Mediation of FoxO1 in Activated Neuroglia Deficient for Nucleoside Diphosphate Kinase B during Vascular Degeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Immunofluorescence and Quantification
2.3. Cell Culture and High Glucose Stimulation
2.4. siRNA Mediated FoxO1 Knockdown in Müller Cells
2.5. Western Blot
2.6. Quantitative Real Time PCR
2.7. Statistical Analysis
3. Results
3.1. Müller Cells Are Activated in NDPK-B Deficient Retinas
3.2. Ang-2 Is Preferentially Upregulated in Müller Cells in the Retina during Vascular Degeneration
3.3. NDPK-B Deficiency Caused Enhancement of Ang-2 in Isolated Müller Cells
3.4. FoxO1 Is Required for NDPK-B Deficiency Induced Ang-2 Upregulation in Müller Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Qiu, Y.; Huang, H.; Chatterjee, A.; Teuma, L.D.; Baumann, F.S.; Hammes, H.-P.; Wieland, T.; Feng, Y. Mediation of FoxO1 in Activated Neuroglia Deficient for Nucleoside Diphosphate Kinase B during Vascular Degeneration. Neuroglia 2018, 1, 280-291. https://doi.org/10.3390/neuroglia1010019
Qiu Y, Huang H, Chatterjee A, Teuma LD, Baumann FS, Hammes H-P, Wieland T, Feng Y. Mediation of FoxO1 in Activated Neuroglia Deficient for Nucleoside Diphosphate Kinase B during Vascular Degeneration. Neuroglia. 2018; 1(1):280-291. https://doi.org/10.3390/neuroglia1010019
Chicago/Turabian StyleQiu, Yi, Hongpeng Huang, Anupriya Chatterjee, Loïc Dongmo Teuma, Fabienne Suzanne Baumann, Hans-Peter Hammes, Thomas Wieland, and Yuxi Feng. 2018. "Mediation of FoxO1 in Activated Neuroglia Deficient for Nucleoside Diphosphate Kinase B during Vascular Degeneration" Neuroglia 1, no. 1: 280-291. https://doi.org/10.3390/neuroglia1010019