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Neuroglia 2018, 1(1), 280-291;

Mediation of FoxO1 in Activated Neuroglia Deficient for Nucleoside Diphosphate Kinase B during Vascular Degeneration

Experimental Pharmacology, European Center of Angioscience, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
5th Med, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany
Y.Q. and H.H. share first authorship.
Author to whom correspondence should be addressed.
Received: 4 July 2018 / Revised: 18 August 2018 / Accepted: 3 September 2018 / Published: 7 September 2018
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The pathogenesis of diabetic retinopathy is closely associated with the breakdown of the neurovascular unit including the glial cells. Deficiency of nucleoside diphosphate kinase B (NDPK-B) results in retinal vasoregression mimicking diabetic retinopathy. Increased retinal expression of Angiopoietin-2 (Ang-2) initiates vasoregression. In this study, Müller cell activation, glial Ang-2 expression, and the underlying mechanisms were investigated in streptozotocin-induced diabetic NDPK-B deficient (KO) retinas and Müller cells isolated from the NDPK-B KO retinas. Müller cells were activated and Ang-2 expression was predominantly increased in Müller cells in normoglycemic NDPK-B KO retinas, similar to diabetic wild type (WT) retinas. Diabetes induction in the NDPK-B KO mice did not further increase its activation. Additionally, cultured NDPK-B KO Müller cells were more activated and showed higher Ang-2 expression than WT cells. Müller cell activation and Ang-2 elevation were observed upon high glucose treatment in WT, but not in NDPK-B KO cells. Moreover, increased levels of the transcription factor forkhead box protein O1 (FoxO1) were detected in non-diabetic NDPK-B KO Müller cells. The siRNA-mediated knockdown of FoxO1 in NDPK-B deficient cells interfered with Ang-2 upregulation. These data suggest that FoxO1 mediates Ang-2 upregulation induced by NDPK-B deficiency in the Müller cells and thus contributes to the onset of retinal vascular degeneration. View Full-Text
Keywords: angiopoietin-2; FoxO1; NDPK-B; neuroglia angiopoietin-2; FoxO1; NDPK-B; neuroglia

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Qiu, Y.; Huang, H.; Chatterjee, A.; Teuma, L.D.; Baumann, F.S.; Hammes, H.-P.; Wieland, T.; Feng, Y. Mediation of FoxO1 in Activated Neuroglia Deficient for Nucleoside Diphosphate Kinase B during Vascular Degeneration. Neuroglia 2018, 1, 280-291.

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