Hydrolase-Like Activity Provided by Zinc(II) and Oleoyl-Histidine at Liposome Membrane Surface
Abstract
:1. Introduction
2. Results and Discussion
2.1. Evaluation of Hydrolysis Catalytic Activity
2.2. Evaluation of Complex Creation
2.3. Evaluation of the Membrane Property of O-His Modified Liposomes in the Presence of Zn2+
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Synthesis of O-His
4.3. Liposome Preparation
4.4. Evaluation of Hydrolysis Activity
4.5. Evaluation of Zn-Imidazole Complex by Raman Spectroscopy
4.6. Evaluation of Membrane Fluidity and Polarity
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
CA | carbonic anhydrase |
O-His | oleoyl-histidine |
p-NPA | p-nitrophenylacetate |
DOPC | 1,2-dioleoyl-sn-glycero-3-phosphocholine |
DPPC | 1,2-dipalmitoyl-sn-glycero-3-phosphocholine |
Dansyl-DHPE | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl) |
Laurdan | 6-lauroyl-2-dimethylamino-naphthalene |
DPH | 1,6-diphenyl-1,3,5-hexatriene (DPH), |
TMA-DPH | 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene |
p-NP | p-nitrophenol |
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Tauchi, A.; Suga, K.; Umakoshi, H. Hydrolase-Like Activity Provided by Zinc(II) and Oleoyl-Histidine at Liposome Membrane Surface. Colloids Interfaces 2018, 2, 24. https://doi.org/10.3390/colloids2020024
Tauchi A, Suga K, Umakoshi H. Hydrolase-Like Activity Provided by Zinc(II) and Oleoyl-Histidine at Liposome Membrane Surface. Colloids and Interfaces. 2018; 2(2):24. https://doi.org/10.3390/colloids2020024
Chicago/Turabian StyleTauchi, Atsushi, Keishi Suga, and Hiroshi Umakoshi. 2018. "Hydrolase-Like Activity Provided by Zinc(II) and Oleoyl-Histidine at Liposome Membrane Surface" Colloids and Interfaces 2, no. 2: 24. https://doi.org/10.3390/colloids2020024