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Open AccessAbstract

Detection of Stephanofilaria (Nematoda: Filariidae) in Buffalo Fly Lesions

1
Queensland Alliance for Agriculture & Food Innovation (QAAFI), The University of Queensland Australia, Brisbane/St. Lucia 4067, Australia
2
College of Public Health, Medical & Vet Sciences, James Cook University Australia, Townsville 4810, Australia
3
Department of Agriculture and Fisheries, Queensland, Brisbane 4102, Australia
*
Author to whom correspondence should be addressed.
Presented at the third International Tropical Agriculture Conference (TROPAG 2019), Brisbane, Australia, 11–13 November 2019.
Proceedings 2019, 36(1), 108; https://doi.org/10.3390/proceedings2019036108
Published: 20 February 2020
Haematobia irritans exigua, commonly known as buffalo fly (BF), causes economic losses of about AUD $100 million per annum to the Australian cattle industry in terms of decreased production and costs of control. Lesions associated with BF infestation range from raised, dry, alopecic, hyperkeratotic or scab encrusted to severe hemorrhagic areas of ulceration which represent a major animal welfare concern. BF transmits a filarial nematode, Stephanofilaria sp., which has been speculatively associated with BF lesion development. The existing literature indicates that the sensitivity of currently used diagnostic techniques to detect Stephanofilaria in skin lesions is low and that there is currently no sequence for Stephanofilaria available on GenBank. Our objective is to develop a PCR method to detect Stephanofilaria in BF lesions. Skin biopsies were collected from 10 freshly slaughtered cattle hides having obvious BF eye lesions. Samples were collected from the center and the edge of the BF lesion as well as from adjacent normal tissue. Each skin punch was cut into 5-6 slices and immersed in normal saline before incubation overnight at 22°C. Eight nematodes were recovered from the saline by microscopic examination and preserved in ethanol. Nematode DNA will be extracted using conventional extraction methods. Specific primers will be used to amplify the ITS regions of rDNA and coxI region of the mtDNA and the amplified DNA will be sequenced. Primers will be designed from these regions to detect the presence of Stephanofilaria and used in PCR studies to clarify the etiology and epidemiology of BF lesions.
Keywords: Stephanofilaria; buffalo fly lesion; etiology; epidemiology; skin biopsies Stephanofilaria; buffalo fly lesion; etiology; epidemiology; skin biopsies
MDPI and ACS Style

Naseem, M.N.; Tabor, A.; Raza, A.; Constantinoiu, C.; Morgan, J.; James, P. Detection of Stephanofilaria (Nematoda: Filariidae) in Buffalo Fly Lesions. Proceedings 2019, 36, 108.

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