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Spirulina’s Effect on Paraoxonase Activity †

Maja Milanović
Danijela Kojić
Nataša Milošević
Jovana Drljača Lero
Anamarija Mandić
3 and
Nataša Milić
Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia
Institute of Food Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
Author to whom correspondence should be addressed.
Presented at the 14th European Nutrition Conference FENS 2023, Belgrade, Serbia, 14–17 November 2023.
Proceedings 2023, 91(1), 186;
Published: 2 February 2024
(This article belongs to the Proceedings of The 14th European Nutrition Conference FENS 2023)


Hyperlipidaemia is a common worldwide problem associated with an increased risk of coronary and extra-coronary atherosclerosis and cardiovascular disease. Besides changes in lifestyle habits that include controlling the diet with moderate alcohol consumption and maintaining normal weight, medication is usually prescribed in addition. The antioxidative potential of functional food in the treatment of hyperlipidaemia continuously attracts growing attention. Paraoxonase enzyme (PON1) prevents the oxidation of low- and high-density lipoprotein (LDL and HDL) and, hence, has an important role in acting against lipid peroxides. The aim of this study was to evaluate Spirulina platensis’s influence on blood and hepatic PON1 activity in an animal model. Male Wistar rats (approved by the Institutional Bioethics committee No. III-2011-01) were randomly divided into five groups based on the applied diet (I—normal diet; II—normal diet with spirulina; III—lipogenic diet; IV—lipogenic diet with concomitant spirulina supplementation; and V—lipogenic diet with spirulina treatment). The differences in PON1 activity were related to diet type. A lipogenic diet rich in saturated fats impaired the PON1 activity. Both blood and hepatic PON1 activity were significantly increased after the administration of a normal diet with spirulina supplementation. As expected, significantly reduced blood PON1 activity was measured in the lipogenic diet group. Blood PON1 activity was decreased in groups III, IV, and V, but PON1 in both the blood and liver had a tendency to increase in groups IV and V. Based on the obtained results, PON1 activity is affected by hyperlipidaemia, and spirulina supplementation may promote enzyme activity.

Author Contributions

Conceptualization, A.M. and N.M. (Nataša Milić); methodology, D.K.; formal analysis, M.M. and N.M. (Nataša Milošević); investigation, M.M. and D.K.; resources, N.M. (Nataša Milić); data curation and visualization, N.M. (Nataša Milošević) and J.D.L.; writing—original draft preparation, M.M.; writing—review and editing, N.M. (Nataša Milić); supervision, N.M. (Nataša Milić); project administration, A.M.; funding acquisition, A.M. and N.M. (Nataša Milić). All authors have read and agreed to the published version of the manuscript.


This research was financially supported by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina [grant number 142-451-3509/2023-01], and the Ministry of Science, Technological Development and Innovation, Republic of Serbia [grant number 451-03-68/2022-14/200114].

Institutional Review Board Statement

The animal study protocol was approved by the Committee for the Use and Keeping of Laboratory Animals from the University of Novi Sad (protocol code III-2011-01).

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from the corresponding author.

Conflicts of Interest

The authors declare no conflict of interest.
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Share and Cite

MDPI and ACS Style

Milanović, M.; Kojić, D.; Milošević, N.; Drljača Lero, J.; Mandić, A.; Milić, N. Spirulina’s Effect on Paraoxonase Activity. Proceedings 2023, 91, 186.

AMA Style

Milanović M, Kojić D, Milošević N, Drljača Lero J, Mandić A, Milić N. Spirulina’s Effect on Paraoxonase Activity. Proceedings. 2023; 91(1):186.

Chicago/Turabian Style

Milanović, Maja, Danijela Kojić, Nataša Milošević, Jovana Drljača Lero, Anamarija Mandić, and Nataša Milić. 2023. "Spirulina’s Effect on Paraoxonase Activity" Proceedings 91, no. 1: 186.

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