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Prognostic Role of DNA Methylation Analysis from Oral Brushing in Oral Squamous Cell Carcinoma
 
 
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Extended Abstract

13-Gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Diagnostic Tool in the Follow-Up of Patients Surgically Treated for Oral Cancer †

by
Eleonora Morselli
1,*,
Roberto Rossi
1,
Luca Morandi
2,
Achille Tarsitano
3,
Andrea Gabusi
1,
Linda Sozzi
1,
Stesi Kavaja
1 and
Davide B Gissi
1
1
Department of Biomedical and Neuromotor Sciences, Section of Oral Sciences, University of Bologna, 40125 Bologna, Italy
2
Functional MR Unit, IRCCS Istituto delle Scienze Neurologiche di Bologna, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40139 Bologna, Italy
3
Department of Biomedical and Neuromotor Sciences, Section of Maxillo-facial Surgery at S. Orsola-Malpighi Policlinic, University of Bologna, 40138 Bologna, Italy
*
Author to whom correspondence should be addressed.
†
Presented at the XV National and III International Congress of the Italian Society of Oral Pathology and Medicine (SIPMO), Bari, Italy, 17–19 October 2019.
Proceedings 2019, 35(1), 32; https://doi.org/10.3390/proceedings2019035032
Published: 11 December 2019
(This article belongs to the Proceedings of The XV National and III International Congress of the Italian Society of Oral Pathology and Medicine)
Versions Notes

1. Introduction

Patients treated for Oral Squamous Cell Carcinoma (OSCC) showed a significant risk to develop a loco-regional relapse during follow-up period. In clinical practice the follow-up strategy for early detection of recurrence or second primary tumors still consists of periodic visual examination and palpation of the oral cavity during the 5-year aftercare period.
Our research group recently developed a non-invasive procedure to identify oral carcinomas at early stage starting from oral brushing, quantitatively measuring the DNA methylation level of a panel of 13 genes [1].
The procedure resulted highly related to the presence of a malignant process (sensitivity 96.6%, specificity 100%). This high accuracy stimulated us to apply our non-invasive diagnostic tool in a cohort of patients previously treated for Oral Squamous Cell Carcinoma. Aim of the study is to evaluate if 13-gene DNA methylation analysis by oral brushing may be a useful procedure to identify patients surgically treated for OSCC at risk of developing a secondary tumor.

2. Materials and Methods

The study population included 49 consecutive surgically treated OSCC. Oral brushing sample collection was performed during patient follow up almost 6 months after OSCC treatment, within the regenerative area after OSCC resection. In all brushing specimens the DNA methylation level of ZAP70, GP1BB, KIF1A, ITGA4, LINC00599, MIR193, MIR296, TERT, LRRTM1, NTM, EPHX3, FLI1 and PARP15 was evaluated by quantitative Bisulfite-Next Generation Sequencing (NGS). Each sample was defined positive or negative in relationship to a specific algorithm and cut-off value [1]. Positive scores in the regenerative area after OSCC resection were analyzed together with other histologic poor prognostic factors for any relationship with appearance of loco-regional relapse.

3. Results

16/49 brushing specimens collected 6 months after surgery from regenerative area after OSCC showed a positive score.
During follow up period 7/49 (14.3%) patients developed a secondary OSCC during follow-up period (mean follow-up: 18.9 months): 6/7 patients showed positive samples from regenerative areas. The presence of a positive score resulted the most powerful variable related to the appearance of locoregional relapse, greater than presence of perineural invasion detected in the surgical OSCC sample.

4. Conclusions

These preliminary results seem to indicate that our novel assay may be proposed as an indicator of disease before appearance of clinical signs and symptoms in surgically treated OSCC patients. Further studies with larger cohort of patients, with adequate follow-up period and brushing sampling collection at different moments are needed to elucidate the prognostic potential of our assay.

Conflicts of Interest

The authors declare no conflict of interest

Reference

  1. Morandi, L.; Gissi, D.; Tarsitano, A.; Asioli, S.; Gabusi, A.; Marchetti, C.; Montebugnoli, L.; Foschini, M.P. CpG location and methylation level are crucial factors for the early detection of oral squamous cell carcinoma in brushing samples using bisulfite sequencing of a 13-gene panel. Clin. Epigenet. 2017, 9, 85. [Google Scholar]

Share and Cite

MDPI and ACS Style

Morselli, E.; Rossi, R.; Morandi, L.; Tarsitano, A.; Gabusi, A.; Sozzi, L.; Kavaja, S.; Gissi, D.B. 13-Gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Diagnostic Tool in the Follow-Up of Patients Surgically Treated for Oral Cancer. Proceedings 2019, 35, 32. https://doi.org/10.3390/proceedings2019035032

AMA Style

Morselli E, Rossi R, Morandi L, Tarsitano A, Gabusi A, Sozzi L, Kavaja S, Gissi DB. 13-Gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Diagnostic Tool in the Follow-Up of Patients Surgically Treated for Oral Cancer. Proceedings. 2019; 35(1):32. https://doi.org/10.3390/proceedings2019035032

Chicago/Turabian Style

Morselli, Eleonora, Roberto Rossi, Luca Morandi, Achille Tarsitano, Andrea Gabusi, Linda Sozzi, Stesi Kavaja, and Davide B Gissi. 2019. "13-Gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Diagnostic Tool in the Follow-Up of Patients Surgically Treated for Oral Cancer" Proceedings 35, no. 1: 32. https://doi.org/10.3390/proceedings2019035032

APA Style

Morselli, E., Rossi, R., Morandi, L., Tarsitano, A., Gabusi, A., Sozzi, L., Kavaja, S., & Gissi, D. B. (2019). 13-Gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Diagnostic Tool in the Follow-Up of Patients Surgically Treated for Oral Cancer. Proceedings, 35(1), 32. https://doi.org/10.3390/proceedings2019035032

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