Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity
Abstract
:1. Introduction
2. Material and Methods
2.1. Molecular Identification of V. parahaemolyticus and V. alginolyticus
2.2. Hydrophobicity Analysis
2.3. Detection of Capsule Production
2.4. Adherence Assay
2.5. Determination of Cell Adherence and Invasion
2.6. Cytotoxicity Assay
2.7. Statistical Analysis
3. Results
3.1. Molecular Identification of Isolates
3.2. Hydrophobicity
3.3. Capsule Production and Biofilm Formation on Polystyrene Plates
3.4. Adhesion and Invasion to Human Epithelial Cells
3.5. Cytotoxicity of the Bacterial Isolates to Hep-2 Cells
3.6. Relationship between Hydrophobicity, Biofilm, Adherence, Invasion and Cytotoxicity of the Vibrio Strains
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Strains | Capsule Production on CRA | * Adhesion to N-Octane (Hydrophobicity) | ** Adhesion to Polystyrene (Biofilm Formation) | |||
---|---|---|---|---|---|---|
Phenotypes on CRA | Capsule Production | % Change in Absorbance ± (S.E.) | * Adhesion Index | ** Score Adhesion OD570 ± (S.E) | Adhesion Index | |
V. parahaemolyticus: (Spa1) | Black | Capsule producer | 60.8 ± 2.8 | Strong | 0.57 ± 0.25 | Moderately to weakly positive |
V. parahaemolyticus: (Spa2) | Black | Capsule producer | 88.2 ± 0.0 | Strong | 1.64 ± 0.20 | Highly positive |
V.parahaemolyticus: (Spa3) | Black | Capsule producer | 87.3 ± 1.4 | Strong | 1.36 ± 0.27 | Highly positive |
V. parahaemolyticus: (Spa4) | Pinkish red | Capsule non producer | 38.2 ± 1.4 | Moderate | 0.27 ± 0.03 | Moderately to weakly positive |
V. alginolyticus: (Va01) | Black | Capsule producer | 74.5 ± 0.0 | Strong | 1.21 ± 0.27 | Highly positive |
V. alginolyticus: (Va02) | Pinkish red | Capsule non producer | 32.4 ± 1.4 | Moderate | 0.36 ± 0.20 | Moderately to weakly positive |
V. alginolyticus: (Va03) | Red | Capsule non producer | 39.2 ± 2.8 | Moderate | 0.45 ± 0.02 | Moderately to weakly positive |
Bacterial Isolates | Cytotoxicity Level of Bacterial Isolates | |||
---|---|---|---|---|
No Cytotoxicity | Low ** | Moderate ** | High ** | |
V. parahaemolyticus | - | Sp4 | Spa1 | - |
- | - | Spa2 | - | |
- | - | Spa3 | - | |
V. alginolyticus | - | Va02 | Va01 | - |
- | Va03 | - | - |
Bacterial Isolates | Virulence Properties | ||||
---|---|---|---|---|---|
Hydrophobicity Using N-Octane vs. Adhesion to Polystyrene Plates | Adhesion to Polystyrene Plates vs. Adhesion to Hep-2 Cells | Adhesion vs. Invasion (to Hep-2 Cells) | Adhesion vs. Cytotoxicity (to Hep-2 Cells) | Invasion vs. Cytotoxicity (to Hep-2 Cells) | |
Vibrio species | +0.89 * | +0.92 * | +0.70 * | +0.80 * | +0.86 * |
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Lamari, F.; Khouadja, S.; Rtimi, S. Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity. Surfaces 2018, 1, 187-201. https://doi.org/10.3390/surfaces1010014
Lamari F, Khouadja S, Rtimi S. Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity. Surfaces. 2018; 1(1):187-201. https://doi.org/10.3390/surfaces1010014
Chicago/Turabian StyleLamari, Faouzi, Sadok Khouadja, and Sami Rtimi. 2018. "Interaction of Vibrio to Biotic and Abiotic Surfaces: Relationship between Hydrophobicity, Cell Adherence, Biofilm Production, and Cytotoxic Activity" Surfaces 1, no. 1: 187-201. https://doi.org/10.3390/surfaces1010014