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Proceedings 2018, 2(25), 1528; https://doi.org/10.3390/proceedings2251528

Hydroxycinnamic Acids in Wild Blueberry and Effects of Hydroxycinnamic Acids on Apoptosis Induction in Cancer Cell Culture

1
Medical Biochemistry Department, Medical Faculty, Ege University, 35040 İzmir, Turkey
2
Medical Biology Department, Medical Faculty, Ege University, 35040 İzmir, Turkey
3
Department of Food Engineering, Faculty of Engineering, Ege University, 35040 İzmir, Turkey
Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018.
*
Author to whom correspondence should be addressed.
Published: 7 December 2018
PDF [218 KB, uploaded 7 December 2018]

Abstract

: In this study, the fruits and leaves of wild blueberries grown naturally in our country will be evaluated by using different infusion and boiling methods. Blueberry teas; leaves, raw fruit, dried and shredded raw fruit, fruit beans and seedless raw fruit of different infusions were used after boiling them for 1 min, 3 min, 5 min, 7 min, 10 min. Phenolic levels were determined by LC MS/MS technique. The antioxidant and activities of all products in the vitro HCT-116 colon cancer cell line were analyzed by spectrophotometric methods. MDA and TEAC were evaluated for antioxidant activity. Cytotoxicity and viability tests were performed by adding WST-8 (Water Soluble Tetrazolium Salt-8) solution. For apoptosis, TRAIL and Apaf-1 ELISA Kit were used for the activation of caspases of intrinsic and extrinsic pathways.
Keywords: blueberries; apoptosis; colon cancer; Apaf-1; TRAIL blueberries; apoptosis; colon cancer; Apaf-1; TRAIL
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Durmaz, B.; Oktay, L.M.; Mehmedov, H.; Günel, N.S.; Yıldırım, H.K.; Sözmen, E.Y. Hydroxycinnamic Acids in Wild Blueberry and Effects of Hydroxycinnamic Acids on Apoptosis Induction in Cancer Cell Culture. Proceedings 2018, 2, 1528.

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