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Proceedings 2018, 2(25), 1524; https://doi.org/10.3390/proceedings2251524

Effects of Quercetin and Curcumin Combination on Signal Transduction Pathways in Chronic Myeloid Leukemia Cells

1
Department of Biochemistry, Faculty of Medicine, Eastern Mediterranean University, Famagusta 99628, Cyprus
2
Department of Biochemistry, School of Medicine Marmara University, Istanbul 34854, Turkey
3
Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, Istanbul 34854, Turkey
4
Department of Biochemistry, Faculty of Medicine, Dokuz Eylül University, Izmir 35330, Turkey
Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018.
*
Author to whom correspondence should be addressed.
Published: 7 December 2018
PDF [1091 KB, uploaded 7 December 2018]

Abstract

Flavonoids have chemo-preventive and chemotherapeutic properties against different human cancers including chronic myeloid leukemia. Quercetin and curcumin are two polyphenols with potential anti-carcinogenic and pro-apoptotic properties. We have previously demonstrated the synergistic protective effect of quercetin and curcumin on chronic myeloid leukemia cells (K562) cells. Anti-proliferative and apoptotic effects of these polyphenols were examined by apoptosis and cell viability assays. Oxidative status of the cells was analyzed by determining the level of reactive oxygen species, mitochondrial permeability and intracellular glutathione. Obtained data showed that quercetin and curcumin had beneficial and synergistic effects on K562 cells. On the basis of the above-mentioned data, herein we aimed to clarify signaling pathways involved in synergistic combination of quercetin and curcumin on K562 cells. Normal peripheral blood mononuclear cell line was used as controls. The mRNA and protein expressions of the signaling pathways were detected by Human Signal Transduction Pathway Finder-RT2 PCR Array system and Western blotting, respectively. The results of PCR array were evaluated by DAVID v6.8 and database for KEGG pathways. Our data revealed that synergistic combination of curcumin quercetin was effective on genes that were particularly related to P53, NF and TGF. We believe that our findings will lead to new research in this area and will contribute to the chronic myeloid leukemia treatment protocols.
Keywords: chronic myeloid leukemia (CML), quercetin; curcumin; apoptosis; signal transduction chronic myeloid leukemia (CML), quercetin; curcumin; apoptosis; signal transduction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Altundağ, E.M.; Yılmaz, A.M.; Sert, B.; Erkmen, T.; Koçtürk, S.; Yalçın, A.S. Effects of Quercetin and Curcumin Combination on Signal Transduction Pathways in Chronic Myeloid Leukemia Cells. Proceedings 2018, 2, 1524.

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