Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Protocol
2.2. Hematoxylin-and-Eosin (H&E) Staining and Picrosirius Red Staining
2.3. SWE Measurements
2.4. MRI
2.5. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)
2.6. M2BP ELISA Measurement
2.7. Statistical Analysis
3. Results
3.1. Evaluation of Mouse Liver Histology
3.2. Changes in SWE Values
3.3. MRI (T1rho, T2) Maps
3.4. Relationship between SWE Values and MRI Parameters
3.5. Comparison of SWE Results with Levels of a Liver Fibrosis Biomarker and Hepatic Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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A. Comparison of factors of mice in groups 1 and 2. | ||||||||
M2BP | Tgf-β | Tnf-α | iNos | |||||
R | p | R | p | R | p | R | p | |
SWE | 0.7 | <0.05 | 0.21 | n.s. | −0.50 | 0.1 | 0.02 | n.s. |
T1rho | 0.9 | <0.001 | 0.27 | n.s. | −0.33 | n.s. | 0.60 | <0.05 |
T2 | 0.53 | 0.08 | 0.11 | n.s. | −0.23 | n.s. | 0.64 | <0.05 |
B. Comparison of factors of mice in groups 1 and 3. | ||||||||
M2BP | Tgf-β | Tnf-α | iNos | |||||
R | p | R | p | R | p | R | p | |
SWE | −0.28 | n.s. | 0.17 | n.s. | 0.23 | n.s. | −0.21 | n.s. |
T1rho | 0.6 | <0.05 | 0.77 | <0.005 | 0.83 | <0.001 | 0.66 | <0.05 |
T2 | 0.6 | <0.05 | 0.77 | <0.005 | 0.73 | <0.01 | 0.80 | <0.005 |
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Futani, Y.; Hamano, M.; Matsumoto, R.; Hashimoto, S.; Nishimura, R.; Ueda, M.; Arihara, N.; Fujii, H.; Ono, M.; Miyoshi, E.; et al. Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease. Gastrointest. Disord. 2022, 4, 153-164. https://doi.org/10.3390/gidisord4030015
Futani Y, Hamano M, Matsumoto R, Hashimoto S, Nishimura R, Ueda M, Arihara N, Fujii H, Ono M, Miyoshi E, et al. Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease. Gastrointestinal Disorders. 2022; 4(3):153-164. https://doi.org/10.3390/gidisord4030015
Chicago/Turabian StyleFutani, Yoko, Megumi Hamano, Riku Matsumoto, Saya Hashimoto, Rikuto Nishimura, Mika Ueda, Narumi Arihara, Hideki Fujii, Masafumi Ono, Eiji Miyoshi, and et al. 2022. "Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease" Gastrointestinal Disorders 4, no. 3: 153-164. https://doi.org/10.3390/gidisord4030015