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Real-Time Pathogen Determination by Optical Biosensing Based on Graphene Oxide
 
 
Proceeding Paper

Quenching of Fluorescence Caused by Graphene Oxide as an Immunosensing Platform in a Microwell Plate Format †

Biophotonic Nanosensors Laboratory, Centro de Investigaciones en Óptica A.C., 37150 sLeón, Mexico
*
Author to whom correspondence should be addressed.
Presented at the 1st International Electronic Conference on Biosensors, 2–17 November 2020; Available online: https://iecb2020.sciforum.net/.
Proceedings 2020, 60(1), 60; https://doi.org/10.3390/IECB2020-07017
Published: 2 November 2020
(This article belongs to the Proceedings of The 1st International Electronic Conference on Biosensors)
Immunoassays are, at present, an important tool for diagnostics, drug development, and environmental monitoring. However, most immunoassays involve procedures that require many elements for their development. We introduce a novel biosensing platform based on fluorescence quenching caused by graphene oxide (GO) for the detection of Human-IgG and Prostate-Specific Antigen (PSA). We employ a single antibody for the capture and detection processes, avoiding washing steps. FITC fluorophore was conjugated with antibodies for H-IgG detection, whereas quantum dots were conjugated with antibodies for PSA detection. The simple biosensing platform consists of covering a 96-well microplate (with a polystyrene bottom) with GO. The graphene oxide adhesion is possible by way of electrostatic interactions between the plate surface modified with amino groups (positively charged) and the graphene oxide (negatively charged). This proposal showed an excellent response for the detection of Human-IgG, with acceptable precision (from 0.27% to 5%). The limit of detection reached for H-IgG was 3.35 ng mL-1. In the same manner, for PSA detection, the limit of detection reached was 0.02 ng mL-1 and the precision range was from 0.7% to 15.2%. Furthermore, this biosensing platform was demonstrated to operate with real samples of human urine doped with different concentrations of prostate-specific antigen.
Keywords: FRET; fluorescence; immunoassays; graphene; single-step biosensing platform FRET; fluorescence; immunoassays; graphene; single-step biosensing platform
MDPI and ACS Style

Ortiz-Riaño, E.J.; Avila-Huerta, M.D.; Mancera-Zapata, D.L.; Morales-Narváez, E. Quenching of Fluorescence Caused by Graphene Oxide as an Immunosensing Platform in a Microwell Plate Format. Proceedings 2020, 60, 60. https://doi.org/10.3390/IECB2020-07017

AMA Style

Ortiz-Riaño EJ, Avila-Huerta MD, Mancera-Zapata DL, Morales-Narváez E. Quenching of Fluorescence Caused by Graphene Oxide as an Immunosensing Platform in a Microwell Plate Format. Proceedings. 2020; 60(1):60. https://doi.org/10.3390/IECB2020-07017

Chicago/Turabian Style

Ortiz-Riaño, Edwin J., Mariana D. Avila-Huerta, Diana L. Mancera-Zapata, and Eden Morales-Narváez. 2020. "Quenching of Fluorescence Caused by Graphene Oxide as an Immunosensing Platform in a Microwell Plate Format" Proceedings 60, no. 1: 60. https://doi.org/10.3390/IECB2020-07017

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