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Proceeding Paper

Electrochemical Characterization of Nitrocellulose Membranes towards Bacterial Detection in Water †

1
Institute of Information and Communication Technologies, Electronics and Applied Mathematics, UCLouvain, 1348 Louvain-la-Neuve, Belgium
2
Laboratory of Food and Environmental Microbiology, Earth and Life Institute, UCLouvain, 1348 Louvain-la-Neuve, Belgium
3
Institute of Condensed Matter and Nanosciences (Bio and Soft Matter), UCLouvain, 1348 Louvain-La-Neuve, Belgium
*
Author to whom correspondence should be addressed.
Presented at the 1st International Electronic Conference on Biosensors, 2–17 November 2020; Available online: https://iecb2020.sciforum.net/.
Proceedings 2020, 60(1), 61; https://doi.org/10.3390/IECB2020-07080
Published: 2 November 2020
(This article belongs to the Proceedings of The 1st International Electronic Conference on Biosensors)
Paper substrates have shown a high potential for development of cost-effective and efficient point-of-care biosensors, essential for public healthcare and environmental diagnostics. Most paper-based biosensors rely on qualitative colorimetric detection schemes with high limits of detection. To overcome this limitation, technologies that combine paper-based substrates and electrochemical detection are being developed to allow for quantification and achieve better performances. In this work, we explore the potential of dielectric measurements towards electrical detection of whole-cell bacteria in nitrocellulose membranes, a paper-derivative. Impedance spectroscopy was considered to characterize the membranes with and without Bacillus thuringiensis cells, used as model microorganism. To specifically target this bacterial strain, phage endolysin cell-wall binding domain (CBD) encoded by a bacteriophage targeting B. thuringiensis were prepared and integrated into the membranes as recognition biointerface. The fluid sample containing the bacteria is conducted in the membrane through passive capillarity, and the bacteria are specifically immobilized in the test zone. Resulting changes of the dielectric properties of the membrane are sensed through impedance changes, highlighting the contribution of ions in the bacterial detection mechanism. This experimental proof-of-concept illustrates the electrical detection of 108 CFU/mL bacteria in low-salinity buffers within 5 min.
Keywords: whole-cell bacterial detection; nitrocellulose membrane; dielectric properties; impedance spectroscopy; parallel plates; interdigital electrodes; endolysins; Bacillus thuringiensis whole-cell bacterial detection; nitrocellulose membrane; dielectric properties; impedance spectroscopy; parallel plates; interdigital electrodes; endolysins; Bacillus thuringiensis
MDPI and ACS Style

Brun, G.L.; Hauwaert, M.; Leprince, A.; Glinel, K.; Mahillon, J.; Raskin, J.-P. Electrochemical Characterization of Nitrocellulose Membranes towards Bacterial Detection in Water. Proceedings 2020, 60, 61. https://doi.org/10.3390/IECB2020-07080

AMA Style

Brun GL, Hauwaert M, Leprince A, Glinel K, Mahillon J, Raskin J-P. Electrochemical Characterization of Nitrocellulose Membranes towards Bacterial Detection in Water. Proceedings. 2020; 60(1):61. https://doi.org/10.3390/IECB2020-07080

Chicago/Turabian Style

Brun, Grégoire Le, Margo Hauwaert, Audrey Leprince, Karine Glinel, Jacques Mahillon, and Jean-Pierre Raskin. 2020. "Electrochemical Characterization of Nitrocellulose Membranes towards Bacterial Detection in Water" Proceedings 60, no. 1: 61. https://doi.org/10.3390/IECB2020-07080

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