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Proceedings 2018, 2(13), 920; https://doi.org/10.3390/proceedings2130920

Fast Formation of Lipid Bilayer Membranes for Simultaneous Analysis of Molecular Transport Using Parylene Coated Chips

1
Institute for Microsensors, Microactuators and Microsystems (IMSAS), University of Bremen, Microsystems Center Bremen (MCB), D-28359 Bremen, Germany
2
Ionovation GmbH, D-49143 Bissendorf, Germany
3
Department of Life Sciences & Chemistry, Jacobs University, D-28759 Bremen, Germany
Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018.
*
Author to whom correspondence should be addressed.
Published: 23 November 2018
(This article belongs to the Proceedings of Eurosensors 2018)
PDF [710 KB, uploaded 23 November 2018]

Abstract

Artificial lipid bilayers are an essential tool to investigate channel forming proteins. A particular challenge is to study antibiotic uptake through bacterial porins requiring low volume and parallelization. Here, we present a lipid bilayer silicon chip having a Parylene-C coated silicon nitride membrane with different aperture sizes. The Parylene-C allows very fast lipid bilayer membrane fabrication, 30 to 130 s. The realization-success is very high and an average lifetime of at least 9 h was established. Furthermore, a 3D-printed holder is realized where parallel assembly of the chips, including fluid inlets for the pipetting robot, is demonstrated.
Keywords: Parylene coating; 3D printed holder; lipid bilayer chips, microfluidics, silicon nitride membrane Parylene coating; 3D printed holder; lipid bilayer chips, microfluidics, silicon nitride membrane
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ahmed, T.; Driesche, S.; Oellers, M.; Hemmler, R.; Gall, K.; Bhamidimarri, S.P.; Winterhalter, M.; Wagner, R.; Vellekoop, M.J. Fast Formation of Lipid Bilayer Membranes for Simultaneous Analysis of Molecular Transport Using Parylene Coated Chips. Proceedings 2018, 2, 920.

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