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High-Throughput 2019, 8(1), 6; https://doi.org/10.3390/ht8010006

Protein Adductomics: Methodologies for Untargeted Screening of Adducts to Serum Albumin and Hemoglobin in Human Blood Samples

1
Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, CA 94720, USA
2
Department of Environmental Science and Analytical Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Received: 29 January 2019 / Revised: 26 February 2019 / Accepted: 27 February 2019 / Published: 8 March 2019
(This article belongs to the Special Issue Adductomics: Elucidating the Environmental Causes of Disease)
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Abstract

The reaction products of electrophiles in vivo can be measured as adducts to the abundant proteins, hemoglobin (Hb), and human serum albumin (HSA), in human blood samples. During the last decade, methods for untargeted screening of such adducts, called “adductomics”, have used liquid chromatography-mass spectrometry to detect large numbers of previously unknown Hb and HSA adducts. This review presents methodologies that were developed and used in our laboratories for Hb and HSA adductomics, respectively. We discuss critical aspects regarding choice of target protein, sample preparation, mass spectrometry, data evaluation, and strategies for identification of detected unknown adducts. With this review we give an overview of these two methodologies used for protein adductomics and the precursor electrophiles that have been elucidated from the adducts. View Full-Text
Keywords: proteins; protein adducts; electrophiles; adductomics; mass spectrometry; hemoglobin; human serum albumin proteins; protein adducts; electrophiles; adductomics; mass spectrometry; hemoglobin; human serum albumin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Carlsson, H.; Rappaport, S.M.; Törnqvist, M. Protein Adductomics: Methodologies for Untargeted Screening of Adducts to Serum Albumin and Hemoglobin in Human Blood Samples. High-Throughput 2019, 8, 6.

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