Thermal Properties of Multilayer Nanocomposites Based on Halloysite Nanotubes and Biopolymers

Abstract

This paper reports a novel procedure to fabricate multilayer composite biofilms based on halloysite nanotubes (HNTs) and sustainable polymers. Among the biopolymers, the non-ionic (hydroxypropyl cellulose) and cationic (chitosan) molecules were selected. The nanocomposites were prepared by the sequential casting of ethanol solutions of hydroxypropyl cellulose and aqueous dispersions of chitosan/HNTs. The composition of the bio-nanocomposites was systematically changed in order to investigate the effect of the hydroxypropyl cellulose/HNTs ratio on the thermal properties of the films, which were investigated by differential scanning calorimetry (DSC) and thermogravimetry (TG). DSC studies were conducted in the static air (oxidative atmosphere), while TG measurements were carried out under nitrogen flow (inert atmosphere). The analysis of DSC data provided the enthalpy and the temperature for the oxidative degradation of the bio-nanocomposites. These results were helpful to estimate the efficacy of the well-compacted middle layer of HNTs as a flame retardant. TG experiments were performed at a variable heating rate and the collected data were analyzed by the Friedman’s method (non-isothermal thermogravimetric approach) with the aim of studying the kinetics of the hydroxypropyl cellulose degradation in the multilayer nanocomposites. This work represents an advanced contribution for designing novel sustainable nanocomposites with excellent thermal behavior as a consequence of their peculiar multilayer structure.

1. Introduction

Halloysite is an emerging nanofiller for the fabrication of smart nanocomposites with specific functionalities useful for several technological purposes, such as anti-corrosion coatings [1,2,3], food packaging [4,5,6], remediation [7,8,9,10,11], catalysis [12,13,14,15], tissue engineering [16,17,18], preservation of art-works [19,20,21], and antimicrobial protection [22,23]. Recent biological studies (by means of both in vivo and vitro tests) proved that halloysite exhibits a low toxicity [24,25,26]. The chemical formula of halloysite is Al₂Si₂O₅(OH)₄·2H₂O. From the mineralogical viewpoint, halloysite is a phyllosilicate 1:1, which means that each composed layer is formed of one octahedral sheet of alumina and one tetrahedral sheet of silica [27]. This mineral possesses a hollow tubular shape with a large polydispersity in sizes on the dependence of the geological setting [28]. The length interval is between 50 and 1500 nm, while the external and internal diameters range between 20–150 and 10–15 nm [28]. The chemical composition of the halloysite surfaces is different, being that the inner one is composed of SiO₂ groups while the external shell is formed by Al₂O₃ groups. Consequently, in the pH 2–8, the cavity possesses a positive net charge, whereas the shell is negatively charged [29]. This peculiarity allows for a selective modification of the halloysite surfaces by means of ionic molecules that can be exploited to control its colloidal stability and rheological properties as requested for the numerous applications on tubular nanoparticles [30,31,32,33]. Within this, the specific electrostatic interactions between ionic polymers and halloysite affect the thermal and mechanical behaviors of the corresponding nanocomposites prepared by the aqueous casting method [34]. The addition of halloysite nanotubes (HNTs) within anionic polymers represents an efficient strategy to enhance the thermal stability of the biomaterials because the polymeric chains are entrapped within the HNTs lumen [4,34]. This effect was detected for pectin/HNTs [4,35] and alginate/HNTs nanocomposites [34]. Biocomposites based on cationic chitosan and halloysite evidenced suitable performances as scaffolds for tissue engineering [18,23,36] and hydrogel for medical purposes [37]. The literature reports that HNTs are efficient reinforcing fillers for chitosan matrix [38,39]. The high dispersibility of halloysite into hydrophilic polymers generated nanocomposite materials with a large wettability and excellent tensile performances in terms of stress at breaking point and elasticity [34]. Nanocomposites based on halloysite and polylactic acid (PLA) showed enhanced barrier properties with respect to the pure biopolymer [40]. Our recent work evidenced that the confinement of HNTs between chitosan layers generated composite biofilms with a relevant flame resistance [41]. It should be noted that the specific functionalities and the physicochemical characteristics of polymer/HNTs nanocomposites are strongly influenced by their mesoscopic structure [42]. Here, we prepared multilayer nanocomposite films formed by three components: two biopolymers (hydroxypropyl cellulose and chitosan) and halloysite (inorganic filler). An intermediate layer of chitosan/HNT was confined between hydroxypropyl cellulose by using a sequential casting procedure performed in different solvents (water and ethanol). The thermal properties of the nanocomposites were investigated by two techniques: thermogravimetry (TG) and differential scanning calorimetry (DSC). The combination of TG and DSC can provide an exhaustive description of the thermal behavior of hybrid systems, including supramolecular complexes [43,44,45] and polymer/nanofiller composite materials [41,46,47]. Moreover, the mentioned thermal methods are useful within a cultural heritage for the diagnostics [48] and the treatment [20] of artworks. As reported in this paper, TG experiments performed at variable heating rates allow for the determination of the activation energy of the polymer degradation in composite systems [5,49,50]. On the other hand, DSC measurements are helpful to investigate the flame retardant action of nanofillers dispersed in a polymeric matrix [41,51].

This paper reports a novel protocol to generate biopolymers/halloysite nanocomposites with a peculiar sandwich-like shape. The promising thermal properties in the inert atmosphere were investigated by thermogravimetric experiments using a non-isothermal approach, while differential scanning calorimetry measurements in static air revealed that the multilayer morphology confers a fire resistance to the nanocomposites. This study provides a contribution to the fabrication of bio-nanocomposites with a controlled structure that can be exploited for engineering and biological applications.

2. Materials and Methods

2.1. Materials

Chitosan (75–85% deacetylated, M_w = 50–190 kg·mol⁻¹), hydroxypropyl cellulose (HPC, M_w = 80 kg·mol⁻¹), ethanol, and glacial acetic acid are Aldrich products (Sigma-Aldrich, St. Louis, MO, USA). Halloysite nanotubes (HNTs; M_w = 294.19 g·mol⁻¹) are from Imerys Ceramics New Zeeland Limited (Kerikeri, New Zealand). The specific surface of halloysite is 28.64 m²·g⁻¹ [28].

2.2. Preparation of Multilayer Nanocomposites

The multilayer nanocomposites were prepared through a sequential casting method. Firstly, we prepared the solutions of chitosan and HPC in two different solvents (water and ethanol, respectively) and an aqueous dispersion of halloysite. The concentrations and the solvent media used for the biopolymer solution are reported in

Table 1. The concentration and solvent medium for the biopolymer solutions.

Material	Solvent	Concentration (wt %)
Hydroxypropyl cellulose (HPC)	ethanol	2.01
Chitosan	water (pH = 3.5)	1.02

.

Stable polymer solutions were obtained by stirring overnight at 30 °C. As concerns chitosan, the pH of the solution was fixed at 3.5 by adding dropwise 0.1 mol·dm⁻³ acetic acid because of the very low solubility of the biopolymer at neutral pH [29]. Then, halloysite was dispersed in the chitosan solution by sonication and subsequent magnetically stirring overnight at 30 °C. The concentration of halloysite dispersion was 5 wt %. Consequently, the chitosan/HNTs ratio was fixed at 0.2. Chitosan acted as the stabilizing agent of the HNT’s aqueous dispersions.

The nanocomposite films with the multilayer structure (a layer of chitosan/HNTs between two layers of HPC) were obtained through the subsequent deposition of the HPC solution and the chitosan/HNTs dispersion as follows: (1) HPC; (2) chitosan/HNTs; (3) HPC.

In detail, the first layer of the nanocomposite was obtained by pouring the HPC solution into a glass Petri dish at 30 °C to evaporate ethanol until the weight was constant. Then, the chitosan/HNTs aqueous dispersion was deposited on the HPC dried film and kept at 30 °C to evaporate water and, consequently, we obtained the second layer. Finally, the casting of the HPC was conducted on the chitosan/HNTs layer allowing to fabricate a sandwich-like structure. It should be noted that the very low solubility of chitosan in ethanol ensures to obtain nanocomposites with a multilayer morphology.

The composition of the nanocomposites was systematically varied by changing the amounts of HPC solutions and chitosan/HNTs used in the sequential casting procedure (

Table 2. The composition of the nanocomposites and the corresponding amounts of hydroxypropyl cellulose (HPC) solutions and chitosan/halloysite nanotubes (HNTs) dispersions used in the sequential casting.

R(C+H)/HPC	Amount of HPC Solution (First Layer)/g	Amount of Chitosan/HNTs Dispersion (Second Layer)/g	Amount of HPC Solution (Third Layer)/g
0.92	8.18	4.96	8.02
1.89	8.15	10.24	8.07
2.81	8.05	15.03	8.00

). The variable composition of the nanocomposites was expressed by the weight ratio (R_(C+H)/HPC) between the middle layer (chitosan + HNTs) and outer layers (HPC).

2.3. Methods

2.3.1. Thermogravimetry

The Q5000 IR apparatus (TA Instruments, New Castle, DE, USA) was employed to perform thermogravimetry (TG) measurements, which were carried out under a nitrogen flows of 25 and 10 cm³·min⁻¹ for the sample and the balance, respectively. The sample (ca. 5 mg) was loaded in a platinum crucible and heated from room temperature to 900 °C at variable heating rates (β), which were 5, 10, 15, and 20 °C·min⁻¹. The analysis of TG data allowed for the determination of the activation energy (E_a) of HPC degradation. The Curie temperatures of standards (nickel, cobalt, and their alloys) were considered for the temperature calibration.

2.3.2. Differential Scanning Calorimetry

The DSC 2920 CE apparatus (TA Instruments) was used to conduct Differential scanning calorimetry (DSC) measurements. The apparatus was calibrated using the using the melting enthalpy of standard indium (28.71 J·g⁻¹). Both the temperature and the power calibration were estimated at variable heating rates in agreement with the recent IUPAC technical report [52]. The sample (ca. 2 mg) was loaded in an aluminum pan. The measurements were performed in the static air from 0 to 600 °C at the heating rate of 10 °C·min⁻¹.

2.3.3. Scanning Electron Microscopy

A microscope ESEM FEI QUANTA 200F (Hillsboro, OR, USA) was used to investigate the morphological characteristics of the nanocomposite, which were previously coated with gold in argon by means of an Edwards Sputter Coater S150A (Edwards, Burgess Hill, UK) to avoid charging under the electron beam. The measurements were conducted in a high vacuum (<6 × 10⁻⁴ Pa), while the energy of the beam and the working distance were set at 25 kV and 10 mm, respectively.

3. Results and Discussion

3.1. Multilayer Structure the Nanocomposites

Figure 1 shows the schematic representation and the scanning electron microscope (SEM) image for the prepared multilayer nanocomposites.

The thickness of the outer HPC layers was kept constant, while the intermediate layer of the chitosan/HNTs was systematically changed. Regarding the HPC layers, the thickness (h_(HPC)) was calculated as

h_(HPC) = m_HPC/(δ_HPC∙π∙r²),

(1)

where r is the radius of the Petri dish used for the preparation of the nanocomposites, while m_HPC and δ_HPC are the mass and the density of HPC, respectively. The thickness of the intermediate layer composed by chitosan and HNTs (h_C+H) was determined by the equations

h*_(C+H) = h_(C) + h_(H),

(2)

h_(C) = m_C/(δ_C∙π∙r²),

(3)

h_(H) = m_H/(δ_H∙π∙r²),

(4)

where m_C and δ_C are the mass and the density of chitosan, respectively, and m_H and δ_H are the mass and the density of halloysite, respectively. It should be noted that the described approach (Equations (2)–(4)) assumes that the densities of chitosan and halloysite are not altered by their reciprocal interactions. The sum of the calculated thicknesses for each composed layers is in agreement with the total thickness (experimentally determined by a micrometer) of the nanocomposite film.

Table 3. The thicknesses of the films, the outer HPC layers, and intermediate chitosan/HNT layer for the prepared nanocomposites.

R(C+H)/HPC	h(HPC) (First Layer)/μm	h(HPC) (Third Layer)/μm	hC+H (Middle Layer)/μm	Nanocomposite Thickness/μm
0.92	71.2	69.8	2.67	144
1.89	70.9	70.3	5.52	147
2.81	70.1	69.7	8.11	149

collects the thickness values for the prepared nanocomposites.

3.2. Thermal Properties of the Nanocomposites

3.2.1. Thermogravimetric Analysis: Thermal Behavior under Inert Atmosphere

Figure 2 shows the thermogravimetric curve (determined at β = 10 °C·min⁻¹) of the nanocomposite at variable compositions.

We observed three different mass losses: (1) the first mass loss (ML₁₅₀) occurs in the temperature range between 25 and 150 °C as a consequence of the evaporation of the water physically adsorbed on the materials; (2) the second mass loss (ML₄₀₀) takes place at the temperature interval 200–400 °C because of the decomposition of the organic moieties; (3) the third mass loss (ML550) occurs in a temperature range of 420–550 °C. As reported in the literature [34], the latter is due to the expulsion of the two water molecules that are present in the halloysite interlayer.

Table 4. The mass losses and residual masses at 900 °C for pure HPC and nanocomposites determined by thermogravimetry (TG) measurements at β = 10 °C·min⁻¹.

R(C+H)/HPC	ML150/%	ML400/%	ML600/%	MR900/%	TCHIT/°C	THPC/°C
0	0.65	92.4	/	4.07	289.2	369.7
0.92	1.74	61.3	4.28	30.1	290.2	376.4
1.89	1.83	43.2	6.33	44.8	291.6	373.4
2.81	2.31	42.1	6.49	45.8	286.5	374.5

compares the mass losses for the nanocomposite materials with those for pure hydroxypropyl cellulose.

The presence of the intermediate layer composed of chitosan and HNT generated an increase of the ML₁₅₀ indicating an enhancement of the affinity towards water due to the hydrophilic nature of halloysite. As expected, the ML₄₀₀ value was reduced in the nanocomposites, while the opposite effect was detected for ML₆₀₀ results. Compared with neat HPC, MR₉₀₀ is much larger in the composite materials because of the inorganic clay nanotubes, which do not undergo through a complete decomposition even at very high temperatures [34]. The analysis of the differential thermogravimetric (DTG) curves (Figure 3) provided a clearer description of the several degradation steps occurring in the nanocomposites.

In the range 200–400 °C, DTG curves showed a shoulder (centered at ca. 290 °C) and a peak (centered at ca. 370 °C) that can be mostly attributed to the thermal degradation of chitosan and HPC, respectively. Accordingly, we determined the HPC degradation temperature (T_HPC) from the maximum of the DTG peaks, while the chitosan degradation temperature (T_CHIT) was estimated from the maximum of the DTG shoulders. As evidenced in Table 4, a slight thermal stabilization of HPC was observed in the multilayer nanocomposites. Generally, an increase of the polymer resistance to thermal degradation in nanocomposite systems is ascribed to the barrier effect of the nanofillers towards the volatile products generated by the polymer decomposition [34,46]. Regarding chitosan, the degradation temperatures are similar to that of pure chitosan (290.2 °C).

The kinetic aspects of the polymer degradation were studied by using a non-isothermal thermogravimetric method. To this purpose, we performed TG measurements at variable heating rates and the obtained data in the range 200–400 °C were analyzed by means of the Friedman approach, which can be expressed by the equation

$$\ln \left(\frac{\mathsf{\beta }\mathrm{d}\mathsf{\alpha }}{\mathrm{dT}}\right)=\ln \left[\mathrm{Af}\left(\mathsf{\alpha }\right)\right]-\frac{{\mathrm{E}}_{\mathrm{a}}}{\mathrm{RT}}$$

(5)

where α is the conversion degree, while A and R represent the pre-exponential factor and the gas constant, respectively. Moreover, f(α) is the function that relates the specific degradation mechanism with the conversion degree. Based on Equation (5), the activation energy (E_a) can be determined by the slope of the ln(βdα/dT) vs. the 1/T trends. As a general result, we observed that E_a is constant with α for all of the investigated materials. On this basis, we calculated the average E_a values, which are presented in Figure 4. In general, the presence of the intermediate layer did not significantly affect the kinetics of HPC degradation.

3.2.2. Oxidative Degradation of Nanocomposites by Differential Scanning Calorimetry

The oxidative degradation of the nanocomposite materials was investigated by means of DSC measurements conducted statically. As shown in Figure 5, the DSC curves of the multilayer systems present an exothermic reaction due to the degradation of organic components under the oxidative conditions.

The analysis of the DSC peaks allowed for the exploration of the thermodynamics of the oxidative degradation. In particular, we determined the temperature (T_ox) and the enthalpy variation (∆H_ox) of the oxidative degradation by the maximum and the integration of the exothermic peaks. Figure 6 displays the dependence of both T_ox and ∆H_ox on the weight ratio between the middle layer (chitosan + HNTs) and the outer layers (HPC).

Compared with pure HPC, the nanocomposites gave evidence to the reduction of both parameters. The ∆H_ox decrease indicates that the presence of the middle layer composed of chitosan and halloysite lowered the quantity of the heat released during the exothermic process, which is represented by the oxidative degradation. This result gives evidence that the addition of chitosan/HNTs between the HPC layers produces a fire retardant action as expected for well-defined multilayer materials.

4. Conclusions

Nanocomposites with a multilayer structure were successfully prepared by the sequential casting of ethanol solutions of HPC and the aqueous dispersion of chitosan/halloysite. The composition of the nanocomposites was systematically varied in order to control the thickness of the intermediate layer composed of chitosan and halloysite nanotubes (HNTs). The thermal properties of the nanocomposites were affected by the peculiar multilayer structure of the biocomposite films. Thermogravimetry (TG) measurements performed under an inert atmosphere gave evidence to a slight increase of the HPC thermal stability in the nanocomposites. TG data collected at variable heating rates were analyzed by the non-isothermal Friedman method, which showed that the activation energy of the HPC degradation is not significantly affected by the addition of the chitosan/HNT layer.

The oxidative degradation of the nanocomposites was studied by Differential Scanning Calorimetry (DSC), which highlighted a reduction in the temperature, as well as the enthalpy variation for the investigated exothermic process in the multilayer systems. Interestingly, the decrease of the enthalpy change indicates that the chitosan/halloysite layer generates a flame retardant action on the biomaterials. In conclusion, this work describes an easy strategy to fabricate multilayer bio-nanocomposites with promising thermal characteristics.