section 2.3:
“The sample chemical characterization was performed by X-ray photoelectron spectroscopy (XPS)”
“The sample wettability was assessed by measuring the water contact angle (WCA).”
- Please include the reference “Silver-nanoparticle-Incorporated composite nanofibers for potential wound-dressing applications” Journal of Applied Polymer Science 132(35)
Answer:
The reference is introduced in our manuscript (Ref5)
