Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Array Assembling: Peptide Array, Antibodies and Incubation of Peptide Arrays
2.2. Array Fluorescence Scanning
2.3. Vertical Scanning Interferometry (VSI)
2.4. Atomic Force Microscopy (AFM)
2.5. Comparison of AFM and VSI Measurements Force Microscopy (AFM)
2.6. Determining Antibody Profiles for VSI Measurements
3. Results and Discussion
3.1. Fast Scanning Large Field of View Using VSI
3.2. Qualitative Comparison Between VSI and Fluorescence-Based Detection
3.3. Spot Topology is Consistent for Measurements with AFM and VSI
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Volkmer, R.; Tapia, V.; Landgraf, C. Synthetic peptide arrays for investigating protein interaction domains. Febs. Lett. 2012, 586, 2780–2786. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Legutki, J.B.; Zhao, Z.G.; Greving, M.; Woodbury, N.; Johnston, S.A.; Stafford, P. Scalable high-density peptide arrays for comprehensive health monitoring. Nat. Commun. 2014, 5, 4785. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Katz, C.; Levy-Beladev, L.; Rotem-Bamberger, S.; Rito, T.; Rudiger, S.G.D.; Friedler, A. Studying protein-protein interactions using peptide arrays. Chem. Soc. Rev. 2011, 40, 2131–2145. [Google Scholar] [CrossRef] [PubMed]
- Christopher, L.; Zhiyuan, H.; Leroy, H.; Charles, T.C. SPR imaging for high throughput, label-free interaction analysis. Comb. Chem. High Throughput Screen 2009, 12, 741–751. [Google Scholar]
- Homola, J.; Koudela, I.; Yee, S.S. Surface plasmon resonance sensors based on diffraction gratings and prism couplers: Sensitivity comparison. Sensor Actuat. B Chem. 1999, 54, 16–24. [Google Scholar] [CrossRef]
- Bog, U.; Huska, K.; Maerkle, F.; Nesterov-Mueller, A.; Lemmer, U.; Mappes, T. Design of plasmonic grating structures towards optimum signal discrimination for biosensing applications. Opt. Express 2012, 20, 11357–11369. [Google Scholar] [CrossRef]
- Fechner, P.; Proll, F.; Albrecht, C.; Gauglitz, G. Kinetic analysis of the estrogen receptor alpha using RIfS. Anal. Bioanal. Chem. 2011, 400, 729–735. [Google Scholar] [CrossRef]
- Bog, U.; Laue, T.; Grossmann, T.; Beck, T.; Wienhold, T.; Richter, B.; Hirtz, M.; Fuchs, H.; Kalt, H.; Mappes, T. On-chip microlasers for biomolecular detection via highly localized deposition of a multifunctional phospholipid ink. Lab Chip 2013, 13, 2701–2707. [Google Scholar] [CrossRef] [PubMed]
- Striffler, J.; Mattes, D.S.; Schillo, S.; Munster, B.; Palermo, A.; Ridder, B.; Welle, A.; Trouillet, V.; Stadler, V.; Markovic, G.; et al. Replication of Polymer-Based Peptide Microarrays by Multi-Step Transfer. Chem. Nano Mat. 2016, 2, 897–903. [Google Scholar] [CrossRef]
- Souplet, V.; Desmet, R.; Melnyk, O. Imaging of protein layers with an optical microscope for the characterization of peptide microarrays. J. Pept. Sci. 2007, 13, 451–457. [Google Scholar] [CrossRef] [PubMed]
- Harasaki, A.; Schmit, J.; Wyant, J.C. Improved vertical-scanning interferometry. Appl. Optics. 2000, 39, 2107–2115. [Google Scholar] [CrossRef]
- Arvidson, R.S.; Fischer, C.; Sawyer, D.S.; Scott, G.D.; Natelson, D.; Luttge, A. Lateral Resolution Enhancement of Vertical Scanning Interferometry by Sub- Pixel Sampling. Microsc. Microanal. 2014, 20, 90–98. [Google Scholar] [CrossRef] [PubMed]
- Chong, W.K.; Li, X.; Soh, Y.C. Harnessing spectral property of dual wavelength white LED to improve vertical scanning interferometry. Appl. Optics 2013, 52, 4652–4662. [Google Scholar] [CrossRef] [PubMed]
- Little, D.J.; Kane, D.M. Measuring nanoparticle size using optical surface profilers. Opt. Express 2013, 21, 15664–15675. [Google Scholar] [CrossRef]
- Koyuncu, I.; Brant, J.; Luttge, A.; Wiesner, M.R. A comparison of vertical scanning interferometry (VSI) and atomic force microscopy (AFM) for characterizing membrane surface topography. J. Membrane Sci. 2006, 278, 410–417. [Google Scholar] [CrossRef]
- Spencer, A.; Dobryden, I.; Almqvist, N.; Almqvist, A.; Larsson, R. The influence of AFM and VSI techniques on the accurate calculation of tribological surface roughness parameters. Tribol. Int. 2013, 57, 242–250. [Google Scholar] [CrossRef]
- Scott, C.C.; Luttge, A.; Athanasiou, K.A. Development and validation of vertical scanning interferometry as a novel method for acquiring chondrocyte geometry. J. Biomed. Mater. Res. A 2005, 72a, 83–90. [Google Scholar] [CrossRef]
- Weber, L.K.; Palermo, A.; Kugler, J.; Armant, O.; Isse, A.; Rentschler, S.; Jaenisch, T.; Hubbuch, J.; Dubel, S.; Nesterov-Mueller, A.; et al. Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays. J. Immunol. Methods 2017, 443, 45–54. [Google Scholar] [CrossRef] [PubMed]
- Wilson, I.A.; Niman, H.L.; Houghten, R.A.; Cherenson, A.R.; Connolly, M.L.; Lerner, R.A. The Structure of an Antigenic Determinant in a Protein. Cell 1984, 37, 767–778. [Google Scholar] [CrossRef]
- Pounder, R.J.; Stanford, M.J.; Brooks, P.; Richards, S.P.; Dove, A.P. Metal free thiol-maleimide ‘Click’ reaction as a mild functionalization strategy for degradable polymers. Chem. Commun. 2008, 41, 5158–5160. [Google Scholar] [CrossRef]
- Olszak, A.; Zecchino, M. Lateral-scanning interferometry takes the long view. Photon Spectra 2002, 36, 54–56. [Google Scholar]
- Necas, D.; Klapetek, P. Gwyddion: An open-source software for SPM data analysis. Cent. Eur. J. Phys. 2012, 10, 181–188. [Google Scholar]
- Tan, Y.H.; Liu, M.; Nolting, B.; Go, J.G.; Gervay-Hague, J.; Liu, G.Y. A Nanoengineering Approach for Investigation and Regulation of Protein Immobilization. Acs Nano 2008, 2, 2374–2384. [Google Scholar] [CrossRef] [Green Version]
- Tatikolov, A.S.; Costa, S.M.B. Energy transfer and fluorescence quenching in complexes of polymethine dyes with human serum albumin. Photochem Photobiol 2004, 80, 250–256. [Google Scholar] [CrossRef] [PubMed]
- Reineck, P.; Gomez, D.; Ng, S.H.; Karg, M.; Bell, T.; Mulvaney, P.; Bach, U. Distance and Wavelength Dependent Quenching of Molecular Fluorescence by Au@SiO2 Core-Shell Nanoparticles. Acs Nano 2013, 7, 6636–6648. [Google Scholar] [CrossRef] [PubMed]
- Eisenthal, R.; Danson, M.J. (Eds.) Enzyme Assays: A practical Approach; University Press: Oxford, UK, 2002; ISBN 978-0-19-963820-8. [Google Scholar]
- Deegan, R.D.; Bakajin, O.; Dupont, T.F.; Huber, G.; Nagel, S.R.; Witten, T.A. Capillary flow as the cause of ring stains from dried liquid drops. Nature 1997, 389, 827–829. [Google Scholar] [CrossRef]
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Palermo, A.; Thelen, R.; Weber, L.K.; Foertsch, T.; Rentschler, S.; Hackert, V.; Syurik, J.; Nesterov-Mueller, A. Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions. High-Throughput 2019, 8, 7. https://doi.org/10.3390/ht8020007
Palermo A, Thelen R, Weber LK, Foertsch T, Rentschler S, Hackert V, Syurik J, Nesterov-Mueller A. Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions. High-Throughput. 2019; 8(2):7. https://doi.org/10.3390/ht8020007
Chicago/Turabian StylePalermo, Andrea, Richard Thelen, Laura K. Weber, Tobias Foertsch, Simone Rentschler, Verena Hackert, Julia Syurik, and Alexander Nesterov-Mueller. 2019. "Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions" High-Throughput 8, no. 2: 7. https://doi.org/10.3390/ht8020007
APA StylePalermo, A., Thelen, R., Weber, L. K., Foertsch, T., Rentschler, S., Hackert, V., Syurik, J., & Nesterov-Mueller, A. (2019). Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions. High-Throughput, 8(2), 7. https://doi.org/10.3390/ht8020007