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Proceedings 2018, 2(3), 128; https://doi.org/10.3390/ecsa-4-04915

Standalone Point-of-Use Device for Gluten Detection in Food: POCT Application Experiment in SMARTER-SI European Project

1
IK4-Ikerlan Technology Research Centre, Pº J.Mª Arizmendiarrieta 2, 20500 Arrasate-Mondragón, Gipuzkoa, Spain
2
Ingenasa, c/Hermanos García Noblejas 39, 28037 Madrid, Spain
3
Hahn-Schickard, Wilhelm-Schickard Str.10, 78052 Villingen-Schwenningen, Baden-Wurtemberg, Germany
4
CiS Research Institute for Microsensors GmbH, Konrad-Zuse-Straße 14, 99099 Erfurt, Germany
*
Author to whom correspondence should be addressed.
Presented at the 4th International Electronic Conference on Sensors and Applications, 15–30 November 2017; Available online: http://sciforum.net/conference/ecsa-4.
Published: 14 November 2017
PDF [1249 KB, uploaded 17 January 2018]

Abstract

In this work, we present an automatic and standalone microfluidic-based immunosensor to detect food allergens for food safety. The system has been developed as an application experiment in the SMARTER-SI European project. It comprises a microfluidic cartridge fabricated in cyclic olefin polymer by injection molding, the fluidic components to drive the reagents, two alternative optical detection systems based on fluorescent or colorimetric detection, respectively, and the electronics to manage all the components. As a proof-of-concept, gluten was immunodetected in a sandwich format by using R5 monoclonal antibody as capturing and detector probe. Gluten -antibody binding was revealed by using R5 mAb labelled to Alexa Fluor 647 or conjugated to horseradish peroxidase (HRP) for the fluorescence or the colorimetric version, respectively。
Keywords: gluten; SMARTER-SI; POCT; food; colorimetry; fluorescence gluten; SMARTER-SI; POCT; food; colorimetry; fluorescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Elizalde, J.; Eguiluz, X.; Mayora, K.; Rivas, L.A.; Sanz, A.; Dehnert, J.; Goridko, V.; Görlandt, S.; Schädel, M. Standalone Point-of-Use Device for Gluten Detection in Food: POCT Application Experiment in SMARTER-SI European Project. Proceedings 2018, 2, 128.

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